1987 Developmental Stages In Human Embryos - Appendix 1

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O'Rahilly R. and Müller F. Developmental Stages in Human Embryos. Contrib. Embryol., Carnegie Inst. Wash. 637 (1987).

Online Editor Note  
O'Rahilly R. and Müller F. Developmental Stages in Human Embryos. Contrib. Embryol., Carnegie Inst. Wash. 637 (1987).

The original 1987 publication text, figures and tables have been altered in formatting, addition of internal online links, and links to PubMed. Original Document - Copyright © 1987 Carnegie Institution of Washington.

See also the later 2010 paper by the same authors - O'Rahilly R & Müller F. (2010). Developmental stages in human embryos: revised and new measurements. Cells Tissues Organs (Print) , 192, 73-84. PMID: 20185898 DOI.

Links: Embryonic Development | Carnegie Collection | Carnegie Embryos | Ronan O'Rahilly | Fabiola Müller

1987 Stages: Introduction | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | References | Appendix 1 | Appendix 2 | Historic Papers | Embryonic Development
Historic Disclaimer - information about historic embryology pages 
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Pages where the terms "Historic" (textbooks, papers, people, recommendations) appear on this site, and sections within pages where this disclaimer appears, indicate that the content and scientific understanding are specific to the time of publication. This means that while some scientific descriptions are still accurate, the terminology and interpretation of the developmental mechanisms reflect the understanding at the time of original publication and those of the preceding periods, these terms, interpretations and recommendations may not reflect our current scientific understanding.     (More? Embryology History | Historic Embryology Papers)

Appendix 1 | Appendix 2

Appendix 1 - Embryos In The Carnegie Collection

The Carnegie specimens of stages 2-23 are listed in the following tables.


In columns "Size" (stage 9 and later), E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion. In stages 16-18, an asterisk indicates a corrected or an estimated length. The grade refers to the total grade of the specimen and includes both its original quality and the condition of the mounted sections. The embedding medium was either paraffin (P) or a combination of celloidin and paraffin (C-P).


In stages 9-12 the number of paired somites is provided. In stage 18 the number of semicircular ducts (1-3) is given for each embryo, and also the length of the paramesonephric duct. Both of these serve as indices of relative level of development. In stages 19- 23 Streeter's point score, from which the position of a given Carnegie embryo within a stage can be determined, is included (see Introduction To Stages 19-23).


The donors of most of these valuable specimens were named by Streeter (1942-1951) and O'Rahilly (1973). All those of stages 2, 3, 5a, and 5b, most of those of stage 5c, and many of those of stages 6 and 7 were presented by Drs. Hertig and Rock, whose contribution has been of enormous value to early human embryology.

Carnegie Collection


The specimens in the excellent embryological collection of Professor E. Blechschmidt were assigned Carnegie Nos. 10315-10434 in 1972 because Professor Blechschmidt's wish was and is to have his collection combined with the Carnegie Collection. The Blechschmidt Collection is at present housed temporarily in the Department of Anatomy of Louisiana State University, New Orleans, and is under the care of Dr. Raymond F. Gasser. The collection has served as the basis of two important atlases (Blechschmidt, 1963, 1973), and the appropriate three-dimensional reconstructions are housed in the Anatomisches Institut der Universitat Gottingen. The staging of these embryos has not been completed and hence they are not included in the following lists.

Blechschmidt Collection

Stage 2

Serial No. Normality No. of Blastomeres Size of Fixed Specimen (µm) Fixative Embedding Medium Thinness (µm) Stain Year Notes
8190 abnormal 9 160x104 Alc. & Bouin C-P 6 Iron h., or G 1943 Hertig et al. (1954)
8260 in vitro 2 50x75 Bouin C-P 8 (Stain - Haematoxylin Eosin) 1944 Menkin and Rock (1948)
8450 abnormal 8 100x96 Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1947 Hertig et al. (1954)
8452 abnormal 12 110x93 Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1946 Hertig et al. (1954)
8500.1 in vitro 3 50x86 Bouin C-P 8 (Stain - Haematoxylin Eosin). 1947 Menkin and Rock (1948)
8630 abnormal 5 104x94 Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1948 Hertig et al. (1954)
8698 normal 2 122x88 Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1949 Hertig et al. (1954)
8904 normal 12 115 Bouin Specimen lost 1951 Hertig et al. (1954)

1987 - Stage 2 | Carnegie stage 2

Carnegie Collection - Stage 2 
Serial No. Normality No. of Blastomeres Size of Fixed Specimen (µm) Fixative Embedding Medium Thinness (µm) Stain Year Notes
8190 abnormal 9 160x104 Alc. & Bouin C-P 6 Iron h., or G 1943 Hertig et al. (1954)
8260 in vitro 2 50x75 Bouin C-P 8 (Stain - Haematoxylin Eosin) 1944 Menkin and Rock (1948)
8450 abnormal 8 100x96 Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1947 Hertig et al. (1954)
8452 abnormal 12 110x93 Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1946 Hertig et al. (1954)
8500.1 in vitro 3 50x86 Bouin C-P 8 (Stain - Haematoxylin Eosin) 1947 Menkin and Rock (1948)
8630 abnormal 5 104x94 Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1948 Hertig et al. (1954)
8698 normal 2 122x88 Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1949 Hertig et al. (1954)
8904 normal 12 115 Specimen lost 1951 Hertig et al. (1954)
Abbreviations
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • ? - unknown or not determined.

Stage 3

Serial No. Normality No. of Blastomeres Size of Fixed Specimen (µm) Fixative Embedding Medium Thinness (µm) Stain Year Notes
8663 normal 107 103x80 Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1949 Hertig et al. (1954)
8794 normal 58 108x86 Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1950 Hertig et al. (1954)
Carnegie Collection - Stage 3 
Serial No. Normality No. of Blastomeres Size of Fixed Specimen (µm) Fixative Embedding Medium Thinness (µm) Stain Year Notes
8663 normal 107 103x80 Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1949 Hertig etal.(1954)[1]
8794 normal 58 108x86 Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1950 Hertig etal.(1954)[1]
Abbreviations
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • ? - unknown or not determined.

1987 - Stage 3 | Carnegie stage 3

Stage 5

Stage 5a

Serial No. Grade Fixative Embedding Medium Thinness (µm) Stain Year Notes
8020 Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1942 Hertig and Rock (1945a)
8155 Exc. Bouin C-P 6 (Stain - Haematoxylin Eosin) 1943 Hertig and Rock (1949)
8225 Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1944 Hertig and Rock (1945b)


1987 - Stage 5a | 1987 - Stage 5 | Carnegie stage 5

Stage 5b

Serial No. Grade Fixative Embedding Medium Thinness (µm) Stain Year Notes
8004 Exc Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1942 Hertig and Rock (1945a)
8171 Exc Alc. C-P 6 (Stain - Haematoxylin Eosin) 1943 Hertig and Rock (1949)
8215 Exc Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1944 Hertig and Rock (1945c)
9350 Exc Bouin ? ? (Stain - Haematoxylin Eosin) 1955 Heuser (1956)


1987 - Stage 5b | 1987 - Stage 5 | Carnegie stage 5

Stage 5c

Serial No. Grade Fixative Embedding Medium Thinness (µm) Stain Year Notes
4900 Poor p P 10 p 1925 Incomplete. Streeter (1926)
7699 Exc. Bouin C-P 6 (Stain - Haematoxylin Eosin) 1939 Hertigand Rock (1941)
7700 Exc. Bouin C-P 6 (Stain - Haematoxylin Eosin) 1938 Hertigand Rock (1941)
7770 Exc. Bouin C-P 6 & 10 (Stain - Haematoxylin Eosin) 1940 Abnormal
7771 Exc. Bouin C-P 10 (Stain - Haematoxylin Eosin) 1940 Abnormal
7950 Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1941 Hertig and Rock (1944)
8000 Poor Alc. & Bouin C-P 8 (Stain - Haematoxylin Eosin) 1942 Abnormal
8139 Exc. ? C-P 6 (Stain - Haematoxylin Eosin) 1943 Incomplete. Marchetti (1945)
8299 Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1945 Abnormal
8329 Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1945 Abnormal
8330 Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1945
8370 Poor Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1946 Abnormal
8558 Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1947

1987 - Stage 5c | 1987 - Stage 5 | Carnegie stage 5


Carnegie Collection - Stage 5 
Serial No. Stage Grade Fixative Embedding Medium Thinness (µm) Stain Year Notes
8020 5a Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1942 Hertig and Rock (1945a)[2]
8155 5a Exc. Bouin C-P 6 (Stain - Haematoxylin Eosin) 1943 Hertig and Rock (1949)[3]
8225 5a Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1944 Hertig and Rock (1945b)[4]
8004 5b Exc Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1942 Hertig and Rock (1945a)[2]
8171 5b Exc Alc. C-P 6 (Stain - Haematoxylin Eosin) 1943 Hertig and Rock (1949)[3]
8215 5b Exc Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1944 Hertig and Rock (1945c)[5]
9350 5b Exc Bouin ? ? (Stain - Haematoxylin Eosin) 1955 Heuser (1956)[6]
4900 5c Poor p P 10 p 1925 Incomplete. Streeter (1926)[7]
7699 5c Exc. Bouin C-P 6 (Stain - Haematoxylin Eosin) 1939 Hertig and Rock (1941)[8]
7700 5c Exc. Bouin C-P 6 (Stain - Haematoxylin Eosin) 1938 Hertig and Rock (1941)[8]
7771 5c Exc. Bouin C-P 10 (Stain - Haematoxylin Eosin) 1940 Abnormal
7950 5c Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1941 Hertig and Rock (1944)[9]
8000 5c Poor Alc. & Bouin C-P 8 (Stain - Haematoxylin Eosin) 1942 Abnormal
8139 5c Exc. ? C-P 6 (Stain - Haematoxylin Eosin) 1943 Incomplete. Marchetti (1945)[10]
8299 5c Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1945 Abnormal
8329 5c Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1945 Abnormal
8330 5c Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1945
8370 5c Poor Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin), phlox. 1946 Abnormal
8558 5c Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1947
Stage 5 was originally subdivided into 3 sequential parts a, b, c.

