Carnegie Collection

Embryology - 2 Dec 2023        Expand to Translate
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Introduction

Carnegie Embryos

Measuring embryo size

Carnegie Embryo key card

The Carnegie embryo collection is located at the National Museum of Health and Medicine (NMHM) in Washington, D.C. In addition to the original Carnegie embryos (Carnegie Collection of Embryology), the Human Developmental Anatomy Center holds another 20 historic embryology related collections.

Historically the collection was located at the University of Chicago, then transferred to John Hopkins University with Mall, and then relocated to the Carnegie Institution of Washington. This human embryo collection was begun by Franklin Mall in the early 1900's and many of teh early embryos were listed by him in 1910.

These embryos were also used in many historic papers, in his two volume Manual of Human Embryology textbook, and in the Contributions to Embryology series. For more detailed information about specific Carnegie numbered embryos see Carnegie Embryos.

http://tiny.cc/Carnegie_Collection

Franklin Mall Links: Franklin Mall | 1891 26 Day Human Embryo | 1905 Blood-Vessels of the Brain | 1906 Human Ossification | 1910 Manual of Human Embryology 1 | 1912 Manual of Human Embryology 2 | 1911 Mall Human Embryo Collection | 1912 Heart Development | 1915 Tubal Pregnancy | 1916 Human Magma in Normal and Pathological Development | 1917 Frequency Human Abnormalities | 1917 Human Embryo Cyclopia | 1918 Embryo Age | 1918 Appreciation | 1934 Franklin Mall biography PDF | Mall photograph | Mall painting | Mall painting | Carnegie Stages | Carnegie Embryos | Carnegie Collection | Category:Franklin Mall | Carnegie Embryos | Contributions to Embryology Series

• Department of Embryology Historic Staff
• 

  Franklin P. Mall

• 

  George Corner

• 

  George L. Streeter

• 

  Chester H. Heuser

• 

  Elizabeth M. Ramsey

• 

  Arthur Hertig

• 

  Osborne Heard

• 

  William Discher

Department of Embryology, Carnegie Institution of Washington Historic Staff: Franklin Mall | George Streeter | James Didusch | Chester Heuser | Osborne Heard | Elizabeth M. Ramsey | Arthur Hertig

