Carnegie stage 15

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Introduction

Stage15 bf1c.jpg

Facts

Facts: Week 5, 35 - 38 days, 7 - 9 mm

Gestational Age GA week 7

Summary

  • Ectoderm: sensory placodes, lens pit, otocyst, nasal pit, primary/secondary vesicles, fourth ventricle of brain,
  • Mesoderm: heart prominence
  • Head: 1st, 2nd and 3rd pharyngeal arch, forebrain, site of lens placode, site of otic placode, stomodeum
  • Body: heart, liver, umbilical cord, mesonephric ridge
  • Limb: upper and lower limb buds, hand plate

See also Carnegie stage 15 Events

Features

  • Identify: midbrain region, nasal pit, lens pit, 1st, 2nd and 3rd pharyngeal arches, 1st pharyngeal groove, maxillary and mandibular components of 1st pharyngeal arch, fourth ventricle of brain, heart prominence, cervical sinus, upper limb bud, mesonephric ridge, lower limb bud, umbilical cord Labelled Stage 15


Stage 15 Links: Week 5 | Head | Lecture - Limb | Lecture - Gastrointestinal | Lecture - Head Development | Science Practical - Gastrointestinal | Science Practical - Head | Carnegie Embryos | Category:Carnegie Stage 15 | Next Stage 16
  Historic Papers: 1891 | 1905 Cranial and Spinal Nerves | 1914 | 1926 Precervical Sinus | 1948 stages 15-18


Week: 1 2 3 4 5 6 7 8
Carnegie stage: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Carnegie Stages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | About Stages | Timeline

Kyoto Collection

Stage15 bf1.jpg

Embryo Lateral view. Amniotic membrane removed.


Stage15 sagittal section upper half 01.jpg

Embryo Sagittal section - upper half


Image source: The Kyoto Collection images are reproduced with the permission of Prof. Kohei Shiota and Prof. Shigehito Yamada, Anatomy and Developmental Biology, Kyoto University Graduate School of Medicine, Kyoto, Japan for educational purposes only and cannot be reproduced electronically or in writing without permission.

Carnegie Collection

Stage15 bf3.jpg Stage15 bf4.jpg Stage15 bf5.jpg Stage15 bf6.jpg

Stage15 bf7.jpg Stage15 bf8.jpg Stage15 bf9.jpg Stage15 bf10.jpg

Carnegie stage 15: Right | Anterior | Posterior | Left | Right | Anterior | Posterior | Left


Stage 15 Links: Week 5 | Head | Lecture - Limb | Lecture - Gastrointestinal | Lecture - Head Development | Science Practical - Gastrointestinal | Science Practical - Head | Carnegie Embryos | Category:Carnegie Stage 15 | Next Stage 16
  Historic Papers: 1891 | 1905 Cranial and Spinal Nerves | 1914 | 1926 Precervical Sinus | 1948 stages 15-18


Week: 1 2 3 4 5 6 7 8
Carnegie stage: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23



