Carnegie stage 15

Embryology - 5 Dec 2023 Expand to Translate
Google Translate - select your language from the list shown below (this will open a new external page)
العربية \| català \| 中文 \| 中國傳統的 \| français \| Deutsche \| עִברִית \| हिंदी \| bahasa Indonesia \| italiano \| 日本語 \| 한국어 \| မြန်မာ \| Pilipino \| Polskie \| português \| ਪੰਜਾਬੀ ਦੇ \| Română \| русский \| Español \| Swahili \| Svensk \| ไทย \| Türkçe \| اردو \| ייִדיש \| Tiếng Việt These external translations are automated and may not be accurate. (More? About Translations)

Introduction

Facts

Facts: Week 5, 35 - 38 days, 7 - 9 mm

Gestational Age GA week 7

Summary

Ectoderm: sensory placodes, lens pit, otocyst, nasal pit, primary/secondary vesicles, fourth ventricle of brain,
Mesoderm: heart prominence
Head: 1st, 2nd and 3rd pharyngeal arch, forebrain, site of lens placode, site of otic placode, stomodeum
Body: heart, liver, umbilical cord, mesonephric ridge
Limb: upper and lower limb buds, hand plate

Features

Identify: midbrain region, nasal pit, lens pit, 1st, 2nd and 3rd pharyngeal arches, 1st pharyngeal groove, maxillary and mandibular components of 1st pharyngeal arch, fourth ventricle of brain, heart prominence, cervical sinus, upper limb bud, mesonephric ridge, lower limb bud, umbilical cord Labelled Stage 15

Historic Papers: 1891 | 1905 Cranial and Spinal Nerves | 1914 | 1926 Precervical Sinus | 1948 stages 15-18

Week:	1	2	3	4	5	6	7	8
Carnegie stage:	1 2 3 4	5 6	7 8 9	10 11 12 13	14 15	16 17	18 19	20 21 22 23

Carnegie Stages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | About Stages | Timeline

Kyoto Collection

Embryo Lateral view. Amniotic membrane removed.

Embryo Stage 15 (7207) external view
right
ventral
left
dorsal
left head label

Embryo Sagittal section - upper half

Image source: The Kyoto Collection images are reproduced with the permission of Prof. Kohei Shiota and Prof. Shigehito Yamada, Anatomy and Developmental Biology, Kyoto University Graduate School of Medicine, Kyoto, Japan for educational purposes only and cannot be reproduced electronically or in writing without permission.

Carnegie Collection

Historic Papers: 1891 | 1905 Cranial and Spinal Nerves | 1914 | 1926 Precervical Sinus | 1948 stages 15-18

Week:	1	2	3	4	5	6	7	8
Carnegie stage:	1 2 3 4	5 6	7 8 9	10 11 12 13	14 15	16 17	18 19	20 21 22 23