Carnegie Collection - Carnegie Embryos Sorted by Stage

Carnegie Collection - Stage 3  Serial No. Normality No. of Blastomeres Size of Fixed Specimen (µm) Fixative Embedding Medium Thinness (µm) Stain Year Notes 8663 normal 107 103x80 Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1949 Hertig etal.(1954) 8794 normal 58 108x86 Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1950 Hertig etal.(1954) Abbreviations Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined. Carnegie Collection - Stage 5  Serial No. Stage Grade Fixative Embedding Medium Thinness (µm) Stain Year Notes 8020 5a Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1942 Hertig and Rock (1945a) 8155 5a Exc. Bouin C-P 6 (Stain - Haematoxylin Eosin) 1943 Hertig and Rock (1949) 8225 5a Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1944 Hertig and Rock (1945b) 8004 5b Exc Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1942 Hertig and Rock (1945a) 8171 5b Exc Alc. C-P 6 (Stain - Haematoxylin Eosin) 1943 Hertig and Rock (1949) 8215 5b Exc Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1944 Hertig and Rock (1945c) 9350 5b Exc Bouin ? ? (Stain - Haematoxylin Eosin) 1955 Heuser (1956) 4900 5c Poor p P 10 p 1925 Incomplete. Streeter (1926) 7699 5c Exc. Bouin C-P 6 (Stain - Haematoxylin Eosin) 1939 Hertig and Rock (1941) 7700 5c Exc. Bouin C-P 6 (Stain - Haematoxylin Eosin) 1938 Hertig and Rock (1941) 7771 5c Exc. Bouin C-P 10 (Stain - Haematoxylin Eosin) 1940 Abnormal 7950 5c Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1941 Hertig and Rock (1944) 8000 5c Poor Alc. & Bouin C-P 8 (Stain - Haematoxylin Eosin) 1942 Abnormal 8139 5c Exc. ? C-P 6 (Stain - Haematoxylin Eosin) 1943 Incomplete. Marchetti (1945) 8299 5c Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1945 Abnormal 8329 5c Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1945 Abnormal 8330 5c Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1945 8370 5c Poor Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1946 Abnormal 8558 5c Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1947 Stage 5 was originally subdivided into 3 sequential parts a, b, c. Abbreviations Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined. References Carnegie Collection - Stage 6  Serial No. Grade Fixative Embedding Medium Thinness (µm) Stain Year Notes 6026 Poor ? ? 6? (Stain - Haematoxylin Eosin) 1929 Lockyer embryo. Abnormal. Ramsey (1937) 6734 Poor Zenker—acetic P 10 (Stain - Haematoxylin Eosin) 1934 Yale embryo. Ramsey (1938) 6900 Poor Formol P 15 (Stain - Haematoxylin Eosin) 1940 Linzenmeier (1914) 7634 Poor Formol P 10 (Stain - Haematoxylin Eosin) etc. 1940 Torpin embryo. Kraflca (1941) 7762 Good Zenker—formol P 8 ? 1940 Wilson (1945) 7800 Exc. ? C—P 8 (Stain - Haematoxylin Eosin) 1940 Abnormal 7801 Exc. Bouin C—P 8 (Stain - Haematoxylin Eosin) 1940 Heuser et al. (1945) 7850 Exc. Alc. & Bouin C—P 6 (Stain - Haematoxylin Eosin) 1940 Abnormal 8290 Exc. Bouin C—P 8 H. phlox. 1944 8360 Exc. Alc. & Bouin C—P 6 (Stain - Haematoxylin Eosin), phlox. 1944 8362 Poor p C—P 6 (Stain - Haematoxylin Eosin), phlox 1944 8672 Exc. Ale. & Bouin C—P 6 (Stain - Haematoxylin Eosin) 1949 8819 Exc. Formol—chrom. subl. C—P 8 (Stain - Haematoxylin Eosin) 1951 Edwards-Jones-Brewer (H1496). Brewer (1937, 38) 8905 Poor Ale. p 6 (Stain - Haematoxylin Eosin), phlox. 1951 Abnormal 8910 Good Formol C—P 8 (Stain - Haematoxylin Eosin), phlox. 1951 9222 Good Bouin C—P 6 & 10 Azan 1954 Abnormal. Possibly stage 7 9250 Exc. Bouin p 8 (Stain - Haematoxylin Eosin) 1954 9595 Poor p p 8 (Stain - Haematoxylin Eosin) 1958 10003 Good Bouin p 5 Various 1963 Abbreviations Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined. References Carnegie Collection - Stage 7  Serial No. Grade Fixative Embedding Medium Thinness (µm) Stain Year Notes 7802 Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1940 Heuser et al. (1945) 8206 Good p C-P 6 (Stain - Haematoxylin Eosin) 1943 8361 Good Bouin C-P 10 p 1946 Abnormal 8602 Exc. Alc. C-P 8 (Stain - Haematoxylin Eosin) 1948 8752 Exc. ? C-P 10 (Stain - Haematoxylin Eosin) 1950 8755 Exc. Formol C-P 10 (Stain - Haematoxylin Eosin) 1950 9217 Exc. p P 10 (Stain - Haematoxylin Eosin) 1954 Abbreviations Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined. Carnegie Collection - Stage 8  Serial No. Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes 1399 Poor Formol P Transverse 10 (Stain - Haematoxylin Eosin) etc. 1916 "Mateer embryo" described by Streeter (1920a) 3412 Poor Formol P Transverse 5-15 Al. coch. E. au., or. 1921 5960 Good Kaiserling P Transverse 5 Al. coch. & eosin 1929 Heuser (1932b) 6630 Poor Formol P Oblique 6 (Stain - Haematoxylin Eosin) 1932 6815 Poor Formol P Oblique 10 Al. coch., or. G 1933 7170a and b 7545 Poor Alc. C-P Transverse 6 (Stain - Haematoxylin Eosin) 1935 Twins 7568 Poor Formoi C-P Transverse 6 (Stain - Haematoxylin Eosin) 1938 7640 Good Formol & Bouin P Transverse 10 (Stain - Haematoxylin Eosin) 1939 George (1942) 7666 Exc. Formol-chrom. subl. C-P Transverse 6 (Stain - Haematoxylin Eosin) 1939 "H. 1515" 7701 Exc. ? C-P Transverse 8 (Stain - Haematoxylin Eosin) 1939 7822 Good Formoi C-P Transverse 10 (Stain - Haematoxylin Eosin) 1940 7949 Good Zenker p Sagittal 10 (Stain - Haematoxylin Eosin) etc. 1941 7972 Good Alc. & Bouin C-P Sagittal 6 (Stain - Haematoxylin Eosin) 1942 8255 Exc. Bouin C-P Sagittal 8 (Stain - Haematoxylin Eosin), phlox. 1944 Slides showing embryo returned to Dr. Patten in 1962 8320 Good Formol C-P Sagittal 8 (Stain - Haematoxylin Eosin), phlox. 1945 8352 Good Formol C-P Transverse 8 (Stain - Haematoxylin Eosin), phlox. 1946 8371 Poor Alc. & Bouin C-P Sagittal 8 (Stain - Haematoxylin Eosin), phlox. 1946 8671 Exc. Alc. & Bouin C-P Sagittal 6 (Stain - Haematoxylin Eosin), phlox. 1949 8725 Exc. Alc. & Bouin C-P Sagittal 6 (Stain - Haematoxylin Eosin), phlox. 1949 Preparation method described by Heard (1957) 8727 Exc. Alc. & Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin), phlox. 1949 Germ disc folded, possibly double (Hertig, 1968, fig. 180) 8820 Good Zenker-formol ? Transverse 10 (Stain - Haematoxylin Eosin) 1951 "Jones-Brewer I" (H. 1459) described by Jones and Brewer (1941) 9009a and b 9123 Good Formol C-P Sagittal 6 (Stain - Haematoxylin Eosin) 1952 Twins described briefly by Heuser (1954) 8371 Good Formol C-P Sagittal 6 (Stain - Haematoxylin Eosin) 1953 9251 Good ? C-P Sagittal 10-12 Azan, H. & phlox. 1954 9286 Exc. Formol C-P Transverse 8 Azan 1955 10157 Exc. Formol C-P Transverse ? Cason 1967 10174 Exc. Bouin p Transverse 8 Cason 1967 Abbreviations Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined. References Carnegie Collection - Stage 9  Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes 1878 2-3 Embryo, 1.38 Ch., 12x10.5x7.5 Good Formalin P Coronal 10 (Stain - Haematoxylin Eosin) 1917 Described by Ingalls (1920). 5080 1 Embryo, 1.5 Ch., 14.5 Poor Formalin P Transverse 10 Al. coch. 1926 Studied by Davis (1927). 7650 2-3 Embryo, 2-3 Good Alc & Bouin C-P Transverse 6 (Stain - Haematoxylin Eosin) 1939 Said to be female Abbreviations Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined. References Carnegie Collection - Stage 10  Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Thinness (µm) Stain Year Notes 391 8 E, 2 Ch., 14 Good Formalin P 10 Al. coch. 1907 Monograph by Dandy (1910) 1201 7 E,2 Ch.. 144 Good Formalin P 8 H. & or. G. 1915 Univ. Chicago No. H 87 2795 4-5 E,2 Poor Alc. P 6 Al coch,or.G. 1919 3707 12 E, 1 5 Good Formalin P 12.5 I. H. 1921 Univ. Calif. No. H 197 3709 4 E. 1.4 Ch.. 14.8 Poor Formalin P 10 Erythrosin 1921 Univ. Chicago No H 279 3710 12 E., 3.6 Ch., 19.0 Good Formalin C-P 10 H. & or. G. 1921 Univ. Chicago No. H 392 4216 8 E, 2 Ch, 9.8 Good Formalin P 15 ? 1923 Monograph by Payne (1925) 5074 10 E., 3.3 Ch., 10.8 Exc. Bouin P 10 Al. coch. 1925 Univ. Rochester No. H 10. Monograph by Corner (1929) 6330 7 E, 2.83 Good P 5 Ehr. H. 1931 Univ. Chicago No. H 1404 6740 12 E., 2.2 Good p C-P 8 ? 1933 Litzenberg embryo. Studied by Boyden (1940) 7251 8 E., 1.27 Good Formalin C-P 10 (Stain - Haematoxylin Eosin) 1941 "Singapore embryo." Univ. Cambridge No. H 98. Studied by Wilson (1914) 8244 6 E., 1.55 Ch, 8,5 Good Alc. C-P 8 (Stain - Haematoxylin Eosin) phlox. 1944 9870 12 Ch, ca. 8 Good Zenker P 5 Various, chiefly carmine 1952 Univ. Chicago. No. H 637. Dicephaly Abbreviations Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined. All Carnegie stage 10 embryos are cut in Transverse plane. Carnegie Collection - Stage 11  Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes 12 14 E, 2.1 Ch, 13 Poor P Transverse 10 Al. carm. 1893 164 18 E, 3.5 Ch, 14 Good Formalin P Transverse 20 Al. carm. 1913 318 13/14 E, 2.5 Ch, 16 Good P Transverse 25 Al. carm. 1905 470 17 E, 4.3 Ch, 16 Good Formalin P Transverse 10 Al. carm. . 1910 779 14 E, 2.75 Good C Transverse 15 Al. coch. 1913 Dysraphism. Noted by Dekaban (1964) 1182b E, 3 Ch, 15x12x5 Good Formalin ? Transverse 20 Al. carm. 1915 2053 20 E, 3.1 Ch, 12 Exc. Formalin P Transverse 10 Al. coch. 1918 Most advanced in group. Ag added to slide 2 Monographs by Davis (1923) and Congdon (1922) 4315 17 E, 4.7 Ch, 23x10.4X11 Excellent ? C-P Transverse 10 I.H. & E. 1923 Univ. Chicago No. 951. Wen (1928) 4529 14 E, 2.4 Ch, 21 Excellent Formalin P Transverse 10 Al. coch, or. G. 1924 Heuser (1930) 4783 13 E, 2.3 Fair ? ? Transverse 5 I.H. 1924 Wallin (1913) 4877 13 E, 2 Ch, 15 Good Formalin P Transverse 15 Al. coch. 1925 5072 17 E, 3 Good Formalin P Transverse 10 (Stain - Haematoxylin Eosin) 1925 Tubal Type specimen. Atwell (1930) 6050 19/21 E.,3 Ch, 10 Good Formalin C-P Coronal 10 Al. coch. 1930 Advanced 6344 13 E, 2.5 Ch, 17 Excellent Formalin C-P Transverse 6 Al. coch. 1931 Least advanced in group 6784 17 E, 5 Ch, 16 Excellent Formalin C-P Transverse 6 I.H, or. G. 1933 7358 16 E, ? Ch, 15 Poor Alc, formol p Oblique 25 (Stain - Haematoxylin Eosin) 1936 7611 16 E., 2.4 Ch., 12 Excellent Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1938 7665 19 E., 4.36 Excellent ? C-P Transverse 6 1939 Univ. Chicago No. H 1516 7702 17 E, 3.7 Ch., 14 Good Formalin C-P Transverse 10 Al. coch. 1940 Returned to B M Patten 7851 13 E., 4.3 Ch, 18 Excellent Formalin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1940 Slightly injured 8005 16/17 E, 3 Excellent Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1942 Tubal 8116 17 E, 14 Ch.. 17 Good Formalin p Sagittal 8 Azan 1953 8962 15 E, 1.55 Good ? * Sagittal ? ? 1952 Tubal Univ. Chicago No. H 810 Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined. References Carnegie Collection Embryos - Stage 12  Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes 209 ca_24 EH3 Ch.,15 Poor Alc P Coronal 50 Al. coch. 1902 250 19? E , 2 (11, 10x9x9 Poor p ? Sagittal 20 Al. coch. p 384 P E 2_5 Ch.,13 Poor Formalin P Transverse 10 H.&E. 1907 Macerated. Narrow yolk stalk 486 21 E.,4 Ch., 22 Good Corros. acetic P Transverse 10 Al. Coch. 1911 1062 29 E.,4.5 Ch., 20 Good Formalin P Transverse 20 Al. coch. 1915 Transitional to next stage 2197 ? E_,5_3 Ch., 19.5 Poor Formalin P Transverse 10 Al. coch, or. G. 1918 4245-7 ca.24 E.,3.5 Ch., 24 Good Alc, formol P Transverse 10 Al. coch. 1923 Caudal neuropore widely open 4479 P E.,5 .8 Ch , 17 Poor Formalin P Transverse 40 Al. coch. 1923 Macerated. Upper lirnb buds not visible 4736 26 E.,3.0 Ch.,20 Good Formalin P Coronal 10 Al. coch. 1924 No upper limb buds. Caudal neuropore closed 4759 ? E.,4.5 Ch.,15 Good Formalin P Transverse 15 (Stain - Haematoxylin Eosin) 1924 Neural tube folded 4784 23 E_,3 Good P P Transverse 10 p 1924 5035 25-28 E.,3.8 Ch.,18 Good Formalin C-P Transverse 10 Al. coch. 1925 5048 ca_25 E_,3_5 Good Formalin C-P Transverse 10 Al. coch. 1925 Tubal Injured 5056 25 E,,3 Ch.,12 Good Formalin P Transverse 10 Al. coch. 1925 5206 ? E.,4 Ch., 51x31x30 Poor ?? P Transverse 20 Al. coch. 1926 Tubal 5300 ? E., 4.5 Ch,16.5 Poor Formalin P Transverse 20 Al. coch. 1926 Autopsy. Partly macerated 5923 28 E.,4 Ch.,15 Exc. Formalin P Transverse 10 Al. coch. 1929 6097 25 E,3.4 Ch., 12.5 Exc. Formalin C-P Transverse 10 Al. coch, eosin 1930 Tubal Ag added to slides 1-3 6144 27 E. 3.3 Ch.,11 Good Lysol—Zenker C—P Transverse 10 Al. coch. 1930 6488 28 E, 32 Ch,22 Good Formalin C—P Transverse 10 Al. coch. 1932 6937 26 E.,3 Ch , 12 Poor Formalin C—P Coronal 10 I.H.,or.G. 1934 Tubal Caudal neuropore closed 7724 ca.29 E,3.5 Ch.,18 Good Formalin C—P Sagittal 8 (Stain - Haematoxylin Eosin) 1940 Caudal end broken 7852 25 E , 3.7 Ch,26 Exc. Formalin C—P Transverse 10 (Stain - Haematoxylin Eosin) 1940 Typical for stage 12 7999 ca.28 E,3.2 Ch , 15 Exc. Bouin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1942 Caudal defect 8505a 24 Ch, 23.5 Exc. Formalin C-P Transverse P H. Phlox. 1947 8505b 23 Ch,24 Exc. Formalin C-P Sagittal p Azan 1947 Twins 8941 28 E,4.9 Ch, 35 Exc. Zenker C-P Transverse 6 I.H. 1927 Univ. Chicago No. H 1261 8942 25 E, 38 Ch, 35 Exc. Formal-Zenker C-P Coronal 5 11-1. 1930 Univ. Chicago No. H 1382 8943 22 E. 3.9 Ch, 20.4 Exc. Formal-Zenker C-P Transverse 8 H.&E. 1934 Univ. Chicago No. H 1481 8944 25 E,4 Ch,,25 Exc. Formal-Zenker C-P Sagittal 8 I.H. 1936 Univ. Chicago No. H 1514 8963 22 E, 3.8 Ch , 14.5 Fair Formalin C-P Transverse 10 I.H. 1928 Univ. Chicago. No. H 1093 Studied by Wen (1928) 8964 23 E,2.8 Ch - 25 Poor Formalin p Transverse 8 I.H. 1928 Univ_ Chicago No. H984 Studied by Wen (1928) 9154 24 E, 5 4 Exc Formalin C-P Transverse I.H. & phlox. 1953 Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined. References Carnegie Collection Embryos - Stage 13  Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes 1 E.,4.5 Ch., 30x30 Poor Salicylic acid P Transverse 10 Hemat. 1887 Obtained by Mall while student 19 E., 5.5 Ch., 18x14 Poor p ? Transverse 20 Al. coch. 1895 98 E., 4 Ch., 24x16x9 Poor p P Transverse 20 Al. coch. 1896 76 E., 4.5 Ch., 22x20 Poor Alc. P Transverse 20 Al. coch. 1897 112 E., 4 Poor p P Sagittal 10 Al. coch. p 116 E., 5 Poor p ? Sagittal 10 Al. coch. 1898 148 E.,4.3 Ch., 17x14x10 Poor Alc. P Coronal 10 (Stain - Haematoxylin Eosin) 1899 Abnormal. Nasal discs fused 186 E.,3.5 Ch., 25x20x15 Poor Alc. P Transverse 20 Al. coch. 1901 239 E., 3.0 Poor Formalin P Transverse 10 (Stain - Haematoxylin Eosin) 1903 248 E., 4.5 Ch., 30x23x15 Poor p ? Coronal 50 Al. coch. 1904 407 E.,4 Ch., 14x13X7 Poor Formalin ? Transverse 40 Al. coch. 1907 463 E., 3.9 Ch., 17x12x7 Good Formalin P Coronal 10 Al. coch. 1910 523 E., 5 Ch., 25x25x15 Fair Formalin P Transverse p Al. coch. 1911 588 E., 4.0 Ch., 19x15x8 Good Corros. acetic P Coronal 15 (Stain - Haematoxylin Eosin) 1912 Advanced 786 E., 4.5 Ch., 19x10x10 Poor Alc. P Sagittal 15 Al. coch. 1913 800 E., 6.0 Good Corros. acetic P Transverse 10 H 1913 Curettage. Anencephaly 808 E.,4.0 Poor Corros. acetic P Transverse 15 Al. coch. 1914 Tubal Incomplete 826 E., 5.0 Ch., 13x13x9 Good Formalin P Transverse 20 Al. coch. 1914 Shrunken 836 E.,4.0 Ch., 22x18x11 Exc. Corros. acetic P Transverse 15 Al. coch. 1914 Less advanced 963 E.,4.0 Ch., 23x18x16 Good Formalin P Coronal 20 Al. coch. 1914 1075 E.,6.0 Ch., 46x32x20 Exc. Corros. acetic P Coronal 20 (Stain - Haematoxylin Eosin) or. G. 1915 Most advanced in group 3956 E., 4.0 Poor Formalin P Transverse 20 Al. coch. 1922 Tubal Incomplete 4046 E.,5 Ch., 22x20x20 Poor Formalin P Transverse 50 Al. coch. 1922 5541 E., 6.0 Ch., 35x30x20 Good Formalin P Transverse 10 Al. coch., eosin 1927 5682 E., 5.3 Ch., 29x25x13 Poor Formalin P Coronal 20 Al. coch. 1928 5874 E., 4.8 Exc. Bouin P Transverse 10 (Stain - Haematoxylin Eosin) 1929 Hysterotomy. Bromides only 6032 E., 5.8 Ch., 30x24x13 Poor Formalin P P ? p 1929 Not good enough to cut 6469 E., 5.0 Ch., 25x18x18 Poor Formalin P P P P 1932 Fragmented on cutting. Not saved 6473 E., 5.0 Ch, 30x30x15 Exc. Formalin C-P Coronal 6 Al. coch. 1932 Less advanced. Ag added 7433 E., 5.2 Ch., 15x13x13 Exc. Formalin C-P Coronal 8 (Stain - Haematoxylin Eosin) 1937 Tubal 7618 E, 48 Ch, 18x15x15 Exc. Bouin C-P Coronal 10 (Stain - Haematoxylin Eosin) 1939 Hystereaomy. Advanced. Ag added 7669 E, 5.0 Ch., 23x16x14 Good Formalin C-P Coronal 6 (Stain - Haematoxylin Eosin) 1939 Hysterectomy. Least advanced in group, Ag added 7889 E, 4.2 Exc. Bouin C-P Coronal 6 (Stain - Haematoxylin Eosin) 1941 Hysterectomy 8066 E,53 Ch , 20x18xI8 Exc. Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1942 Hysterectomy. Ag added to slide 2 8119 E., 5.3 Ch., 32x28x6.5 Exc. Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1943 Hysterectomy 8147 E., 5.2 Ch., 27x21x19 Poor Formalin ? ? p p 1943 Tubal Not cut 8239 E., 4.3 Exc. Bouin C-P Sagittal 8 H. phlox. 1944 8372 E., 5.6 Exc. Alc.& Bouin P Transverse 10 Azan 1946 8581 E., 4.8 Good Kaiserling C-P Sagittal 8 Azan 1948 Most-advanced third 8967 E., 5.7 Exc. Acetic Zenker C-P Transverse 6 (Stain - Haematoxylin Eosin) 1931 Head injured. Univ. Chicago No. H1426 9296 E,4.5 Exc. C-P Coronal 8 Azan 1955 9297 E., 4.5 Exc. C-P Sagittal 8 Azan 1955 9697 E., 5.5 Bouin 1956 not cut Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined. Carnegie Collection Embryos - Stage 14  Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes 4 E.,7 Poor p P Transverse 10 Al. coch. 1892 18 E.,7 Ch., 18x18 Poor p P Transverse 20 Al. coch. 1895 80 E., 5.0 Ch., 24x18x8 Good Alc. P Transverse 20 Al. coch. 1897 187 E.,7 Ch., 35x30x25 Poor ? P Sagittal 20 Al. coch. 1902 1208 E.,7 Ch., 22x11Xx1 Poor ? P Sagittal 20 Al. coch. 1902 245 E.,6 Ch., 13x12x10 Poor Formol, Zenker ? Transverse 5 (Stain - Haematoxylin Eosin) 1904 372 E.,7 Fair p P Transverse 10 H.-Congo red 1902 380 E,6 Ch., 20x20x14 Poor p P Sagittal 20 (Stain - Haematoxylin Eosin) 1906 387 E.,7 Ch., 45x40x50 Good Formalin P Transverse 20 (Stain - Haematoxylin Eosin) 1907 442 E.,6 Ch., 25x20 Poor Formalin P Coronal? 50 Al. coch. 1908 552 E.,6 Ch, 40x28Xx8 Poor p P Sagittal 40 Al. coch. 1911 Possible anencephaly 560 E., 7.0 Ch, 24x24 Poor Formalin P Coronal 40 Al. coch. 1912 676 E., 6.0 Ch, 35x20x17 Good Carnoy P Tr.-Coronal 20 (Stain - Haematoxylin Eosin) 1913 Possible spina bifida 873 E,6.0 Ch., 35x28x16 Poor Formalin P Sagittal 20 Al. coch. 1914 988 E,6.0 Ch., 38x30x23 Good Formol-corros. acetic P Transverse 20 Al. coch. 1914 1380 E , 5.7 Ch, 36x24x24 Exc. Formalin P Coronal 20 Al. coch. 1916 1620 E, 6.6 Ch, 35x30x8 Good Formalin P Sagittal 20 Al. coch. 1916 ?? E, 6.68 Fair ? ? Transverse 6 Al. coch., or. G 1919 2841 E , sy Ch. 35x21 Good Alc. P Transverse 20 (Stain - Haematoxylin Eosin) or.G 1920 3360 E.6.0 Good Formalin C Transverse 20 (Stain - Haematoxylin Eosin) or.G 1920 In myomatous uterus. Advanced. 3805 E., 5.9 Exc. Bouin P Transverse 15 (Stain - Haematoxylin Eosin) 1921 Evans embryo No. 168. Serial bromides only 3960 E., 5.5 Good Formalin C-P Coronal 20 Al. coch. 1922 Blood vessels naturally injected 4154 E, 6.8 Ch., 33x31x20 Poor Alc. C-P Transverse 8 (Stain - Haematoxylin Eosin) 1923 4245-6 E., 7.0 Good Formalin P Transverse 15 Al. coch. 1923 Univ. Pennsylvania No. 40. Ag added 4692 E., 6.5 Ch., 32x23 Good Formalin C-P Sagittal 10 (Stain - Haematoxylin Eosin) 1924 4672 E,8.2 Ch., 40X34X25 Good Formalin P Transverse 20 Al. coch. 1924 Advanced 4805 E., 7.3 Ch., 15X8X9 Good Formalin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1924 Tubal 5437 E., 7.0 Good Formalin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1927 Advanced 5654 E., 5.0 Ch., 30x23x17 Good Formalin P Transverse 10 Al. coch., eosin 1928 Less advanced 5787 E., 6.8 Ch., 32x30x23 Good Formalin P Sagittal 10 Al. coch., eosin 1928 6428 E., 7.0 Ch., 30X28X25 Good Formalin C-P Coronal 6, 10 Al. coch. 1931 Advanced 6500 E, 4.9* Good Souza? C-P Sagittal 10 Al. coch. 1931 E. Leitz, Berlin 6502 E., 6.7* Exc. Souza? C-P Transverse 5, 10 (Stain - Haematoxylin Eosin) 1931 E. Leitz, Berlin. Ag added to slides 1-25 6503 E., 6.3* Exc. Souza? C-P Coronal 10 Al. coch. 1931 E. Leitz, Berlin 6739 E.,8 Poor Formalin C-P Sagittal 20 (Stain - Haematoxylin Eosin) 1933 6830 E,5.5 Ch., 47x23x15 Exc. Formalin C-P Coronal 8 (Stain - Haematoxylin Eosin) 1933 6848 E., 7.8 Good Formalin C-P Coronal 10 (Stain - Haematoxylin Eosin) 1934 Tubal 7324 E, 6.6 Ch., 17x13x10 Good Formalin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1936 Low implantation 7333 E, 6.3 Good Formalin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1936 7394 E, 7.2 Ch., 45x20x20 Exc. Formalin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1937 7400 E, 6.3 Ch., 35x25x20 Good Formalin C-P Coronal 10 (Stain - Haematoxylin Eosin) 1937 7522 E., 7.7 Ch., 33x16x16 Good Formalin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1938 Natural blood injection 7598 E., 7.0 Ch., 30x30x25 Poor Alc. C-P Transverse 10 (Stain - Haematoxylin Eosin) 1938 Macerated 7667 E., 5 Ch., 16x14x12 Fair Formalin P Transverse 8 (Stain - Haematoxylin Eosin), phlox. 1939 7829 E., 7.0 Exc. Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1940 Advanced 7870 E,7.2 Ch., 25x20x13 Exc. Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1941 On borderline of next stage. Ag added 8141 E,7.3 Ch.,33x28 Exc. C-P Coronal 8 (Stain - Haematoxylin Eosin) 1943 Shrinkage cracks in brain 8306 E.5.3 Ch., 27 Exc. Bouin C-P Transverse 10, 20 (Stain - Haematoxylin Eosin), phlox. 1945 8308 E, 5.85 Ch., 27x18x18 Exc. Formol & Bouin C-P Sagittal 8 Azan 1945 8314 E,8 Ch.,23x22 Exc. Formol C-P Transverse 8 Azan 1945 8357 E., 6.5 Good Formol C-P Sagittal 8 Azan 1946 8552 E,6.5 Exc. Alc. & Bouin C-P Transverse 8 Azan 1947 8999 E,6 Ch.,16x15 Exc. Alc. & Bouin C-P Sagittal 8 Azan 1952 9695 E,8.5 ? 1955 Not cut Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined. Carnegie Collection Embryos - Stage 15  Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes 2 E., 7.0 Ch., 25x25x25 Good Alc. P Transverse 15 Al. carm. 1888 Least-advanced third 88 E.,8 Ch., 30x28x15 Poor Alc. P Coronal P Al. coch. 1897 113 E.,8 241 E,6.0 Poor P p Sagittal 10 Borax carmine ? 241 E,6.0 Good Formalin P Transverse 10 H. & Congo red 1904 371 E,6.6 Good Formalin P Sagittal 10 Al. coch. 1913 Shrunken and cracked 389 E., 9 Poor p p Sagittal 20 (Stain - Haematoxylin Eosin) 1907 Tubal 721 E., 9.0 Ch., 30x20x10 Exc. Zenker formol P Transverse 15 (Stain - Haematoxylin Eosin) 1913 Median in group 810 E., 7.0 Ch, 30x25x15 Good Alc. P Sagittal 20 Al. coch 1913 855 E.,7.5 Poor Formalin P Transverse 100 Al. coch. 1914 Pathological between limbs 1006 E,9.0 Ch., 37x26x22 Poor Formalin P Coronal 20 (Stain - Haematoxylin Eosin) or. G. 1914 Operative. Most-advanced third 1091 E,7.2 Ch., 28x26x20 Poor P P Coronal 20 Al. coch. 1915 Macerated 1354 E,7.8 Ch, 35x30x25 Good Formalin P Sagittal 20 Al. coch. 1916 Least-advanced third 1767 E , 11.0 Ch, 41x23x5 Good Formalin P Sagittal 40 (Stain - Haematoxylin Eosin) or. G. 1917 Most-advanced third 2743 E., 7.2 Ch., 19xl8x14 Poor Formalin P Transverse 20 Al. coch. 1919 Macerated. Least-advanced third 3216 E, 6.5 Ch, 30x30x5 Good Formalin P Transverse 20 Al. coch. 1920 Hysterectomy. Least-advanced third 3385 E,83 Ch., 25x20x16 Exc. Corros. acetic P Trans. 20 (Stain - Haematoxylin Eosin) or. G. 1921 Some sections lost. Most-advanced third. Ag added 3441 E,8.0 Ch., 25x24x20 Good Formalin P Sag. 10 Al. coch. 1921 3512 E,8,5 Ch., 33x28x25 Good Formalin P Trans. 10 Al. coch. 1921 3952 E,6,7 Ch., 30x25x15 Good Formalin P Cor. 15 Al. coch. 1922 Median in group 4602 E,9.3 Ch,, 33x30x26 Good Formalin P Sag. 15 Al. coch. 1924 Medical abortion 4782 E,9.0 Ch., I4xl3x11 Poor Formalin P Cor. 20 Al. coch. 1924 5772 E, 8 Poor ? P Cor. 15 Al. coch. eosin 1928 Template:CE5?92 E, 3 Good Corros. acetic C-P Cor. 10 Al. coch. phlox 1929 Transitional to next stage 6223 E ? Poor Alc. C-P Sag. 8 Or. G. 1930 Fragmented sections. Not saved 6504 6506 6508 6595 ???? Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined. Carnegie Collection Embryos - Stage 16  Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes 163 E., 9.0 Ch., 35x35x20 Good Formol P Transverse 20 Al. coch. 1899 Used by Bardeen and Lewis 221 E., 7.5 Ch., 40x33x33 Poor Formalin P Sagittal 20 Al. coch. 1903 Macerated 383 E., 7.0 Ch., 15x15x15 Poor Formalin P Transverse 10 Al. carm., H. & Congo red 1904 397 E., 8.0 Ch., 15x15x15 Poor Formalin P Transverse 10 (Stain - Haematoxylin Eosin) 1907 422 E., 9.0 Ch., 30x30x30 Poor Alc. P Transverse 40 Al. coch. 1910 Tubal. Partly macerated 559 E., 8.6 Ch., 20x15x12 Good Formalin P Transverse 20 H. & Congo red 1911 Cyclopia. Formerly listed as stage 17 589 E., 11 Ch., 30x13x13 Poor p P Sagittal 50 Al. coch. 1912 617 E., 7.0 Ch., 18x14x12 Good Formalin P Transverse 15 Al. coch. 1912 Median in group 636 E., 10 Ch.,28x28x22 Poor Formalin P Transverse 50 Al. coch. 1913 Macerated 651f E., 7 Ch., 25x20x15 Poor p p p p p 1913 Spina bifida 675 E., 10 Ch., 50x30x25 Poor Formalin P Sagittal 100 Carmine 1915 Abnormal head and limbs 792 E., 8.0 Ch., 40x30x30 Good Formalin P Transverse 20 Al. coch. 1913 Advanced 887 E, 9.0 Ch., 31x28x17 Good Formalin P Transverse 40 Al. coch. 1914 Near next stage 1121 E., 11.8 Good Corros. acetic P Coronal 40 Al. coch. 1915 Operative. Median in group 1197 E., 10.0 Ch., 23x19x15 Good Formalin C Sagittal 20 (Stain - Haematoxylin Eosin), or. G. 1915 Advanced 1544 E., 7.2 Good Zenker P Sagittal 20 Al. coch. 1916 Tubal. Mechanical injury 1836 E., 11.0 Good Formalin P Transverse 20 (Stain - Haematoxylin Eosin) 1917 Most-advanced third 4677 E., 9.5 Ch., 48x36x30 Good Formalin P Transverse 10 Al. coch. 1924 Median in group 5515 E., 12.0 Ch., 47x37x25 Good Formalin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1927 Near next stage 6054 E., 7,0 Ch., 21x17x12 Good Formalin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1930 Least-advanced third 6507 E., 9.0* Excellent Corros. acetic C-P Coronal 10 & 8 Al. coch. p Middle or most-advanced third 6509 E. 8.1* Excellent Corros. acetic C-P Coronal 10 Al. coch. p Least-adianced or middle third 6510 E., 10.1* Excellent Corros. acetic C-P Coronal 10 Al. coch. p Close to No. 6507. Ag added 6511 E, 8.1* Good Corros. acetic C-P Sagittal 10 Al. coch., iron H. p Surface injured by fixative. Most-advanced third 6512 E., 7.0* Excellent Corros. acetic C-P Transverse 10 Al. coch. p Least-advanced third. Borderline 6513 E., 7.2* Good Corros. acetic C-P Coronal 10 Al. coch. p Surface injured by fixative. Least advanced in group 6514 E, 9 0* Poor Corros. acetic C-P Sagittal 10 Al. coch. p Most-advanced third 6516 E., 10 5* Good Corros acetic C-P Sagittal 8 Al. coch. p Most-advanced third. Double left kidney and ureter 6517 E., 10.5* Excellent Corros. acetic C-P Transverse 8 Al. coch. ? Close to No. 6516 6686 E., 11.0 Ch., l7x17xP Poor Formalin C-P Coronal 20 Al. coch. 1933 Tubal. Partly macerated 6750 E., 10.0 Good Formalin C-P Transverse 10 H. & phlox. 1933 Tubal. Advanced 6909 E., 11.0 Good Bouin C-P Coronal 10 (Stain - Haematoxylin Eosin) 1934 Tubal. Advanced 6931 E., 8.8 Ch., 3""x 33x16 Good Formalin C-P Coronal 10 Al coch., phlox. 1934 Least-advanced third. Type specimen 6950 E.. 9 0 Ch., 3lx20x18 Good Formalin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1934 Tubal. Partly fragmented 7?15 E., 9.7 Exc Bouin C-P Coronal 10 H. & phlox 1935 Operative. Less advanced 7629 E., 11.5 Ch., 31x31 Good Formalin C-P Coronal 10 Al. coch., phlox 1939 Hysterectomy. Most advanced in group 7804 E., 9.5 Ch., 26x21x16 Good Formalin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1940 Least-advanced third 7897 E., 12.2 Ch., 31x24x23 Good Formalin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1941 Tubal. Advanced 8098 E., 10.0 Ch., 30 Good Formalin C-P Coronal 6 (Stain - Haematoxylin Eosin) 1942 Tubal. Median in group 8112 E., 10.9 Excellent Bouin C-P Coronal 8 (Stain - Haematoxylin Eosin) 1943 Most-advanced third 8179 E., 11.9 Ch., 23x18x17 Good Formalin C-P Coronal 10 (Stain - Haematoxylin Eosin) 1943 Tubal 8436 E., 10.9 Ch., 13x15x1? Good Formalin P Coronal 10 Azan 1946 Advanced 8692 E., 10 Good Bouin P Transverse 10 (Stain - Haematoxylin Eosin) 1949 Rubella. Medical abortion. Mechanically damaged 8697 E., 11.3 Poor Formalin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1949 Perhaps stage 17 8773 E, 11 Excellent Bouin P Coronal 10 Azan 1950 8971 E., 10 Ch., 20.5x14.5x13.7 Poor Formalin Transverse 15 (Stain - Haematoxylin Eosin) 1932 Synophthalmia. Univ. Chicago No. H 1439 Template:CE9055 E., ca. 10 Excellent Bouin P Transverse 20 Azan & Ag 1953 Damaged 9229 E, 9.5 Excellent Formalin P Transverse 6 Ag & (Stain - Haematoxylin Eosin) 1954 Stage 15, 16, or 17? Mislaid Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin Bouin solution) Stain - ? - unknown or not determined. Carnegie Collection Embryos - Stage 17  Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes 353 E, 11.0 Ch., 40x35x20 Good Formalin P Coronal 10 (Stain - Haematoxylin Eosin) 1906 Very advanced 485 E, 13.0 Ch., 33x25 Exc. Formalin P Coronal 40 Al. coch. 1911 Injected (India ink) 544 E., 11.5 Ch., 30 Good Zenker-Formol P Sagittal 40 Al. coch. 1911 Operative Injected (India ink) 562 E, 13.0 Ch., 28x17x17 Poor Formalin P Sagittal 100 Al. coch. 1912 Advanced 623 E 10.1 Good Alc. P Transverse 20 H. & Congo red 1912 Operative. Median in group 695 E, 13.5 Ch., 40x40x17 Poor Formalin P Transverse 10 H. & Congo red 1913 Macerated 916 E, 11.0 Ch, 30x30x16 Good Bouin C Transverse 40 H.&E, or. G. 1915 Most-advanced third 940 E, 14.0 Ch, 28x23x21 Good Formalin C Transverse 40 H.&E, or. G. 1914 Advanced 1232 E, 14.5 Ch., 35x35x30 Poor Formalin P Coronal 40 Al. coch. 1915 Close to No.1267A 1267A E-a 145 Ch., 35x30x26 40 Good Formalin C Sagittal 20 (Stain - Haematoxylin Eosin). or. G 1915 Excellent CN.S. 1267B ?? E, 125 Good Formalin p Sagittal 20 (Stain - Haematoxylin Eosin) 1917 Tubal 5642 E, 11.5 Ch.. 33x30x17 Good Formalin p Transverse 15 Al. coch. 1928 Right upper limb injured 5893 E.. 13.2 Good Formalin C-P Transverse 20 Al. coch. 1929 Most advanced in group 6258 E. 14.0 Ch 48x35x25 Good Formalin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1930 Median in group 6519 E.. 10.8* Exc Corrov acetic C-P Sagittal 8 Al. coch. ? Least-advanced or middle third 6520 E., 14.2* Exc. Corros. acetic C-P Transverse 10 Al. coch. P Median in group. Ag added to slides 1-25 6521 E., 13.2* Exc. Corros. acetic C-P Transverse 8-18 Al. coch. ? Sections vary in thinness 6631 E., 13.0 Good Formalin C-P Coronal 10 (Stain - Haematoxylin Eosin) 1932 Tubal. Advanced 6742 E, 11.0 Ch.,50x40x15 Good Formalin C-P Transverse 12 H. & phlox. 1933 Good primary germ cells 6758 E., 12.8 Good Formalin C-P Transverse 10 H. & phlox. 1933 Least—advanced third 7317 E, 10.0* Good P Coronal 10 (Stain - Haematoxylin Eosin) 1936 His embryo “Ru." Every third section 7436 E., 13.0 Good Formalin C-P Coronal 30 Al. coch. 1937 Most-advanced third 8101 E., 13.0 Exc. Bouin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1943 Operative 8118 E., 12.6 Exc. Bouin C-P Coronal 10 (Stain - Haematoxylin Eosin) 1943 Middle third 8253 E., 11.2 Ch.,30x20x10 Good Bouin C-P Coronal 10 Al. coch., phlox. 1944 Operative. Least advanced in group 8789 E., 11.7 Exc. Bouin C-P Sagittal 10 Azan 1950 8969 E., 11.2 Exc. ? p Transverse 15 Azan 1919 Univ. Chicago No. H566 8998 E., 11.0 Exc. ? C-P Coronal 10 Azan 1952 9100 E., 12.0 Ch., 12x13x10 Exc. Formol-chrom. subl. C-P Sagittal 10 Azan 1933 Univ. Chicago No. H1475 9282 E, 12.0 Ch., 16 Good Ale. p Transverse 15 Ag 1955 Mislaid Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined. Carnegie Collection Embryos - Stage 18  Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Semi. ducts P.-M. duct (mm) Year Notes 109 E., 12.0* Ch.,30 Poor Alc. P Transverse 20 Al. coch. 1 0.4 1897 Tubal Least—advanced third 144 E., 16.0* Ch, 40x30x30 Good Formalin P Sagittal 40 Al. eoch. 3 0.85 1899 Most—advanced third 175 E., 13.0 Ch, 30x25x25 Poor Alc. P Transverse 20 Al. coch. 2 0.6 1900 Tubal Partly macerated 296 E., 17.0 Poor Ale. P Coronal 20 Various 3 0.85 1905 Most—advanced third 317 E., 16.0 Good Formalin P Coronal 20 (Stain - Haematoxylin Eosin) or. G. 2 0.7 1905 Middle third 351 E.,14.0* Good Formalin P Coronal 250 Slightly carmine— 2 038 1904 Injected (Berlin blue) 406 E., 16.0 Ch., 40x40x40 Good Formalin P Sagittal 20 (Stain - Haematoxylin Eosin) 3 0.7 1907 Operative. Most—advanced third 423 E., 15.2 Good Formol—Zenker P Transverse 50 Carmine 3 0.85 1904 424 E., 172 Good Formalin P Transverse 50 Carmine 3 10 1904 Double infection. Advanced 492 E, 16.8 Ch, 40 x 40 Exc. Zenker P Coronal 40 Al. coch. v 3 0.7 1911 Injected (India ink) 511 E., 160* Ch., 3?* 32x32 Good Ale. P Sagittal 40 Al. coch. 3 1.1 1911 Head injured. Most advanced in group 670 E, 12.5 Poor Ale. P Sagittal 50 (Stain - Haematoxylin Eosin) 3 10 1913 Tubal Advanced 719 E, 15.0 Ch, 50x50x50 Good Formalin P Trans 40 Al. coch. 2 0.6 1913 Median in group 733 E., Ch., 4Sx40x2S ISO Poor Formalin P Sagittal 50 Al. coch. 2 0.6 1913 Median in group 841 E. 15.0 Ch., 18 x 16x9 Good Formalin P Coronal & Trans, 20 10 (Stain - Haematoxylin Eosin), carmine 2 0.32 1914 Operative. Head cut separately 899 E, 160* Ch. 50 x 18 x IS Good Bouin P Sagittal S0 Al. coch. 3 0,65 1914 Tubal Head injured 991 E. l?.0 Good Formalin P Sag so R, V, Gieson 3 0.9 1914 Advanced 1909 E., 14.6 Good Formalin P Coronal 20 Al. coch,or. G. 1 0.3 1917 Less advanced 2673 E.,15.5 Good Formalin P Transverse 40 Al. coch. 2 0.52 1919 Median in group 4430 E., 14.0 Ch, 51 x40x21 Exc. Corros. acetic P Transverse 15 Al. coch,or. G. 3 0.9 1923 Most—advanced third 5542B E., 16.0 Ch, 37x32x25 Good Formalin P Transverse 40 Al. coch. 2 0.7 1927 Other twin abnormal 5747 E, 15.2 Ch, 32x27x25 Poor Alc.—formol P Sagittal 25 Al. coch. 2 0.25 1928 Least—advanced or middle third 5935A E, 13.5 Ch, 40x30x30 Good Formalin P Coronal 40 Al. coch. 1 0.38 1929 Other twin stunted 6522 E, 13.2* Good Corros. acetic C—P Coronal 10 Al. coch. 3 0.8 7 Middle or most—advanced third 6524 E, 11.7* Exc. Corros. acetic C—P Transverse 10 Al. coch. 1 0.4 ? Least—advanced third 6525 E, 13.8* Exc. Corros. acetic C—P Sagittal 8 Al. coch. 2 0.42 ? Weak staining 6527 E, 14.4* Exc. Corros. acetic C—P Transverse 15 Al. coch. 2 0.67 ? Mechanical damage 6528 E, 13.4* Exc. Corros. acetic C—P Coronal 8 Al. coch. 1 0.33 ? Least—advanced third 6529 E, 15.6* Good Corros. acetic C—P Coronal 10 Al. coch. 2 0.4 .5 Middle third 6533 E, 12.5* Good Corros. acetic C—P Sagittal 6, 8, 10 Al. coch. 2 0.45 ? Middle third 6551 E, 18.0 Poor Formalin p Coronal 40 (Stain - Haematoxylin Eosin) 3 0.8 1932 Tubal 7707 E, 14.5 Ch ,37x32 Exc. Bouin C—P Transverse 10 (Stain - Haematoxylin Eosin), phlox. 2 0.54 1939 Operative. Middle third 8097 E, 15.5 Ch, 37x25x21 Good Formalin C—P Transverse 10 (Stain - Haematoxylin Eosin) 1 0.19 1942 Least advanced in group 8172 E, 16.5 Exc. Bouin C—P Transverse 20 (Stain - Haematoxylin Eosin) 3 0.58 1943 Operative. Very advanced 8235 E, 14.0* Good Bouin C—P Sagittal 10 (Stain - Haematoxylin Eosin) Mallory 2 0.25 1944 Tubal 8355 E, 15.0 Ch, 23 Exc. Formalin C—P Coronal 10 Azan 1946 Tubal. Duplicated spinal cord caudally 8812 E, 12_9 Exc Formalin C—P Transverse 10 (Stain - Haematoxylin Eosin) 1950 Rubella. Medical abortion. Midbrain punctured 8945 E, 13.9 Good Zenker p Transverse 8 Borax, carm. 1952 Univ. Chicago No. H 1254 9107 E, 17.0 Ch, 38x28x22 Good Bouin p Transverse 15 Borax, carm. 1918 Univ. Chicago No. H 516 9247 E, 15.0 Exc. Bouin C—P Sagittal 8 Azan 1954 Tubal Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined. Carnegie Collection - Stage 19  Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Score Sex Year Notes 17 E, 18 Ch, 40x30x20 Poor Alc. P 50, 100 Al. carm. 16.5 Male 1894 43 E, 16 Good Alc. P 50 Al. coch. 10 Male 1894 293 E, 19 Poor Ale. P Sagittal 50 Coch. 16.5 S 1905 390 E, 19 Good Formol? P Sagittal 20, (Stain - Haematoxylin Eosin) 11.5 Male 1906 Tubal Injected 50 409 E.18 Ch, 50x40x40 Good Formalin P Transverse 20 Copper, iron H. & erythrosin 14.5 Male 1907 432 E..18.5 Ch , 45x35x20 Good Formalin P Sagittal 20 H. & Congo red 13.5 Male 1910 Tubal 576 E. 17 Ch, 60x40 Good Formalin P Sagittal 15, 20 (Stain - Haematoxylin Eosin) 14.5 d 1912 Tubal 626 E., 21.5 Ch., 40x30x21 Good Formalin P Transverse 100 Al. coch. 14_5 6 1913 6??8 E, 20 Ch, ca. 30 Poor Formalin P Sagittal 50 Al. coch. 12 9 1913 Head damaged 709 E, 19 Ch. 40x35x25 Poor Alc. P Coronal 40 Al. coch, Lyons blue 15 49 1913 837 E. 21 Ch. 65x45x Good Formalin P Sagittal 40 Al. coch. 14.5 P 1914 1324 E., 18 50x30x18 Good Formalin C Coronal 40 (Stain - Haematoxylin Eosin), aur, or. G 125 79 1915 1332 E., 19 Ch., 40x43x22 Poor Formalin C Coronal 40 (Stain - Haematoxylin Eosin) aur, or. G. 15 Male 1915 1390 E., 18 Ch, 40x38x15 Good Formalin P Sagittal 20 Al. coch. 10_5 Male 1915 Tubal 1534 E., 13 Ch.,35x31x25 Poor Formalin P 53% 50 Al. coch. 13.5 F 1916 Protruding midbrain 2114 E., 19.3 Ch., 49x42x33 Good Formol P Transverse 40 A1. coch. 12 M 1918 4405 E., 15.5 Good Formalin P Transverse 10 Coch, Mallory 13.5 <3 1923 Midbrain injured 4501 E, 18 Exc. Bouin P Transverse 15 Coch, or. G. 14.6 1924 Cystic left kidney 5609 E., 18 Exc. Formalin P Coronal 25 A1. coch. 13.5 Male 6150 E., 17 Ch., 40x39x30 Good Bouin C-P Transverse 15 (Stain - Haematoxylin Eosin) 16.5 Male 1930 Tubal 6824 E., 18.5 Ch., 45x40x25 Good Formalin C-P Sagittal 12 (Stain - Haematoxylin Eosin) 14.5 Female 1933 7900 E., 16.5 Good Bouin C-P Sagittal 20 (Stain - Haematoxylin Eosin), phlox. 11.5 . . 1941 Tubal 8092 E., 16.3 Ch., 52 x 47 Exc. Bouin C-P Transverse 20 (Stain - Haematoxylin Eosin), phlox. 13 Male 1942 8913 E.,? Ch, 34 Poor Formalin p Transverse 10 Alan . 7 1951 rubella. Medical abortion. Isolated head damaged 8965 E, 19.1 Ch, 42x32x19 Good Formol—Zenker C-P Transverse 10 Borax, carm, or. G. 1952 Univ. Chicago No. H 173 9097 E, 21 Exc. Formol—glucose C-P Coronal 10 Azan ? . ? 1930 Univ. Chicago No H 1380 9113 E, 185 Ch, 24 Exc. Formalin C-P Transverse 10 Alan > 6 1953 Rubella. Medical abortion 9325 E, 17.0 Ch, 32x28x20 Good Formalin —acetic p Transverse 15& 8-10 Azan & Ag ? - 1955 Tubal Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined. Carnegie Collection - Stage 20  Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Point Score Sex Year Notes 240 E, 20 Ch, 50x40x30 Poor Formalin P Coronal 20 Iron H. 27 d 19?? 256 E, 21 poor Alc p Sagittal 25 Coch. 23 M 1904 Tubal. Partial anencephaly 353 E, 20 Poor Alc P Sagittal 20 H. & Congo red 22,5 M 1906 431 E, 19 Ch, 30x25x25 Good Formalin P Sagittal 20 H. & Congo red 25,5 M 1908 Tubal 437 E, 23 Ch., 80x60x50 Poor Formalin P Sagittal 50 Coch 24 M 193? 453 E, 23 Ch, 60x40x30 Poor Formalin P Sagittal 20 H. & Congo red 23-5 ? 1910 Injected 460 E, 21 Exc. Bichlor. acetic P Transverse 40 (Stain - Haematoxylin Eosin), coch, 24.5 M 1910 injected 462 E, 20 Ch, 50x40x30 Exc. Formalin P Transverse 40 Al, coch 23.5 F 1910 635B E, 22 Poor Alc P Transverse 50 Al, coch 26.5 M 1913 657 E, 25 Ch, 35x20x15 Poor Formalin C Sagittal 40 Al, coch 26.5 M 1913 Tubal 966 E, 23 Ch, 51x38x13 Exc Bichlor. acetic P Coronal 40 (Stain - Haematoxylin Eosin), aur, or. G 25 M 1911 Tubal 1134B E, 23 Poor Formalin p Sagittal 100 Al. coch., 22 - 1915 1266 E, 23.1 Poor Formalin C-P Sagittal 25 Al. coch., (Stain - Haematoxylin Eosin) aur, or G 20.5 F 191? 2393 Ch, 61.5x50x35 Poor 3527 E., 22 Ch. 32x30x10 Good Formalin P Sagittal 25 Al. coch., 28 ? 1921 4059 E, 21.6 Good Formalin P Coronal 15 Al. coch., Mallory 29.5 $ 1922 4148 E, 21 Ch. 45x34x30 Good Formalin p Coronal 15 A1. coch., Mallory 19 ? 1922 4361 E, 22 Ch., 52x42x23 poor Formalin 9 Transverse 20 Coch. 24 8 1923 6202 E, 21 Ch., 35x35x22 Exc Bouin P Sagittal 20 (Stain - Haematoxylin Eosin) 20.5 8 1930 Tubal 6426 E 21.5 Good Formalin C—P Transverse 20 (Stain - Haematoxylin Eosin) 21 3 1931 7274 E, 18.5 Ch., 48x44x35 Exc Bouin C—P Transverse 20 (Stain - Haematoxylin Eosin), phlox. 20 M 1936 7906 E19.5 Exc Bouin C—P Coronal 20 (Stain - Haematoxylin Eosin) 22 8 1941 Left renal agenesis 8517 E., 20.8 Exc. Bouin C—P Coronal 20 (Stain - Haematoxylin Eosin) 24 8 1943 8226 E, 18.0 Exc. Bouin C—P Sagittal 10 Alan ? 3 1944 Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined. Carnegie Collection - Stage 21  Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Point Score Sex Year Notes 22 E, 20 Ch, 35x30x30 Good Alc. P Transverse 50 Al. coch. 34.5 Female 1895 57 E, 23 Ch., ca. 30 Poor Alc. P Sagittal 50 Al. coch. 36 Male 1896 128 E, 20 Ch., 50x43 Good Formalin P Coronal 50 Al. coch. 33 Female 1898 229 E, 19 Poor Alc. P Sagittal 50 Al. coch. 33 Female 1903 349 E, 24 Good Zenker C Coronal 250 Unstained 36 ? 1905 Double vascular injection 455 E, 24 Ch., 42x34x20 Good Alc. P Transverse 30 (Stain - Haematoxylin Eosin) 36.5 Male 1910 632 E, 24 Ch., 60x50x30 Good Bichlor. acetic P Sagittal 40, 100, 250 Al. coch. 33 Female 1913 Injected 903C E, 23.5 Good Formalin P Transverse 40 Al. coch. 38.5 Female 1914 1008 E, 26,4 Good Formalin P Sagittal 40 Al. coch. 39 ?? 1914 1358F E, 23 Good Formalin P Sagittal 40 Al. coch. 37.5 Female 1916 2937 E,, 24.2 Good Bouin P Transverse 50 (Stain - Haematoxylin Eosin) aur., or. G. 39 Female 1920 3167 E., 24.5 Ch., 60x50x40 Poor Bichlor, acetic, formol P Transverse 20 Al. coch. 32 Male 1920 4090 E, 22.2 Ch.. 66x46x30 Good Formalin P Transverse 40 Al. coch. 30 Female 1922 4160 E,25 Poor Formalin P Sagittal 25 (Stain - Haematoxylin Eosin) 39 Male 1923 Tubal 4960 E.22 Ch,, 47x42x28 Good Formalin P Transverse 15 Al. coch., Mallory 31.5 Female 1925 5??6 E. 215 Good Formalin P Sagittal 20 (Stain - Haematoxylin Eosin) 34 Female 1927 6531 E,22 Poor Glacial acetic, C-P Transverse 10 (Stain - Haematoxylin Eosin) 31.5 Female 1931 Leitz Collection 7254 E,225 Exc Bouin C-P Transverse 20 (Stain - Haematoxylin Eosin) 33.5 Male 1936 7592 E,22-> Exc. Bouin C-P Transverse 20 (Stain - Haematoxylin Eosin) 36 Female 1937 7864 E., 24 Exc, Formalin C-P Frontal 20 (Stain - Haematoxylin Eosin) 32.5 Male 1941 8553 E., 22 Exc Bouin C-P Transverse 12 (Stain - Haematoxylin Eosin) 38 Female 1947 9614 E,,22 5 Exc Bouin P Coronal 10 &15 Azan ? ? 1958 Rubella. Hysterectomy Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined. Carnegie Collection - Stage 22  Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Point Score Sex Year Notes 392 E., 23 Ch., 45x45x25 Poor ... P Sagittal 50 Al. coch. 42 Female 1907 Brödel Collection. Injected 405 E., 26 Good Formalin C Sagittal 40 Carmine 42.5 Male 1907 464 E.,26 Ch., 45x40x30 Good Formol? alc? P Sag. 100 Al. coch. 44.5 Male 1910 584A E.,25 Ch., 50x42x40 Poor Formalin P Sagittal 50 Al. coch. 41 ? 1913 630 E., 25 Poor Formalin P Transverse 100 Al. coch. 46 Male 1913 840 E, 24.8 Good Formalin P Transverse 50 Al. coch. 44.5 Female 1914 875 E, 27 Ch., 40x28x22 Good Formalin P Sagittal 40 Al. coch. 45 Male 1914 895 E., 26 Ch., 67x62x54 Good Formalin P Transverse 25 Al. coch. 46.5 Female 1914 1315 E.,25 Good Formalin P Sagittal 50 Al. coch. 40.5 Female 1915 Spina bifida and anencephaly 1458 E., 27.5 Ch, 45x45x30 Exc. Formalin C Sagittal 50 (Stain - Haematoxylin Eosin) aur, or. G. 45.5 Male 1916 1894 E, 24.6 Good Formalin c Sagittal 40,80 (Stain - Haematoxylin Eosin) aur, or. G. 41 Female 1917 2206 E, 27 Ch, 50x30x18 Poor Formalin p Transverse 40 (Stain - Haematoxylin Eosin) 44.5 Male 1918 3681 E, 26.3 Ch, 36x36x34 Good Formalin p Transverse 25 Al. coch. 44.5 Male 1921 4304 E,25 Ch, 66x45x45 Good Bouin p Transverse 20 (Stain - Haematoxylin Eosin) 44.5 Female 1923 Injected 4339 E, 24.5 Good Formalin p Transverse 15 Al. coch, Mallory 46,5 Female 1923 4476 E., 26.2 Good Bouin p Transverse 40 (Stain - Haematoxylin Eosin) 46 Female 1923 Tubal 4638 E, 23.4 Exc. Bouin p Transverse 15,20 Al. coch, or. G. 41.5 Male 1924 6701 E, 24 Poor Formalin p Coronal 20 (Stain - Haematoxylin Eosin) 41 Female 1933 6832 E, 25.8 Exc. Bouin C-P Coronal 20 (Stain - Haematoxylin Eosin) 42 Female 1934 8394 E, 25.3 Ch, 48x50x34 Exc. Bouin C-P Transverse 20 (Stain - Haematoxylin Eosin), Masson 44.5 Female 1946 8948 E, 26.7 Ch, 61x51x50 Poor Formol-Zenker p Transverse 15 Ag ? ? 1952 Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined. Carnegie Collection - Stage 23  Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Point Score Sex Year Notes 45 E,28 Ch, 40x35x20 Poor ? P Coronal/Transverse 50 Al. coch. 51.5 Female 1895 75 E,30 Good Alc. P Sagittal 50 Coch. 57 Male 1897 86 E,30 Good ? ? Coronal 50 Coch. Male 1897 May be an early fetus 100 E,27 Poor ? P Sagittal 50 Al. coch. 57.5 ? 1897 108 E, 28 (est.) Poor Piurosulph. acid P Sagittal 45 Borax carm. 52.5 Male 1897 227 E30 Ch, 60x40x20 Poor Formalin P Sagittal 50, 100 Al. coch. 54 Female 1903 417 E,32 Ch., 0x60x40 Good Formalin P Transverse 100 Al. coch. 58.5 Female 1907 756A E, 27 Ch. 60x45x35 Good Formalin P Coronal 50 Al. coch. 56 Male 1913 882 E , 28 Ch, 80x80x40 Good Formalin P Transverse 40 Multiple 53 8 1913 950 E, 29 Good Formol P Transverse 50 Al. coch. 54 Male 1914 1199 E.,26 Ch,, 60x40x30 Good Formalin C Coronal 40 (Stain - Haematoxylin Eosin) aur , or. G. 54.5 Male 1915 1535 E , 28 Ch. 50x45 x15 Poor Formalin P Transverse 40 (Stain - Haematoxylin Eosin) 495 Female 1916 1945 E., 27.3 Ch., 83x53x22.5 Good Formalin C-P Transverse 50 (Stain - Haematoxylin Eosin) aur., or. G. 48 Male 1917 2561 E., 27 .5 Good Formalin C-P Transverse 25 (Stain - Haematoxylin Eosin) aur., or. G. 48 .5 Male 1919 4205 E., 29.5 Good Bouin P Transverse 50 A1. coch. 55.5 Female 1923 4289 E., 32.2 Ch., 52x35x25 Good Formalin P Transverse 15, 20 A1. coch., Mallory 59 Female 1923 4525 E., 30 Good Formalin P Sagittal 20 (Stain - Haematoxylin Eosin) 57 Male 1924 4570 E, 30.7 Ch., 52X50X28 Exc. Bouin P Transverse. 15 (Stain - Haematoxylin Eosin) , phlox. 55 Male 1924 5154 E.,32 Good Bouin P Transverse 20 (Stain - Haematoxylin Eosin) 59.5 Male 1926 5422 E., 27 Good Formalin P Sagittal 40 (Stain - Haematoxylin Eosin) 52.5 Female 1927 5621A E., 27.5 Good Formalin P Transverse 20 (Stain - Haematoxylin Eosin) 52.5 Male 1927 Other twin has spina bifida and fused kidneys 5725 E, 23 Good Formalin P Coronal 25 (Stain - Haematoxylin Eosin) aur., or. G. 50.5 Female 1928 6573 E,31.5 Good Bouin C Transverse 20 (Stain - Haematoxylin Eosin) 58.5 Female 1932 7425 E, 27 Exc. Bouin C-P Coronal 20 (Stain - Haematoxylin Eosin) 47 Female 1937 Ag added 9226 E, 31 Exc. Formalin C—P Transverse 12 Azan ? Female 1954 D.122 E, 27 Exc. ? ? Transverse 19 Ag ? ? 1976 Yntema and Truex Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined. Carnegie Collection - Fetal   Serial No. Size CRL (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Point Score Sex Year Notes 95 40 catalogued as CRL 40 but development suggests 50 stage. Spinal cord - Kunitomo (1920) Colon - Lineback (1920) 96 50 Brain venous sinuses - Streeter (1915) Spinal cord - Kunitomo (1920) Brain vascular - Streeter (1921) Brain weight - Jenkins (1921) 142 125 Spinal cord - Kunitomo (1920) 145 33 Spinal cord - Kunitomo (1920) 184 50 34 vertebrae, 31 spinal ganglia, Spinal cord - Kunitomo (1920) 211 33 34 vertebra, 31 spinal ganglia, Spinal cord - Kunitomo (1920) 217 45 Male Genital - Spaulding (1921) 300 73 85 days, Bone ossification - Mall (1906) 362 30 Spinal cord - Kunitomo (1920) 448 52 Colon - Lineback (1920) 449 36 Spinal cord - Kunitomo (1920) 538 590 21 to 23 Male Genital - Spaulding (1921) 607 37 Male Genital - Spaulding (1921) 625 220 Temporomandibular joint - Moffatt (1957) 662 80 Spinal cord - Kunitomo (1920) 693 45 Male Genital - Spaulding (1921) 847 58.8 Male Genital - Spaulding (1921) 858 57.25 Temporomandibular joint - Moffatt (1957) 922 37 928 120 Spinal cord - Kunitomo (1920) 948 45 Male Genital - Spaulding (1921) 972 37 34 vertebrae, 30 spinal ganglia, Spinal cord - Kunitomo (1920) 1318 37 Temporomandibular joint - Moffatt (1957) 1388 51 Female Genital - Spaulding (1921) 1455 78.5 Temporomandibular joint - Moffatt (1957) 1591 36 subcutaneous vascular plexus - Finley (1923) 1656 67 34 vertebrae, Spinal cord - Kunitomo (1920) 1686 40 Male Genital - Spaulding (1921) 3990 49 Temporomandibular joint - Moffatt (1957) 4473 43 20 Spinal cord meninges - Sensenig (1951) 4475 48 20 Spinal cord meninges - Sensenig (1951) 5652 49 Temporomandibular joint - Moffatt (1957) 6581 75 Temporomandibular joint - Moffatt (1957) 7218 80 20 um Spinal cord meninges - Sensenig (1951) 1597b 47 Female Genital - Spaulding (1921) 2250a 40 Female Genital - Spaulding (1921) 2250b 36 Female Genital - Spaulding (1921) This table currently contains only has embryo number information. Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined. References Fertilization and Gestational Age - Crown-Rump Length (ultrasound)  Fertilization Age (days) Gestational Age GA (week.day) Crown-Rump Length (mm) 37 5.2 1 38 5.3 2 39 5.4 3 40 55 3 41 5.6 4 42    Week 4 6 4 43 6.1 5 44 6.2 6 45 6.3 7 46 6.4 8 47 6.5 9 48 6.6 10 49    Week 5 7 11 50 7.1 11 51 7.2 12 52 7.3 12 53 7.4 13 54 7.5 14 55 7.6 15 56    Week 6 8 17 57 8.1 18 58 8.2 19 59 8.3 20 60 8.4 21 61 8.5 22 62 8.6 22 63    Week 7 9 23 64 9.1 24 65 9.2 26 66 9.3 27 67 9.4 28 68 9.5 29 69 9.6 31 70    Week 8 10 34 71 10.1 36 72 10.2 37 73 10.3 38 74 10.4 39 75 10.5 39 76 10.6 40 77    Week 9 11 44 78 11.1 45 79 11.2 47 80 11.3 48 81 11.4 52 82 11.5 55 83 11.6 56 84    Week 10 12 57 85 12.1 58 86 12.2 60 87 12.3 61 88 12.4 63 89 12.5 64 90 12.6 65 91    Week 11 13 68 92 13.1 70 93 13.2 72 94 13.3 74 95 113.4 76 96 135 77 97 13.6 80 98    Week 12 14 81 99 14.1 84 100 14.2 85 101 14.3 86 102 14.4 87 Reference: Table data measured by ultrasound, adapted from Westerway (2015) PDF and Links: ultrasound | Fetal Development

