BGD Lecture - Face and Ear Development
|Embryology - 22 Apr 2018 Expand to Translate|
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| The face is the anatomical feature which is truly unique to each human, though the basis of its general development is identical for all humans and similar to that seem for other species. The face has a complex origin arising from a number of head structures and sensitive to a number of teratogens during critical periods of its development. The related structures of upper lip and palate significantly contribute to the majority of face abnormalities.
The head and neck structures are more than just the face, and are derived from pharyngeal arches 1 - 6 with the face forming from arch 1 and 2 and the frontonasal prominence. Each arch contains similar Arch components derived from endoderm, mesoderm, neural crest and ectoderm.
Because the head contains many different structures also review notes on Special Senses, Respiratory, Integumentary (Teeth), Endocrine (thyroid, parathyroid, pituitary, thymus) and Ultrasound- Cleft lip/palate.
We use the sense of balance and hearing to position ourselves in space, sense our surrounding environment, and to communicate. Importantly hearing is linked into postnatal neurological development (milestones) involved with language and learning.
Portions of the ear appear very early in development as specialized region (otic placode) on the embryo surface that sinks into the mesenchyme to form a vesicle (otic vesicle = otocyst) that form the inner ear.
| To introduce the developmental embryology of both the face and ear, and their associated abnormalities.
|Buccopharyngeal Membrane and Pharynx|
These images of the Week 4 embryo (23 - 26 days, Stage 11) show the breakdown of the buccopharyngeal (oral) membrane.
The cavity within the pharyngeal arches forms the pharynx.
- begins at the buccopharyngeal membrane (oral membrane), apposition of ectoderm with endoderm (no mesoderm between)
- expands behind pharyngeal arches
- narrows at glottis and bifurcation of gastrointestinal (oesophagus) and respiratory (trachea) systems
- regions on roof, walls and floor have important contributions to endocrine in oral and neck regions
- also contributes to tongue development
|Week 4 - Arches (stage 11)|
Pharyngeal Arch Components
Major features to identify for each: arch, pouch, groove and membrane. Contribute to the formation of head and neck and in the human appear at the 4th week. The first arch contributes the majority of upper and lower jaw structures.
Pharyngeal Arch Development
- branchial arch (Greek. branchia = gill)
- arch consists of all 3 trilaminar embryo layers
- ectoderm- outside surface and core neural crest
- mesoderm- core of mesenchyme
- endoderm- inside pharynx
|Pharynx Week 4 (stage 13)|
- Mesenchyme invaded by neural crest generating connective tissue components
- cartilage, bone, ligaments
- arises from midbrain and hindbrain region
|Neural Crest Migration|
|Chicken Embryo - DiI-labelled neural crest cells towards the branchial arches.
White rings indicate migration of individual cells. Each image represents 10 confocal sections separated by 10 microns.
Mouse Embryo - GFP-labelled cranial neural crest cells in embryonic mouse (E9.5).
Each arch contains: artery, cartilage, nerve, muscular component
Arches and Phanynx Form the face, tongue, lips, jaws, palate, pharynx and neck cranial nerves, sense organ components, glands
- Humans have 5 arches - 1, 2, 3, 4, 6 (Arch 5 does not form or regresses rapidly)
- form in rostro-caudal sequence, Arch 1 to 6 (from week 4 onwards)
- arch 1 and 2 appear at time of closure of cranial neuropore
- Face - mainly arch 1 and 2
- Neck components - arch 3 and 4 (arch 4 and 6 fuse)
- groove - (cleft) externally separates each arch (only first pair persist as external auditory meatus)
- pouch - internally separates each arch (pockets out from the pharynx)
- membrane - ectoderm and endoderm contact regions (only first pair persist as tympanic membrane )
Pharyngeal Arch 1 (Mandibular Arch) has 2 prominences
- smaller upper- maxillary forms maxilla, zygomatic bone and squamous part of temporal
- larger lower- mandibular, forms mandible
Pharyngeal Arch 2 (Hyoid Arch)
- forms most of hyoid bone
Arch 3 and 4
- neck structures
Embryo Blood Flow - placental vein -> liver -> heart -> truncus arteriosus -> aortic sac -> arch arteries -> dorsal aorta -> placental artery
Merkel's cartilage (first pharyngeal arch)
|Pharyngeal Arch - Summary Table|
The arch pouches contribute to endocrine organ development, except for the thyroid and pituitary. Note endocrine development will be covered in detail in a later BGD lecture.