Carnegie Collection Embryos - Stage 15
Serial No.	Size (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Year	Notes
2	E., 7.0 Ch., 25x25x25	Good	Alc.	P	Transverse	15	Al. carm.	1888	Least-advanced third
88	E.,8 Ch., 30x28x15	Poor	Alc.	P	Coronal	P	Al. coch.	1897
113	E.,8 241 E,6.0	Poor	P	p	Sagittal	10	Borax carmine	?
241	E,6.0	Good	Formalin	P	Transverse	10	H. & Congo red	1904
371	E,6.6	Good	Formalin	P	Sagittal	10	Al. coch.	1913	Shrunken and cracked
389	E., 9	Poor	p	p	Sagittal	20	(Stain - Haematoxylin Eosin)	1907	Tubal
721	E., 9.0 Ch., 30x20x10	Exc.	Zenker formol	P	Transverse	15	(Stain - Haematoxylin Eosin)	1913	Median in group
810	E., 7.0 Ch, 30x25x15	Good	Alc.	P	Sagittal	20	Al. coch	1913
855	E.,7.5	Poor	Formalin	P	Transverse	100	Al. coch.	1914	Pathological between limbs
1006	E,9.0 Ch., 37x26x22	Poor	Formalin	P	Coronal	20	(Stain - Haematoxylin Eosin) or. G.	1914	Operative. Most-advanced third
1091	E,7.2 Ch., 28x26x20	Poor	P	P	Coronal	20	Al. coch.	1915	Macerated
1354	E,7.8 Ch, 35x30x25	Good	Formalin	P	Sagittal	20	Al. coch.	1916	Least-advanced third
1767	E , 11.0 Ch, 41x23x5	Good	Formalin	P	Sagittal	40	(Stain - Haematoxylin Eosin) or. G.	1917	Most-advanced third
2743	E., 7.2 Ch., 19xl8x14	Poor	Formalin	P	Transverse	20	Al. coch.	1919	Macerated. Least-advanced third
3216	E, 6.5 Ch, 30x30x5	Good	Formalin	P	Transverse	20	Al. coch.	1920	Hysterectomy. Least-advanced third
3385	E,83 Ch., 25x20x16	Exc.	Corros. acetic	P	Trans.	20	(Stain - Haematoxylin Eosin) or. G.	1921	Some sections lost. Most-advanced third. Ag added
3441	E,8.0 Ch., 25x24x20	Good	Formalin	P	Sag.	10	Al. coch.	1921
3512	E,8,5 Ch., 33x28x25	Good	Formalin	P	Trans.	10	Al. coch.	1921
3952	E,6,7 Ch., 30x25x15	Good	Formalin	P	Cor.	15	Al. coch.	1922	Median in group
4602	E,9.3 Ch,, 33x30x26	Good	Formalin	P	Sag.	15	Al. coch.	1924	Medical abortion
4782	E,9.0 Ch., I4xl3x11	Poor	Formalin	P	Cor.	20	Al. coch.	1924
5772	E, 8	Poor	?	P	Cor.	15	Al. coch. eosin	1928
Template:CE5?92	E, 3	Good	Corros. acetic	C-P	Cor.	10	Al. coch. phlox	1929	Transitional to next stage
6223	E ?	Poor	Alc.	C-P	Sag.	8	Or. G.	1930	Fragmented sections. Not saved
6504
6506
6508
6595
????
Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined.

iBook - Carnegie Embryos
	iTunes link \| iBook Store Description - Imagine the excitement of seeing this incredible early period of human development for the first time. Now consider that much of our initial understanding of human development is based upon study of historic embryo collections. You can now look at these historic images of the first 8 weeks after fertilisation and explore for yourself the changes that occur in human development during this key period. This current book is designed as an atlas of the Carnegie embryo stages with some brief notes and additional information covering the first 8 weeks of development. These images are from from the beginning of last century and are one of the earliest documented series of human embryos collected for basic research and medical education on development. I hope you enjoy learning about the amazing early events that begin to make and shape us. This is the second book in a series of educational releases from UNSW Embryology. Release: First Edition - Mar 12, 2012 ISBN 978-0-7334-3148-7 Print Length 82 Pages, 25.8 MB Language English. PDF Preview version 3.87 MB (Read the associated information, this is an edited educational preview version with many features not functioning). The current website also includes numerous embryo images from this textbook (see Embryonic Development and Carnegie Embryos).

Blechschmidt Collection

‎‎Embryo 7.5mm

Page | Play

left lateral
left ventrolateral
left ventral
ventral
right ventral
right lateral
right dorsolateral
right dorsal
dorsal
left dorsolateral

Image source: The Blechschmidt Collection images are reproduced with the permission of Prof. Christoph Viebahn, director of the Institute of Anatomy and Embryology, , University Medical Center Göttingen. Images are for educational purposes only and cannot be reproduced electronically or in writing without permission.