Carnegie Collection - Stage 15 
Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
2 E., 7.0 Ch., 25x25x25 Good Alc. P Trans. 15 Al. carm. 1888 Least-advanced third
88 E.,8 Ch., 30x28x15 Poor Alc. P Cor. P Al. coch. 1897
113 E.,8 241 E,6.0 Poor P p Sag. 10 Borax carmine  ?
241 E,6.0 Good Formol P Trans. 10 H. & Congo red 1904
371 E,6.6 Good Formol P Sag. 10 Al. coch. 1913 Shrunken and cracked
389 E., 9 Poor p p Sag. 20 (Stain - Haematoxylin Eosin) 1907 Tubal
721 E., 9.0 Ch., 30x20x10 Exc. Zenker formol P Trans. 15 (Stain - Haematoxylin Eosin) 1913 Median in group
810 E., 7.0 Ch, 30x25x15 Good Alc. P Sag. 20 Al. coch 1913
855 E.,7.5 Poor Formol P Trans. 100 Al. coch. 1914 Pathological between limbs
1006 E,9.0 Ch., 37x26x22 Poor Formol P Cor. 20 (Stain - Haematoxylin Eosin) or. G. 1914 Operative. Most-advanced third
1091 E,7.2 Ch., 28x26x20 Poor P P Cor. 20 Al. coch. 1915 Macerated
1354 E,7.8 Ch, 35x30x25 Good Formol P Sag. 20 Al. coch. 1916 Least-advanced third
1767 E , 11.0 Ch, 41 X23X5 Good Formol P Sag. 40 (Stain - Haematoxylin Eosin) or. G. 1917 Most-advanced third
2743 E., 7.2 Ch., 19xl8x 14 Poor Formol P Trans. 20 Al. coch. 1919 Macerated. Least-advanced third
3216 E, 6.5 Ch, 30x30x5 Good Formol P Trans. 20 Al. coch. 1920 Hysterectomy. Least-advanced third
3385 E,83 Ch., 25x20x16 Exc. Corros. acetic P Trans. 20 (Stain - Haematoxylin Eosin) or. G. 1921 Some sections lost. Most-advanced third. Ag added
3441 E,8.0 Ch., 25x24x20 Good Formol P Sag. 10 Al. coch. 1921
3512 E,8,5 Ch., 33x28x25 Good Formol P Trans. 10 Al. coch. 1921
3952 E,6,7 Ch., 30x25x15 Good Formol P Cor. 15 Al. coch. 1922 Median in group
4602 E,9.3 Ch,, 33x30x26 Good Formol P Sag. 15 Al. coch. 1924 Medical abortion
4782 E,9.0 Ch., I4xl3x11 Poor Formol P Cor. 20 Al. coch. 1924
5772 E, 8 Poor  ? P Cor. 15 Al. coch. eosin 1928
Template:CE5?92 E, 3 Good Corros. acetic C-P Cor. 10 Al. coch. phlox 1929 Transitional to next stage
6223 E ? Poor Alc. C-P Sag. 8 Or. G. 1930 Fragmented sections. Not saved
6504
6506
6508
6595
 ????
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  •  ? - unknown or not determined.


iBook - Carnegie Embryos  
link=http://itunes.apple.com/au/book/the-carnegie-staged-embryos/id510004473?mt=11 iTunes link
  • iTunes link | iBook Store
  • Description - Imagine the excitement of seeing this incredible early period of human development for the first time. Now consider that much of our initial understanding of human development is based upon study of historic embryo collections. You can now look at these historic images of the first 8 weeks after fertilisation and explore for yourself the changes that occur in human development during this key period. This current book is designed as an atlas of the Carnegie embryo stages with some brief notes and additional information covering the first 8 weeks of development. These images are from from the beginning of last century and are one of the earliest documented series of human embryos collected for basic research and medical education on development. I hope you enjoy learning about the amazing early events that begin to make and shape us. This is the second book in a series of educational releases from UNSW Embryology.
  • Release: First Edition - Mar 12, 2012 ISBN 978-0-7334-3148-7 Print Length 82 Pages, 25.8 MB Language English.
  • PDF Preview version 3.87 MB (Read the associated information, this is an edited educational preview version with many features not functioning).
  • The current website also includes numerous embryo images from this textbook (see Embryonic Development and Carnegie Embryos).

Blechschmidt Collection

7.5mm Embryo movie 1 icon.jpg
 ‎‎Embryo 7.5mm
Page | Play



Image source: The Blechschmidt Collection images are reproduced with the permission of Prof. Christoph Viebahn, director of the Department of Anatomy and Embryology, University of Goettingen. Images are for educational purposes only and cannot be reproduced electronically or in writing without permission.