|Thyroid Gland||Anterior Pituitary|
Begins week 4 centered around stomodeum, external depression at oral membrane
5 initial primordia from neural crest mesenchyme (week 4)
- single frontonasal prominence (FNP) - forms forehead, nose dorsum and apex
- nasal placodes develop later bilateral, pushed medially
- paired maxillary prominences - form upper cheek and upper lip
- paired mandibular prominences - lower cheek, chin and lower lip
- Cranium (Neurocranium) surrounds brain.
- dermatocranium (membranous) - skull calvarial vault develops from intramembranous ossification
- chondrocranium (cartilaginous) - skull base develops from endochondral ossification
- 8 bones - occipital, 2 parietals, frontal, 2 temporals, sphenoidal, ethmoidal.
- Face (Viscerocranium) development of the facial bones
- 14 bones - 2 nasals, 2 maxillæ, 2 lacrimals, 2 zygomatics, 2 palatines, 2 inferior nasal conchæ, vomer, mandible.
Calveria - bone has no cartilage (direct ossification of mesenchyme)
- bones do not fuse, fibrous sutures
- allow distortion to pass through birth canal
- allow growth of the brain
- 6 fontanelles - posterior closes at 3 months, anterior closes at 18 months
- continues postnatally - fontanelle allow head distortion on birth and early growth
- bone plates remain unfused to allow growth, puberty growth of face
- Links: Skull Development
- During week 4 a series of thickened surface ectodermal patches form in pairs rostro-caudally in the head region.
- Recent research suggests that all sensory placodes may arise from common panplacodal primordium origin around the neural plate, and then differentiate to eventually have different developmental fates.
- These sensory placodes will later contribute key components of each of our special senses (vision, hearing and smell). Other species have a number of additional placodes which form other sensory structures (fish, lateral line receptor). Note that their initial postion on the developing head is significantly different to their final position in the future sensory system
- Carnegie stage 12 still visible on embryo surface.
- Carnegie stage 13/14 embryo (shown below) the otic placode has sunk from the surface ectoderm to form a hollow epithelial ball, the otocyst, which now lies beneath the surface surrounded by mesenchyme (mesoderm). The epithelia of this ball varies in thickness and has begun to distort, it will eventually form the inner ear membranous labyrinth.
- (optic placode) lies on the surface, adjacent to the outpocketing of the nervous system (which will for the retina) and will form the lens.
- Has 2 components (medial and lateral) and will form the nose olefactory epithelium.
|Week 5||Week 8|
Otocyst (Week 5, Stage 13)
Inner Ear (Week 8, Stage 22)
- Inner Ear Labyrinth
- Cochlea - Otic vesicle - Otic placode (ectoderm)
- Semicircular canals - Otic vesicle - Otic placode (ectoderm)
- Saccule and utricle - Otic vesicle - Otic placode (ectoderm)
- Cranial Nerve VIII
- Auditory component - Otic vesicle and neural crest (ectoderm)
- Vestibular component - Otic vesicle and neural crest (ectoderm)
Pharyngeal arch cartilages
- Auricle - Pharyngeal Arches 1 and 2 (ectoderm, mesoderm)
- form from 6 hillocks (week 5) 3 on each of arch 1 and 2
- External Auditory Meatus - Pharyngeal Arch 1 groove or cleft (ectoderm)
- Tympanic Membrane - Pharyngeal Arch 1 membrane (ectoderm, mesoderm, endoderm)
| Adult - longer (twice as long), wider and runs at approximately 45 degrees to the horizontal, tube is opened by two separate muscles (tensor palati and levator palati)
- Auditory tube = Eustachian, otopharyngeal or pharyngotympanic tube.