Abbreviations

  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • ? - unknown or not determined.
References
  1. 1.0 1.1 Hertig AT. Rock J. Adams EC. and Mulligan W.J. On the preimplantation stages of the human ovum: a description of four normal and four abnormal specimens ranging from the second to the fifth day of development. (1954) Carnegie Instn. Wash. Publ. 603, Contrib. Embryol., 35: 199-220.
  2. 2.0 2.1 Hertig AT. and Rock J. Two human ova of the pre-villous stage, having a developmental age of about seven and nine days respectively. (1945) Contrib. Embryol., Carnegie Inst. Wash. Publ. 557, 31: 65-84.
  3. 3.0 3.1 Hertig, A. T., and Rock, J. 1949. Two human ova of the pre-villous stage, having a developmental age of about eight and nine days respectively. Carnegie Instn. Wash. Publ. 583, Contrib. Embryol., 33, 169-186
  4. Hertig AT. and Rock J. On a normal human ovum not over 7.5 days of age. (1945) Anat. Rec. 91: 281.
  5. Hertig, A. T., and Rock J. 1945c. On a normal ovum of approximately 9 to 10 days of age. Anat. Rec, 91,281.
  6. Heuser, C. H. 1956. A human ovum with an estimated ovulation age of about nine days. Anat. Rec, 124, 459.
  7. Streeter, G. L. 1926. The "Miller" ovum—the youngest normal human embryo thus far known. Carnegie Instn. Wash. Publ 363, Contrib. Embryol., 18, 31-48.
  8. 8.0 8.1 Hertig, A. T., and Rock, J. 1941. Two human ova of the pre-villous stage, having an ovulation age of about eleven and twelve days respectively. Carnegie Instn. Wash. Publ. 525, Contrib. Embryol., 29, 127-156.
  9. Hertig, A. T., and Rock, J. 1944. On the development of the early human ovum, with special reference to the trophoblast of the previllous stage: a description of a normal and 5 pathologic human ova. Amer. J. Obstet Gynecol, 47, 149-184.
  10. Marchetti AA. A pre-villous human ovum accidentally recovered from a curettage specimen. (1945) Contrib. Embryol., Carnegie Inst. Wash. Publ. 557, 31: 107-115.


Stage 6

Serial No. Grade Fixative Embedding Medium Thinness (µm) Stain Year Notes
6026 Poor ? ? 6? H_&E 1929 Lockyer embryo. Abnormal. Ramsey (1937)
6734 Poor Zenker—acetic P 10 H.&E. 1934 Yale embryo. Ramsey (1938)
6900 Poor Formol P 15 H.&E. 1940 Linzenmeier (1914)
7634 Poor Formol P 10 H.&E. etc. 1940 Torpin embryo. Kraflca (1941)
7762 Good Zenker—formol P 8 ? 1940 Wilson (1945)
7800 Exc. ? C—P 8 H.&E. 1940 Abnormal
7801 Exc. Bouin C—P 8 H.&E. 1940 Heuser et al. (1945)
7850 Exc. Ale. & Bouin C—P 6 H.&E. 1940 Abnormal
8290 Exc. Bouin C—P 8 H. phlox. 1944
8360 Exc. Ale. & Bouin C—P 6 H&E., phlox. 1944
8362 Poor p C—P 6 H&E., phlox 1944
8672 Exc. Ale. & Bouin C—P 6 H&E. 1949
8819 Exc. Formol—chrom. subl. C—P 8 H.&E. 1951 Edwards—Jones—Brewer (H.I496). Brewer (1938)
8905 Poor Ale. p 6 H&E., phlox. 1951 Abnormal
8910 Good Formol C—P 8 H&E., phlox. 1951
9222 Good Bouin C—P 6 & 10 Azan 1954 Abnormal. Possibly stage 7
9250 Exc. Bouin p 8 H.&E. 1954
9595 Poor p p 8 H.&E. 1958
10003 Good Bouin p 5 Various 1963
Carnegie Collection - Stage 6 
Serial No. Grade Fixative Embedding Medium Thinness (µm) Stain Year Notes
6026 Poor ? ? 6? (Stain - Haematoxylin Eosin) 1929 Lockyer embryo. Abnormal. Ramsey (1937)
6734 Poor Zenker—acetic P 10 (Stain - Haematoxylin Eosin) 1934 Yale embryo. Ramsey (1938)
6900 Poor Formol P 15 (Stain - Haematoxylin Eosin) 1940 Linzenmeier (1914)
7634 Poor Formol P 10 (Stain - Haematoxylin Eosin) etc. 1940 Torpin embryo. Kraflca (1941)
7762 Good Zenker—formol P 8 ? 1940 Wilson (1945)
7800 Exc. ? C—P 8 (Stain - Haematoxylin Eosin) 1940 Abnormal
7801 Exc. Bouin C—P 8 (Stain - Haematoxylin Eosin) 1940 Heuser et al. (1945)
7850 Exc. Alc. & Bouin C—P 6 (Stain - Haematoxylin Eosin) 1940 Abnormal
8290 Exc. Bouin C—P 8 H. phlox. 1944
8360 Exc. Alc. & Bouin C—P 6 (Stain - Haematoxylin Eosin), phlox. 1944
8362 Poor p C—P 6 (Stain - Haematoxylin Eosin), phlox 1944
8672 Exc. Ale. & Bouin C—P 6 (Stain - Haematoxylin Eosin) 1949
8819 Exc. Formol—chrom. subl. C—P 8 (Stain - Haematoxylin Eosin) 1951 Edwards-Jones-Brewer (H1496). Brewer (1937, 38)[1][2]
8905 Poor Ale. p 6 (Stain - Haematoxylin Eosin), phlox. 1951 Abnormal
8910 Good Formol C—P 8 (Stain - Haematoxylin Eosin), phlox. 1951
9222 Good Bouin C—P 6 & 10 Azan 1954 Abnormal. Possibly stage 7
9250 Exc. Bouin p 8 (Stain - Haematoxylin Eosin) 1954
9595 Poor p p 8 (Stain - Haematoxylin Eosin) 1958
10003 Good Bouin p 5 Various 1963
Abbreviations
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • ? - unknown or not determined.
References

1987 - Stage 6 | Carnegie stage 6

Stage 7

Serial No. Grade Fixative Embedding Medium Thinness (µm) Stain Year Notes
7802 Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1940 Heuser et al. (1945)
8206 Good p C-P 6 (Stain - Haematoxylin Eosin) 1943
8361 Good Bouin C-P 10 p 1946 Abnormal
8602 Exc. Alc. C-P 8 (Stain - Haematoxylin Eosin) 1948
8752 Exc. ? C-P 10 (Stain - Haematoxylin Eosin) 1950
8756 Exc. Formol C-P 10 (Stain - Haematoxylin Eosin) 1950
9217 Exc. p P 10 (Stain - Haematoxylin Eosin) 1954

1987 - Stage 7 | Carnegie stage 7

Carnegie Collection - Stage 7 
Serial No. Grade Fixative Embedding Medium Thinness (µm) Stain Year Notes
7802 Exc. Alc. & Bouin C-P 6 (Stain - Haematoxylin Eosin) 1940 Heuser et al. (1945)
8206 Good p C-P 6 (Stain - Haematoxylin Eosin) 1943
8361 Good Bouin C-P 10 p 1946 Abnormal
8602 Exc. Alc. C-P 8 (Stain - Haematoxylin Eosin) 1948
8752 Exc. ? C-P 10 (Stain - Haematoxylin Eosin) 1950
8755 Exc. Formol C-P 10 (Stain - Haematoxylin Eosin) 1950
9217 Exc. p P 10 (Stain - Haematoxylin Eosin) 1954
Abbreviations
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • ? - unknown or not determined.


Stage 8

Serial No. Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
1399 Poor Formol P Trans. 10 (Stain - Haematoxylin Eosin) etc. 1916 "Mateer embryo" described by Streeter (1920a)
3412 Poor Formol P Trans. 5-15 Al. coch. E. au., or. 1921
5960 Good Kaiserling P Trans. 5 Al. coch. & eosin 1929 Heuser (1932b)
6630 Poor Formol P Oblique 6 (Stain - Haematoxylin Eosin) 1932
6815 Poor Formol P Oblique 10 Al. coch., or. G 1933
7170a and b 7545 Poor Alc. C-P Trans. 6 (Stain - Haematoxylin Eosin) 1935 Twins
7568 Poor Formoi C-P Trans. 6 (Stain - Haematoxylin Eosin) 1938
7640 Good Formol & Bouin P Trans. 10 (Stain - Haematoxylin Eosin) 1939 George (1942)
7666 Exc. Formol-chrom. subl. C-P Trans. 6 (Stain - Haematoxylin Eosin) 1939 "H. 1515"
7701 Exc. ? C-P Trans. 8 (Stain - Haematoxylin Eosin) 1939
7822 Good Formoi C-P Trans. 10 (Stain - Haematoxylin Eosin) 1940
7949 Good Zenker p Sag. 10 (Stain - Haematoxylin Eosin) etc. 1941
7972 Good Alc. & Bouin C-P Sag. 6 (Stain - Haematoxylin Eosin) 1942
8255 Exc. Bouin C-P Sag. 8 (Stain - Haematoxylin Eosin), phlox. 1944 Slides showing embryo returned to Dr. Patten in 1962
8320 Good Formol C-P Sag. 8 (Stain - Haematoxylin Eosin), phlox. 1945
8352 Good Formol C-P Trans. 8 (Stain - Haematoxylin Eosin), phlox. 1946
8371 Poor Alc. & Bouin C-P Sag. 8 (Stain - Haematoxylin Eosin), phlox. 1946
8671 Exc. Alc. & Bouin C-P Sag. 6 (Stain - Haematoxylin Eosin), phlox. 1949
8725 Exc. Alc. & Bouin C-P Sag. 6 (Stain - Haematoxylin Eosin), phlox. 1949
8727 Exc. Alc. & Bouin C-P Trans. 8 (Stain - Haematoxylin Eosin), phlox. 1949 Germ disc folded, possibly double (Hertig, 1968, fig. 180)
8820 Good Zenker-formol ? Trans. 10 (Stain - Haematoxylin Eosin) 1951 "Jones-Brewer I" (H. 1459) described byJones and Brewer (1941)
9009a and b 9123 Good Formol C-P Sag. 6 (Stain - Haematoxylin Eosin) 1952 Twins described briefly by Heuser (1954)
9123 Good Formol C-P Sag. 6 (Stain - Haematoxylin Eosin) 1953
9251 Good ? C-P Sag. 10-12 Azan, H. & phlox. 1954
9286 Exc. Formol C-P Trans. 8 Azan 1955
10157 Exc. Formol C-P Trans. ? Cason 1967
10174 Exc. Bouin p Trans. 8 Cason 1967