Week:	1	2	3	4	5	6	7	8
Carnegie stage:	1 2 3 4	5 6	7 8 9	10 11 12 13	14 15	16 17	18 19	20 21 22 23

NMHM Seminar 2015

Links: First slide | Seminar Slides | Digital Embryology Consortium - Information | Digital Embryology Consortium

Origin of the Collection

The following text is an excerpt from: Mall FP. and Meyer AW. Studies on abortuses: a survey of pathologic ova in the Carnegie Embryological Collection. (1921) Contrib. Embryol., Carnegie Inst. Wash. Publ. 275, 12: 1-364. Care and Utilization of the Collection

The collection of human embryos belonging to the Carnegie Institution of Washington owes its origin to thirty years of untiring effort on the part of one of the authors (Mall). The first specimen was obtained while he was a student under Professor Welch in the Pathological Department of the Johns Hopkins University; very soon another, in excellent state of preservation, was added. After his (Mall's) transfer to Clark University in 1899, embryo No. 2 was studied and modeled in wax. This was the first reconstruction of a human embryo ever made in America and at that time the most elaborate one in existence. In 1890 this specimen was offered to Professor His, who refused to accept the gift, and returned it, together with several from his own collection, expressing the hope that this small number of specimens might serve as a nucleus for a much larger collection. With the subsequent foundation of the University of Chicago, the collection was transferred there, and during the following year a few additions were made. Now somewhat augmented, it was returned to Baltimore in 1893, at the opening of the Johns Hopkins Medical School, and here it grew for a number of years, at first slowly, then more rapidly, until it was finally taken over by the Carnegie Institution of Washington in 1915.

In the beginning each specimen was labeled with the name of the physician who donated it, but it was soon found that this method was not accurate. Bottles were easily misplaced and notes lost from the files were not missed unless marked with consecutive numbers. Therefore, after the collection had grown to about 100 specimens, a system of numbering, somewhat in the order of accession, was adopted. However, a review of the catalogue later disclosed the fact that some of the specimens collected at Worcester followed in numerical order those collected at Chicago, so that for the first 100 specimens the sequence of accession can not be viewed as chronologically reliable.

From an examination of table 1, in which the specimens are arranged in hundreds (or centuries), it will be observed that the first century includes catalogue numbers 1 to 98, the second century, 99 to 205, etc. These numerical discrepancies are due to the fact that quite frequently the same number is given to two or more specimens, as illustrated in the first century; or, as illustrated in the second century, a number once used may be discarded subsequently because the specimen is found to contain no remnants of an ovum. The latter specimens are finally marked on the catalogue card "No pregnancy." In this way we have been able to retain in the catalogues of the collection ovaries and uteri from non-pregnant women.

The second column of the table shows the time required to collect each 100 specimens. It took 10 years for the first, 4 for the second, and 2 for the third 100; but after the collection had been transferred to the Carnegie Institution, about 400 specimens were collected in one year. It will be observed also that approximately 60 physicians contributed to the collection of each 100 specimens, of which number 8 (or about 13 per cent) resided in hospitals. The last column of the table shows the territorial source of each century, representing an average of 18 States from which material has been drawn. Upon comparing columns 2 and 3, it becomes apparent that by far the largest portion of the collection has come from physicians in private practice. Yet nearly all of our perfect specimens were obtained from hospitals. This is easily understood when one considers that only when the operator is near can perfectly fresh embryos be secured. In several instances these have been brought to the laboratory still living.

The Carnegie Institution of Washington 1915

Carnegie Institution of Washington

Founded by Andrew Carnegie

"To encourage in the broadest and most liberal manner investigation, research, and discovery, and the application of knowledge to the improvement of mankind."

Plan and Scope of the Institution

The Carnegie Institution of Washington was founded by Andrew Carnegie on January 28, 1902, when he gave to a board of trustees an endowment of registered bonds of the par vahie of ten million dollars; to this fund he added two million dollars on December 10, 1907, and ten million dollars on January 19, 1911; so that the present endowment of the Institution has a par value of twenty-two million dollars, yielding an annual interest of five percent on this amount. The Institution was originally organized under the laws of the District of Columbia and incorporated as the Carnegie Institution, but was reincorporated by an act ot the Congress of the United States, approved April 28, 1904, under the title of The Carnegie Institution o! Washington.

Organization under the new Articles of Incorporation was effected Mav 18, 1904, and the Institution was placed under the control of a board of twenty-four trustees, all of whom had been members of the original corporation. The Trustees meet annually in December to consider the affairs of the Institution in general, the progress of work already undertaken, the initiation of new projects, and to make the necessary appropriations for the ensuing year. During the intervals between the meetings of the Trustees the affairs of the Institution are conducted by an Executive Committee chosen by and from the Board of Trustees and acting through the President of the Institution as chief executive officer. The Articles of Incorporation of the Institution declare in general "that the objects of the corporation shall be to encourage in the broadest and most liberal manner investigation, research, and discovery, and the application of knowledge to the improvement of mankind." Three principal agencies to forward these objects have been developed. The first of these involves the formation of departments of research within the Institution itself, to attack larger problems requiring the collaboration of several investigators, special equipment, and contributoin ctlort. 1 he second provides means whereby individuals may imdcrrakc and carry to completion investigations not less important but requiring less collaboration and less s|)cci.il rcpiiiMm-iii . The third agency, namely, a division dcxotcd to editing .nul printing books, aims to provide adequate publication of the results oi research coming from the first two agencies and to a limited extent also for worthy works not likely to be published under other auspices.

Summarily, the work of the Institution may be classified under the heads of a Division of Administration, a Division of Publications, Departments of Research, and a Division of Research Associates. The Division of Administration consists of nine persons and is charged with the executive, financial, and correspondence duties of the Institution. The Division of Publications consists of three members permanently employed, and has charge of the work of editing and printing books. Temporary assistance in connection with illustrations, proof-reading, etc., is also employed by this division as needed. The staffs of these two divisions are given on page 9. The Institution has thus far established eleven of the larger departments of research; their designations, the names and addresses of their Directors, the investigatory staffs, and brief indications of their origin, development, and present status are given in the following pages. Many grants have been made in aid of minor projects for investigation, and many Research Associates and collaborators, connected mostly with colleges and universities, have been and are carrying on work also under the auspices of the Institution.

A condensed history of the origin, development, and growth of the Institution will be found in the President's Report contained in the Year Book for 191 1, which also gives lists of all persons who had been engaged up to that time in the work of the Institution from the time of its organization. A more comprehensive view of this history may be gained from the contents of the Year Books and from the other more formal publications issued by the Institution. General and classified lists of these publications may be had on application, and the publications themselves may be found in nearly all of the greater libraries of the world. About three hundred volumes have been issued up to date.

The executive offices of the Institution are in its Administration Building, on the southeast corner of Sixteenth and P streets, northwest, Washington, D. C. This building is constructed of Bedford limestone, is three stories in height, and has an available floor space of about 21,000 square feet. The basement is devoted chiefly to file rooins and to rooms for the receipt, shipment, and storage of the pubhcations of the Institution, with fireproof vaults and shelving provided for such storage. The second floor is supplied with board and committee rooms for the use of the Trustees and the Executive Committee, and with an Assembly Room having a seating capacity for about two hundred persons. The third floor has twelve rooms, furnishing adequate quarters for the Division of Administration and the Division ot Publications.

Department of Embryology (1915)

Director, Franklin P. Mall.

Address, Johns Hopkins Medical School, Baltimore, Maryland.

1915 Investigatory Staff.

Franz Keibel. G. L. Streeter.

H. M. Evans. M. Reicher.

A grant in aid of embryological research was made in the spring of 1913 to Professor Franklin P. Mall, who took immediate steps to organize a laboratory of sufficient magnitude to carry on selected problems of broad scope, particularly such as are beyond the reach of a single individual. During 1913 and 1914 he associated with him for this work the staff named above.

On December 11, 1914, the estabhshment of a Department of Embryology was authorized by the Board of Trustees and Professor Mall was appointed Director. Researches at present are being carried on in a suite of rooms in the Anatomical Laboratory of the Johns Hopkins University and its facilities thus available are unusually advantageous tor embryological investigation. Competent support has been secured from scientific assistants, sjieciallv trained technicians, artists, and modelers.

During the past year Dr. Mall has transferred to the Institution his embryological collection, the result of his unceasing efforts during the past tw enty-seven years. It consists of over 1,000 human specimens, many of which have been prepared in permanent serial sections. fhe collection is already unicjue in both magnitude and imj)ortance, but a vigorous effort is being made to still further increase it. It is now safely housed in fire-proof rooms, together with the original data, drawings, photographs, and clinical records, which are second in importance only to the specimens themselves. Convenient classified lists and a card catalogue have been prepared to render all of the material easily axailable.

Under general embryology, work is progressing to establish a norm tor the external form of embrxos under 25 millimeters long, together with a dixisioii ol this period into stages, ami phvsicoanthropologic measuremeius arc being ni.ule to extend this work to older fetuses. This will add greater precision to the determination of the age of embryos and will help explain anatomical wiriations and tlie characteristics in racial anatomy. Considerable attention is being directed to the pathological asj)ects of embryology and their bearing on fertilization, duration of pregnancy, and the causes of abortion and sterility. A study of the fate of the ovum in tubal pregnancy is now in press.

The structural anatomy of the embryo at different stages is being studied. Models and drawings have been finished of embryos between 2 and 4 millimeters. A study of the development of the muscles of the head region is nearly completed. A monograph on the structure of the medulla oblongata has been published. A study of the development of the abdominal veins is practically ready for publication, making an important link in the series of investigations on the formation of the vascular systems which have been under way for the past three years. A volumetric study of the embryo brain is nearing completion and also a study concerning the drainage of the developing membranous labyrinth. Among the cytological studies mention should be made of the papers recently published regarding the behavior of somatic cells toward vital stains.

Department of Embryology (1929)

From left to right: Warren H Lewis, George L Streeter, Martin Nordmann, Emanuel Fauré-Fremiet, Zygmunt Grodzinski, RH Hunter, Chester H Heuser, Carl G Hartman and Otto Leif Tinklepaugh.

Carnegie Collection Embryos

iBook - Carnegie Embryos

iTunes link | iBook Store Description - Imagine the excitement of seeing this incredible early period of human development for the first time. Now consider that much of our initial understanding of human development is based upon study of historic embryo collections. You can now look at these historic images of the first 8 weeks after fertilisation and explore for yourself the changes that occur in human development during this key period. This current book is designed as an atlas of the Carnegie embryo stages with some brief notes and additional information covering the first 8 weeks of development. These images are from from the beginning of last century and are one of the earliest documented series of human embryos collected for basic research and medical education on development. I hope you enjoy learning about the amazing early events that begin to make and shape us. This is the second book in a series of educational releases from UNSW Embryology. Release: First Edition - Mar 12, 2012 ISBN 978-0-7334-3148-7 Print Length 82 Pages, 25.8 MB Language English. PDF Preview version 3.87 MB (Read the associated information, this is an edited educational preview version with many features not functioning). The current website also includes numerous embryo images from this textbook (see Embryonic Development and Carnegie Embryos).

Online Editor

Each Carnegie embryo was allocated a specific catalogue number within the collection. Within the online site this number links to a "category" with more detailed information about that embryo. This includes the original characterisation, fixation, plane of sectioning, section thickness, staining, whether normal/abnormal/tubal and any additional comments. There is also a list of references that have studied that specific embryo. The category will also show other embryos of that stage as well as stage related links.