Madrid Collection

Madrid Collection Embryos
Carnegie Stage	Embryo	Days	CRL (mm)	Section thickness	Staining	Section plane
15	GV4	36	7	8	(Stain - Haematoxylin Eosin)	transverse

Photograph

Image: Dr Ed Uthman (Houston, Texas) - other pathology images

Image version links: Virtual Slide | ExtraLarge 1874 x 2000px | Large 959 x 1024px | Medium 468 x 500px

Embryo Virtual Slide

Stage 15 - Ventral View

‎‎Mobile | Desktop | Original

Stage 15 | Embryo Slides

Historic Stage 15 Embryos

Carnegie No. 2

Carnegie No. 2, 7 mm. Described in detail by Mall (1891).^[1]
Hochstetter's embryo I, 7 mm. ( (No. 10 of Hochstetter's series) Described in monographic form by Elze (1907).^[2]Includes illustrations of reconstructions. Also shown in Fig. 52 of Keibel and Mall (1910).^[3]
Legg embryo, 7 mm. Described in detail by Thompson (1915).^[4]Includes illustrations of reconstructions.
8-mm embryo. The peripheral nervous system was described by Volcher (1963)^[5] in this embryo of stage 15.
Keibel No. 1495, 8.5 mm. This advanced example of stage 15 was well described in detail by Barniville (1914)^[6].
Blechschmidt 7.5 mm Blechschmidt Model generated from serial sections of this embryo.
Newcastle N340 Optical Projection Tomography of this embryo described by J. Kerwin etal., (2004).^[7]

Events

hearing - otic capsule formed by condensed mesenchyme. Ganglion vestibular nerve fibres extend to the otocyst epithelium. External ear auricular hillocks appear, pharyngeal arch 2 forms primordium of the antitragus.
vision - (about 33 days) the lens pit is closed. The lens vesicle and optic cup lie close to the surface ectoderm and appear to press against the surface.^[8]
smell - the nasal discs form large oval depressions (nasal pits). A continuous cellulovascular strand is observed between the nasal groove and the olfactory field.^[9] Stages Description
limb - upper limb bud visible as a distal hand plate and a proximal forearm, arm, and shoulder region.
somitogenesis - last stage that somites can be seen from the surface.
cardiovascular
- heart - atrioventricular bundle appears, atrioventricular cushions present, conotruncal ridges present, foramen secundum appears and semilunar cusps appear.^[10]
- Coronary circulation plexus of blind epicardial capillaries appears on the heart begin to acquire coronary sinus connection.^[11]
- Cerebral artery vertebral arteries forms from transverse anastomoses between cervical intersegmental arteries, beginning with the proatlantal artery and proceeding downward to the 6th intersegmental artery.^[12]
endocrine^[13]
- Epiphysis - pineal body is detectable in the roof of the diencephalon (Stadium I of Turkewitsch 1933) (O'Rahilly 1968).
- thyroid - thyroid primordium may be detached from the pharyngeal epithelium in some instances. "At about the time" when the thyroglossal duct "becomes broken it loses its lumen" (Grosser 1912).
- Adrenal Cortex - primordium is first recognizable. A new type of cell (C1) from the coelomic epithelium is found in the subjacent mesenchyme. New cells (C2) appear in the medial wall of mesonephric glomeruli and begin to migrate into the suprarenal primordium.^[14] Jirfisek (1980) denies a mesonephric contribution to the suprarenal.
- Adrenal Medulla - all types of cells (M1, M2, and M3) increase in number. From stage 15 to stage 18, the suprarenal primordium is cigar-shaped and extends from segment T6 to segment L1, lateral to the aorta and mesogastrium.^[14]
Meninges (Spinal Cord) - vertebral rudiments now form concentrations that are distinct from the intermediate zone separating them from the neural tube. This intermediary zone, which is less densely cellular and within which lie the ganglia, is the meninx primitiva, from which the meninges have been generally considered to arise. The neural tube is now completely surrounded by endothelium-lined vascular channels. Directly adjacent to the cord, and particularly evident at its ventrolateral surfaces, is a single-layered cell membrane. This is the ﬁrst indication of the pia mater, and, where it is present, it lies between the vascular channels and the periphery of the spinal cord.^[15]
ventral body wall - medially vertebrae and ribs formed, and primordia of sternum and hypaxial flank muscle primordium laterally in the body wall.^[16]