1987 - Stage 8 | Carnegie stage 8

Carnegie Collection - Stage 8 
Serial No. Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
1399 Poor Formol P Transverse 10 (Stain - Haematoxylin Eosin) etc. 1916 "Mateer embryo" described by Streeter (1920a)[1]
3412 Poor Formol P Transverse 5-15 Al. coch. E. au., or. 1921
5960 Good Kaiserling P Transverse 5 Al. coch. & eosin 1929 Heuser (1932b)[2]
6630 Poor Formol P Oblique 6 (Stain - Haematoxylin Eosin) 1932
6815 Poor Formol P Oblique 10 Al. coch., or. G 1933
7170a and b 7545 Poor Alc. C-P Transverse 6 (Stain - Haematoxylin Eosin) 1935 Twins
7568 Poor Formoi C-P Transverse 6 (Stain - Haematoxylin Eosin) 1938
7640 Good Formol & Bouin P Transverse 10 (Stain - Haematoxylin Eosin) 1939 George (1942)[3]
7666 Exc. Formol-chrom. subl. C-P Transverse 6 (Stain - Haematoxylin Eosin) 1939 "H. 1515"
7701 Exc. ? C-P Transverse 8 (Stain - Haematoxylin Eosin) 1939
7822 Good Formoi C-P Transverse 10 (Stain - Haematoxylin Eosin) 1940
7949 Good Zenker p Sagittal 10 (Stain - Haematoxylin Eosin) etc. 1941
7972 Good Alc. & Bouin C-P Sagittal 6 (Stain - Haematoxylin Eosin) 1942
8255 Exc. Bouin C-P Sagittal 8 (Stain - Haematoxylin Eosin), phlox. 1944 Slides showing embryo returned to Dr. Patten in 1962
8320 Good Formol C-P Sagittal 8 (Stain - Haematoxylin Eosin), phlox. 1945
8352 Good Formol C-P Transverse 8 (Stain - Haematoxylin Eosin), phlox. 1946
8371 Poor Alc. & Bouin C-P Sagittal 8 (Stain - Haematoxylin Eosin), phlox. 1946
8671 Exc. Alc. & Bouin C-P Sagittal 6 (Stain - Haematoxylin Eosin), phlox. 1949
8725 Exc. Alc. & Bouin C-P Sagittal 6 (Stain - Haematoxylin Eosin), phlox. 1949 Preparation method described by Heard (1957)[4]
8727 Exc. Alc. & Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin), phlox. 1949 Germ disc folded, possibly double (Hertig, 1968, fig. 180)[5]
8820 Good Zenker-formol ? Transverse 10 (Stain - Haematoxylin Eosin) 1951 "Jones-Brewer I" (H. 1459) described by Jones and Brewer (1941)[6]
9009a and b 9123 Good Formol C-P Sagittal 6 (Stain - Haematoxylin Eosin) 1952 Twins described briefly by Heuser (1954)[7]
8371 Good Formol C-P Sagittal 6 (Stain - Haematoxylin Eosin) 1953
9251 Good ? C-P Sagittal 10-12 Azan, H. & phlox. 1954
9286 Exc. Formol C-P Transverse 8 Azan 1955
10157 Exc. Formol C-P Transverse ? Cason 1967
10174 Exc. Bouin p Transverse 8 Cason 1967
Abbreviations
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • ? - unknown or not determined.
References
  1. Streeter GL. A human embryo (Mateer) of the pre-somite period. (1920) Contrib. Embryol., Carnegie Inst. Wash. Publ. 272, 9: 389-424.
  2. Heuser, C. H. 1932b. A presomite human embryo with a definite chorda canal. Carnegie Instn. Wash. Publ. 433, Contrib. Embryol., 23, 251-267.
  3. George, W. C. 1942. A presomite human embryo with chorda canal and prochordal plate. Carnegie Instn. Wash. Publ. 541, Contrib. Embryol., 30, 1-7.
  4. Heard OO. Methods used by C. H. Heuser in preparing and sectioning early embryos. (1957.) Carnegie Instn. Wash. Publ. 611, Contrib. Embryol., 36, 1-18.
  5. Hertig, A. T. 1968. Human Tropboblast. Thomas, Springfield, Illinois.
  6. Jones HO. and Brewer JI. A human embryo in the primitive-streak stage (Jones-Brewer ovum I). (1941) Carnegie Instn. Wash. Publ. 525, Contrib. Embryol., 29: 157-165.
  7. Heuser, C. H. 1954. Monozygotic twin human embryos with an estimated ovulation age of 17 days. Anat. Rec, 118, 310.

Stage 9

Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
1878 2-3 E., 1.38
Ch., 12X10.5X7.5
Good Formol P Cor. 10 (Stain - Haematoxylin Eosin) 1917 Described by Ingalls (1920)
5080 1 E., 1.5
Ch., 14.5
Poor Formol P Trans. 10 Al. coch. 1926 Studied by Davis (1927)
7650 2-3 E., 2-3 Good Alc & Bouirl C-P Trans. 6 (Stain - Haematoxylin Eosin) 1939 Said to be female (Park, 1957)

1987 - Stage 9 | Carnegie stage 9

Carnegie Collection - Stage 9 
Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
1878 2-3 Embryo, 1.38
Ch., 12x10.5x7.5
Good Formalin P Coronal 10 (Stain - Haematoxylin Eosin) 1917 Described by Ingalls (1920).[1]
5080 1 Embryo, 1.5
Ch., 14.5
Poor Formalin P Transverse 10 Al. coch. 1926 Studied by Davis (1927).[2]
7650 2-3 Embryo, 2-3 Good Alc & Bouin C-P Transverse 6 (Stain - Haematoxylin Eosin) 1939 Said to be female[3]
Abbreviations
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • ? - unknown or not determined.
References
  1. Ingalls, N.W. 1920. A human embryo at the beginning of segmentation, with special reference to the vascular system. Carnegie Instn. Wash. Publ. 274, Contrib. Embryol., 11, 61-90.
  2. Davis, C. L. 1927. Development of the human heart from its first appearance to the stage found in embryos of twenty paired somites. Carnegie Instn. Wash. Publ 380, Contrib. Embryol., 19, 245-284.
  3. PARK WW. (1957). The occurrence of sex chromatin in early human and macaque embryos. J. Anat. , 91, 369-73. PMID: 13448995

Stage 10

All sections at stage 10 are transverse

Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Thinness (µm) Stain Year Notes
391 8 E, 2 Ch., 14 Good Formol P 10 Al. coch. 1907 Monograph by Dandy (1910)
1201 7 E,2 Ch.. 144 Good Formol P 8 H. & or. G. 1915 Univ. Chicago No. H 87
2795 4-5 E,2 Poor Alc. P 6 Alcoch,or.G. 1919
3707 12 E, 1 5 Good Formol P 12.5 I. H. 1921 Univ. Calif. No. H 19"7
3709 4 E. 1.4 Ch.. 14.8 Poor Formol P 10 Erythrosin 1921 Univ. Chicago No H 279
3710 12 E., 3.6 Ch., 19.0 Good Formol C-P 10 H. & or. G. 1921 Univ. Chicago No. H 392
4216 8 E, 2 Ch, 9.8 Good Formol P 15 ? 1923 Monograph by Payne (1925)
5074 10 E., 3.3 Ch., 10.8 Exc. Bouin P 10 Al. coch. 1925 Univ. Rochester No. H 10. Monograph by Corner (1929)
6330 7 E, 2.83 Good P 5 Ehr. H. 1931 Univ. Chicago No. H 1404
6740 12 E., 2.2 Good p C-P 8 ? 1933 Litzenberg embryo. Studied by Boyden (1940)
7251 8 E., 1.27 Good Formol C-P 10 (Stain - Haematoxylin Eosin) 1941 "Singapore embryo." Univ. Cambridge No. H 98. Studied by Wilson (1914)
8244 6 E., 1.55 Ch, 8,5 Good Alc. C-P 8 (Stain - Haematoxylin Eosin) phlox. 1944
8970 12 Ch, ca. 8 Good Zenker P 5 Various, chiefly carmine 1952 Univ. Chicago. No. H 637. Dicephaly

1987 - Stage 10 | Carnegie stage 10

Carnegie Collection - Stage 10 
Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Thinness (µm) Stain Year Notes
391 8 E, 2 Ch., 14 Good Formalin P 10 Al. coch. 1907 Monograph by Dandy (1910)[1]
1201 7 E,2 Ch.. 144 Good Formalin P 8 H. & or. G. 1915 Univ. Chicago No. H 87
2795 4-5 E,2 Poor Alc. P 6 Al coch,or.G. 1919
3707 12 E, 1 5 Good Formalin P 12.5 I. H. 1921 Univ. Calif. No. H 197
3709 4 E. 1.4 Ch.. 14.8 Poor Formalin P 10 Erythrosin 1921 Univ. Chicago No H 279
3710 12 E., 3.6 Ch., 19.0 Good Formalin C-P 10 H. & or. G. 1921 Univ. Chicago No. H 392
4216 8 E, 2 Ch, 9.8 Good Formalin P 15 ? 1923 Monograph by Payne (1925)[2]
5074 10 E., 3.3 Ch., 10.8 Exc. Bouin P 10 Al. coch. 1925 Univ. Rochester No. H 10. Monograph by Corner (1929)[3]
6330 7 E, 2.83 Good P 5 Ehr. H. 1931 Univ. Chicago No. H 1404
6740 12 E., 2.2 Good p C-P 8 ? 1933 Litzenberg embryo. Studied by Boyden (1940)
7251 8 E., 1.27 Good Formalin C-P 10 (Stain - Haematoxylin Eosin) 1941 "Singapore embryo." Univ. Cambridge No. H 98.
Studied by Wilson (1914)[4]
8244 6 E., 1.55 Ch, 8,5 Good Alc. C-P 8 (Stain - Haematoxylin Eosin) phlox. 1944
9870 12 Ch, ca. 8 Good Zenker P 5 Various, chiefly carmine 1952 Univ. Chicago. No. H 637. Dicephaly
Abbreviations
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • ? - unknown or not determined.
  • All Carnegie stage 10 embryos are cut in Transverse plane.

