Introduction
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Facts
week 4 to week 5, 28 - 32 days.
The embryos have a crown rump length (CRL) of 4 - 6 mm and somite number 30 pairs.
Gestational Age GA - end week 6 beginning week 7
Summary
- Ectoderm: Neural tube continues to close, Caudal neuropore closes, forebrain
- Mesoderm: continued segmentation of paraxial mesoderm (21 - 29 somite pairs), heart prominence
- Head: 1st, 2nd and 3rd pharyngeal arch, forebrain, site of lens placode, site of otic placode, stomodeum
- Body: heart, liver, umbilical, early upper limb bulge
See also Events
Features
- 30 somites, forebrain, site of lens placode, site of otic placode , stomodeum, 1st pharyngeal arch, 2nd pharyngeal arch, 3rd pharyngeal arch, heart prominence, somites
- Identify: forebrain, site of lens placode, site of otic placode, stomodeum, 1st pharyngeal arch, 2nd pharyngeal arch, 3rd pharyngeal arch, heart prominence, somite
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- Links: Week 4 | Week 5 | Somitogenesis | Placodes | Head | Lecture - Early Vascular | Lecture - Gastrointestinal | Lecture - Head Development | Science Practical - Gastrointestinal | Science Practical - Head | Category:Carnegie Stage 13 | Stage 14
Carnegie Stage 13 Movies: Gastrointestinal Tract | Cardiovascular | Central Nervous System | Carnegie Stage 13 | Stage 22 Movies | Movies
- The original animations were part of the UNSW Independent Learning Project (ILP) prepared by Aashish Kumar (2006).
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- Carnegie Stages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | About Stages | Timeline
Bright Field
- Embryo Links: Embryo and Placenta | Embryo | Embryo (label) | Embryo (animated) | Embryo (animated large) | Head region | Head region (label) | Body region | Body region (label) | Carnegie stage 13
Embryo Virtual Slide
Scanning EM
Kyoto Collection
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Upper half of embryo shown in median sagittal plane. Scale bar 1 mm.
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Upper half of embryo shown in ventral view. Scale bar 0.5 mm.
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Oral cavity floor. Scale bar 0.5 mm.
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Image source: The Kyoto Collection images are reproduced with the permission of Prof. Kohei Shiota and Prof. Shigehito Yamada, Anatomy and Developmental Biology, Kyoto University Graduate School of Medicine, Kyoto, Japan for educational purposes only and cannot be reproduced electronically or in writing without permission.
Carnegie Collection
- Carnegie stage 13: 6473 left | 6473 dorsal | 6473 right | 6469 right | 8066 dorsal | 8066 left | 8119 left | 7433 right | 7433 ventral
Carnegie Collection Embryos - Stage 13
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Serial No. |
Size (mm) |
Grade |
Fixative |
Embedding Medium |
Plane |
Thinness (µm) |
Stain |
Year |
Notes
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1 |
E.,4.5 Ch., 30x30 |
Poor |
Salicylic acid |
P |
Transverse |
10 |
Hemat. |
1887 |
Obtained by Mall while student
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19 |
E., 5.5 Ch., 18x14 |
Poor |
p |
? |
Transverse |
20 |
Al. coch. |
1895 |
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98 |
E., 4 Ch., 24x16x9 |
Poor |
p |
P |
Transverse |
20 |
Al. coch. |
1896 |
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76 |
E., 4.5 Ch., 22x20 |
Poor |
Alc. |
P |
Transverse |
20 |
Al. coch. |
1897 |
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112 |
E., 4 |
Poor |
p |
P |
Sagittal |
10 |
Al. coch. |
p |
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116 |
E., 5 |
Poor |
p |
? |
Sagittal |
10 |
Al. coch. |
1898 |
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148 |
E.,4.3 Ch., 17x14x10 |
Poor |
Alc. |
P |
Coronal |
10 |
(Stain - Haematoxylin Eosin) |
1899 |
Abnormal. Nasal discs fused
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186 |
E.,3.5 Ch., 25x20x15 |
Poor |
Alc. |
P |
Transverse |
20 |
Al. coch. |
1901 |
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239 |
E., 3.0 |
Poor |
Formalin |
P |
Transverse |
10 |
(Stain - Haematoxylin Eosin) |
1903 |
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248 |
E., 4.5 Ch., 30x23x15 |
Poor |
p |
? |
Coronal |
50 |
Al. coch. |
1904 |
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407 |
E.,4 Ch., 14x13X7 |
Poor |
Formalin |
? |
Transverse |
40 |
Al. coch. |
1907 |
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463 |
E., 3.9 Ch., 17x12x7 |
Good |
Formalin |
P |
Coronal |
10 |
Al. coch. |
1910 |
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523 |
E., 5 Ch., 25x25x15 |
Fair |
Formalin |
P |
Transverse |
p |
Al. coch. |
1911 |
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588 |