- Connects middle ear cavity to nasopharynx portion of pharynx
- Ventilation - pressure equalization in the middle ear
- Clearance - allow fluid drainage from the middle ear Tube is normally closed and opened by muscles
- Links: Hearing Development
|Primary palate, fusion in the human embryo between week 6-7 (stage 17 and 18, GA Week 8-9), from an epithelial seam to the mesenchymal bridge.|
Secondary palate, fusion in the human embryo in week 9 (GA week 11). This requires the early palatal shelves growth, elevation and fusion during the early embryonic period. The fusion event is to both each other and the primary palate. palatal shelf elevation | secondary palate
- Ectoderm of the first arch surrounding the stomodeum forms the epithelium lining the buccal cavity.
- Also the salivary glands, enamel of the teeth, epithelium of the body of the tongue.
- As the tongue develops "inside" the floor of the oral cavity, it is not readily visible in the external views of the embryonic (Carnegie) stages of development.
- Contributions from all arches, which changes with time
- begins as swelling rostral to foramen cecum, median tongue bud
- Arch 1 - oral part of tongue (ant 3/2)
- Arch 2 - initial contribution to surface is lost
- Arch 3 - pharyngeal part of tongue (post 1/3)
- Arch 4 - epiglottis and adjacent regions
- Tongue muscles originate from the somites. Tongue muscles develop before masticatory muscles and is completed by birth.
- Masticatory muscles originate from the somitomeres. These muscles develop late and are not complete even at birth.
- epithelial buds in oral cavity (wk 6-7) extend into mesenchyme
- parotid, submandibular, sublingual
Cleft Lip and Palate
|cleft palate||unilateral cleft lip and palate||bilateral cleft lip and palate|
- 300+ different abnormalities, different cleft forms and extent, upper lip and ant. maxilla, hard and soft palate
- Cleft palate has the International Classification of Diseases code 749.0.
- In Australia the national rate (1982-1992) for this abnormalitity in births was 4.8 - 6/10,000 births, which represented 1,530 infants 5.5% were stillborn and 11.5% liveborn died during neonatal period and slightly more common in twin births than singleton.
- The International Classification of Diseases code 749.1 for isolated cleft lip and 749.2 for cleft lip with cleft palate.
- In Australia the national rate (1982-1992) for this abnormalitity was 8.1 - 9.9 /10,000 births. Of 2,465 infants 6.2% were stillborn and 7.8% liveborn died during neonatal period and the rate was similar in singleton and twin births.
|Palate Links: palate | cleft lip and palate | cleft palate | Head Development | Category:Palate|
First Arch Syndrome
- There are 2 major types of associated first arch syndromes, Treacher Collins (Mandibulofacial dysostosis) and Pierre Robin (Pierre Robin complex or sequence), both result in extensive facial abnormalites.
Treacher Collins Syndrome
Pierre Robin Syndrome
- Hypoplasia of the mandible, cleft palate, eye and ear defects.
- Initial defect is small mandible (micrognathia) resulting in posterior displacement of tongue and a bilateral cleft palate.