Stage 11

Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
12 14 E, 2.1 Ch, 13 Poor P Trans. 10 Al. carm. 1893
164 18 E, 3.5 Ch, 14 Good Formol P Trans. 20 Al. carm. 1913
318 13/14 E, 2.5 Ch, 16 Good P Trans. 25 Al. carm. 1905
470 17 E, 4.3 Ch, 16 Good Formol P Trans. 10 Al. carm. 1910
779 14 E, 2.75 Good C Trans. 15 Al. coch. 1913 Complete dysraphia. Studied by Dekaban and Bartelmez (1964)
1182b E, 3 Ch, 15x12x5 Good Formol ? Trans. 20 Al. carm. 1915
2053 20 E, 3.1 Ch, 12 Exc. Formol P Trans. 10 Al. coch. 1918 Most advanced in group. Monograph by Davis (1923). Ag added to slide 2
4315 17 E, 4.7 Ch, 23x10.4X11 Exc. ? C-P Trans. 10 I.H. & E. 1923 Univ. Chicago No. 951. Studied by Wen (1928)
4529 14 E, 2.4 Ch, 21 Exc. Formol P Trans. 10 Al. coch, or. G. 1924 Monograph by Heuser (1930)
4783 13 E, 2.3 Fair ? ? Trans. 5 I.H. 1924 Monograph by Wallin (1913)
4877 13 E, 2 Ch, 15 Good Formol P Trans. 15 Al. coch. 1925
5072 17 E, 3 Good Formol P Trans. 10 (Stain - Haematoxylin Eosin) 1925 Tubal. Type specimen. Monograph by Atwell (1930)
6050 19/21 E.,3 Ch, 10 Good Formol C-P Cor. 10 Al. coch. 1930 Advanced
6344 13 E, 2.5 Ch, 17 Exc. Formol C-P Trans 6 Al. coch. 1931 Least advanced in group
6784 17 E, 5 Ch, 16 Exc. Formol C-P Trans 6 I.H, or. G. 1933
7358 16 E, ? Ch, 15 Poor Alc, formol p Oblique 25 (Stain - Haematoxylin Eosin) 1936
7611 16 E., 2.4 Ch., 12 Exc. Bouin C-P Trans 8 (Stain - Haematoxylin Eosin) 1938
7665 19 E., 4.36 Exc. ? C-P Trans 6 1939 Univ. Chicago No. H 1516
7702 17 E, 3.7 Ch., 14 Good Formol C-P Trans 10 Al. coch. 1940 Returned to B M Patten
7851 13 E., 4.3 Ch, 18 Exc. Formol C-P Trans 8 (Stain - Haematoxylin Eosin) 1940 Slightly injured
8005 16/17 E, 3 Exc. Bouin C-P Trans 8 (Stain - Haematoxylin Eosin) 1942 Tubal.
8116 17 E, 14 Ch.. 17 Good Formol p Sag. 8 Azan 1953
8962 15 E, 1.55 Good ? * Sag. ? ? 1952 Tubal. I'ntv Chicago No H 810
Carnegie Collection - Stage 11 
Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
12 14 E, 2.1 Ch, 13 Poor P Transverse 10 Al. carm. 1893
164 18 E, 3.5 Ch, 14 Good Formalin P Transverse 20 Al. carm. 1913
318 13/14 E, 2.5 Ch, 16 Good P Transverse 25 Al. carm. 1905
470 17 E, 4.3 Ch, 16 Good Formalin P Transverse 10 Al. carm. . 1910
779 14 E, 2.75 Good C Transverse 15 Al. coch. 1913 Dysraphism. Noted by Dekaban (1964)[5]
1182b E, 3 Ch, 15x12x5 Good Formalin ? Transverse 20 Al. carm. 1915
2053 20 E, 3.1 Ch, 12 Exc. Formalin P Transverse 10 Al. coch. 1918 Most advanced in group. Ag added to slide 2
Monographs by Davis (1923)[6] and Congdon (1922)[7]
4315 17 E, 4.7 Ch, 23x10.4X11 Excellent ? C-P Transverse 10 I.H. & E. 1923 Univ. Chicago No. 951. Wen (1928)[8]
4529 14 E, 2.4 Ch, 21 Excellent Formalin P Transverse 10 Al. coch, or. G. 1924 Heuser (1930)[9]
4783 13 E, 2.3 Fair ? ? Transverse 5 I.H. 1924 Wallin (1913)[10]
4877 13 E, 2 Ch, 15 Good Formalin P Transverse 15 Al. coch. 1925
5072 17 E, 3 Good Formalin P Transverse 10 (Stain - Haematoxylin Eosin) 1925 Tubal Type specimen. Atwell (1930)[11]
6050 19/21 E.,3 Ch, 10 Good Formalin C-P Coronal 10 Al. coch. 1930 Advanced
6344 13 E, 2.5 Ch, 17 Excellent Formalin C-P Transverse 6 Al. coch. 1931 Least advanced in group
6784 17 E, 5 Ch, 16 Excellent Formalin C-P Transverse 6 I.H, or. G. 1933
7358 16 E, ? Ch, 15 Poor Alc, formol p Oblique 25 (Stain - Haematoxylin Eosin) 1936
7611 16 E., 2.4 Ch., 12 Excellent Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1938
7665 19 E., 4.36 Excellent ? C-P Transverse 6 1939 Univ. Chicago No. H 1516
7702 17 E, 3.7 Ch., 14 Good Formalin C-P Transverse 10 Al. coch. 1940 Returned to B M Patten
7851 13 E., 4.3 Ch, 18 Excellent Formalin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1940 Slightly injured
8005 16/17 E, 3 Excellent Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1942 Tubal
8116 17 E, 14 Ch.. 17 Good Formalin p Sagittal 8 Azan 1953
8962 15 E, 1.55 Good ? * Sagittal ? ? 1952 Tubal Univ. Chicago No. H 810
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • ? - unknown or not determined.
References
  1. Dandy WE. A human embryo with seven pairs of somites measuring about 2 mm in length. (1910) Amer. J Anat. 10: 85-109.
  2. Payne, F. 1925. General description of a 7-somite human embryo. Carnegie Instn. Wash. Publ. 361, Contrib. Embryol., 16,115-124.
  3. Corner GW. A well-preserved human embryo of 10 somites. (1929) Contrib. Embryol., Carnegie Inst. Wash. Publ. 394, 20:81-102.
  4. Wilson JT. Observations upon young human embryos. (1914) J Anat Physiol., 48(3): 315-51 PMID 17233002 PMC1288949
  5. Dekaban AS. and Bartelmez GW. Complete Dysraphism in 14 Somite Human Embryo. (1964) A Contribution To Normal And Abnormal Morphogenesis. Am. J. Anat. 115: 27-38. PMID 14199785
  6. Davis CL. Description of a human embryo having twenty paired somites. (1923) Carnegie Instn. Wash. Publ. 332, Contrib. Embryol., 15: 1-51.
  7. Congdon ED. Transformation of the aortic-arch system during the development of the human embryo. (1922) Contrib. Embryol., Carnegie Inst. Wash. Publ 277, 14:47-110.
  8. Wen IC. The anatomy of human embryos with seventeen to twenty-three pairs of somites (1928) J. Comp. Neural., 45: 301-376.
  9. Heuser CH. A human embryo with 14 pairs of somites. (1930) Carnegie Instn. Wash. Publ. 414, Contrib. Embryol., Carnegie Inst. Wash. 22:135-153.
  10. Wallin IE. A human embryo of thirteen somites. (1913) Amer. J Anat. 15(3): 319-331.
  11. Atwell WJ. A human embryo with seventeen pairs of somites. (1930) Contrib. Embryol., Carnegie Inst. Wash. Publ. 407, 21: 1-24.

1987 - Stage 11 | Carnegie stage 11

Stage 12

Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
209 ca_24 EH3 Ch.,15 Poor Alc P Cor. 50 Al. coch. 1902
250 19? E , 2 (11, 10x9x9 Poor p ? Sag. 20 Al. coch. p
384 P E 2_5 Ch.,13 Poor Formol P Trans. 10 H.&E. 1907 Macerated. Narrow yolk stalk
486 21 E.,4 Ch., 22 Good Corros. acetic P Trans. 10 Al. Coch. 1911
1062 29 E.,4.5 Ch., 20 Good Formol P Trans. 20 Al. coch. 1915 Transitional to next stage
2197 ? E_,5_3 Ch., 19.5 Poor Formol P Trans. 10 Al. coch, or. G. 1918
4245-7 ca.24 E.,3.5 Ch., 24 Good Alc, formol P Trans. 10 Al. coch. 1923 Caudal neuropore widely open
4479 P E.,5 .8 Ch , 17 Poor Formol P Trans. 40 Al. coch. 1923 Macerated. Upper lirnb buds not visible
4736 26 E.,3.0 Ch.,20 Good Formol P Cor. 10 Al. coch. 1924 No upper limb buds. Caudal neuropore closed
4759 ? E.,4.5 Ch.,15 Good Formol P Trans. 15 H.&E. 1924 Neural tube folded
4784 23 E_,3 Good P P Trans. 10 p 1924
5035 25-28 E.,3.8 Ch.,18 Good Formol C-P Trans. 10 Al. coch. 1925
5048 ca_25 E_,3_5 Good Formol C-P Trans. 10 Al. coch. 1925 Tubal. Injured
5056 25 E,,3 Ch.,12 Good Formol P Trans. 10 Al. coch. 1925
5206 ? E.,4 Ch., 51x31x30 Poor ?? P Trans. 20 Al. coch. 1926 Tubal
5300 ? E., 4.5 Ch,16.5 Poor Formol P Trans. 20 Al. coch. 1926 Autopsy. Partly macerated
5923 28 E.,4 Ch.,15 Exc. Formol P Trans. 10 Al. coch. 1929
6097 25 E,3.4 Ch., 12.5 Exc. Formol C-P Trans. 10 Al. coch, eosin 1930 Tubal. Ag added to slides 1-3
6144 27 E. 3.3 Ch.,11 Good Lysol—Zenker C—P Trans. 10 Al. coch. 1930
6488 28 E, 32 Ch,22 Good Formol C—P Trans. 10 Al. coch. 1932
6937 26 E.,3 Ch , 12 Poor Formol C—P Cor. 10 I.H.,or.G. 1934 Tubal. Caudal neuropore closed
7724 ca.29 E,3.5 Ch.,18 Good Formol C—P Sag. 8 H.&E. 1940 Caudal end broken
7852 25 E , 3.7 Ch,26 Exc. Formol C—P Trans. 10 H.&E. 1940 Typical for stage 12
7999 ca.28 E,3.2 Ch , 15 Exc. Bruin C-P Trans. 10 H.&E. 1942 Caudal defect
8505a 24 Ch, 23.5 Exc. Formol C-P Trans. P H. Phlox. 1947
8505b 23 Ch,24 Exc. Formol C-P Sag. p Azan 1947 Twins
8941 28 E,4.9 Ch, 35 Exc. Zenker C-P Trans. 6 I.H. 1927 Univ. Chicago No. H 1261
8942 25 E, 38 Ch, 35 Exc. Formal-Zenker C-P Cor. 5 11-1. 1930 Univ. Chicago No. H 1382
8943 22 E. 3.9 Ch, 20.4 Exc. Formal-Zenker C-P Trans. 8 H.&E. 1934 Univ. Chicago No. H 1481
8944 25 E,4 Ch,,25 Exc. Formal-Zenker C-P Sag. 8 I.H. 1936 Univ. Chicago No. H 1514
8963 22 E, 3.8 Ch , 14.5 Fair Formol C-P Trans 10 I.H. 1928 Univ. Chicago. No. H 1093 Studied by Wen (1928)[1]
8964 23 E,2.8 Ch - 25 Poor Formol p Trans 8 I.H. 1928 Univ. Chicago No. H984 Studied by Wen (1928)[1]
9154 24 E, 5 4 Exc Formal C-P Trans I.H. & phlox. 1953
Carnegie Collection Embryos - Stage 12 
Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
209 ca_24 EH3 Ch.,15 Poor Alc P Coronal 50 Al. coch. 1902
250 19? E , 2 (11, 10x9x9 Poor p ? Sagittal 20 Al. coch. p
384 P E 2_5 Ch.,13 Poor Formalin P Transverse 10 H.&E. 1907 Macerated. Narrow yolk stalk
486 21 E.,4 Ch., 22 Good Corros. acetic P Transverse 10 Al. Coch. 1911
1062 29 E.,4.5 Ch., 20 Good Formalin P Transverse 20 Al. coch. 1915 Transitional to next stage
2197 ? E_,5_3 Ch., 19.5 Poor Formalin P Transverse 10 Al. coch, or. G. 1918
4245-7 ca.24 E.,3.5 Ch., 24 Good Alc, formol P Transverse 10 Al. coch. 1923 Caudal neuropore widely open
4479 P E.,5 .8 Ch , 17 Poor Formalin P Transverse 40 Al. coch. 1923 Macerated. Upper lirnb buds not visible
4736 26 E.,3.0 Ch.,20 Good Formalin P Coronal 10 Al. coch. 1924 No upper limb buds. Caudal neuropore closed
4759 ? E.,4.5 Ch.,15 Good Formalin P Transverse 15 (Stain - Haematoxylin Eosin) 1924 Neural tube folded
4784 23 E_,3 Good P P Transverse 10 p 1924
5035 25-28 E.,3.8 Ch.,18 Good Formalin C-P Transverse 10 Al. coch. 1925
5048 ca_25 E_,3_5 Good Formalin C-P Transverse 10 Al. coch. 1925 Tubal Injured
5056 25 E,,3 Ch.,12 Good Formalin P Transverse 10 Al. coch. 1925
5206 ? E.,4 Ch., 51x31x30 Poor ?? P Transverse 20 Al. coch. 1926 Tubal
5300 ? E., 4.5 Ch,16.5 Poor Formalin P Transverse 20 Al. coch. 1926 Autopsy. Partly macerated
5923 28 E.,4 Ch.,15 Exc. Formalin P Transverse 10 Al. coch. 1929
6097 25 E,3.4 Ch., 12.5 Exc. Formalin C-P Transverse 10 Al. coch, eosin 1930 Tubal Ag added to slides 1-3
6144 27 E. 3.3 Ch.,11 Good Lysol—Zenker C—P Transverse 10 Al. coch. 1930
6488 28 E, 32 Ch,22 Good Formalin C—P Transverse 10 Al. coch. 1932
6937 26 E.,3 Ch , 12 Poor Formalin C—P Coronal 10 I.H.,or.G. 1934 Tubal Caudal neuropore closed
7724 ca.29 E,3.5 Ch.,18 Good Formalin C—P Sagittal 8 (Stain - Haematoxylin Eosin) 1940 Caudal end broken
7852 25 E , 3.7 Ch,26 Exc. Formalin C—P Transverse 10 (Stain - Haematoxylin Eosin) 1940 Typical for stage 12
7999 ca.28 E,3.2 Ch , 15 Exc. Bouin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1942 Caudal defect
8505a 24 Ch, 23.5 Exc. Formalin C-P Transverse P H. Phlox. 1947
8505b 23 Ch,24 Exc. Formalin C-P Sagittal p Azan 1947 Twins
8941 28 E,4.9 Ch, 35 Exc. Zenker C-P Transverse 6 I.H. 1927 Univ. Chicago No. H 1261
8942 25 E, 38 Ch, 35 Exc. Formal-Zenker C-P Coronal 5 11-1. 1930 Univ. Chicago No. H 1382
8943 22 E. 3.9 Ch, 20.4 Exc. Formal-Zenker C-P Transverse 8 H.&E. 1934 Univ. Chicago No. H 1481
8944 25 E,4 Ch,,25 Exc. Formal-Zenker C-P Sagittal 8 I.H. 1936 Univ. Chicago No. H 1514
8963 22 E, 3.8 Ch , 14.5 Fair Formalin C-P Transverse 10 I.H. 1928 Univ. Chicago. No. H 1093 Studied by Wen (1928)[1]
8964 23 E,2.8 Ch - 25 Poor Formalin p Transverse 8 I.H. 1928 Univ_ Chicago No. H984 Studied by Wen (1928)[1]
9154 24 E, 5 4 Exc Formalin C-P Transverse I.H. & phlox. 1953
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • ? - unknown or not determined.
References

