Lecture - Gastrointestinal Development
|Embryology - 19 Oct 2019 Expand to Translate|
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- 1 Endoderm Development
- 1.1 Introduction
- 1.2 Lecture Objectives
- 1.3 Lecture Resources
- 1.4 Germ Layer Contributions
- 1.5 Week 4
- 1.6 Week 5
- 1.7 Week 8 - 10
- 1.8 Gastrointestinal Tract Divisions
- 1.9 Fetal
- 1.10 Liver
- 1.11 Pancreas
- 1.12 Spleen
- 1.13 Enteric Nervous System
- 1.14 Gastrointestinal Tract Abnormalities
- 1.15 References
- 1.16 Terms
- 1.17 Glossary Links
This lecture will cover the early development of the endoderm layer of the trilaminar embryo as it contributes to the lining, glands and organs of the gastrointestinal tract (GIT). The endoderm contribution to the respiratory system will be covered in a separate lecture.
Gastrulation, or gut formation, was historically the easiest observable feature of frog development. In human development, during the 4th week the 3 distinct portions (fore-, mid- and hind-gut) extend the length of the embryo and will contribute different structures.
The oral cavity (mouth) is formed following breakdown of the buccopharyngeal membrane (= oropharyngeal or oral) and the opening means that it contains amniotic fluid, which is also swallowed later in development.
The large mid-gut is generated by lateral embryonic folding which "pinches off" a pocket of the yolk sac, the 2 compartments continue to communicate through the vitelline duct.
The hindgut (cloaca) will later be divided into separate urogenital and rectal regions that end at the cloacal membrane.
Note that we will be returning in the laboratory and later (head, endocrine, neural crest) to discuss the gastrointestinal tract, associated organs and physical growth changes.
|Some Recent Articles|
Castillo-Azofeifa D, Seidel K, Gross L, Golden EJ, Jacquez B, Klein OD & Barlow LA. (2018). SOX2 regulation by hedgehog signaling controls adult lingual epithelium homeostasis. Development , 145, . PMID: 29945863 DOI.
Cobourne MT, Iseki S, Birjandi AA, Adel Al-Lami H, Thauvin-Robinet C, Xavier GM & Liu KJ. (2018). How to make a tongue: Cellular and molecular regulation of muscle and connective tissue formation during mammalian tongue development. Semin. Cell Dev. Biol. , , . PMID: 29784581 DOI.
Carnegie stage 14 (CS14) orientation of the midgut loop and its mesentery during the 5th week follows the helical body axis.
- Understanding of germ layer contributions to the early gastrointestinal tract (GIT)
- Understanding of the folding of the GIT
- Understanding of three main GIT embryonic divisions
- Understanding of associated organ development (liver, pancreas, spleen)
- Brief understanding of mechanical changes (rotations) during GIT development
- Brief understanding of gastrointestinal abnormalities
Week 4-5 Stage 13
Week 8 Stage 22
|2015 Lecture - Video and Audio|
Germ Layer Contributions
- endoderm - epithelium and associated glands.
- mesoderm (splanchnic) - mesentry, connective tissues, smooth muscle, blood vessels.
- ectoderm (neural crest) - enteric nervous system.
Both endoderm and mesoderm will also have major contributions to associated organs.
Folding of the embryonic disc occurs ventrally around the notochord, which forms a rod-like region running rostro-caudally in the midline.
In relation to the notochord:
- Laterally (either side of the notochord) lies mesoderm.
- Rostrally (above the notochord end) lies the buccopharyngeal membrane, above this again is the mesoderm region forming the heart.
- Caudally (below the notochord end) lies the primitive streak (where gastrulation occurred), below this again is the cloacal membrane.
- Dorsally (above the notochord) lies the neural tube then ectoderm.
- Ventrally (beneath the notochord) lies the mesoderm then endoderm.
The ventral endoderm (shown yellow) has grown to line a space called the yolk sac. Folding of the embryonic disc "pinches off" part of this yolk sac forming the first primative GIT.
- The mesoderm initially undergoes segmentation to form paraxial, intermediate mesoderm and lateral plate mesoderm.
- Paraxial mesoderm segments into somites and lateral plate mesoderm divides into somatic and splanchnic mesoderm.
- The space forming between them is the coelomic cavity, that will form the 3 major body cavities (pericardial, pleural, peritoneal)
- Most of the gastrointestinal tract will eventually lie within the peritoneal cavity.
(Note only the righhand side is shown, lefthand side would be identical.)
(Gestational age GA 6 weeks) Carnegie stage 11
|Embryo (stage 11 ventral view)||Embryo (midline section)||Embryo (EM section) endoderm, splanchnic mesoderm, Intraembryonic coelom|
liver contributions from endoderm and splanchnic mesoderm at the level of the transverse septum (week 4)
- Stage 11 - hepatic diverticulum development
- Stage 12 - cell differentiation, septum transversum forming liver stroma, hepatic diverticulum forming hepatic trabeculae
- Stage 13 - epithelial cord proliferation enmeshing stromal capillaries
The liver initially occupies the entire anterior body. All blood vessels enter the liver (placental, vitelline) and leave to enter the heart.