Stage 3

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  Embryo No. 8794

• 

  Embryo No. 8663

Stage 5

• 

  Embryo No. 8155

• 

  Embryo No. 8155

• 

  Embryo No. 8155

• 

  Embryo No. 8004

• 

  Embryo No. 8004

• 

  Embryo No. 8004

• 

  Embryo No. 7700

• 

  Embryo No. 7700

• 

  Embryo No. 7700

• 

  Embryo No. 7700

Stage 7

• 

  Embryo 7802

• 

  Embryo 7802

• 

  Embryo 7802

• 

  Embryo 7802

Carnegie Collection - Stage 7
Serial No.	Grade	Fixative	Embedding Medium	Thinness (µm)	Stain	Year	Notes
7802	Exc.	Alc. & Bouin	C-P	6	(Stain - Haematoxylin Eosin)	1940	Heuser et al. (1945)
8206	Good	p	C-P	6	(Stain - Haematoxylin Eosin)	1943
8361	Good	Bouin	C-P	10	p	1946	Abnormal
8602	Exc.	Alc.	C-P	8	(Stain - Haematoxylin Eosin)	1948
8752	Exc.	?	C-P	10	(Stain - Haematoxylin Eosin)	1950
8755	Exc.	Formol	C-P	10	(Stain - Haematoxylin Eosin)	1950
9217	Exc.	p	P	10	(Stain - Haematoxylin Eosin)	1954
Abbreviations Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined.

Stage 8

• 

  Embryo 5960

• 

  Embryo 5960

• 

  primitive pit

• 

  Embryo 7545 primitive groove and primitive streak

• 

  Embryo 7545 notochordal process and notochordal canal

• 

  Embryo 7545 section series

• 

  Embryo 7545 notochordal process and notochordal canal

Embryo 7545: primitive pit | primitive groove and primitive streak | Notochordal process and notochordal canal | section series | Notochordal process and notochordal canal

Carnegie Collection - Stage 8
Serial No.	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Year	Notes
1399	Poor	Formol	P	Transverse	10	(Stain - Haematoxylin Eosin) etc.	1916	"Mateer embryo" described by Streeter (1920a)
3412	Poor	Formol	P	Transverse	5-15	Al. coch. E. au., or.	1921
5960	Good	Kaiserling	P	Transverse	5	Al. coch. & eosin	1929	Heuser (1932b)
6630	Poor	Formol	P	Oblique	6	(Stain - Haematoxylin Eosin)	1932
6815	Poor	Formol	P	Oblique	10	Al. coch., or. G	1933
7170a and b 7545	Poor	Alc.	C-P	Transverse	6	(Stain - Haematoxylin Eosin)	1935	Twins
7568	Poor	Formoi	C-P	Transverse	6	(Stain - Haematoxylin Eosin)	1938
7640	Good	Formol & Bouin	P	Transverse	10	(Stain - Haematoxylin Eosin)	1939	George (1942)
7666	Exc.	Formol-chrom. subl.	C-P	Transverse	6	(Stain - Haematoxylin Eosin)	1939	"H. 1515"
7701	Exc.	?	C-P	Transverse	8	(Stain - Haematoxylin Eosin)	1939
7822	Good	Formoi	C-P	Transverse	10	(Stain - Haematoxylin Eosin)	1940
7949	Good	Zenker	p	Sagittal	10	(Stain - Haematoxylin Eosin) etc.	1941
7972	Good	Alc. & Bouin	C-P	Sagittal	6	(Stain - Haematoxylin Eosin)	1942
8255	Exc.	Bouin	C-P	Sagittal	8	(Stain - Haematoxylin Eosin), phlox.	1944	Slides showing embryo returned to Dr. Patten in 1962
8320	Good	Formol	C-P	Sagittal	8	(Stain - Haematoxylin Eosin), phlox.	1945
8352	Good	Formol	C-P	Transverse	8	(Stain - Haematoxylin Eosin), phlox.	1946
8371	Poor	Alc. & Bouin	C-P	Sagittal	8	(Stain - Haematoxylin Eosin), phlox.	1946
8671	Exc.	Alc. & Bouin	C-P	Sagittal	6	(Stain - Haematoxylin Eosin), phlox.	1949
8725	Exc.	Alc. & Bouin	C-P	Sagittal	6	(Stain - Haematoxylin Eosin), phlox.	1949	Preparation method described by Heard (1957)
8727	Exc.	Alc. & Bouin	C-P	Transverse	8	(Stain - Haematoxylin Eosin), phlox.	1949	Germ disc folded, possibly double (Hertig, 1968, fig. 180)
8820	Good	Zenker-formol	?	Transverse	10	(Stain - Haematoxylin Eosin)	1951	"Jones-Brewer I" (H. 1459) described by Jones and Brewer (1941)
9009a and b 9123	Good	Formol	C-P	Sagittal	6	(Stain - Haematoxylin Eosin)	1952	Twins described briefly by Heuser (1954)
8371	Good	Formol	C-P	Sagittal	6	(Stain - Haematoxylin Eosin)	1953
9251	Good	?	C-P	Sagittal	10-12	Azan, H. & phlox.	1954
9286	Exc.	Formol	C-P	Transverse	8	Azan	1955
10157	Exc.	Formol	C-P	Transverse	?	Cason	1967
10174	Exc.	Bouin	p	Transverse	8	Cason	1967
Abbreviations Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined.
References

Stage 9

• 

  Embryo 1878

• 

  Embryo 1878

• 

  Embryo 1878

• 

  Embryo 1878

• 

  Embryo 1878

• 

  Embryo 1878

• 

  Embryo 1878

• 

  Embryo 1878

Carnegie Collection - Stage 9
Serial No.	Pairs of somites	Size (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Year	Notes
1878	2-3	Embryo, 1.38 Ch., 12x10.5x7.5	Good	Formalin	P	Coronal	10	(Stain - Haematoxylin Eosin)	1917	Described by Ingalls (1920).
5080	1	Embryo, 1.5 Ch., 14.5	Poor	Formalin	P	Transverse	10	Al. coch.	1926	Studied by Davis (1927).
7650	2-3	Embryo, 2-3	Good	Alc & Bouin	C-P	Transverse	6	(Stain - Haematoxylin Eosin)	1939	Said to be female
Abbreviations Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined.
References

Stage 10

• 

  6330 dorsal

• 

  6330 lateral

• 

  7251 lateral

• 

  3710 lateral

Carnegie Collection - Stage 10
Serial No.	Pairs of somites	Size (mm)	Grade	Fixative	Embedding Medium	Thinness (µm)	Stain	Year	Notes
391	8	E, 2 Ch., 14	Good	Formalin	P	10	Al. coch.	1907	Monograph by Dandy (1910)
1201	7	E,2 Ch.. 144	Good	Formalin	P	8	H. & or. G.	1915	Univ. Chicago No. H 87
2795	4-5	E,2	Poor	Alc.	P	6	Al coch,or.G.	1919
3707	12	E, 1 5	Good	Formalin	P	12.5	I. H.	1921	Univ. Calif. No. H 197
3709	4	E. 1.4 Ch.. 14.8	Poor	Formalin	P	10	Erythrosin	1921	Univ. Chicago No H 279
3710	12	E., 3.6 Ch., 19.0	Good	Formalin	C-P	10	H. & or. G.	1921	Univ. Chicago No. H 392
4216	8	E, 2 Ch, 9.8	Good	Formalin	P	15	?	1923	Monograph by Payne (1925)
5074	10	E., 3.3 Ch., 10.8	Exc.	Bouin	P	10	Al. coch.	1925	Univ. Rochester No. H 10. Monograph by Corner (1929)
6330	7	E, 2.83	Good		P	5	Ehr. H.	1931	Univ. Chicago No. H 1404
6740	12	E., 2.2	Good	p	C-P	8	?	1933	Litzenberg embryo. Studied by Boyden (1940)
7251	8	E., 1.27	Good	Formalin	C-P	10	(Stain - Haematoxylin Eosin)	1941	"Singapore embryo." Univ. Cambridge No. H 98. Studied by Wilson (1914)
8244	6	E., 1.55 Ch, 8,5	Good	Alc.	C-P	8	(Stain - Haematoxylin Eosin) phlox.	1944
9870	12	Ch, ca. 8	Good	Zenker	P	5	Various, chiefly carmine	1952	Univ. Chicago. No. H 637. Dicephaly
Abbreviations Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined. All Carnegie stage 10 embryos are cut in Transverse plane.

Stage 11

• 

  6344 dorsolateral

• 

  6344 dorsal

• 

  6784 ventral

• 

  6050 right

Carnegie Collection - Stage 11
Serial No.	Pairs of somites	Size (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Year	Notes
12	14	E, 2.1 Ch, 13	Poor		P	Transverse	10	Al. carm.	1893
164	18	E, 3.5 Ch, 14	Good	Formalin	P	Transverse	20	Al. carm.	1913
318	13/14	E, 2.5 Ch, 16	Good		P	Transverse	25	Al. carm.	1905
470	17	E, 4.3 Ch, 16	Good	Formalin	P	Transverse	10	Al. carm. .	1910
779	14	E, 2.75	Good		C	Transverse	15	Al. coch.	1913	Dysraphism. Noted by Dekaban (1964)
1182b		E, 3 Ch, 15x12x5	Good	Formalin	?	Transverse	20	Al. carm.	1915
2053	20	E, 3.1 Ch, 12	Exc.	Formalin	P	Transverse	10	Al. coch.	1918	Most advanced in group. Ag added to slide 2 Monographs by Davis (1923) and Congdon (1922)
4315	17	E, 4.7 Ch, 23x10.4X11	Excellent	?	C-P	Transverse	10	I.H. & E.	1923	Univ. Chicago No. 951. Wen (1928)
4529	14	E, 2.4 Ch, 21	Excellent	Formalin	P	Transverse	10	Al. coch, or. G.	1924	Heuser (1930)
4783	13	E, 2.3	Fair	?	?	Transverse	5	I.H.	1924	Wallin (1913)
4877	13	E, 2 Ch, 15	Good	Formalin	P	Transverse	15	Al. coch.	1925
5072	17	E, 3	Good	Formalin	P	Transverse	10	(Stain - Haematoxylin Eosin)	1925	Tubal Type specimen. Atwell (1930)
6050	19/21	E.,3 Ch, 10	Good	Formalin	C-P	Coronal	10	Al. coch.	1930	Advanced
6344	13	E, 2.5 Ch, 17	Excellent	Formalin	C-P	Transverse	6	Al. coch.	1931	Least advanced in group
6784	17	E, 5 Ch, 16	Excellent	Formalin	C-P	Transverse	6	I.H, or. G.	1933
7358	16	E, ? Ch, 15	Poor	Alc, formol	p	Oblique	25	(Stain - Haematoxylin Eosin)	1936
7611	16	E., 2.4 Ch., 12	Excellent	Bouin	C-P	Transverse	8	(Stain - Haematoxylin Eosin)	1938
7665	19	E., 4.36	Excellent	?	C-P	Transverse	6		1939	Univ. Chicago No. H 1516
7702	17	E, 3.7 Ch., 14	Good	Formalin	C-P	Transverse	10	Al. coch.	1940	Returned to B M Patten
7851	13	E., 4.3 Ch, 18	Excellent	Formalin	C-P	Transverse	8	(Stain - Haematoxylin Eosin)	1940	Slightly injured
8005	16/17	E, 3	Excellent	Bouin	C-P	Transverse	8	(Stain - Haematoxylin Eosin)	1942	Tubal
8116	17	E, 14 Ch.. 17	Good	Formalin	p	Sagittal	8	Azan	1953
8962	15	E, 1.55	Good	?	*	Sagittal	?	?	1952	Tubal Univ. Chicago No. H 810
Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined.
References

Stage 12

• 
• 
• 
• 
• 

Carnegie Collection Embryos - Stage 12
Serial No.	Pairs of somites	Size (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Year	Notes
209	ca_24	EH3 Ch.,15	Poor	Alc	P	Coronal	50	Al. coch.	1902
250	19?	E , 2 (11, 10x9x9	Poor	p	?	Sagittal	20	Al. coch.	p
384	P	E 2_5 Ch.,13	Poor	Formalin	P	Transverse	10	H.&E.	1907	Macerated. Narrow yolk stalk
486	21	E.,4 Ch., 22	Good	Corros. acetic	P	Transverse	10	Al. Coch.	1911
1062	29	E.,4.5 Ch., 20	Good	Formalin	P	Transverse	20	Al. coch.	1915	Transitional to next stage
2197	?	E_,5_3 Ch., 19.5	Poor	Formalin	P	Transverse	10	Al. coch, or. G.	1918
4245-7	ca.24	E.,3.5 Ch., 24	Good	Alc, formol	P	Transverse	10	Al. coch.	1923	Caudal neuropore widely open
4479	P	E.,5 .8 Ch , 17	Poor	Formalin	P	Transverse	40	Al. coch.	1923	Macerated. Upper lirnb buds not visible
4736	26	E.,3.0 Ch.,20	Good	Formalin	P	Coronal	10	Al. coch.	1924	No upper limb buds. Caudal neuropore closed
4759	?	E.,4.5 Ch.,15	Good	Formalin	P	Transverse	15	(Stain - Haematoxylin Eosin)	1924	Neural tube folded
4784	23	E_,3	Good	P	P	Transverse	10	p	1924
5035	25-28	E.,3.8 Ch.,18	Good	Formalin	C-P	Transverse	10	Al. coch.	1925
5048	ca_25	E_,3_5	Good	Formalin	C-P	Transverse	10	Al. coch.	1925	Tubal Injured
5056	25	E,,3 Ch.,12	Good	Formalin	P	Transverse	10	Al. coch.	1925
5206	?	E.,4 Ch., 51x31x30	Poor	??	P	Transverse	20	Al. coch.	1926	Tubal
5300	?	E., 4.5 Ch,16.5	Poor	Formalin	P	Transverse	20	Al. coch.	1926	Autopsy. Partly macerated
5923	28	E.,4 Ch.,15	Exc.	Formalin	P	Transverse	10	Al. coch.	1929
6097	25	E,3.4 Ch., 12.5	Exc.	Formalin	C-P	Transverse	10	Al. coch, eosin	1930	Tubal Ag added to slides 1-3
6144	27	E. 3.3 Ch.,11	Good	Lysol—Zenker	C—P	Transverse	10	Al. coch.	1930
6488	28	E, 32 Ch,22	Good	Formalin	C—P	Transverse	10	Al. coch.	1932
6937	26	E.,3 Ch , 12	Poor	Formalin	C—P	Coronal	10	I.H.,or.G.	1934	Tubal Caudal neuropore closed
7724	ca.29	E,3.5 Ch.,18	Good	Formalin	C—P	Sagittal	8	(Stain - Haematoxylin Eosin)	1940	Caudal end broken
7852	25	E , 3.7 Ch,26	Exc.	Formalin	C—P	Transverse	10	(Stain - Haematoxylin Eosin)	1940	Typical for stage 12
7999	ca.28	E,3.2 Ch , 15	Exc.	Bouin	C-P	Transverse	10	(Stain - Haematoxylin Eosin)	1942	Caudal defect
8505a	24	Ch, 23.5	Exc.	Formalin	C-P	Transverse	P	H. Phlox.	1947
8505b	23	Ch,24	Exc.	Formalin	C-P	Sagittal	p	Azan	1947	Twins
8941	28	E,4.9 Ch, 35	Exc.	Zenker	C-P	Transverse	6 I.H.	1927	Univ. Chicago No. H 1261
8942	25	E, 38 Ch, 35	Exc.	Formal-Zenker	C-P	Coronal	5 11-1.	1930	Univ. Chicago No. H 1382
8943	22	E. 3.9 Ch, 20.4	Exc.	Formal-Zenker	C-P	Transverse	8	H.&E.	1934	Univ. Chicago No. H 1481
8944	25	E,4 Ch,,25	Exc.	Formal-Zenker	C-P	Sagittal	8	I.H.	1936	Univ. Chicago No. H 1514
8963	22	E, 3.8 Ch , 14.5	Fair	Formalin	C-P	Transverse	10	I.H.	1928	Univ. Chicago. No. H 1093 Studied by Wen (1928)
8964	23	E,2.8 Ch - 25	Poor	Formalin	p	Transverse	8	I.H.	1928	Univ_ Chicago No. H984 Studied by Wen (1928)
9154	24	E, 5 4	Exc	Formalin	C-P	Transverse		I.H. & phlox.	1953
Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined.
References

Stage 13

• 

  6473 left

• 

  6473 dorsal

• 

  6473 right

• 

  6469 right

• 

  8066 dorsal

• 

  8066 left

• 

  8119 left

• 

  7433 right

• 

  7433 ventral

Carnegie Collection Embryos - Stage 13
Serial No.	Size (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Year	Notes
1	E.,4.5 Ch., 30x30	Poor	Salicylic acid	P	Transverse	10	Hemat.	1887	Obtained by Mall while student
19	E., 5.5 Ch., 18x14	Poor	p	?	Transverse	20	Al. coch.	1895
98	E., 4 Ch., 24x16x9	Poor	p	P	Transverse	20	Al. coch.	1896
76	E., 4.5 Ch., 22x20	Poor	Alc.	P	Transverse	20	Al. coch.	1897
112	E., 4	Poor	p	P	Sagittal	10	Al. coch.	p
116	E., 5	Poor	p	?	Sagittal	10	Al. coch.	1898
148	E.,4.3 Ch., 17x14x10	Poor	Alc.	P	Coronal	10	(Stain - Haematoxylin Eosin)	1899	Abnormal. Nasal discs fused
186	E.,3.5 Ch., 25x20x15	Poor	Alc.	P	Transverse	20	Al. coch.	1901
239	E., 3.0	Poor	Formalin	P	Transverse	10	(Stain - Haematoxylin Eosin)	1903
248	E., 4.5 Ch., 30x23x15	Poor	p	?	Coronal	50	Al. coch.	1904
407	E.,4 Ch., 14x13X7	Poor	Formalin	?	Transverse	40	Al. coch.	1907
463	E., 3.9 Ch., 17x12x7	Good	Formalin	P	Coronal	10	Al. coch.	1910
523	E., 5 Ch., 25x25x15	Fair	Formalin	P	Transverse	p	Al. coch.	1911
588	E., 4.0 Ch., 19x15x8	Good	Corros. acetic	P	Coronal	15	(Stain - Haematoxylin Eosin)	1912	Advanced
786	E., 4.5 Ch., 19x10x10	Poor	Alc.	P	Sagittal	15	Al. coch.	1913
800	E., 6.0	Good	Corros. acetic	P	Transverse	10	H	1913	Curettage. Anencephaly
808	E.,4.0	Poor	Corros. acetic	P	Transverse	15	Al. coch.	1914	Tubal Incomplete
826	E., 5.0 Ch., 13x13x9	Good	Formalin	P	Transverse	20	Al. coch.	1914	Shrunken
836	E.,4.0 Ch., 22x18x11	Exc.	Corros. acetic	P	Transverse	15	Al. coch.	1914	Less advanced
963	E.,4.0 Ch., 23x18x16	Good	Formalin	P	Coronal	20	Al. coch.	1914
1075	E.,6.0 Ch., 46x32x20	Exc.	Corros. acetic	P	Coronal	20	(Stain - Haematoxylin Eosin) or. G.	1915	Most advanced in group
3956	E., 4.0	Poor	Formalin	P	Transverse	20	Al. coch.	1922	Tubal Incomplete
4046	E.,5 Ch., 22x20x20	Poor	Formalin	P	Transverse	50	Al. coch.	1922
5541	E., 6.0 Ch., 35x30x20	Good	Formalin	P	Transverse	10	Al. coch., eosin	1927
5682	E., 5.3 Ch., 29x25x13	Poor	Formalin	P	Coronal	20	Al. coch.	1928
5874	E., 4.8	Exc.	Bouin	P	Transverse	10	(Stain - Haematoxylin Eosin)	1929	Hysterotomy. Bromides only
6032	E., 5.8 Ch., 30x24x13	Poor	Formalin	P	P	?	p	1929	Not good enough to cut
6469	E., 5.0 Ch., 25x18x18	Poor	Formalin	P	P	P	P	1932	Fragmented on cutting. Not saved
6473	E., 5.0 Ch, 30x30x15	Exc.	Formalin	C-P	Coronal	6	Al. coch.	1932	Less advanced. Ag added
7433	E., 5.2 Ch., 15x13x13	Exc.	Formalin	C-P	Coronal	8	(Stain - Haematoxylin Eosin)	1937	Tubal
7618	E, 48 Ch, 18x15x15	Exc.	Bouin	C-P	Coronal	10	(Stain - Haematoxylin Eosin)	1939	Hystereaomy. Advanced. Ag added
7669	E, 5.0 Ch., 23x16x14	Good	Formalin	C-P	Coronal	6	(Stain - Haematoxylin Eosin)	1939	Hysterectomy. Least advanced in group, Ag added
7889	E, 4.2	Exc.	Bouin	C-P	Coronal	6	(Stain - Haematoxylin Eosin)	1941	Hysterectomy
8066	E,53 Ch , 20x18xI8	Exc.	Bouin	C-P	Transverse	8	(Stain - Haematoxylin Eosin)	1942	Hysterectomy. Ag added to slide 2
8119	E., 5.3 Ch., 32x28x6.5	Exc.	Bouin	C-P	Transverse	8	(Stain - Haematoxylin Eosin)	1943	Hysterectomy
8147	E., 5.2 Ch., 27x21x19	Poor	Formalin	?	?	p	p	1943	Tubal Not cut
8239	E., 4.3	Exc.	Bouin	C-P	Sagittal	8	H. phlox.	1944
8372	E., 5.6	Exc.	Alc.& Bouin	P	Transverse	10	Azan	1946
8581	E., 4.8	Good	Kaiserling	C-P	Sagittal	8	Azan	1948	Most-advanced third
8967	E., 5.7	Exc.	Acetic Zenker	C-P	Transverse	6	(Stain - Haematoxylin Eosin)	1931	Head injured. Univ. Chicago No. H1426
9296	E,4.5	Exc.	C-P	Coronal	8	Azan	1955
9297	E., 4.5	Exc.	C-P	Sagittal	8	Azan	1955
9697	E., 5.5	Bouin	1956	not cut
Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined.