1987 - Stage 12 | Carnegie stage 12

Stage 13

Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
1 E.,4.5 Ch., 30x30 Poor Salicylic acid P Trans. 10 Hemat. 1887 Obtained by Mall while student
19 E., 5.5 Ch., 18x14 Poor p ? Trans. 20 Al. coch. 1895
87 E., 4 Ch., 24x16x9 Poor p P Trans. 20 Al. coch. 1896
76 E., 4.5 Ch., 22x20 Poor Alc. P Trans. 20 Al. coch. 1897
112 E., 4 Poor p P Sag. 10 Al. coch. p
116 E., 5 Poor p ? Sag. 10 Al. coch. 1898
148 E.,4.3 Ch., 17x14x10 Poor Alc. P Cor. 10 (Stain - Haematoxylin Eosin) 1899 Abnormal. Nasal discs fused
186 E.,3.5 Ch., 25x20x15 Poor Alc. P Trans. 20 Al. coch. 1901
239 E., 3.0 Poor Formol P Trans. 10 (Stain - Haematoxylin Eosin) 1903
248 E., 4.5 Ch., 30x23x15 Poor p ? Cor. 50 Al. coch. 1904
407 E.,4 Ch., 14x13X7 Poor Formol ? Trans. 40 Al. coch. 1907
463 E., 3.9 Ch., 17x12x7 Good Formol P Cor. 10 Al. coch. 1910
523 E., 5 Ch., 25x25x15 Fair Formol P Trans. p Al. coch. 1911
588 E., 4.0 Ch., 19x15x8 Good Corros. acetic P Cor. 15 (Stain - Haematoxylin Eosin) 1912 Advanced
786 E., 4.5 Ch., 19x10x10 Poor Alc. P Sag. 15 Al. coch. 1913
800 E., 6.0 Good Corros. acetic P Trans. 10 H 1913 Curettage. Anencephaly
808 E.,4.0 Poor Corros. acetic P Trans. 15 Al. coch. 1914 Tubal. Incomplete
826 E., 5.0 Ch., 13x13x9 Good Formol P Trans. 20 Al. coch. 1914 Shrunken
836 E.,4.0 Ch., 22x18x11 Exc. Corros. acetic P Trans. 15 Al. coch. 1914 Less advanced
963 E.,4.0 Ch., 23x18x16 Good Formol P Cor. 20 Al. coch. 1914
1075 E.,6.0 Ch., 46x32x20 Exc. Corros. acetic P Cor. 20 (Stain - Haematoxylin Eosin) or. G. 1915 Most advanced in group
3956 E., 4.0 Poor Formol P Trans. 20 Al. coch. 1922 Tubal. Incomplete
4046 E.,5 Ch., 22X20X20 Poor Formol P Trans. 50 Al. coch. 1922
5541 E., 6.0 Ch., 35x30x20 Good Formol P Trans. 10 Al. coch., eosin 1927
5682 E., 5.3 Ch., 29x25x13 Poor Formol P Cor. 20 Al. coch. 1928
5874 E., 4.8 Exc. Bouin P Trans. 10 (Stain - Haematoxylin Eosin) 1929 Hysterotomy. Bromides only
6032 E., 5.8 Ch., 30x24x13 Poor Formol P P ? p 1929 Not good enough to cut
6469 E., 5.0 Ch., 25x18x18 Poor Formol P P P P 1932 Fragmented on cutting. Not saved
6473 E., 5.0 Ch, 30X30X15 Exc. Formol C-P Cor. 6 Al. coch. 1932 Less advanced. Ag added
7433 E., 5.2 Ch., 15x13x13 Exc. Formol C-P Cor. 8 (Stain - Haematoxylin Eosin) 1937 Tubal
7618 E, 48 Ch, 18x15x15 Exc. Bouin C-P Cor. 10 (Stain - Haematoxylin Eosin) 1939 Hystereaomy. Advanced. Ag added
7669 E, 5.0 Ch., 23x16x14 Good Formol C-P Cor. 6 (Stain - Haematoxylin Eosin) 1939 Hysterectomy. Least advanced in group, Ag added
7889 E, 4.2 Exc. Bouin C-P Cor. 6 (Stain - Haematoxylin Eosin) 1941 Hysterectomy
8066 E,53 C h , 20 x 18 x I8 Exc. Bouin C-P Trans. 8 (Stain - Haematoxylin Eosin) 1942 Hysterectomy. Ag added to slide 2
8119 E., 5.3 Ch., 32x28x6.5 Exc. Bouin C-P Trans. 8 (Stain - Haematoxylin Eosin) 1943 Hysterectomy
8147 E., 5.2 Ch., 27x21x19 Poor Formol ? ? p p 1943 Tubal. Not cut
8239 E., 4.3 Exc. Bouin C-P Sag. 8 H. phlox. 1944
8372 E., 5.6 Exc. Alc.& Bouin P Trans. 10 Azan 1946
8581 E., 4.8 Good Kaiserling C-P Sag. 8 Azan 1948 Most-advanced third
8967 E., 5.7 Exc. Acetic Zenker C-P Trans. 6 (Stain - Haematoxylin Eosin) 1931 Head injured. Univ. Chicago No. H1426
9296 E,4.5 Exc. C-P Cor. 8 Azan 1955
9297 E., 4.5 Exc. C-P Sag. 8 Azan 1955
9697 E., 5.5 Bouin 1956 not cut
Carnegie Collection Embryos - Stage 13 
Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
1 E.,4.5 Ch., 30x30 Poor Salicylic acid P Transverse 10 Hemat. 1887 Obtained by Mall while student
19 E., 5.5 Ch., 18x14 Poor p ? Transverse 20 Al. coch. 1895
98 E., 4 Ch., 24x16x9 Poor p P Transverse 20 Al. coch. 1896
76 E., 4.5 Ch., 22x20 Poor Alc. P Transverse 20 Al. coch. 1897
112 E., 4 Poor p P Sagittal 10 Al. coch. p
116 E., 5 Poor p ? Sagittal 10 Al. coch. 1898
148 E.,4.3 Ch., 17x14x10 Poor Alc. P Coronal 10 (Stain - Haematoxylin Eosin) 1899 Abnormal. Nasal discs fused
186 E.,3.5 Ch., 25x20x15 Poor Alc. P Transverse 20 Al. coch. 1901
239 E., 3.0 Poor Formalin P Transverse 10 (Stain - Haematoxylin Eosin) 1903
248 E., 4.5 Ch., 30x23x15 Poor p ? Coronal 50 Al. coch. 1904
407 E.,4 Ch., 14x13X7 Poor Formalin ? Transverse 40 Al. coch. 1907
463 E., 3.9 Ch., 17x12x7 Good Formalin P Coronal 10 Al. coch. 1910
523 E., 5 Ch., 25x25x15 Fair Formalin P Transverse p Al. coch. 1911
588 E., 4.0 Ch., 19x15x8 Good Corros. acetic P Coronal 15 (Stain - Haematoxylin Eosin) 1912 Advanced
786 E., 4.5 Ch., 19x10x10 Poor Alc. P Sagittal 15 Al. coch. 1913
800 E., 6.0 Good Corros. acetic P Transverse 10 H 1913 Curettage. Anencephaly
808 E.,4.0 Poor Corros. acetic P Transverse 15 Al. coch. 1914 Tubal Incomplete
826 E., 5.0 Ch., 13x13x9 Good Formalin P Transverse 20 Al. coch. 1914 Shrunken
836 E.,4.0 Ch., 22x18x11 Exc. Corros. acetic P Transverse 15 Al. coch. 1914 Less advanced
963 E.,4.0 Ch., 23x18x16 Good Formalin P Coronal 20 Al. coch. 1914
1075 E.,6.0 Ch., 46x32x20 Exc. Corros. acetic P Coronal 20 (Stain - Haematoxylin Eosin) or. G. 1915 Most advanced in group
3956 E., 4.0 Poor Formalin P Transverse 20 Al. coch. 1922 Tubal Incomplete
4046 E.,5 Ch., 22x20x20 Poor Formalin P Transverse 50 Al. coch. 1922
5541 E., 6.0 Ch., 35x30x20 Good Formalin P Transverse 10 Al. coch., eosin 1927
5682 E., 5.3 Ch., 29x25x13 Poor Formalin P Coronal 20 Al. coch. 1928
5874 E., 4.8 Exc. Bouin P Transverse 10 (Stain - Haematoxylin Eosin) 1929 Hysterotomy. Bromides only
6032 E., 5.8 Ch., 30x24x13 Poor Formalin P P ? p 1929 Not good enough to cut
6469 E., 5.0 Ch., 25x18x18 Poor Formalin P P P P 1932 Fragmented on cutting. Not saved
6473 E., 5.0 Ch, 30x30x15 Exc. Formalin C-P Coronal 6 Al. coch. 1932 Less advanced. Ag added
7433 E., 5.2 Ch., 15x13x13 Exc. Formalin C-P Coronal 8 (Stain - Haematoxylin Eosin) 1937 Tubal
7618 E, 48 Ch, 18x15x15 Exc. Bouin C-P Coronal 10 (Stain - Haematoxylin Eosin) 1939 Hystereaomy. Advanced. Ag added
7669 E, 5.0 Ch., 23x16x14 Good Formalin C-P Coronal 6 (Stain - Haematoxylin Eosin) 1939 Hysterectomy. Least advanced in group, Ag added
7889 E, 4.2 Exc. Bouin C-P Coronal 6 (Stain - Haematoxylin Eosin) 1941 Hysterectomy
8066 E,53 Ch , 20x18xI8 Exc. Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1942 Hysterectomy. Ag added to slide 2
8119 E., 5.3 Ch., 32x28x6.5 Exc. Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1943 Hysterectomy
8147 E., 5.2 Ch., 27x21x19 Poor Formalin ? ? p p 1943 Tubal Not cut
8239 E., 4.3 Exc. Bouin C-P Sagittal 8 H. phlox. 1944
8372 E., 5.6 Exc. Alc.& Bouin P Transverse 10 Azan 1946
8581 E., 4.8 Good Kaiserling C-P Sagittal 8 Azan 1948 Most-advanced third
8967 E., 5.7 Exc. Acetic Zenker C-P Transverse 6 (Stain - Haematoxylin Eosin) 1931 Head injured. Univ. Chicago No. H1426
9296 E,4.5 Exc. C-P Coronal 8 Azan 1955
9297 E., 4.5 Exc. C-P Sagittal 8 Azan 1955
9697 E., 5.5 Bouin 1956 not cut
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • Stain -
  • ? - unknown or not determined.