- absence of thymus and parathyroid glands, 3rd and 4th pouch do not form
- disturbance of cervical neural crest migration
- Many different types
- extremely rare
- shh abnormality
- Retinoic Acid - present in skin ointments
- 1988 associated with facial developmental abnormalities
Fetal Alcohol Syndrome
Due to alcohol in early development (week 3+) leading to both facial and neurological abnormalities
- lowered ears, small face, mild+ retardation
- Microcephaly - leads to small head circumference
- Short Palpebral fissure - opening of eye
- Epicanthal folds - fold of skin at inside of corner of eye
- Flat midface
- Low nasal bridge
- Indistinct Philtrum - vertical grooves between nose and mouth
- Thin upper lip
- Micrognathia - small jaw
Exposure of embryos in vitro to ethanol simulates premature differentiation of prechondrogenic mesenchyme of the facial primordia (1999)
- Links: Fetal Alcohol Syndrome
Table - Structures derived from Arches
|1 (maxillary/mandibular)||trigeminal (V)||mandible, maxilla, malleus, incus||ant lig of malleus, sphenomandibular ligament|
|2 (hyoid)||facial (VII)||stapes, styloid process, lesser cornu of hyoid, upper part of body of hyoid bone||stylohyoid ligament|
|3||glossopharyngeal (IX)||greater cornu of hyoid, lower part of body of hyoid bone|
|4 & 6||superior laryngeal and recurrent laryngeal branch of vagus (X)||thyroid, cricoid, arytenoid, corniculate and cuneform cartilages|
Structures derived from Pouches
Each pouch is lined with endoderm and generates specific structures.
||Overall Structure||Specific Structures|
||tubotympanic recess||tympanic membrane, tympanic cavity, mastoid antrum, auditory tube|
||intratonsillar cleft||crypts of palatine tonsil, lymphatic nodules of palatine tonsil|
||inferior parathyroid gland, thymus gland|
||superior parathyroid gland, ultimobranchial body|
||becomes part of 4th pouch|
Structures derived from Grooves
Only the first groove differentiates into an adult structure and forms part of the external acoustic meatus.
Structures derived from Membranes
At the bottom of each groove lies the membrane which is formed from the contact region of ectodermal groove and endodermal pouch.
Only the first membrane differentiates into an adult structure and forms the tympanic membrane.
- Developmental Biology by Gilbert, Scott F. Sunderland (MA): Sinauer Associates, Inc.; c2000 Figure 1.3. Pharyngeal arches | Table 13.2. Some derivatives of the pharyngeal arches | The Cranial Neural Crest
- Madame Curie Bioscience Database Chapters taken from the Madame Curie Bioscience Database (formerly, Eurekah Bioscience Database) Cranial Neural Crest and Development of the Head Skeleton | Neural Crest Cells and the Community of Plan for Craniofacial Development: Historical Debates and Current Perspectives
- Bookshelf pharyngeal arch | head development | face development
- Pubmed pharyngeal arch | head development | face development |
- palate - The roof of the mouth (oral cavity) a structure which separates the oral from the nasal cavity. Develops as two lateral palatal shelves which grow and fuse in the midline. Initally a primary palate forms with fusion of the maxillary processes with the nasal processes in early face formation. Later the secondary palate forms the anterior hard palate which will ossify and separate the oral and nasal cavities. The posterior part of the palate is called the soft palate (velum, muscular palate) and contains no bone. Abnormalities of palatal shelf fusion can lead to cleft palate. (More? Palate Development | Head | Head Abnormalities)
- palatogenesis - The process of palate formation, divided into primary and secondary palate development. (More? Palate Development | Head | Head Abnormalities)
- pharyngeal arch - (branchial arch, Greek, branchial = gill) These are a series of externally visible anterior tissue bands lying under the early brain that give rise to the structures of the head and neck. In humans, five arches form (1,2,3,4 and 6) but only four are externally visible on the embryo. Each arch has initially identical structures: an internal endodermal pouch, a mesenchymal (mesoderm and neural crest) core, a membrane (endoderm and ectoderm) and external cleft (ectoderm). Each arch mesenchymal core also contains similar components: blood vessel, nerve, muscular, cartilage. Each arch though initially formed from similar components will differentiate to form different head and neck structures. (More? | Head Development | Endocrine | Neural Crest)