Stage 14

• 

  6830 left

• 

  1380 left

• 

  7333 left

• 

  6502 left

• 

  left

• 

  6502 left

• 

  Template:CE4629 right

• 

  7394 right

• 

  6848 left

• 

  7400 right

• 

  7394 left

• 

  1620 left

• 

  7400 left

Carnegie Collection Embryos - Stage 14
Serial No.	Size (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Year	Notes
4	E.,7	Poor	p	P	Transverse	10	Al. coch.	1892
18	E.,7 Ch., 18x18	Poor	p	P	Transverse	20	Al. coch.	1895
80	E., 5.0 Ch., 24x18x8	Good	Alc.	P	Transverse	20	Al. coch.	1897
187	E.,7 Ch., 35x30x25	Poor	?	P	Sagittal	20	Al. coch.	1902
1208	E.,7 Ch., 22x11Xx1	Poor	?	P	Sagittal	20	Al. coch.	1902
245	E.,6 Ch., 13x12x10	Poor	Formol, Zenker	?	Transverse	5	(Stain - Haematoxylin Eosin)	1904
372	E.,7	Fair	p	P	Transverse	10	H.-Congo red	1902
380	E,6 Ch., 20x20x14	Poor	p	P	Sagittal	20	(Stain - Haematoxylin Eosin)	1906
387	E.,7 Ch., 45x40x50	Good	Formalin	P	Transverse	20	(Stain - Haematoxylin Eosin)	1907
442	E.,6 Ch., 25x20	Poor	Formalin	P	Coronal?	50	Al. coch.	1908
552	E.,6 Ch, 40x28Xx8	Poor	p	P	Sagittal	40	Al. coch.	1911	Possible anencephaly
560	E., 7.0 Ch, 24x24	Poor	Formalin	P	Coronal	40	Al. coch.	1912
676	E., 6.0 Ch, 35x20x17	Good	Carnoy	P	Tr.-Coronal	20	(Stain - Haematoxylin Eosin)	1913	Possible spina bifida
873	E,6.0 Ch., 35x28x16	Poor	Formalin	P	Sagittal	20	Al. coch.	1914
988	E,6.0 Ch., 38x30x23	Good	Formol-corros. acetic	P	Transverse	20	Al. coch.	1914
1380	E , 5.7 Ch, 36x24x24	Exc.	Formalin	P	Coronal	20	Al. coch.	1916
1620	E, 6.6 Ch, 35x30x8	Good	Formalin	P	Sagittal	20	Al. coch.	1916
??	E, 6.68	Fair	?	?	Transverse	6	Al. coch., or. G	1919
2841	E , sy Ch. 35x21	Good	Alc.	P	Transverse	20	(Stain - Haematoxylin Eosin) or.G	1920
3360	E.6.0	Good	Formalin	C	Transverse	20	(Stain - Haematoxylin Eosin) or.G	1920	In myomatous uterus. Advanced.
3805	E., 5.9	Exc.	Bouin	P	Transverse	15	(Stain - Haematoxylin Eosin)	1921	Evans embryo No. 168. Serial bromides only
3960	E., 5.5	Good	Formalin	C-P	Coronal	20	Al. coch.	1922	Blood vessels naturally injected
4154	E, 6.8 Ch., 33x31x20	Poor	Alc.	C-P	Transverse	8	(Stain - Haematoxylin Eosin)	1923
4245-6	E., 7.0	Good	Formalin	P	Transverse	15	Al. coch.	1923	Univ. Pennsylvania No. 40. Ag added
4692	E., 6.5 Ch., 32x23	Good	Formalin	C-P	Sagittal	10	(Stain - Haematoxylin Eosin)	1924
4672	E,8.2 Ch., 40X34X25	Good	Formalin	P	Transverse	20	Al. coch.	1924	Advanced
4805	E., 7.3 Ch., 15X8X9	Good	Formalin	C-P	Transverse	10	(Stain - Haematoxylin Eosin)	1924	Tubal
5437	E., 7.0	Good	Formalin	C-P	Transverse	8	(Stain - Haematoxylin Eosin)	1927	Advanced
5654	E., 5.0 Ch., 30x23x17	Good	Formalin	P	Transverse	10	Al. coch., eosin	1928	Less advanced
5787	E., 6.8 Ch., 32x30x23	Good	Formalin	P	Sagittal	10	Al. coch., eosin	1928
6428	E., 7.0 Ch., 30X28X25	Good	Formalin	C-P	Coronal	6, 10	Al. coch.	1931	Advanced
6500	E, 4.9*	Good	Souza?	C-P	Sagittal	10	Al. coch.	1931	E. Leitz, Berlin
6502	E., 6.7*	Exc.	Souza?	C-P	Transverse	5, 10	(Stain - Haematoxylin Eosin)	1931	E. Leitz, Berlin. Ag added to slides 1-25
6503	E., 6.3*	Exc.	Souza?	C-P	Coronal	10	Al. coch.	1931	E. Leitz, Berlin
6739	E.,8	Poor	Formalin	C-P	Sagittal	20	(Stain - Haematoxylin Eosin)	1933
6830	E,5.5 Ch., 47x23x15	Exc.	Formalin	C-P	Coronal	8	(Stain - Haematoxylin Eosin)	1933
6848	E., 7.8	Good	Formalin	C-P	Coronal	10	(Stain - Haematoxylin Eosin)	1934	Tubal
7324	E, 6.6 Ch., 17x13x10	Good	Formalin	C-P	Transverse	8	(Stain - Haematoxylin Eosin)	1936	Low implantation
7333	E, 6.3	Good	Formalin	C-P	Transverse	8	(Stain - Haematoxylin Eosin)	1936
7394	E, 7.2 Ch., 45x20x20	Exc.	Formalin	C-P	Transverse	8	(Stain - Haematoxylin Eosin)	1937
7400	E, 6.3 Ch., 35x25x20	Good	Formalin	C-P	Coronal	10	(Stain - Haematoxylin Eosin)	1937
7522	E., 7.7 Ch., 33x16x16	Good	Formalin	C-P	Transverse	8	(Stain - Haematoxylin Eosin)	1938	Natural blood injection
7598	E., 7.0 Ch., 30x30x25	Poor	Alc.	C-P	Transverse	10	(Stain - Haematoxylin Eosin)	1938	Macerated
7667	E., 5 Ch., 16x14x12	Fair	Formalin	P	Transverse	8	(Stain - Haematoxylin Eosin), phlox.	1939
7829	E., 7.0	Exc.	Bouin	C-P	Transverse	8	(Stain - Haematoxylin Eosin)	1940	Advanced
7870	E,7.2 Ch., 25x20x13	Exc.	Bouin	C-P	Transverse	8	(Stain - Haematoxylin Eosin)	1941	On borderline of next stage. Ag added
8141	E,7.3 Ch.,33x28	Exc.	C-P	Coronal	8	(Stain - Haematoxylin Eosin)	1943	Shrinkage cracks in brain
8306	E.5.3 Ch., 27	Exc.	Bouin	C-P	Transverse	10, 20	(Stain - Haematoxylin Eosin), phlox.	1945
8308	E, 5.85 Ch., 27x18x18	Exc.	Formol & Bouin	C-P	Sagittal	8	Azan	1945
8314	E,8 Ch.,23x22	Exc.	Formol	C-P	Transverse	8	Azan	1945
8357	E., 6.5	Good	Formol	C-P	Sagittal	8	Azan	1946
8552	E,6.5	Exc.	Alc. & Bouin	C-P	Transverse	8	Azan	1947
8999	E,6 Ch.,16x15	Exc.	Alc. & Bouin	C-P	Sagittal	8	Azan	1952
9695	E,8.5	?						1955	Not cut
Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined.

Stage 15

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Carnegie Collection Embryos - Stage 15
Serial No.	Size (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Year	Notes
2	E., 7.0 Ch., 25x25x25	Good	Alc.	P	Transverse	15	Al. carm.	1888	Least-advanced third
88	E.,8 Ch., 30x28x15	Poor	Alc.	P	Coronal	P	Al. coch.	1897
113	E.,8 241 E,6.0	Poor	P	p	Sagittal	10	Borax carmine	?
241	E,6.0	Good	Formalin	P	Transverse	10	H. & Congo red	1904
371	E,6.6	Good	Formalin	P	Sagittal	10	Al. coch.	1913	Shrunken and cracked
389	E., 9	Poor	p	p	Sagittal	20	(Stain - Haematoxylin Eosin)	1907	Tubal
721	E., 9.0 Ch., 30x20x10	Exc.	Zenker formol	P	Transverse	15	(Stain - Haematoxylin Eosin)	1913	Median in group
810	E., 7.0 Ch, 30x25x15	Good	Alc.	P	Sagittal	20	Al. coch	1913
855	E.,7.5	Poor	Formalin	P	Transverse	100	Al. coch.	1914	Pathological between limbs
1006	E,9.0 Ch., 37x26x22	Poor	Formalin	P	Coronal	20	(Stain - Haematoxylin Eosin) or. G.	1914	Operative. Most-advanced third
1091	E,7.2 Ch., 28x26x20	Poor	P	P	Coronal	20	Al. coch.	1915	Macerated
1354	E,7.8 Ch, 35x30x25	Good	Formalin	P	Sagittal	20	Al. coch.	1916	Least-advanced third
1767	E , 11.0 Ch, 41x23x5	Good	Formalin	P	Sagittal	40	(Stain - Haematoxylin Eosin) or. G.	1917	Most-advanced third
2743	E., 7.2 Ch., 19xl8x14	Poor	Formalin	P	Transverse	20	Al. coch.	1919	Macerated. Least-advanced third
3216	E, 6.5 Ch, 30x30x5	Good	Formalin	P	Transverse	20	Al. coch.	1920	Hysterectomy. Least-advanced third
3385	E,83 Ch., 25x20x16	Exc.	Corros. acetic	P	Trans.	20	(Stain - Haematoxylin Eosin) or. G.	1921	Some sections lost. Most-advanced third. Ag added
3441	E,8.0 Ch., 25x24x20	Good	Formalin	P	Sag.	10	Al. coch.	1921
3512	E,8,5 Ch., 33x28x25	Good	Formalin	P	Trans.	10	Al. coch.	1921
3952	E,6,7 Ch., 30x25x15	Good	Formalin	P	Cor.	15	Al. coch.	1922	Median in group
4602	E,9.3 Ch,, 33x30x26	Good	Formalin	P	Sag.	15	Al. coch.	1924	Medical abortion
4782	E,9.0 Ch., I4xl3x11	Poor	Formalin	P	Cor.	20	Al. coch.	1924
5772	E, 8	Poor	?	P	Cor.	15	Al. coch. eosin	1928
Template:CE5?92	E, 3	Good	Corros. acetic	C-P	Cor.	10	Al. coch. phlox	1929	Transitional to next stage
6223	E ?	Poor	Alc.	C-P	Sag.	8	Or. G.	1930	Fragmented sections. Not saved
6504
6506
6508
6595
????
Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) ? - unknown or not determined.

Stage 16

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Carnegie Collection Embryos - Stage 16
Serial No.	Size (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Year	Notes
163	E., 9.0 Ch., 35x35x20	Good	Formol	P	Transverse	20	Al. coch.	1899	Used by Bardeen and Lewis
221	E., 7.5 Ch., 40x33x33	Poor	Formalin	P	Sagittal	20	Al. coch.	1903	Macerated
383	E., 7.0 Ch., 15x15x15	Poor	Formalin	P	Transverse	10	Al. carm., H. & Congo red	1904
397	E., 8.0 Ch., 15x15x15	Poor	Formalin	P	Transverse	10	(Stain - Haematoxylin Eosin)	1907
422	E., 9.0 Ch., 30x30x30	Poor	Alc.	P	Transverse	40	Al. coch.	1910	Tubal. Partly macerated
559	E., 8.6 Ch., 20x15x12	Good	Formalin	P	Transverse	20	H. & Congo red	1911	Cyclopia. Formerly listed as stage 17
589	E., 11 Ch., 30x13x13	Poor	p	P	Sagittal	50	Al. coch.	1912
617	E., 7.0 Ch., 18x14x12	Good	Formalin	P	Transverse	15	Al. coch.	1912	Median in group
636	E., 10 Ch.,28x28x22	Poor	Formalin	P	Transverse	50	Al. coch.	1913	Macerated
651f	E., 7 Ch., 25x20x15	Poor	p	p	p	p	p	1913	Spina bifida
675	E., 10 Ch., 50x30x25	Poor	Formalin	P	Sagittal	100	Carmine	1915	Abnormal head and limbs
792	E., 8.0 Ch., 40x30x30	Good	Formalin	P	Transverse	20	Al. coch.	1913	Advanced
887	E, 9.0 Ch., 31x28x17	Good	Formalin	P	Transverse	40	Al. coch.	1914	Near next stage
1121	E., 11.8	Good	Corros. acetic	P	Coronal	40	Al. coch.	1915	Operative. Median in group
1197	E., 10.0 Ch., 23x19x15	Good	Formalin	C	Sagittal	20	(Stain - Haematoxylin Eosin), or. G.	1915	Advanced
1544	E., 7.2	Good	Zenker	P	Sagittal	20	Al. coch.	1916	Tubal. Mechanical injury
1836	E., 11.0	Good	Formalin	P	Transverse	20	(Stain - Haematoxylin Eosin)	1917	Most-advanced third
4677	E., 9.5 Ch., 48x36x30	Good	Formalin	P	Transverse	10	Al. coch.	1924	Median in group
5515	E., 12.0 Ch., 47x37x25	Good	Formalin	C-P	Transverse	10	(Stain - Haematoxylin Eosin)	1927	Near next stage
6054	E., 7,0 Ch., 21x17x12	Good	Formalin	C-P	Transverse	8	(Stain - Haematoxylin Eosin)	1930	Least-advanced third
6507	E., 9.0*	Excellent	Corros. acetic	C-P	Coronal	10 & 8	Al. coch.	p	Middle or most-advanced third
6509	E. 8.1*	Excellent	Corros. acetic	C-P	Coronal	10	Al. coch.	p	Least-adianced or middle third
6510	E., 10.1*	Excellent	Corros. acetic	C-P	Coronal	10	Al. coch.	p	Close to No. 6507. Ag added
6511	E, 8.1*	Good	Corros. acetic	C-P	Sagittal	10	Al. coch., iron H.	p	Surface injured by fixative. Most-advanced third
6512	E., 7.0*	Excellent	Corros. acetic	C-P	Transverse	10	Al. coch.	p	Least-advanced third. Borderline
6513	E., 7.2*	Good	Corros. acetic	C-P	Coronal	10	Al. coch.	p	Surface injured by fixative. Least advanced in group
6514	E, 9 0*	Poor	Corros. acetic	C-P	Sagittal	10	Al. coch.	p	Most-advanced third
6516	E., 10 5*	Good	Corros acetic	C-P	Sagittal	8	Al. coch.	p	Most-advanced third. Double left kidney and ureter
6517	E., 10.5*	Excellent	Corros. acetic	C-P	Transverse	8	Al. coch.	?	Close to No. 6516
6686	E., 11.0 Ch., l7x17xP	Poor	Formalin	C-P	Coronal	20	Al. coch.	1933	Tubal. Partly macerated
6750	E., 10.0	Good	Formalin	C-P	Transverse	10	H. & phlox.	1933	Tubal. Advanced
6909	E., 11.0	Good	Bouin	C-P	Coronal	10	(Stain - Haematoxylin Eosin)	1934	Tubal. Advanced
6931	E., 8.8 Ch., 3""x 33x16	Good	Formalin	C-P	Coronal	10	Al coch., phlox.	1934	Least-advanced third. Type specimen
6950	E.. 9 0 Ch., 3lx20x18	Good	Formalin	C-P	Transverse	10	(Stain - Haematoxylin Eosin)	1934	Tubal. Partly fragmented
7?15	E., 9.7	Exc	Bouin	C-P	Coronal	10	H. & phlox	1935	Operative. Less advanced
7629	E., 11.5 Ch., 31x31	Good	Formalin	C-P	Coronal	10	Al. coch., phlox	1939	Hysterectomy. Most advanced in group
7804	E., 9.5 Ch., 26x21x16	Good	Formalin	C-P	Transverse	10	(Stain - Haematoxylin Eosin)	1940	Least-advanced third
7897	E., 12.2 Ch., 31x24x23	Good	Formalin	C-P	Transverse	10	(Stain - Haematoxylin Eosin)	1941	Tubal. Advanced
8098	E., 10.0 Ch., 30	Good	Formalin	C-P	Coronal	6	(Stain - Haematoxylin Eosin)	1942	Tubal. Median in group
8112	E., 10.9	Excellent	Bouin	C-P	Coronal	8	(Stain - Haematoxylin Eosin)	1943	Most-advanced third
8179	E., 11.9 Ch., 23x18x17	Good	Formalin	C-P	Coronal	10	(Stain - Haematoxylin Eosin)	1943	Tubal
8436	E., 10.9 Ch., 13x15x1?	Good	Formalin	P	Coronal	10	Azan	1946	Advanced
8692	E., 10	Good	Bouin	P	Transverse	10	(Stain - Haematoxylin Eosin)	1949	Rubella. Medical abortion. Mechanically damaged
8697	E., 11.3	Poor	Formalin	C-P	Transverse	10	(Stain - Haematoxylin Eosin)	1949	Perhaps stage 17
8773	E, 11	Excellent	Bouin	P	Coronal	10	Azan	1950
8971	E., 10 Ch., 20.5x14.5x13.7	Poor	Formalin		Transverse	15	(Stain - Haematoxylin Eosin)	1932	Synophthalmia. Univ. Chicago No. H 1439
Template:CE9055	E., ca. 10	Excellent	Bouin	P	Transverse	20	Azan & Ag	1953	Damaged
9229	E, 9.5	Excellent	Formalin	P	Transverse	6	Ag & (Stain - Haematoxylin Eosin)	1954	Stage 15, 16, or 17? Mislaid
Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin Bouin solution) Stain - ? - unknown or not determined.