References

↑ Mall FP. A human embryo twenty-six days old. (1891) J Morphol. 5: 459-480.
↑ Elze, C. 1907. Beschreibung eines menschlichen Embryo von zirka 7 mm grosster Lange unter besonderer Beriicksichtigung der Frage nach der Enrwickelung der Extremitatenarterien und nach der morphologischen Bedeutung der lateralen Schilddriisenanlage. (Description of a human embryo of about 7 mm greatest length with special reference to the question of the development of the extremities arteries and to the morphological importance of lateral shield primordia). Anat. Hefte, 106, 411-492.
↑ Keibel F. and Mall FP. Manual of Human Embryology I. (1910) J. B. Lippincott Company, Philadelphia.
↑ Thompson P. Description of a human embryo, 7 mm. greatest length. (1915) Studies in Anatomy. University of Birmingham, pp. 1-50.
↑ Volcher, R. 1963. Le systeme nerveux périphérique d'un embryon humain de 8 mm.(The peripheral nervous system of an 8 mm. human embryo) Arch. Biol. (Liege), 74, 95-127. PMID 13997757
↑ Barniville HL. The morphology and histology of a human embryo of 8.5 mm. (1914) J Anat. Physiol. 49(1):1-71. PMID 17233012
↑ Kerwin J, Scott M, Sharpe J, Puelles L, Robson SC, Martínez-de-la-Torre M, Ferran JL, Feng G, Baldock R, Strachan T, Davidson D & Lindsay S. (2004). 3 dimensional modelling of early human brain development using optical projection tomography. BMC Neurosci , 5, 27. PMID: 15298700 DOI.
↑ Pearson AA. (1980). The development of the eyelids. Part I. External features. J. Anat. , 130, 33-42. PMID: 7364662
↑ Bossy J. Development of olfactory and related structures in staged human embryos. (1980) Anat. Embryol., 161(2);225-36 PMID 7469043
↑ Arráez-Aybar LA, Turrero-Nogués A & Marantos-Gamarra DG. (2008). Embryonic cardiac morphometry in Carnegie stages 15-23, from the Complutense University of Madrid Institute of Embryology Human Embryo Collection. Cells Tissues Organs (Print) , 187, 211-20. PMID: 18057862 DOI.
↑ Hutchins GM, Kessler-Hanna A & Moore GW. (1988). Development of the coronary arteries in the embryonic human heart. Circulation , 77, 1250-7. PMID: 3286038
↑ Menshawi K, Mohr JP & Gutierrez J. (2015). A Functional Perspective on the Embryology and Anatomy of the Cerebral Blood Supply. J Stroke , 17, 144-58. PMID: 26060802 DOI.
↑ O'Rahilly R. The timing and sequence of events in the development of the human endocrine system during the embryonic period proper. (1983) Anat. Embryol., 166: 439-451. PMID 6869855
↑ ^14.0 ^14.1 Crowder RE. The development of the adrenal gland in man, with special reference to origin and ultimate location of cell types and evidence in favor of the "cell migration" theory. (1957) Contrib. Embryol., Carnegie Inst. Wash. 36, 193-210.
↑ Sensenig EC. The early development of the meninges of the spinal cord in human embryos. (1951) Contrib. Embryol., Carnegie Inst. Wash. Publ. 611.
↑ Mekonen HK, Hikspoors JP, Mommen G, Köhler SE & Lamers WH. (2015). Development of the ventral body wall in the human embryo. J. Anat. , 227, 673-85. PMID: 26467243 DOI.