Madrid Collection

Madrid Collection Embryos  
Carnegie
Stage
Embryo Days CRL (mm) Section
thickness
Staining Section plane
15 GV4 36 7 8 (Stain - Haematoxylin Eosin) transverse

Photograph

Stage15 bf2.jpg Stage15 bf21.jpg

Image: Dr Ed Uthman (Houston, Texas) - other pathology images

Image version links: Virtual Slide | ExtraLarge 1874 x 2000px | Large 959 x 1024px | Medium 468 x 500px

Embryo Virtual Slide

Stage 15 - Ventral View

Stage15 bf2.jpg

 ‎‎Mobile | Desktop | Original

Stage 15 | Embryo Slides

Historic Stage 15 Embryos

  • Carnegie No. 2, 7 mm. Described in detail by Mall (1891).[1]
  • Hochstetter's embryo I, 7 mm. ( (No. 10 of Hochstetter's series) Described in monographic form by Elze (1907).[2]Includes illustrations of reconstructions. Also shown in Fig. 52 of Keibel and Mall (1910).[3]
  • Legg embryo, 7 mm. Described in detail by Thompson (1915).[4]Includes illustrations of reconstructions.
  • 8-mm embryo. The peripheral nervous system was described by Volcher (1963)[5] in this embryo of stage 15.
  • Keibel No. 1495, 8.5 mm. This advanced example of stage 15 was well described in detail by Barniville (1914)[6].
  • Blechschmidt 7.5 mm Blechschmidt Model generated from serial sections of this embryo.
  • Newcastle N340 Optical Projection Tomography of this embryo described by J. Kerwin etal., (2004).[7]

Events

  • Hearing - otic capsule formed by condensed mesenchyme. Ganglion vestibular nerve fibres extend to the otocyst epithelium. External ear auricular hillocks appear, pharyngeal arch 2 forms primordium of the antitragus.
  • Vision - (about 33 days) the lens pit is closed. The lens vesicle and optic cup lie close to the surface ectoderm and appear to press against the surface.[8]
  • Smell - the nasal discs form large oval depressions (nasal pits).
  • Limb - upper limb bud visible as a distal hand plate and a proximal forearm, arm, and shoulder region.
  • Somitogenesis - last stage that somites can be seen from the surface.
  • Cardiovascular
    • Heart - atrioventricular bundle appears, atrioventricular cushions present, conotruncal ridges present, foramen secundum appears and semilunar cusps appear.[9]
    • Coronary circulation plexus of blind epicardial capillaries appears on the heart begin to acquire coronary sinus connection.[10]
    • Cerebral artery vertebral arteries forms from transverse anastomoses between cervical intersegmental arteries, beginning with the proatlantal artery and proceeding downward to the 6th intersegmental artery.[11]
  • Endocrine[12]
    • Epiphysis - pineal body is detectable in the roof of the diencephalon (Stadium I of Turkewitsch 1933) (O'Rahilly 1968).
    • Thyroid - thyroid primordium may be detached from the pharyngeal epithelium in some instances. "At about the time" when the thyroglossal duct "becomes broken it loses its lumen" (Grosser 1912).
    • Adrenal Cortex - primordium is first recognizable. A new type of cell (C1) from the coelomic epithelium is found in the subjacent mesenchyme. New cells (C2) appear in the medial wall of mesonephric glomeruli and begin to migrate into the suprarenal primordium.[13] Jirfisek (1980) denies a mesonephric contribution to the suprarenal.
    • Adrenal Medulla - all types of cells (M1, M2, and M3) increase in number. From stage 15 to stage 18, the suprarenal primordium is cigar-shaped and extends from segment T6 to segment L1, lateral to the aorta and mesogastrium.[13]
  • Meninges (Spinal Cord) - vertebral rudiments now form concentrations that are distinct from the intermediate zone separating them from the neural tube. This intermediary zone, which is less densely cellular and within which lie the ganglia, is the meninx primitiva, from which the meninges have been generally considered to arise. The neural tube is now completely surrounded by endothelium-lined vascular channels. Directly adjacent to the cord, and particularly evident at its ventrolateral surfaces, is a single-layered cell membrane. This is the first indication of the pia mater, and, where it is present, it lies between the vascular channels and the periphery of the spinal cord.[14]