Stage 17

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Carnegie Collection Embryos - Stage 17
Serial No.	Size (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Year	Notes
353	E, 11.0 Ch., 40x35x20	Good	Formalin	P	Coronal	10	(Stain - Haematoxylin Eosin)	1906	Very advanced
485	E, 13.0 Ch., 33x25	Exc.	Formalin	P	Coronal	40	Al. coch.	1911	Injected (India ink)
544	E., 11.5 Ch., 30	Good	Zenker-Formol	P	Sagittal	40	Al. coch.	1911	Operative Injected (India ink)
562	E, 13.0 Ch., 28x17x17	Poor	Formalin	P	Sagittal	100	Al. coch.	1912	Advanced
623	E 10.1	Good	Alc.	P	Transverse	20	H. & Congo red	1912	Operative. Median in group
695	E, 13.5 Ch., 40x40x17	Poor	Formalin	P	Transverse	10	H. & Congo red	1913	Macerated
916	E, 11.0 Ch, 30x30x16	Good	Bouin	C	Transverse	40	H.&E, or. G.	1915	Most-advanced third
940	E, 14.0 Ch, 28x23x21	Good	Formalin	C	Transverse	40	H.&E, or. G.	1914	Advanced
1232	E, 14.5 Ch., 35x35x30	Poor	Formalin	P	Coronal	40	Al. coch.	1915	Close to No.1267A
1267A	E-a 145 Ch., 35x30x26 40	Good	Formalin	C	Sagittal	20	(Stain - Haematoxylin Eosin). or. G	1915	Excellent CN.S.
1267B ??	E, 125	Good	Formalin	p	Sagittal	20	(Stain - Haematoxylin Eosin)	1917	Tubal
5642	E, 11.5 Ch.. 33x30x17	Good	Formalin	p	Transverse	15	Al. coch.	1928	Right upper limb injured
5893	E.. 13.2	Good	Formalin	C-P	Transverse	20	Al. coch.	1929	Most advanced in group
6258	E. 14.0 Ch 48x35x25	Good	Formalin	C-P	Transverse	10	(Stain - Haematoxylin Eosin)	1930	Median in group
6519	E.. 10.8*	Exc	Corrov acetic	C-P	Sagittal	8	Al. coch.	?	Least-advanced or middle third
6520	E., 14.2*	Exc.	Corros. acetic	C-P	Transverse	10	Al. coch.	P	Median in group. Ag added to slides 1-25
6521	E., 13.2*	Exc.	Corros. acetic	C-P	Transverse	8-18	Al. coch.	?	Sections vary in thinness
6631	E., 13.0	Good	Formalin	C-P	Coronal	10	(Stain - Haematoxylin Eosin)	1932	Tubal. Advanced
6742	E, 11.0 Ch.,50x40x15	Good	Formalin	C-P	Transverse	12	H. & phlox.	1933	Good primary germ cells
6758	E., 12.8	Good	Formalin	C-P	Transverse	10	H. & phlox.	1933	Least—advanced third
7317	E, 10.0*	Good		P	Coronal	10	(Stain - Haematoxylin Eosin)	1936	His embryo “Ru." Every third section
7436	E., 13.0	Good	Formalin	C-P	Coronal	30	Al. coch.	1937	Most-advanced third
8101	E., 13.0	Exc.	Bouin	C-P	Transverse	10	(Stain - Haematoxylin Eosin)	1943	Operative
8118	E., 12.6	Exc.	Bouin	C-P	Coronal	10	(Stain - Haematoxylin Eosin)	1943	Middle third
8253	E., 11.2 Ch.,30x20x10	Good	Bouin	C-P	Coronal	10	Al. coch., phlox.	1944	Operative. Least advanced in group
8789	E., 11.7	Exc.	Bouin	C-P	Sagittal	10	Azan	1950
8969	E., 11.2	Exc.	?	p	Transverse	15	Azan	1919	Univ. Chicago No. H566
8998	E., 11.0	Exc.	?	C-P	Coronal	10	Azan	1952
9100	E., 12.0 Ch., 12x13x10	Exc.	Formol-chrom. subl.	C-P	Sagittal	10	Azan	1933	Univ. Chicago No. H1475
9282	E, 12.0 Ch., 16	Good	Ale.	p	Transverse	15	Ag	1955	Mislaid
Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined.

Stage 18

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Carnegie Collection Embryos - Stage 18
Serial No.	Size (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Semi. ducts	P.-M. duct (mm)	Year	Notes
109	E., 12.0* Ch.,30	Poor	Alc.	P	Transverse	20	Al. coch.	1	0.4	1897	Tubal Least—advanced third
144	E., 16.0* Ch, 40x30x30	Good	Formalin	P	Sagittal	40	Al. eoch.	3	0.85	1899	Most—advanced third
175	E., 13.0 Ch, 30x25x25	Poor	Alc.	P	Transverse	20	Al. coch.	2	0.6	1900	Tubal Partly macerated
296	E., 17.0	Poor	Ale.	P	Coronal	20	Various	3	0.85	1905	Most—advanced third
317	E., 16.0	Good	Formalin	P	Coronal	20	(Stain - Haematoxylin Eosin) or. G.	2	0.7	1905	Middle third
351	E.,14.0*	Good	Formalin	P	Coronal	250	Slightly carmine—	2	038	1904	Injected (Berlin blue)
406	E., 16.0 Ch., 40x40x40	Good	Formalin	P	Sagittal	20	(Stain - Haematoxylin Eosin)	3	0.7	1907	Operative. Most—advanced third
423	E., 15.2	Good	Formol—Zenker	P	Transverse	50	Carmine	3	0.85	1904
424	E., 172	Good	Formalin	P	Transverse	50	Carmine	3	10	1904	Double infection. Advanced
492	E, 16.8 Ch, 40 x 40	Exc.	Zenker	P	Coronal	40	Al. coch. v	3	0.7	1911	Injected (India ink)
511	E., 160* Ch., 3?* 32x32	Good	Ale.	P	Sagittal	40	Al. coch.	3	1.1	1911	Head injured. Most advanced in group
670	E, 12.5	Poor	Ale.	P	Sagittal	50	(Stain - Haematoxylin Eosin)	3	10	1913	Tubal Advanced
719	E, 15.0 Ch, 50x50x50	Good	Formalin	P	Trans	40	Al. coch.	2	0.6	1913	Median in group
733	E., Ch., 4Sx40x2S	ISO Poor	Formalin	P	Sagittal	50	Al. coch.	2	0.6	1913	Median in group
841	E. 15.0 Ch., 18 x 16x9	Good	Formalin	P	Coronal & Trans, 20	10	(Stain - Haematoxylin Eosin), carmine	2	0.32	1914	Operative. Head cut separately
899	E, 160* Ch. 50 x 18 x IS	Good	Bouin	P	Sagittal	S0	Al. coch.	3	0,65	1914	Tubal Head injured
991	E. l?.0	Good	Formalin	P	Sag	so	R, V, Gieson	3	0.9	1914	Advanced
1909	E., 14.6	Good	Formalin	P	Coronal	20	Al. coch,or. G.	1	0.3	1917	Less advanced
2673	E.,15.5	Good	Formalin	P	Transverse	40	Al. coch.	2	0.52	1919	Median in group
4430	E., 14.0 Ch, 51 x40x21	Exc.	Corros. acetic	P	Transverse	15	Al. coch,or. G.	3	0.9	1923	Most—advanced third
5542B	E., 16.0 Ch, 37x32x25	Good	Formalin	P	Transverse	40	Al. coch.	2	0.7	1927	Other twin abnormal
5747	E, 15.2 Ch, 32x27x25	Poor	Alc.—formol	P	Sagittal	25	Al. coch.	2	0.25	1928	Least—advanced or middle third
5935A	E, 13.5 Ch, 40x30x30	Good	Formalin	P	Coronal	40	Al. coch.	1	0.38	1929	Other twin stunted
6522	E, 13.2*	Good	Corros. acetic	C—P	Coronal	10	Al. coch.	3	0.8	7	Middle or most—advanced third
6524	E, 11.7*	Exc.	Corros. acetic	C—P	Transverse	10	Al. coch.	1	0.4	?	Least—advanced third
6525	E, 13.8*	Exc.	Corros. acetic	C—P	Sagittal	8	Al. coch.	2	0.42	?	Weak staining
6527	E, 14.4*	Exc.	Corros. acetic	C—P	Transverse	15	Al. coch.	2	0.67	?	Mechanical damage
6528	E, 13.4*	Exc.	Corros. acetic	C—P	Coronal	8	Al. coch.	1	0.33	?	Least—advanced third
6529	E, 15.6*	Good	Corros. acetic	C—P	Coronal	10	Al. coch.	2	0.4 .5		Middle third
6533	E, 12.5*	Good	Corros. acetic	C—P	Sagittal	6, 8, 10	Al. coch.	2	0.45	?	Middle third
6551	E, 18.0	Poor	Formalin	p	Coronal	40	(Stain - Haematoxylin Eosin)	3	0.8	1932	Tubal
7707	E, 14.5 Ch ,37x32	Exc.	Bouin	C—P	Transverse	10	(Stain - Haematoxylin Eosin), phlox.	2	0.54	1939	Operative. Middle third
8097	E, 15.5 Ch, 37x25x21	Good	Formalin	C—P	Transverse	10	(Stain - Haematoxylin Eosin)	1	0.19	1942	Least advanced in group
8172	E, 16.5	Exc.	Bouin	C—P	Transverse	20	(Stain - Haematoxylin Eosin)	3	0.58	1943	Operative. Very advanced
8235	E, 14.0*	Good	Bouin	C—P Sagittal	10	(Stain - Haematoxylin Eosin)	Mallory	2	0.25	1944	Tubal
8355	E, 15.0 Ch, 23	Exc.	Formalin	C—P	Coronal	10	Azan			1946	Tubal. Duplicated spinal cord caudally
8812	E, 12_9	Exc	Formalin	C—P	Transverse	10	(Stain - Haematoxylin Eosin)			1950	Rubella. Medical abortion. Midbrain punctured
8945	E, 13.9	Good	Zenker	p	Transverse	8	Borax, carm.			1952	Univ. Chicago No. H 1254
9107	E, 17.0 Ch, 38x28x22	Good	Bouin	p	Transverse	15	Borax, carm.			1918	Univ. Chicago No. H 516
9247	E, 15.0	Exc.	Bouin	C—P	Sagittal	8	Azan			1954	Tubal
Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined.

Stage 19

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Carnegie Collection - Stage 19
Serial No.	Size (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Score	Sex	Year	Notes
17	E, 18 Ch, 40x30x20	Poor	Alc.	P		50, 100	Al. carm.	16.5	Male	1894
43	E, 16	Good	Alc.	P		50	Al. coch.	10	Male	1894
293	E, 19	Poor	Ale.	P	Sagittal	50	Coch.	16.5	S	1905
390	E, 19	Good	Formol?	P	Sagittal	20,	(Stain - Haematoxylin Eosin)	11.5	Male	1906	Tubal Injected 50
409	E.18 Ch, 50x40x40	Good	Formalin	P	Transverse	20	Copper, iron H. & erythrosin	14.5	Male	1907
432	E..18.5 Ch , 45x35x20	Good	Formalin	P	Sagittal	20	H. & Congo red	13.5	Male	1910	Tubal
576	E. 17 Ch, 60x40	Good	Formalin	P	Sagittal	15, 20	(Stain - Haematoxylin Eosin)	14.5	d	1912	Tubal
626	E., 21.5 Ch., 40x30x21	Good	Formalin	P	Transverse	100	Al. coch.	14_5	6	1913
6??8	E, 20 Ch, ca. 30	Poor	Formalin	P	Sagittal	50	Al. coch.	12	9	1913	Head damaged
709	E, 19 Ch. 40x35x25	Poor	Alc.	P	Coronal	40	Al. coch, Lyons blue	15	49	1913
837	E. 21 Ch. 65x45x	Good	Formalin	P	Sagittal	40	Al. coch.	14.5	P	1914
1324	E., 18 50x30x18	Good	Formalin	C	Coronal	40	(Stain - Haematoxylin Eosin), aur, or. G	125	79	1915
1332	E., 19 Ch., 40x43x22	Poor	Formalin	C	Coronal	40	(Stain - Haematoxylin Eosin) aur, or. G.	15	Male	1915
1390	E., 18 Ch, 40x38x15	Good	Formalin	P	Sagittal	20	Al. coch.	10_5	Male	1915	Tubal
1534	E., 13 Ch.,35x31x25	Poor	Formalin	P	53%	50	Al. coch.	13.5	F	1916	Protruding midbrain
2114	E., 19.3 Ch., 49x42x33	Good	Formol	P	Transverse	40	A1. coch.	12	M	1918
4405	E., 15.5	Good	Formalin	P	Transverse	10	Coch, Mallory	13.5	<3	1923	Midbrain injured
4501	E, 18	Exc.	Bouin	P	Transverse	15	Coch, or. G.	14.6		1924	Cystic left kidney
5609	E., 18	Exc.	Formalin	P	Coronal	25	A1. coch.	13.5	Male
6150	E., 17 Ch., 40x39x30	Good	Bouin	C-P	Transverse	15	(Stain - Haematoxylin Eosin)	16.5	Male	1930	Tubal
6824	E., 18.5 Ch., 45x40x25	Good	Formalin	C-P	Sagittal	12	(Stain - Haematoxylin Eosin)	14.5	Female	1933
7900	E., 16.5	Good	Bouin	C-P	Sagittal	20	(Stain - Haematoxylin Eosin), phlox.	11.5	. .	1941	Tubal
8092	E., 16.3 Ch., 52 x 47	Exc.	Bouin	C-P	Transverse	20	(Stain - Haematoxylin Eosin), phlox.	13	Male	1942
8913	E.,? Ch, 34	Poor	Formalin	p	Transverse	10	Alan .	7		1951	rubella. Medical abortion. Isolated head damaged
8965	E, 19.1 Ch, 42x32x19	Good	Formol—Zenker	C-P	Transverse	10	Borax, carm, or. G.			1952	Univ. Chicago No. H 173
9097	E, 21	Exc.	Formol—glucose	C-P	Coronal	10	Azan ? .	?		1930	Univ. Chicago No H 1380
9113	E, 185 Ch, 24	Exc.	Formalin	C-P	Transverse	10	Alan > 6			1953	Rubella. Medical abortion
9325	E, 17.0 Ch, 32x28x20	Good	Formalin	—acetic p	Transverse	15& 8-10	Azan & Ag	?	-	1955	Tubal
Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined.

Stage 20

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Carnegie Collection - Stage 20
Serial No.	Size (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Point Score	Sex	Year	Notes
240	E, 20 Ch, 50x40x30	Poor	Formalin	P	Coronal	20	Iron H.	27	d	19??
256	E, 21	poor	Alc	p	Sagittal	25	Coch.	23	M	1904	Tubal. Partial anencephaly
353	E, 20	Poor	Alc	P	Sagittal	20	H. & Congo red	22,5	M	1906
431	E, 19 Ch, 30x25x25	Good	Formalin	P	Sagittal	20	H. & Congo red	25,5	M	1908	Tubal
437	E, 23 Ch., 80x60x50	Poor	Formalin	P	Sagittal	50	Coch	24	M	193?
453	E, 23 Ch, 60x40x30	Poor	Formalin	P	Sagittal	20	H. & Congo red	23-5	?	1910	Injected
460	E, 21	Exc.	Bichlor. acetic	P	Transverse	40	(Stain - Haematoxylin Eosin), coch,	24.5	M	1910	injected
462	E, 20 Ch, 50x40x30	Exc.	Formalin	P	Transverse	40	Al, coch	23.5	F	1910
635B	E, 22	Poor	Alc	P	Transverse	50	Al, coch	26.5	M	1913
657	E, 25 Ch, 35x20x15	Poor	Formalin	C	Sagittal	40	Al, coch	26.5	M	1913	Tubal
966	E, 23 Ch, 51x38x13	Exc	Bichlor. acetic	P	Coronal	40	(Stain - Haematoxylin Eosin), aur, or. G	25	M	1911	Tubal
1134B	E, 23	Poor	Formalin	p	Sagittal	100	Al. coch.,	22	-	1915
1266	E, 23.1	Poor	Formalin	C-P	Sagittal	25	Al. coch., (Stain - Haematoxylin Eosin) aur, or G	20.5	F	191?
2393	Ch, 61.5x50x35	Poor
3527	E., 22 Ch. 32x30x10	Good	Formalin	P	Sagittal	25	Al. coch.,	28	?	1921
4059	E, 21.6	Good	Formalin	P	Coronal	15	Al. coch., Mallory	29.5	$	1922
4148	E, 21 Ch. 45x34x30	Good	Formalin	p	Coronal	15	A1. coch., Mallory	19	?	1922
4361	E, 22 Ch., 52x42x23	poor	Formalin	9	Transverse	20	Coch.	24	8	1923
6202	E, 21 Ch., 35x35x22	Exc	Bouin	P	Sagittal	20	(Stain - Haematoxylin Eosin)	20.5	8	1930	Tubal
6426	E 21.5	Good	Formalin	C—P	Transverse	20	(Stain - Haematoxylin Eosin)	21	3	1931
7274	E, 18.5 Ch., 48x44x35	Exc	Bouin	C—P	Transverse	20	(Stain - Haematoxylin Eosin), phlox.	20	M	1936
7906	E19.5	Exc	Bouin	C—P	Coronal	20	(Stain - Haematoxylin Eosin)	22	8	1941	Left renal agenesis
8517	E., 20.8	Exc.	Bouin	C—P	Coronal	20	(Stain - Haematoxylin Eosin)	24	8	1943
8226	E, 18.0	Exc.	Bouin	C—P	Sagittal	10	Alan	?	3	1944
Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined.

Stage 21

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  Embryo No. 7392 (right)

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  Embryo No. 7392 (front)

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  Embryo No. 7392 (left)

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  Embryo No. 8553 (right)

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  Embryo No. 8553 (front)

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  Embryo No. 8553 (left)

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  Embryo No. 4090 (right)

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  Embryo No. 4090 (front)

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  Embryo No. 4090 (left)

Carnegie Collection - Stage 21
Serial No.	Size (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Point Score	Sex	Year	Notes
22	E, 20 Ch, 35x30x30	Good	Alc.	P	Transverse	50	Al. coch.	34.5	Female	1895
57	E, 23 Ch., ca. 30	Poor	Alc.	P	Sagittal	50	Al. coch.	36	Male	1896
128	E, 20 Ch., 50x43	Good	Formalin	P	Coronal	50	Al. coch.	33	Female	1898
229	E, 19	Poor	Alc.	P	Sagittal	50	Al. coch.	33	Female	1903
349	E, 24	Good	Zenker	C	Coronal	250	Unstained	36	?	1905	Double vascular injection
455	E, 24 Ch., 42x34x20	Good	Alc.	P	Transverse	30	(Stain - Haematoxylin Eosin)	36.5	Male	1910
632	E, 24 Ch., 60x50x30	Good	Bichlor. acetic	P	Sagittal	40, 100, 250	Al. coch.	33	Female	1913	Injected
903C	E, 23.5	Good	Formalin	P	Transverse	40	Al. coch.	38.5	Female	1914
1008	E, 26,4	Good	Formalin	P	Sagittal	40	Al. coch.	39	??	1914
1358F	E, 23	Good	Formalin	P	Sagittal	40	Al. coch.	37.5	Female	1916
2937	E,, 24.2	Good	Bouin	P	Transverse	50	(Stain - Haematoxylin Eosin) aur., or. G.	39	Female	1920
3167	E., 24.5 Ch., 60x50x40	Poor	Bichlor, acetic, formol	P	Transverse	20	Al. coch.	32	Male	1920
4090	E, 22.2 Ch.. 66x46x30	Good	Formalin	P	Transverse	40	Al. coch.	30	Female	1922
4160	E,25	Poor	Formalin	P	Sagittal	25	(Stain - Haematoxylin Eosin)	39	Male	1923	Tubal
4960	E.22 Ch,, 47x42x28	Good	Formalin	P	Transverse	15	Al. coch., Mallory	31.5	Female	1925
5??6	E. 215	Good	Formalin	P	Sagittal	20	(Stain - Haematoxylin Eosin)	34	Female	1927
6531	E,22	Poor	Glacial acetic,	C-P	Transverse	10	(Stain - Haematoxylin Eosin)	31.5	Female	1931	Leitz Collection
7254	E,225	Exc	Bouin	C-P	Transverse	20	(Stain - Haematoxylin Eosin)	33.5	Male	1936
7592	E,22->	Exc.	Bouin	C-P	Transverse	20	(Stain - Haematoxylin Eosin)	36	Female	1937
7864	E., 24	Exc,	Formalin	C-P	Frontal	20	(Stain - Haematoxylin Eosin)	32.5	Male	1941
8553	E., 22	Exc	Bouin	C-P	Transverse	12	(Stain - Haematoxylin Eosin)	38	Female	1947
9614	E,,22 5	Exc	Bouin	P	Coronal	10 &15	Azan	?	?	1958	Rubella. Hysterectomy
Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined.