E., 4.0 Ch., 19x15x8 |
Good |
Corros. acetic |
P |
Coronal |
15 |
(Stain - Haematoxylin Eosin) |
1912 |
Advanced
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786 |
E., 4.5 Ch., 19x10x10 |
Poor |
Alc. |
P |
Sagittal |
15 |
Al. coch. |
1913 |
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800 |
E., 6.0 |
Good |
Corros. acetic |
P |
Transverse |
10 |
H |
1913 |
Curettage. Anencephaly
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808 |
E.,4.0 |
Poor |
Corros. acetic |
P |
Transverse |
15 |
Al. coch. |
1914 |
Tubal Incomplete
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826 |
E., 5.0 Ch., 13x13x9 |
Good |
Formalin |
P |
Transverse |
20 |
Al. coch. |
1914 |
Shrunken
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836 |
E.,4.0 Ch., 22x18x11 |
Exc. |
Corros. acetic |
P |
Transverse |
15 |
Al. coch. |
1914 |
Less advanced
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963 |
E.,4.0 Ch., 23x18x16 |
Good |
Formalin |
P |
Coronal |
20 |
Al. coch. |
1914 |
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1075 |
E.,6.0 Ch., 46x32x20 |
Exc. |
Corros. acetic |
P |
Coronal |
20 |
(Stain - Haematoxylin Eosin) or. G. |
1915 |
Most advanced in group
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3956 |
E., 4.0 |
Poor |
Formalin |
P |
Transverse |
20 |
Al. coch. |
1922 |
Tubal Incomplete
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4046 |
E.,5 Ch., 22x20x20 |
Poor |
Formalin |
P |
Transverse |
50 |
Al. coch. |
1922 |
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5541 |
E., 6.0 Ch., 35x30x20 |
Good |
Formalin |
P |
Transverse |
10 |
Al. coch., eosin |
1927 |
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5682 |
E., 5.3 Ch., 29x25x13 |
Poor |
Formalin |
P |
Coronal |
20 |
Al. coch. |
1928 |
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5874 |
E., 4.8 |
Exc. |
Bouin |
P |
Transverse |
10 |
(Stain - Haematoxylin Eosin) |
1929 |
Hysterotomy. Bromides only
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6032 |
E., 5.8 Ch., 30x24x13 |
Poor |
Formalin |
P |
P |
? |
p |
1929 |
Not good enough to cut
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6469 |
E., 5.0 Ch., 25x18x18 |
Poor |
Formalin |
P |
P |
P |
P |
1932 |
Fragmented on cutting. Not saved
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6473 |
E., 5.0 Ch, 30x30x15 |
Exc. |
Formalin |
C-P |
Coronal |
6 |
Al. coch. |
1932 |
Less advanced. Ag added
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7433 |
E., 5.2 Ch., 15x13x13 |
Exc. |
Formalin |
C-P |
Coronal |
8 |
(Stain - Haematoxylin Eosin) |
1937 |
Tubal
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7618 |
E, 48 Ch, 18x15x15 |
Exc. |
Bouin |
C-P |
Coronal |
10 |
(Stain - Haematoxylin Eosin) |
1939 |
Hystereaomy. Advanced. Ag added
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7669 |
E, 5.0 Ch., 23x16x14 |
Good |
Formalin |
C-P |
Coronal |
6 |
(Stain - Haematoxylin Eosin) |
1939 |
Hysterectomy. Least advanced in group, Ag added
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7889 |
E, 4.2 |
Exc. |
Bouin |
C-P |
Coronal |
6 |
(Stain - Haematoxylin Eosin) |
1941 |
Hysterectomy
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8066 |
E,53 Ch , 20x18xI8 |
Exc. |
Bouin |
C-P |
Transverse |
8 |
(Stain - Haematoxylin Eosin) |
1942 |
Hysterectomy. Ag added to slide 2
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8119 |
E., 5.3 Ch., 32x28x6.5 |
Exc. |
Bouin |
C-P |
Transverse |
8 |
(Stain - Haematoxylin Eosin) |
1943 |
Hysterectomy
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8147 |
E., 5.2 Ch., 27x21x19 |
Poor |
Formalin |
? |
? |
p |
p |
1943 |
Tubal Not cut
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8239 |
E., 4.3 |
Exc. |
Bouin |
C-P |
Sagittal |
8 |
H. phlox. |
1944 |
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8372 |
E., 5.6 |
Exc. |
Alc.& Bouin |
P |
Transverse |
10 |
Azan |
1946 |
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8581 |
E., 4.8 |
Good |
Kaiserling |
C-P |
Sagittal |
8 |
Azan |
1948 |
Most-advanced third
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8967 |
E., 5.7 |
Exc. |
Acetic Zenker |
C-P |
Transverse |
6 |
(Stain - Haematoxylin Eosin) |
1931 |
Head injured. Univ. Chicago No. H1426
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9296 |
E,4.5 |
Exc. |
C-P |
Coronal |
8 |
Azan |
1955 |
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9297 |
E., 4.5 |
Exc. |
C-P |
Sagittal |
8 |
Azan |
1955 |
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9697 |
E., 5.5 |
Bouin |
1956 |
not cut
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Abbreviations
- Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
- Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
- Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
- Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
- Stain -
- ? - unknown or not determined.