Stage 14

Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
4 E.,7 Poor p P Trans. 10 Al. coch. 1892
18 E.,7 Ch., 18x18 Poor p P Trans. 20 Al. coch. 1895
80 E., 5.0 Ch., 24x18x8 Good Alc. P Trans. 20 Al. coch. 1897
187 E.,7 Ch., 35x30x25 Poor ? P Sag. 20 Al. coch. 1902
208 E.,7 Ch., 22x11Xx1 Poor ? P Sag. 20 Al. coch. 1902
245 E.,6 Ch., 13x12x10 Poor Formol, Zenker ? Trans. 5 (Stain - Haematoxylin Eosin) 1904
372 E.,7 Fair p P Trans. 10 H.-Congo red 1902
380 E,6 Ch., 20x20x14 Poor p P Sag. 20 (Stain - Haematoxylin Eosin) 1906
387 E.,7 Ch., 45x40x50 Good Formol P Trans. 20 (Stain - Haematoxylin Eosin) 1907
442 E.,6 Ch., 25x20 Poor Formol P Cor.? 50 Al. coch. 1908
552 E.,6 Ch, 40x28Xx8 Poor p P Sag. 40 Al. coch. 1911 Possible anencephaly
560 E., 7.0 Ch, 24x24 Poor Formol P Cor. 40 Al. coch. 1912
676 E., 6.0 Ch, 35x20x17 Good Carnoy P Tr.-Cor. 20 (Stain - Haematoxylin Eosin) 1913 Possible spina bifida
873 E,6.0 Ch., 35x28x16 Poor Formol P Sag. 20 Al. coch. 1914
988 E,6.0 Ch., 38x30x23 Good Formol-corros. acetic P Trans. 20 Al. coch. 1914
1380 E , 5.7 Ch, 36x24x24 Exc. Formol P Cor. 20 Al. coch. 1916
1620 E, 6.6 Ch, 35x30x8 Good Formol P Sag. 20 Al. coch. 1916
?? E, 6.68 Fair ? ? Trans. 6 Al. coch., or. G 1919
2841 E , sy Ch. 35x21 Good Alc. P Trans. 20 (Stain - Haematoxylin Eosin) or.G 1920
3360 E.6.0 Good Formol C Trans. 20 (Stain - Haematoxylin Eosin) or.G 1920 In mvomatous uterus. Advanced.
3805 E., 5.9 Exc. Bouin P Trans. 15 (Stain - Haematoxylin Eosin) 1921 Evans embryo No. 168. Serial bromides only
3960 E., 5.5 Good Formol C-P Cor. 20 Al. coch. 1922 Blood vessels naturally injected
4154 E, 6.8 Ch., 33x31x20 Poor Alc. C-P Trans. 8 (Stain - Haematoxylin Eosin) 1923
4245-6 E., 7.0 Good Formol P Trans. 15 Al. coch. 1923 Univ. Pennsylvania No. 40. Ag added
4629 E., 6.5 Ch.,32x23 Good Formol C-P Sag. 10 (Stain - Haematoxylin Eosin) 1924
4672 E,8.2 Ch., 40X34X25 Good Formol P Trans. 20 Al. coch. 1924 Advanced
4805 E., 7.3 Ch., 15X8X9 Good Formol C-P Trans. 10 (Stain - Haematoxylin Eosin) 1924 Tubal
5437 E., 7.0 Good Formol C-P Trans. 8 (Stain - Haematoxylin Eosin) 1927 Advanced
5654 E., 5.0 Ch., 30X23X17 Good Formol P Trans. 10 Al. coch., eosin 1928 Less advanced
5787 E., 6.8 Ch., 32x30x23 Good Formol P Sag. 10 Al. coch., eosin 1928
6428 E., 7.0 Ch., 30X28X25 Good Formol C-P Cor. 6, 10 Al. coch. 1931 Advanced
6500 E, 4.9* Good Souza? C-P Sag. 10 Al. coch. 1931 E. Leitz, Berlin
6502 E., 6.7* Exc. Souza? C-P Trans. 5, 10 (Stain - Haematoxylin Eosin) 1931 E. Leitz, Berlin. Ag added to slides 1-25
6503 E., 6.3* Exc. Souza? C-P Cor. 10 Al. coch. 1931 E. Leitz, Berlin
6739 E.,8 Poor Formol C-P Sag. 20 (Stain - Haematoxylin Eosin) 1933
6830 E,5.5 Ch., 47X23X15 Exc. Formol C-P Cor. 8 (Stain - Haematoxylin Eosin) 1933
6848 E., 7.8 Good Formol C-P Cor. 10 (Stain - Haematoxylin Eosin) 1934 Tubal
7324 E, 6.6 Ch., 17x13x10 Good Formol C-P Trans. 8 (Stain - Haematoxylin Eosin) 1936 Low implantation
7333 E, 6.3 Good Formol C-P Trans. 8 (Stain - Haematoxylin Eosin) 1936
7394 E, 7.2 Ch., 45X20X20 Exc. Formol C-P Trans. 8 (Stain - Haematoxylin Eosin) 1937
7400 E, 6.3 Ch., 35X25x20 Good Formol C-P Cor. 10 (Stain - Haematoxylin Eosin) 1937
7522 E., 7.7 Ch., 33x16x16 Good Formol C-P Trans. 8 (Stain - Haematoxylin Eosin) 1938 Natural blood injection
7598 E., 7.0 Ch., 30x30x25 Poor Alc. C-P Trans. 10 (Stain - Haematoxylin Eosin) 1938 Macerated
7667 E., 5 Ch., 16x14x12 Fair Formol P Trans. 8 (Stain - Haematoxylin Eosin), phlox. 1939
7829 E., 7.0 Exc. Bouin C-P Trans. 8 (Stain - Haematoxylin Eosin) 1940 Advanced
7870 E,7.2 Ch., 25x20x13 Exc. Bouin C-P Trans. 8 (Stain - Haematoxylin Eosin) 1941 On borderline of next stage. Ag added
8141 E,7.3 Ch.,33x28 Exc. C-P Cor. 8 (Stain - Haematoxylin Eosin) 1943 Shrinkage cracks in brain
8306 E.5.3 Ch., 27 Exc. Bouin C-P Trans. 10, 20 (Stain - Haematoxylin Eosin), phlox. 1945
8308 E, 5.85 Ch., 27x18x18 Exc. Formol & Bouin C-P Sag. 8 Azan 1945
8314 E,8 Ch.,23x22 Exc. Formol C-P Trans. 8 Azan 1945
8357 E., 6.5 Good Formol C-P Sag. 8 Azan 1946
8552 E,6.5 Exc. Alc. & Bouin C-P Trans. 8 Azan 1947
8999 E,6 Ch.,16x15 Exc. Alc. & Bouin C-P Sag. 8 Azan 1952
9695 E,8.5 ? 1955 Not cut
Carnegie Collection Embryos - Stage 14 
Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
4 E.,7 Poor p P Transverse 10 Al. coch. 1892
18 E.,7 Ch., 18x18 Poor p P Transverse 20 Al. coch. 1895
80 E., 5.0 Ch., 24x18x8 Good Alc. P Transverse 20 Al. coch. 1897
187 E.,7 Ch., 35x30x25 Poor ? P Sagittal 20 Al. coch. 1902
1208 E.,7 Ch., 22x11Xx1 Poor ? P Sagittal 20 Al. coch. 1902
245 E.,6 Ch., 13x12x10 Poor Formol, Zenker ? Transverse 5 (Stain - Haematoxylin Eosin) 1904
372 E.,7 Fair p P Transverse 10 H.-Congo red 1902
380 E,6 Ch., 20x20x14 Poor p P Sagittal 20 (Stain - Haematoxylin Eosin) 1906
387 E.,7 Ch., 45x40x50 Good Formalin P Transverse 20 (Stain - Haematoxylin Eosin) 1907
442 E.,6 Ch., 25x20 Poor Formalin P Coronal? 50 Al. coch. 1908
552 E.,6 Ch, 40x28Xx8 Poor p P Sagittal 40 Al. coch. 1911 Possible anencephaly
560 E., 7.0 Ch, 24x24 Poor Formalin P Coronal 40 Al. coch. 1912
676 E., 6.0 Ch, 35x20x17 Good Carnoy P Tr.-Coronal 20 (Stain - Haematoxylin Eosin) 1913 Possible spina bifida
873 E,6.0 Ch., 35x28x16 Poor Formalin P Sagittal 20 Al. coch. 1914
988 E,6.0 Ch., 38x30x23 Good Formol-corros. acetic P Transverse 20 Al. coch. 1914
1380 E , 5.7 Ch, 36x24x24 Exc. Formalin P Coronal 20 Al. coch. 1916
1620 E, 6.6 Ch, 35x30x8 Good Formalin P Sagittal 20 Al. coch. 1916
?? E, 6.68 Fair ? ? Transverse 6 Al. coch., or. G 1919
2841 E , sy Ch. 35x21 Good Alc. P Transverse 20 (Stain - Haematoxylin Eosin) or.G 1920
3360 E.6.0 Good Formalin C Transverse 20 (Stain - Haematoxylin Eosin) or.G 1920 In myomatous uterus. Advanced.
3805 E., 5.9 Exc. Bouin P Transverse 15 (Stain - Haematoxylin Eosin) 1921 Evans embryo No. 168. Serial bromides only
3960 E., 5.5 Good Formalin C-P Coronal 20 Al. coch. 1922 Blood vessels naturally injected
4154 E, 6.8 Ch., 33x31x20 Poor Alc. C-P Transverse 8 (Stain - Haematoxylin Eosin) 1923
4245-6 E., 7.0 Good Formalin P Transverse 15 Al. coch. 1923 Univ. Pennsylvania No. 40. Ag added
4692 E., 6.5 Ch., 32x23 Good Formalin C-P Sagittal 10 (Stain - Haematoxylin Eosin) 1924
4672 E,8.2 Ch., 40X34X25 Good Formalin P Transverse 20 Al. coch. 1924 Advanced
4805 E., 7.3 Ch., 15X8X9 Good Formalin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1924 Tubal
5437 E., 7.0 Good Formalin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1927 Advanced
5654 E., 5.0 Ch., 30x23x17 Good Formalin P Transverse 10 Al. coch., eosin 1928 Less advanced
5787 E., 6.8 Ch., 32x30x23 Good Formalin P Sagittal 10 Al. coch., eosin 1928
6428 E., 7.0 Ch., 30X28X25 Good Formalin C-P Coronal 6, 10 Al. coch. 1931 Advanced
6500 E, 4.9* Good Souza? C-P Sagittal 10 Al. coch. 1931 E. Leitz, Berlin
6502 E., 6.7* Exc. Souza? C-P Transverse 5, 10 (Stain - Haematoxylin Eosin) 1931 E. Leitz, Berlin. Ag added to slides 1-25
6503 E., 6.3* Exc. Souza? C-P Coronal 10 Al. coch. 1931 E. Leitz, Berlin
6739 E.,8 Poor Formalin C-P Sagittal 20 (Stain - Haematoxylin Eosin) 1933
6830 E,5.5 Ch., 47x23x15 Exc. Formalin C-P Coronal 8 (Stain - Haematoxylin Eosin) 1933
6848 E., 7.8 Good Formalin C-P Coronal 10 (Stain - Haematoxylin Eosin) 1934 Tubal
7324 E, 6.6 Ch., 17x13x10 Good Formalin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1936 Low implantation
7333 E, 6.3 Good Formalin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1936
7394 E, 7.2 Ch., 45x20x20 Exc. Formalin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1937
7400 E, 6.3 Ch., 35x25x20 Good Formalin C-P Coronal 10 (Stain - Haematoxylin Eosin) 1937
7522 E., 7.7 Ch., 33x16x16 Good Formalin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1938 Natural blood injection
7598 E., 7.0 Ch., 30x30x25 Poor Alc. C-P Transverse 10 (Stain - Haematoxylin Eosin) 1938 Macerated
7667 E., 5 Ch., 16x14x12 Fair Formalin P Transverse 8 (Stain - Haematoxylin Eosin), phlox. 1939
7829 E., 7.0 Exc. Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1940 Advanced
7870 E,7.2 Ch., 25x20x13 Exc. Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1941 On borderline of next stage. Ag added
8141 E,7.3 Ch.,33x28 Exc. C-P Coronal 8 (Stain - Haematoxylin Eosin) 1943 Shrinkage cracks in brain
8306 E.5.3 Ch., 27 Exc. Bouin C-P Transverse 10, 20 (Stain - Haematoxylin Eosin), phlox. 1945
8308 E, 5.85 Ch., 27x18x18 Exc. Formol & Bouin C-P Sagittal 8 Azan 1945
8314 E,8 Ch.,23x22 Exc. Formol C-P Transverse 8 Azan 1945
8357 E., 6.5 Good Formol C-P Sagittal 8 Azan 1946
8552 E,6.5 Exc. Alc. & Bouin C-P Transverse 8 Azan 1947
8999 E,6 Ch.,16x15 Exc. Alc. & Bouin C-P Sagittal 8 Azan 1952
9695 E,8.5 ? 1955 Not cut
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • Stain -
  • ? - unknown or not determined.