References

  1. Mall FP. A human embryo twenty-six days old. (1891) Journal of Morphology 5: 459-480.
  2. Elze, C. 1907. Beschreibung eines menschlichen Embryo von zirka 7 mm grosster Lange unter besonderer Beriicksichtigung der Frage nach der Enrwickelung der Extremitatenarterien und nach der morphologischen Bedeutung der lateralen Schilddriisenanlage. (Description of a human embryo of about 7 mm greatest length with special reference to the question of the development of the extremities arteries and to the morphological importance of lateral shield primordia). Anat. Hefte, 106, 411-492.
  3. Keibel F. and Mall FP. Manual of Human Embryology I. (1910) J. B. Lippincott Company, Philadelphia.
  4. Thompson P. Description of a human embryo, 7 mm. greatest length. (1915) Studies in Anatomy. University of Birmingham, pp. 1-50.
  5. Volcher, R. 1963. Le systeme nerveux périphérique d'un embryon humain de 8 mm.(The peripheral nervous system of an 8 mm. human embryo) Arch. Biol. (Liege), 74, 95-127. PMID 13997757
  6. Barniville HL. The morphology and histology of a human embryo of 8.5 mm. (1914) J Anat Physiol., 49(1):1-71. PMID 17233012
  7. Janet Kerwin, Mark Scott, James Sharpe, Luis Puelles, Stephen C Robson, Margaret Martínez-de-la-Torre, Jose Luis Ferran, Guangjie Feng, Richard Baldock, Tom Strachan, Duncan Davidson, Susan Lindsay 3 dimensional modelling of early human brain development using optical projection tomography. BMC Neurosci: 2004, 5;27 PubMed 15298700 | PMC514604 | BMC Neurosci.
  8. A A Pearson The development of the eyelids. Part I. External features. J. Anat.: 1980, 130(Pt 1);33-42 PubMed 7364662
  9. L A Arráez-Aybar, A Turrero-Nogués, D G Marantos-Gamarra Embryonic cardiac morphometry in Carnegie stages 15-23, from the Complutense University of Madrid Institute of Embryology Human Embryo Collection. Cells Tissues Organs (Print): 2008, 187(3);211-20 PubMed 18057862
  10. G M Hutchins, A Kessler-Hanna, G W Moore Development of the coronary arteries in the embryonic human heart. Circulation: 1988, 77(6);1250-7 PubMed 3286038
  11. Khaled Menshawi, Jay P Mohr, Jose Gutierrez A Functional Perspective on the Embryology and Anatomy of the Cerebral Blood Supply. J Stroke: 2015, 17(2);144-58 PubMed 26060802 | J Stroke.
  12. O'Rahilly R. The timing and sequence of events in the development of the human endocrine system during the embryonic period proper. (1983) Anat. Embryol., 166: 439-451. PMID 6869855
  13. 13.0 13.1 Crowder RE. The development of the adrenal gland in man, with special reference to origin and ultimate location of cell types and evidence in favor of the "cell migration" theory. (1957) Contrib. Embryol., Carnegie Inst. Wash. 36, 193-210.
  14. Sensenig EC. The early development of the meninges of the spinal cord in human embryos. (1951) Contrib. Embryol., Carnegie Inst. Wash. Publ. 611,

Additional Images

Mall, F. P. 1891. A human embryo twenty-six days old. J. Morph, 5, 459-480.

Carnegie Stages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | About Stages | Timeline



Cite this page: Hill, M.A. 2017 Embryology Carnegie stage 15. Retrieved August 23, 2017, from https://embryology.med.unsw.edu.au/embryology/index.php/Carnegie_stage_15

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© Dr Mark Hill 2017, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G