- During week 4 at the level where the stomach will form the tube begins to dilate, forming an enlarged lumen.
- The dorsal border grows more rapidly than ventral first rotation (of 90 degrees), which establishes the greater curvature of the stomach.
- A second rotation (of 90 degrees) occurs on the longitudinal axis establishing the adult orientation of the stomach.
(GA 7 weeks)
Week 8 - 10
(GA 10-12 weeks)
Gastrointestinal Tract Divisions
|During the 4th week the 3 distinct portions (fore-, mid- and hind-gut) extend the length of the embryo and will contribute different components of the GIT. These 3 divisions are also later defined by the vascular (artery) supply to each of theses divisions.
Gastrointestinal Tract Blood Supply
|Small Intestine length (mm)||Liver Growth (weight grams)|
|1 to 124 grams (birth)|
- Differentiates to form the hepatic diverticulum and hepatic primordium, generates the gall bladder then divides into right and left hepatic (liver) buds.
- Hepatic Buds - form hepatocytes, produce bile from week 13 (forms meconium of newborn)
- Left Hepatic Bud - left lobe, quadrate, caudate (both q and c anatomically Left) caudate lobe of human liver consists of 3 anatomical parts: Spiegel's lobe, caudate process, and paracaval portion.
- Right Hepatic Bud - right lobe
- Bile duct - 3 connecting stalks (cystic duct, hepatic ducts) which fuse.
- Early liver also involved in blood formation, after the yolk sac and blood islands acting as a primary site.
- Pancreatic buds - endoderm, covered in splanchnic mesoderm
- Pancreatic bud formation – duodenal level endoderm, splanchnic mesoderm forms dorsal and ventral mesentery, dorsal bud (larger, first), ventral bud (smaller, later)
- Duodenum growth/rotation – brings ventral and dorsal buds together, fusion of buds, exocrine function (postnatal function)
- Pancreatic duct – ventral bud duct and distal part of dorsal bud
- Pancreatic islets - endocrine function (week 10 onwards)
Enteric Nervous System
Topic covered in the neural crest lecture. Table below summarises the two major neural plexuses.
|Myenteric plexus||Submucosal plexus|
|Auerbach's plexus||Meissner's plexus|
|Leopold Auerbach (1828–1897) a German anatomist and neuropathologist.||Georg Meissner (1829–1905) a German anatomist and physiologist.|
|Links: enteric nervous system | intestine | neural crest | PMID 25428846|
Gastrointestinal Tract Abnormalities
|There are several types of abnormalities that impact upon the continuity of the gastrointestinal tract lumen.
|Presents clinically in symptomatic malrotation as:
|(intestinal aganglionosis, Hirschsprung's disease, aganglionic colon, megacolon, congenital aganglionic megacolon, congenital megacolon)
|Gastroschisis (omphalocele, paraomphalocele, laparoschisis, abdominoschisis, abdominal hernia) is a congenital abdominal wall defect which results in herniation of fetal abdominal viscera (intestines and/or organs) into the amniotic cavity.
Incidence of gastroschisis has been reported at 1.66/10,000, occuring more frequently in young mothers (less than 20 years old).
By definition, it is a body wall defect, not a gastrointestinal tract defect, which in turn impacts upon GIT development.
This indirect developmental effect (one system impacting upon another) occurs in several other systems.
Final Thoughts- After Birth
Remember that the GIT does not function until after birth consider:
- metabolic disorders discovered by neonatal diagnosis
- Neonatal feeding difficulties due to cleft lip and cleft palate.
Expand the table below to see GIT related terms.
|Gastrointestinal Tract Terms|
- Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link
|2018 ANAT2341 - Timetable | Course Outline | Moodle | Tutorial 1 | Tutorial 2 | Tutorial 3|
Labs: 1 Preimplantation and Implantation | 2 Reproductive Technology Revolution | 3 Group Projects | 4 GM manipulation mouse embryos | 5 Early chicken eggs | 6 Female reproductive tract | 7 Skin regeneration | 8 Vertebral development | 9 Organogenesis Lab | 10 Cardiac development | 11 Group projects | 12 Stem Cell Journal Club
Lectures: 1 Introduction | 2 Fertilization | 3 Week 1/2 | 4 Week 3 | 5 Ectoderm | 6 Placenta | 7 Mesoderm | 8 Endoderm | 9 Research Technology | 10 Cardiovascular | 11 Respiratory | 12 Neural crest | 13 Head | 14 Musculoskeletal | 15 Limb | 16 Renal | 17 Genital | 18 Endocrine | 19 Sensory | 20 Fetal | 21 Integumentary | 22 Birth | 23 Stem cells | 24 Revision
|Student Projects: Group Projects Information Project 1 | Project 3 | Project 4 | Project 5 | 2018 Test Student | Copyright|
Cite this page: Hill, M.A. (2019, October 19) Embryology Lecture - Gastrointestinal Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Lecture_-_Gastrointestinal_Development
- © Dr Mark Hill 2019, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G