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iBook - Carnegie Embryos
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- iTunes link | iBook Store
- Description - Imagine the excitement of seeing this incredible early period of human development for the first time. Now consider that much of our initial understanding of human development is based upon study of historic embryo collections. You can now look at these historic images of the first 8 weeks after fertilisation and explore for yourself the changes that occur in human development during this key period. This current book is designed as an atlas of the Carnegie embryo stages with some brief notes and additional information covering the first 8 weeks of development. These images are from from the beginning of last century and are one of the earliest documented series of human embryos collected for basic research and medical education on development. I hope you enjoy learning about the amazing early events that begin to make and shape us. This is the second book in a series of educational releases from UNSW Embryology.
- Release: First Edition - Mar 12, 2012 ISBN 978-0-7334-3148-7 Print Length 82 Pages, 25.8 MB Language English.
- PDF Preview version 3.87 MB (Read the associated information, this is an edited educational preview version with many features not functioning).
- The current website also includes numerous embryo images from this textbook (see Embryonic Development and Carnegie Embryos).
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Hill Collection
- Links: Hill Collection
Stage 13 Serial Section Images
These are the original serial images prepared from embryo sections for Embryology practical classes and transferred online in 1996. This embryo shows some features present on the later Stage 14 embryo.
- Links: Carnegie stage 13 - serial sections | Carnegie stage 22 - serial sections | Carnegie stage 22
Labeled Sections
Unlabeled Sections
Stage 13 Movies
Events
- hearing - otic vesicle closes from surface and endolymphatic appendage apparent. A capillary network forms around the otic vesicle with esoderm becomes condensed as the otic capsule.[1] Vestibulocochlear ganglion (vestibular part) and vestibular nerve fires present.
- Vision - By the end of the fourth week the optic vesicle lies close to the surface ectoderm. Optic evagination differentiation allows identification of optic part of retina, future pigmented layer of retina, and optic stalk. The surface ectoderm overlying the optic vesicle, in response to this contact, has thickened to form the lense placode.[2]
References
Additional Images
Stage 13 crown rump length
Stage 13 Optical Projection Tomography
Cross-section showing neural tube, notochord, limb bud
Selected image of above cross-section showing neural tube, notochord, limb bud
Historic
Historic Disclaimer - information about historic embryology pages
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Pages where the terms "Historic" (textbooks, papers, people, recommendations) appear on this site, and sections within pages where this disclaimer appears, indicate that the content and scientific understanding are specific to the time of publication. This means that while some scientific descriptions are still accurate, the terminology and interpretation of the developmental mechanisms reflect the understanding at the time of original publication and those of the preceding periods, these terms, interpretations and recommendations may not reflect our current scientific understanding. (More? Embryology History | Historic Embryology Papers)
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Historic - Human embryo with twenty-seven primitive segments (7 mm., 26 days)
Historic - Human embryo with 28 primitive segments (7.5 mm)
- Carnegie Stages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | About Stages | Timeline
Image Source: Scanning electron micrographs of the Carnegie stages of the early human embryos are reproduced with the permission of Prof Kathy Sulik, from embryos collected by Dr. Vekemans and Tania Attié-Bitach. Images are for educational purposes only and cannot be reproduced electronically or in writing without permission.
Embryonic Development
Cite this page: Hill, M.A. (2024, May 19) Embryology Carnegie stage 13. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Carnegie_stage_13
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