Stage 15

Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
2 E., 7.0 Ch., 25X25X25 Good Alc. P Trans. 15 Al. carm. 1888 Least-advanced third
88 E.,8 Ch., 30x28x15 Poor Alc. P Cor. P Al. coch. 1897
113 E.,8 241 E,6.0 Poor P p Sag. 10 Borax carmine ?
241 E,6.0 Good Formol P Trans. 10 H. & Congo red 1904
371 E,6.6 Good Formol P Sag. 10 Al. coch. 1913 Shrunken and cracked
389 E., 9 Poor p p Sag. 20 (Stain - Haematoxylin Eosin) 1907 Tubal
721 E., 9.0 Ch., 30x20x10 Exc. Zenker formol P Trans. 15 (Stain - Haematoxylin Eosin) 1913 Median in group
810 E., 7.0 Ch, 30x25x15 Good Alc. P Sag. 20 Al. coch 1913
855 E.,7.5 Poor Formol P Trans. 100 Al. coch. 1914 Pathological between limbs
1006 E,9.0 Ch., 37x26x22 Poor Formol P Cor. 20 (Stain - Haematoxylin Eosin) or. G. 1914 Operative. Most-advanced third
1091 E,7.2 Ch., 28x26x20 Poor P Cor. 20 Al. coch. 1915 Macerated
1354 E,7.8 Ch, 35x30x25 Good Formol P Sag. 20 Al. coch. 1916 Least-advanced third
1767 E , 11.0 Ch, 41 X23X5 Good Formol P Sag. 40 (Stain - Haematoxylin Eosin) or. G. 1917 Most-advanced third
2743 E., 7.2 Ch., 19xl8x 14 Poor Formol P Trans. 20 Al. coch. 1919 Macerated. Least-advanced third
3216 E, 6.5 Ch, 30x30x5 Good Formol P Trans. 20 Al. coch. 1920 Hysterectomy. Least-advanced third
3385 E,83 Ch., 25x20x16 Exc. Corros. acetic P Trans. 20 (Stain - Haematoxylin Eosin) or. G. 1921 Some sections lost. Most-advanced third. Ag added
3441 E,8.0 Ch., 25x24x20 Good Formol P Sag. 10 Al. coch. 1921
3512 E,8,5 Ch., 33x28x25 Good Formol P Trans. 10 Al. coch. 1921
3952 E,6,7 Ch., 30x25x15 Good Formol P Cor. 15 Al. coch. 1922 Median in group
4602 E,9.3 Ch,, 33x30x26 Good Formol P Sag. 15 Al. coch. 1924 Medical abortion
4782 E,9.0 Ch., I4xl3x 11 Poor Formol P Cor. 20 Al. coch. 1924
5772 E, 8 Poor ? P Cor. 15 Al. coch. eosin 1928
5?92 E, 3 Good Corros. acetic C-P Cor. 10 Al. coch. phlox 1929 Transitional to next stage
6223 E ? Poor Alc. C-P Sag. 8 Or. G. 1930 Fragmented sections. Not saved
65??
6???
6508
6595
????


Carnegie Collection Embryos - Stage 15 
Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
2 E., 7.0 Ch., 25x25x25 Good Alc. P Transverse 15 Al. carm. 1888 Least-advanced third
88 E.,8 Ch., 30x28x15 Poor Alc. P Coronal P Al. coch. 1897
113 E.,8 241 E,6.0 Poor P p Sagittal 10 Borax carmine ?
241 E,6.0 Good Formalin P Transverse 10 H. & Congo red 1904
371 E,6.6 Good Formalin P Sagittal 10 Al. coch. 1913 Shrunken and cracked
389 E., 9 Poor p p Sagittal 20 (Stain - Haematoxylin Eosin) 1907 Tubal
721 E., 9.0 Ch., 30x20x10 Exc. Zenker formol P Transverse 15 (Stain - Haematoxylin Eosin) 1913 Median in group
810 E., 7.0 Ch, 30x25x15 Good Alc. P Sagittal 20 Al. coch 1913
855 E.,7.5 Poor Formalin P Transverse 100 Al. coch. 1914 Pathological between limbs
1006 E,9.0 Ch., 37x26x22 Poor Formalin P Coronal 20 (Stain - Haematoxylin Eosin) or. G. 1914 Operative. Most-advanced third
1091 E,7.2 Ch., 28x26x20 Poor P P Coronal 20 Al. coch. 1915 Macerated
1354 E,7.8 Ch, 35x30x25 Good Formalin P Sagittal 20 Al. coch. 1916 Least-advanced third
1767 E , 11.0 Ch, 41x23x5 Good Formalin P Sagittal 40 (Stain - Haematoxylin Eosin) or. G. 1917 Most-advanced third
2743 E., 7.2 Ch., 19xl8x14 Poor Formalin P Transverse 20 Al. coch. 1919 Macerated. Least-advanced third
3216 E, 6.5 Ch, 30x30x5 Good Formalin P Transverse 20 Al. coch. 1920 Hysterectomy. Least-advanced third
3385 E,83 Ch., 25x20x16 Exc. Corros. acetic P Trans. 20 (Stain - Haematoxylin Eosin) or. G. 1921 Some sections lost. Most-advanced third. Ag added
3441 E,8.0 Ch., 25x24x20 Good Formalin P Sag. 10 Al. coch. 1921
3512 E,8,5 Ch., 33x28x25 Good Formalin P Trans. 10 Al. coch. 1921
3952 E,6,7 Ch., 30x25x15 Good Formalin P Cor. 15 Al. coch. 1922 Median in group
4602 E,9.3 Ch,, 33x30x26 Good Formalin P Sag. 15 Al. coch. 1924 Medical abortion
4782 E,9.0 Ch., I4xl3x11 Poor Formalin P Cor. 20 Al. coch. 1924
5772 E, 8 Poor ? P Cor. 15 Al. coch. eosin 1928
Template:CE5?92 E, 3 Good Corros. acetic C-P Cor. 10 Al. coch. phlox 1929 Transitional to next stage
6223 E ? Poor Alc. C-P Sag. 8 Or. G. 1930 Fragmented sections. Not saved
6504
6506
6508
6595
????
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • ? - unknown or not determined.