Stage 22

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Carnegie Collection - Stage 22
Serial No.	Size (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Point Score	Sex	Year	Notes
392	E., 23 Ch., 45x45x25	Poor	...	P	Sagittal	50	Al. coch.	42	Female	1907	Brödel Collection. Injected
405	E., 26	Good	Formalin	C	Sagittal	40	Carmine	42.5	Male	1907
464	E.,26 Ch., 45x40x30	Good	Formol? alc?	P	Sag.	100	Al. coch.	44.5	Male	1910
584A	E.,25 Ch., 50x42x40	Poor	Formalin	P	Sagittal	50	Al. coch.	41	?	1913
630	E., 25	Poor	Formalin	P	Transverse	100	Al. coch.	46	Male	1913
840	E, 24.8	Good	Formalin	P	Transverse	50	Al. coch.	44.5	Female	1914
875	E, 27 Ch., 40x28x22	Good	Formalin	P	Sagittal	40	Al. coch.	45	Male	1914
895	E., 26 Ch., 67x62x54	Good	Formalin	P	Transverse	25	Al. coch.	46.5	Female	1914
1315	E.,25	Good	Formalin	P	Sagittal	50	Al. coch.	40.5	Female	1915	Spina bifida and anencephaly
1458	E., 27.5 Ch, 45x45x30	Exc.	Formalin	C	Sagittal	50	(Stain - Haematoxylin Eosin) aur, or. G.	45.5	Male	1916
1894	E, 24.6	Good	Formalin	c	Sagittal	40,80	(Stain - Haematoxylin Eosin) aur, or. G.	41	Female	1917
2206	E, 27 Ch, 50x30x18	Poor	Formalin	p	Transverse	40	(Stain - Haematoxylin Eosin)	44.5	Male	1918
3681	E, 26.3 Ch, 36x36x34	Good	Formalin	p	Transverse	25	Al. coch.	44.5	Male	1921
4304	E,25 Ch, 66x45x45	Good	Bouin	p	Transverse	20	(Stain - Haematoxylin Eosin)	44.5	Female	1923	Injected
4339	E, 24.5	Good	Formalin	p	Transverse	15	Al. coch, Mallory	46,5	Female	1923
4476	E., 26.2	Good	Bouin	p	Transverse	40	(Stain - Haematoxylin Eosin)	46	Female	1923	Tubal
4638	E, 23.4	Exc.	Bouin	p	Transverse	15,20	Al. coch, or. G.	41.5	Male	1924
6701	E, 24	Poor	Formalin	p	Coronal	20	(Stain - Haematoxylin Eosin)	41	Female	1933
6832	E, 25.8	Exc.	Bouin	C-P	Coronal	20	(Stain - Haematoxylin Eosin)	42	Female	1934
8394	E, 25.3 Ch, 48x50x34	Exc.	Bouin	C-P	Transverse	20	(Stain - Haematoxylin Eosin), Masson	44.5	Female	1946
8948	E, 26.7 Ch, 61x51x50	Poor	Formol-Zenker	p	Transverse	15	Ag	?	?	1952
Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined.

Stage 23

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Carnegie Collection - Stage 23
Serial No.	Size (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Point Score	Sex	Year	Notes
45	E,28 Ch, 40x35x20	Poor	?	P	Coronal/Transverse	50	Al. coch.	51.5	Female	1895
75	E,30	Good	Alc.	P	Sagittal	50	Coch.	57	Male	1897
86	E,30	Good	?	?	Coronal	50	Coch.		Male	1897	May be an early fetus
100	E,27	Poor	?	P	Sagittal	50	Al. coch.	57.5	?	1897
108	E, 28 (est.)	Poor	Piurosulph. acid	P	Sagittal	45	Borax carm.	52.5	Male	1897
227	E30 Ch, 60x40x20	Poor	Formalin	P	Sagittal	50, 100	Al. coch.	54	Female	1903
417	E,32 Ch., 0x60x40	Good	Formalin	P	Transverse	100	Al. coch.	58.5	Female	1907
756A	E, 27 Ch. 60x45x35	Good	Formalin	P	Coronal	50	Al. coch.	56	Male	1913
882	E , 28 Ch, 80x80x40	Good	Formalin	P	Transverse	40	Multiple	53	8	1913
950	E, 29	Good	Formol	P	Transverse	50	Al. coch.	54	Male	1914
1199	E.,26 Ch,, 60x40x30	Good	Formalin	C	Coronal	40	(Stain - Haematoxylin Eosin) aur , or. G.	54.5	Male	1915
1535	E , 28 Ch. 50x45 x15	Poor	Formalin	P	Transverse	40	(Stain - Haematoxylin Eosin)	495	Female	1916
1945	E., 27.3 Ch., 83x53x22.5	Good	Formalin	C-P	Transverse	50	(Stain - Haematoxylin Eosin) aur., or. G.	48	Male	1917
2561	E., 27 .5	Good	Formalin	C-P	Transverse	25	(Stain - Haematoxylin Eosin) aur., or. G.	48 .5	Male	1919
4205	E., 29.5	Good	Bouin	P	Transverse	50	A1. coch.	55.5	Female	1923
4289	E., 32.2 Ch., 52x35x25	Good	Formalin	P	Transverse	15, 20	A1. coch., Mallory	59	Female	1923
4525	E., 30	Good	Formalin	P	Sagittal	20	(Stain - Haematoxylin Eosin)	57	Male	1924
4570	E, 30.7 Ch., 52X50X28	Exc.	Bouin	P	Transverse.	15	(Stain - Haematoxylin Eosin) , phlox.	55	Male	1924
5154	E.,32	Good	Bouin	P	Transverse	20	(Stain - Haematoxylin Eosin)	59.5	Male	1926
5422	E., 27	Good	Formalin	P	Sagittal	40	(Stain - Haematoxylin Eosin)	52.5	Female	1927
5621A	E., 27.5	Good	Formalin	P	Transverse	20	(Stain - Haematoxylin Eosin)	52.5	Male	1927	Other twin has spina bifida and fused kidneys
5725	E, 23	Good	Formalin	P	Coronal	25	(Stain - Haematoxylin Eosin) aur., or. G.	50.5	Female	1928
6573	E,31.5	Good	Bouin	C	Transverse	20	(Stain - Haematoxylin Eosin)	58.5	Female	1932
7425	E, 27	Exc.	Bouin	C-P	Coronal	20	(Stain - Haematoxylin Eosin)	47	Female	1937	Ag added
9226	E, 31	Exc.	Formalin	C—P	Transverse	12	Azan	?	Female	1954
D.122	E, 27	Exc.	?	?	Transverse	19	Ag	?	?	1976	Yntema and Truex
Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined.

Fetal

Fetal Development

Carnegie Collection - Fetal
Serial No.	Size CRL (mm)	Grade	Fixative	Embedding Medium	Plane	Thinness (µm)	Stain	Point Score	Sex	Year	Notes
95	40										catalogued as CRL 40 but development suggests 50 stage. Spinal cord - Kunitomo (1920) Colon - Lineback (1920)
96	50										Brain venous sinuses - Streeter (1915) Spinal cord - Kunitomo (1920) Brain vascular - Streeter (1921) Brain weight - Jenkins (1921)
142	125										Spinal cord - Kunitomo (1920)
145	33										Spinal cord - Kunitomo (1920)
184	50										34 vertebrae, 31 spinal ganglia, Spinal cord - Kunitomo (1920)
211	33										34 vertebra, 31 spinal ganglia, Spinal cord - Kunitomo (1920)
217	45								Male		Genital - Spaulding (1921)
300	73										85 days, Bone ossification - Mall (1906)
362	30										Spinal cord - Kunitomo (1920)
448	52										Colon - Lineback (1920)
449	36										Spinal cord - Kunitomo (1920)
538
590	21 to 23								Male		Genital - Spaulding (1921)
607	37								Male		Genital - Spaulding (1921)
625	220										Temporomandibular joint - Moffatt (1957)
662	80										Spinal cord - Kunitomo (1920)
693	45								Male		Genital - Spaulding (1921)
847	58.8								Male		Genital - Spaulding (1921)
858	57.25										Temporomandibular joint - Moffatt (1957)
922	37
928	120										Spinal cord - Kunitomo (1920)
948	45								Male		Genital - Spaulding (1921)
972	37										34 vertebrae, 30 spinal ganglia, Spinal cord - Kunitomo (1920)
1318	37										Temporomandibular joint - Moffatt (1957)
1388	51								Female		Genital - Spaulding (1921)
1455	78.5										Temporomandibular joint - Moffatt (1957)
1591	36										subcutaneous vascular plexus - Finley (1923)
1656	67										34 vertebrae, Spinal cord - Kunitomo (1920)
1686	40								Male		Genital - Spaulding (1921)
3990	49										Temporomandibular joint - Moffatt (1957)
4473	43					20					Spinal cord meninges - Sensenig (1951)
4475	48					20					Spinal cord meninges - Sensenig (1951)
5652	49										Temporomandibular joint - Moffatt (1957)
6581	75										Temporomandibular joint - Moffatt (1957)
7218	80					20 um					Spinal cord meninges - Sensenig (1951)
1597b	47								Female		Genital - Spaulding (1921)
2250a	40								Female		Genital - Spaulding (1921)
2250b	36								Female		Genital - Spaulding (1921)
This table currently contains only has embryo number information. Abbreviations Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections. Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P). Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution) Stain - ? - unknown or not determined.
References
Fertilization and Gestational Age - Crown-Rump Length (ultrasound)  Fertilization Age (days) Gestational Age GA (week.day) Crown-Rump Length (mm) 37 5.2 1 38 5.3 2 39 5.4 3 40 55 3 41 5.6 4 42    Week 4 6 4 43 6.1 5 44 6.2 6 45 6.3 7 46 6.4 8 47 6.5 9 48 6.6 10 49    Week 5 7 11 50 7.1 11 51 7.2 12 52 7.3 12 53 7.4 13 54 7.5 14 55 7.6 15 56    Week 6 8 17 57 8.1 18 58 8.2 19 59 8.3 20 60 8.4 21 61 8.5 22 62 8.6 22 63    Week 7 9 23 64 9.1 24 65 9.2 26 66 9.3 27 67 9.4 28 68 9.5 29 69 9.6 31 70    Week 8 10 34 71 10.1 36 72 10.2 37 73 10.3 38 74 10.4 39 75 10.5 39 76 10.6 40 77    Week 9 11 44 78 11.1 45 79 11.2 47 80 11.3 48 81 11.4 52 82 11.5 55 83 11.6 56 84    Week 10 12 57 85 12.1 58 86 12.2 60 87 12.3 61 88 12.4 63 89 12.5 64 90 12.6 65 91    Week 11 13 68 92 13.1 70 93 13.2 72 94 13.3 74 95 113.4 76 96 135 77 97 13.6 80 98    Week 12 14 81 99 14.1 84 100 14.2 85 101 14.3 86 102 14.4 87 Reference: Table data measured by ultrasound, adapted from Westerway (2015) PDF and Links: ultrasound | Fetal Development

Human Developmental Anatomy Center Collections

Carnegie collection (1950)

National Museum of Health and Medicine (opened 2011)

The following information are excerpts from the Guide to Collections (2014) p198 - 201. This section of the guide was previously revised by Elizabeth Lockett and Emily Wilson (2009 and 2012).

Arey-Dapeña Pediatric Pathology Collection

24 boxes, 5 binders. Finding aid, restricted, digitized. Over 6,000 lantern slides of various pediatric pathologies, represented by both gross and histological images, created by Marie (Molly) Valdes-Dapeña at the University of Miami. All slides have been digitized and a searchable database is available.

Photo 914E Renal fusion (Arey-Dapeña pathology specimen)

AFIP Sudden Infant Death Collection

• A collection of wet tissue blocks sealed in plastic, glass slides, and detailed case histories documenting cases of Sudden Infant Death Syndrome.
• Materials were originally acquired in the 1970s and 1980s at the National Institute of Child Health and Human Development.

Birth Defects Encyclopedia

• Records and photographs used to compile the Birth Defects Encyclopedia by Mary Louise Buyse.
  • Mary Louise Buyse, M.D., who served as the president and editor-in-chief of the Center for Birth Defects Information Services
• A printed copy of the 1892-page encyclopedia and an electronic file used to produce the print version are included in the collection.

<pubmed>7448380</pubmed>

Carnegie Collection Of Embryology

Carnegie Embryos

• This collection consists principally of serial sections of normal human embryo development in the first eight weeks, as started by the Carnegie Institution of Washington’s Department of Embryology in 1914.
• The collection now forms the core of the Human Developmental Anatomy Center.
• Extensive collateral materials include case histories, photographs, micrographs, models in various media, and comparative materials (mostly rhesus).
• Reprints from the Carnegie Contributions to Embryology, other reprints relating to embryology, films, and personal documents are also available.
• This collection incorporates other embryo collections, such as the Davis Orthopedic Development and Elizabeth Mapelsden Ramsey Collections.
• Partially digitized.

Carnegie Collection Embryos | Embryo (catalogue numbers)

Davis Orthopedic Development Collection

• Part of the Carnegie Collection of Embryology added while curated at the University of California, Davis.
• A large collection of glass slides charting bone growth and development in the human.
• Slides are grouped by structure starting with the head and moving down through the torso and out the extremities.

Hertig Videos

Arthur Tremain Hertig (1904-1990)

• Six VHS tapes of lectures given by [[Dr. Arthur T. Hertig, an embryologist who worked extensively with the Carnegie materials.
• The tapes include two each of: trophoblastic disease, malignant disease of the uterus, and ovarian tumors.
• See also OHA 189, Hertig Collection.

Cornell School Of Veterinary Med. Embryological Collection

* About 150 boxes, 12 binders.

• From the Cornell School of Veterinary Medicine in Ithaca, NY this collection includes human, rat, mouse and guinea pig embryos.
• There is still a large collection of embryonic material at Cornell.

Gaensler Pulmonary Pathology Collection

• A collection of radiographic images and case histories of lung diseases, primarily asbestosis.
• Transferred to the Archives in November 2008 as OHA 163.55.

Hooker-Humphrey Collection

• Originally compiled at the University of Pittsburgh by Davenport Hooker (1887-1965) and Tryphena Humphrey (1902-1971), this collection came to the Museum via the University of Alabama.
• This collection of human and comparative material is stained to highlight nervous system development.
• Sizes of specimens range from 3mm to 310mm. For those wishing to study the development of the human nervous system from birth through maturity to old age.
• This collection is a bridge between the Carnegie Collection focusing on the first eight weeks of development and the neonatal to adult material in the Yakovlev-Haleem Collection.
• http://brainmuseum.org

Charles Sedgwick Minot Embryological Collection

• Embryos from the Harvard School of Medicine, as well as drawings and photographs of the embryos.
• A large collection of reprints, printed lectures, class syllabi, and theses on embryology and related topics.
• The reprint collection was started by Charles S. Minot (1852-1914) in the 1800s and added to through the 1960s.
• The reprint collection also includes personal papers and research notes from Charles Wislocki.
• Finding aid, arranged?

Harvard Collection

Elizabeth Mapelsden Ramsey Collection

• Reprints, personal papers, correspondence, research notes, and artifacts from Elizabeth Mapelsden Ramsey.
• Ramsey was a former curator of the Carnegie Collection, as well as a researcher, lecturer, and teacher.
• The collection is subsumed by the Carnegie Collection of Embryology.

Sensenig Collection

• A small set of comparative material on glass slides from the University of Alabama, compiled by E. Carl Sensenig.
• Arrived along with the Hooker Humphrey comparative materials.

Patten-Burdi Collection

Bradley Patten ( -1971)

• This is a collection of sectioned embryos and fetuses, focused on the second and third trimesters.
• It includes histology sections, reprints, paste-ups for various editions of Patten's Embryology, 2 whole mounts in celloidin, records, and photographic materials.
• Alphonse Burdi was the last curator of the collection at University of Michigan and organized its donation to the museum.
• The Embryology Research Collection at Michigan was established by embryologists George Streeter and G. Carl Huber in the early 1900s with a mission to collect and describe the morphogenesis of human embryos at critical stages in prenatal life.
• For about twenty years up to 1957, the Collection grew in numbers under the leadership of Professor Bradley M. Patten whose primary interests were in heart and cardiovascular development.
  • Human Embryology, by Bradley M. Patten, Ph.D. New York: McGraw-Hill Book Company, 1st edn. 1946; 2nd edn. 1953; 3rd edn. 1968
• After 1957 the collection, under Professor Alphonse Burdi, grew with a focus on the embryology of craniofacial regions and related birth defect syndromes.
• In 2004 the Burdi-Patten Michigan Embryology Collection was deeded by the University of Michigan to the Human Developmental Anatomy Center (HDAC) at the Armed Forces Institute of Pathology in Washington, DC.

Links: George Streeter | G. Carl Huber | Bradley Patten | Patten Embryology Research Collection (1999)

Berkowitz Cleft Palate

• The collection of orthodontist Samuel Berkowitz includes panoramic x-rays, cephalometrics, patient records, dental casts, digital records, and educational materials of his longitudinal studies on treatment of cleft lip and palate.
• Dr. Berkowitz was a Clinical Professor of Pediatrics and Surgery associated with the South Florida Craniofacial Anomalies Program at the University of Miami School of Medicine and is now active in developing teaching materials on cleft palate.

Selected Reference Berkowitz S. (2014). The present state of treatment in cleft palate: what still needs to be done. Cleft Palate Craniofac. J. , 51, 369-70. PMID: 24524205 DOI.

Search Pubmed: Berkowitz S (Author)

Koering Collection

• A former student of George Corner, Dr. Marilyn J. Koering (1938-2008) studied follicle and ovary development.
• The collection consists of histology slides (mostly rhesus), photographs, micrographs, personal reprints, reprints supporting research, and research notes.

Stanford Mouse

• A teaching set collection of mouse embryo development.

Ob-Gyn Lantern Slides

• A collection of lantern slides used in teaching obstetrics and gynecology dating from 1915-1940.
• Much of the contained data pertaining to New York clinics (e.g., Berwind and Bellevue).
• The set represents popular contemporary topics in the field.

George Washington University Medical School Fetal Development Collection

• A collection of whole embryos and fetuses, with associated records, used for teaching and display by GWU Medical College, created and donated by Frank D. Allan.

Richardson Pediatric Slide Collection

• Notebooks of 35mm slides of lecture text and clinical pictures of various pediatric pathologies and diseases from the pediatric practice of Martyn E. Richardson.
• Topics include musculoskeletal syndromes; neurosensory; blood and RE; cardiovascular; endocrine; genitor-urinary; infectious diseases; drug company slides; newborn; gastrointestinal; nutrition; skin; respiratory; allergy; collagen diseases; growth and development; and child abuse.
• 10 binders and 71 loose pages. Arranged, unrestricted.

Lockard Reprint Collection

• Reprint collection of embryologist Isabel Lockard. May also contain photographs.
• 6 boxes. No finding aid, unarranged, inactive, unrestricted.
• Isabel Lockard, Ph.D., (1915 -2011) - neuroanatomist at Georgetown University (1947-1952). At Medical College of South Carolina’s Department of Anatomy as Assistant Professor (1952). Second full-time female faculty member in the College of Medicine, and the first neuroanatomist at the Medical University to retain tenure in the position over the long term. She was a Professor 1969-1985). Retirement and appointed as Professor Emeritus in 1985.

Links: Waring Historical Library

Donald L. Kimmel

Kimmel Collection

• Histology slides of 25 human embryo specimens from research by Donald L. Kimmel and Elizabeth Moyer, Temple University, 1940s-50s.

Search PubMed: KIMMEL DL (Author)

Osborne O. Heard Collection

Osborne O. Heard

• This collection is primarily a record of anatomical modeler Osborne O. Heard's (1890-1983) work while at the Carnegie Institute of Washington's Department of Embryology.
• It includes correspondence, early photographs of the department, and Mr. Heard.
• Artwork includes sketches by James Didusch and mechanical drawings of various devices designed by Mr. Heard for the CIW and other departments at John Hopkins. Dates 1919-1989.

Johns Hopkins Medical School Lantern Slides

• A collection of 2 boxes consisting of 500+ lantern slides used at Johns Hopkins University Medical School for teaching embryology, 1920-2009.
• Includes a number of images from the Carnegie Collection of Embryology.
• Arrived along with models that were originally from, and are now re-incorporated into, the Carnegie Collection.

References

Skidmore FD. An Analysis of the Age and Size of 483 Human Embryos. (1976) Teratology 15: 97-102. PMID 557243

O'Rahilly R. and Müller F. Developmental Stages in Human Embryos. Contrib. Embryol., Carnegie Inst. Wash. 637 (1987).

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Cite this page: Hill, M.A. (2023, December 2) Embryology Carnegie Collection. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Carnegie_Collection

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© Dr Mark Hill 2023, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G