- pharyngeal arch artery - Each early developing pharyngeal arch contains a lateral pair of arteries arising from the aortic sac, above the heart, and running into the dorsal aorta. later in development these arch arteries are extensively remodelled to form specific components of the vascular system. Pharyngeal Arch 1 arteries are mainly lost and forms part of maxillary artery. Pharyngeal Arch 2 arteries remains to form the stapedial arteries. Pharyngeal Arch 3 arteries forms the common carotid arteries, internal carotid arteries in the neck. Pharyngeal Arch 4 arteries will form part of aortic arch (left arch artery) and part right subclavian artery (right arch artery) Pharyngeal Arch 6 arteries form part of left pulmonary artery (left arch artery) and part of right pulmonary artery (right arch artery). (More? | Head Development | Cardiovascular)
- pharyngeal arch cartilage - Each early developing pharyngeal arch contains a horseshoe shaped band of cartilage that acts as a template and contributes to the development of head and neck bony and cartilagenous features, including the middle ear bones. Pharyngeal Arch 1 cartilage (Meckel‚Äôs cartilage) dorsal ends form malleus and incus midpart forms ligaments (ant. malleus, sphenomandibular) ventral part forms mandible template. Pharyngeal Arch 2 cartilage (Reichert‚Äôs cartilage) dorsal ends form stapes and Temporal bone styloid process, ventral part ossifies to form hyoid bone components, lesser cornu and superior body. Pharyngeal Arch 3 cartilage forms hyoid components, greater cornu and inferior part of hyoid. Pharyngeal Arch 4 and 6 cartilage forms laryngeal cartilages except epiglottis (from hypobranchial eminence). (More? Head Development | Middle Ear)
- pharyngeal arch nerve - each early developing pharyngeal arch contains the developing cranial nerves, as a pair, within the arch mesenchyme. Each cranial nerve is numbered (roman numeral) in rostrocaudal sequence and also has a specific name. The cranial nerve within each arch often relates to the other structures formed from taht arch. Pharyngeal Arch 1 contains the trigeminal nerve (CN V, cranial nerve 5). Pharyngeal Arch 2 contains the facial nerve (CN VII, cranial nerve 7). Pharyngeal Arch 3 contains the glossopharyngeal nerve (CN IX, cranial nerve 9) Pharyngeal Arch 4 and 6 contains the Vagus (CN X cranial nerve 10), forming the adult superior laryngeal and recurrent laryngeal branches. (More? | Head Development | Neural | Neural Crest)
- pharyngeal arch pouch - An out-pocketing of the endoderm lined pharynx occurring between each developing pharyngeal arch. Each of the pharyngeal arch pouches contributes different components of the head and neck, either cavities or endocrine tissues. Pharyngeal Arch 1 pouch elongates to form tubotympanic recess tympanic cavity, mastoid antrum and auditory tube (Eustachian tube). Pharyngeal Arch 2 pouch forms the tonsillar sinus and is later mostly oblierated by palatine tonsil. Pharyngeal Arch 3 pouch forms the inferior parathyroid and thymus. Pharyngeal Arch 4 pouch forms the superior parathyroid, parafollicular cells of Thyroid. (More? Middle Ear | Thyroid | Parathyroid | Thymus | Endocrine | Head Development
- pharyngotympanic tube - (auditory tube, eustachian tube, otopharyngeal tube) A narrow canal connecting the middle ear space to the back of the oral cavity. The tube allows ventilation, protection and clearance for the middle ear cavity. Ventilation is the pressure equalization in the middle ear. Clearance is to allow fluid drainage from the middle ear. Embryonic origin is from the first pharyngeal pouch. In development, the canal is initially both horizontal, short and very narrow leading to poor drainage and easy blockage. (More? Middle Ear | Hearing | Hearing Abnormalities)
- pharynx - (throat) Forms the initial segment of the upper respiratory tract divided anatomically into three regions: nasopharynx, oropharynx, and laryngopharynx (hypopharynx). Anatomically extends from the base of the skull to the level of the sixth cervical vertebra. (More? Respiratory System Development)
| Hearing and Balance Development
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Cite this page: Hill, M.A. (2018, April 22) Embryology BGD Lecture - Face and Ear Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/BGD_Lecture_-_Face_and_Ear_Development
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