1987 - Stage 15 | Carnegie stage 15

Stage 16

Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
163 E., 9.0 Ch., 35x35x20 Good Formol P Transverse 20 Al. coch. 1899 Used by Bardeen and Lewis
221 E., 7.5 Ch., 40x33x33 Poor Formol P Sagittal 20 Al. coch. 1903 Macerated
383 E., 7.0 Ch., 15x15x15 Poor Formol P Trans. 10 Al. carm., H. & Congo red 1904
397 E., 8.0 Ch., 15x15x15 Poor Formol P Trans. 10 (Stain - Haematoxylin Eosin) 1907
422 E., 9.0 Ch., 30x30x30 Poor Alc. P Trans. 40 Al. coch. 1910 Tubal. Partly macerated
559 E., 8.6 Ch., 20x15x12 Good Formol P Trans. 20 H. & Congo red 1911 Cyclopia. Formerly listed as stage 17
589 E., 11 Ch., 30x13x13 Poor p P Sag. 50 Al. coch. 1912
617 E., 7.0 Ch., 18x14x12 Good Formol P Trans. 15 Al. coch. 1912 Median in group
636 E., 10 Ch.,28x28x22 Poor Formol P Trans. 50 Al. coch. 1913 Macerated
651f E., 7 Ch., 25x20x15 Poor p p p p p 1913 Spina bifida
675 E., 10 Ch., 50x30x25 Poor Formol P Sag. 100 Carmine 1915 Abnormal head and limbs
792 E., 8.0 Ch., 40x30x30 Good Formol P Trans. 20 Al. coch. 1913 Advanced
887 E, 9.0 Ch., 31x28x17 Good Formol P Trans. 40 Al. coch. 1914 Near next stage
1121 E., 11.8 Good Corros. acetic P Cor. 40 Al. coch. 1915 Operative. Median in group
1197 E., 10.0 Ch., 23x19x15 Good Formol C Sag. 20 (Stain - Haematoxylin Eosin), or. G. 1915 Advanced
1544 E., 7.2 Good Zenker P Sag. 20 Al. coch. 1916 Tubal. Mechanical injury
1836 E., 11.0 Good Formol P Trans. 20 (Stain - Haematoxylin Eosin) 1917 Most-advanced third
4677 E., 9.5 Ch., 48x36x30 Good Formol P Trans. 10 Al. coch. 1924 Median in group
5515 E., 12.0 Ch., 47x37x25 Good Formol C-P Trans. 10 (Stain - Haematoxylin Eosin) 1927 Near next stage
6054 E., 7,0 Ch., 21x17x12 Good Formol C-P Trans. 8 (Stain - Haematoxylin Eosin) 1930 Least-advanced third
6507 E., 9.0* Exc. Corros. acetic C-P Cor. 10 & 8 Al. coch. p Middle or most-advanced third
6509 E. 8.1* Exc. Corros. acetic C-P Cor. 10 Al. coch. p Least-adianced or middle third
6510 E., 10.1* Exc. Corros. acetic C-P Cor. 10 Al. coch. p Close to No. 6507. Ag added
6511 E, 8.1* Good Corros. acetic C-P Sag. 10 Al. coch., iron H. p Surface injured by fixative. Most-advanced third
6512 E., 7.0* Exc. Corros. acetic C-P Trans. 10 Al. coch. p Least-advanced third. Borderline
6513 E., 7.2* Good Corros. acetic C-P Cor. 10 Al. coch. p Surface injured by fixative. Least advanced in group
6514 E., 9 0* Poor Corros. acetic C-P Sag. 10 Al. coch. p Most-advanced third
6516 E., 10 5* Good Corros acetic C-P Sag. 8 Al. coch. p Most-advanced third. Double left kidney and ureter
6517 E., 10.5* Exc. Corros. acetic C-P Trans. 8 Al. coch. ? Close to No. 6516
6686 E., 11.0 Ch., l7x17xP Poor Formol C-P Cor. 20 Al. coch. 1933 Tubal. Partly macerated
6750 E., 10.0 Good Formol C-P Trans. 10 H. & phlox. 1933 Tubal. Advanced
6909 E., 11.0 Good Bouin C-P Cor. 10 (Stain - Haematoxylin Eosin) 1934 Tubal. Advanced
6931 E., 8.8 Ch., 3""x 33x16 Good Formol C-P Cor. 10 Al coch., phlox. 1934 Least-advanced third. Type specimen
6950 E.. 9 0 Ch., 3lx20x18 Good Formol C-P Trans. 10 (Stain - Haematoxylin Eosin) 1934 Tubal. Partly fragmented
7?15 E., 9.7 Exc Bouin C-P Cor. 10 H. & phlox 1935 Operative. Less advanced
7629 E., 11.5 Ch., 31x31 Good Formol C-P Cor. 10 Al. coch., phlox 1939 Hysterectomy. Most advanced in group
7804 E., 9.5 Ch., 26x21x16 Good Formol C-P Trans. 10 (Stain - Haematoxylin Eosin) 1940 Least-advanced third
7897 E., 12.2 Ch., 31x24x23 Good Formol C-P Trans. 10 (Stain - Haematoxylin Eosin) 1941 Tubal. Advanced
8098 E., 10.0 Ch., 30 Good Formol C-P Cor. 6 (Stain - Haematoxylin Eosin) 1942 Tubal. Median in group
8112 E., 10.9 Exc. Bouin C-P Cor. 8 (Stain - Haematoxylin Eosin) 1943 Most-advanced third
8179 E., 11.9 Ch., 23x18x17 Good Formol C-P Cor. 10 (Stain - Haematoxylin Eosin) 1943 Tubal
8436 E., 10.9 Ch., 13x15x1? Good Formol P Cor. 10 Azan 1946 Advanced
8692 E., 10 Good Bouin P Trans. 10 (Stain - Haematoxylin Eosin) 1949 Rubella. Medical abortion. Mechanically damaged
8697 E., 11.3 Poor Formol C-P Trans. 10 (Stain - Haematoxylin Eosin) 1949 Perhaps stage 17
8773 E, 11 Exc. Bouin P Cor. 10 Azan 1950
8971 E., 10 Ch., 20.5x14.5x13.7 Poor Formol Trans. 15 (Stain - Haematoxylin Eosin) 1932 Synophthalmia. Univ. Chicago No. H 1439
9055 E., ca. 10 Exc. Bouin P Trans. 20 Azan & Ag 1953 Damaged
9229 E, 9.5 Exc. Formol P Trans. 6 Ag & (Stain - Haematoxylin Eosin) 1954 Stage 15, 16, or 17? Mislaid


Carnegie Collection Embryos - Stage 16 
Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
163 E., 9.0 Ch., 35x35x20 Good Formol P Transverse 20 Al. coch. 1899 Used by Bardeen and Lewis
221 E., 7.5 Ch., 40x33x33 Poor Formalin P Sagittal 20 Al. coch. 1903 Macerated
383 E., 7.0 Ch., 15x15x15 Poor Formalin P Transverse 10 Al. carm., H. & Congo red 1904
397 E., 8.0 Ch., 15x15x15 Poor Formalin P Transverse 10 (Stain - Haematoxylin Eosin) 1907
422 E., 9.0 Ch., 30x30x30 Poor Alc. P Transverse 40 Al. coch. 1910 Tubal. Partly macerated
559 E., 8.6 Ch., 20x15x12 Good Formalin P Transverse 20 H. & Congo red 1911 Cyclopia. Formerly listed as stage 17
589 E., 11 Ch., 30x13x13 Poor p P Sagittal 50 Al. coch. 1912
617 E., 7.0 Ch., 18x14x12 Good Formalin P Transverse 15 Al. coch. 1912 Median in group
636 E., 10 Ch.,28x28x22 Poor Formalin P Transverse 50 Al. coch. 1913 Macerated
651f E., 7 Ch., 25x20x15 Poor p p p p p 1913 Spina bifida
675 E., 10 Ch., 50x30x25 Poor Formalin P Sagittal 100 Carmine 1915 Abnormal head and limbs
792 E., 8.0 Ch., 40x30x30 Good Formalin P Transverse 20 Al. coch. 1913 Advanced
887 E, 9.0 Ch., 31x28x17 Good Formalin P Transverse 40 Al. coch. 1914 Near next stage
1121 E., 11.8 Good Corros. acetic P Coronal 40 Al. coch. 1915 Operative. Median in group
1197 E., 10.0 Ch., 23x19x15 Good Formalin C Sagittal 20 (Stain - Haematoxylin Eosin), or. G. 1915 Advanced
1544 E., 7.2 Good Zenker P Sagittal 20 Al. coch. 1916 Tubal. Mechanical injury
1836 E., 11.0 Good Formalin P Transverse 20 (Stain - Haematoxylin Eosin) 1917 Most-advanced third
4677 E., 9.5 Ch., 48x36x30 Good Formalin P Transverse 10 Al. coch. 1924 Median in group
5515 E., 12.0 Ch., 47x37x25 Good Formalin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1927 Near next stage
6054 E., 7,0 Ch., 21x17x12 Good Formalin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1930 Least-advanced third
6507 E., 9.0* Excellent Corros. acetic C-P Coronal 10 & 8 Al. coch. p Middle or most-advanced third
6509 E. 8.1* Excellent Corros. acetic C-P Coronal 10 Al. coch. p Least-adianced or middle third
6510 E., 10.1* Excellent Corros. acetic C-P Coronal 10 Al. coch. p Close to No. 6507. Ag added
6511 E, 8.1* Good Corros. acetic C-P Sagittal 10 Al. coch., iron H. p Surface injured by fixative. Most-advanced third
6512 E., 7.0* Excellent Corros. acetic C-P Transverse 10 Al. coch. p Least-advanced third. Borderline
6513 E., 7.2* Good Corros. acetic C-P Coronal 10 Al. coch. p Surface injured by fixative. Least advanced in group
6514 E, 9 0* Poor Corros. acetic C-P Sagittal 10 Al. coch. p Most-advanced third
6516 E., 10 5* Good Corros acetic C-P Sagittal 8 Al. coch. p Most-advanced third. Double left kidney and ureter
6517 E., 10.5* Excellent Corros. acetic C-P Transverse 8 Al. coch. ? Close to No. 6516
6686 E., 11.0 Ch., l7x17xP Poor Formalin C-P Coronal 20 Al. coch. 1933 Tubal. Partly macerated
6750 E., 10.0 Good Formalin C-P Transverse 10 H. & phlox. 1933 Tubal. Advanced
6909 E., 11.0 Good Bouin C-P Coronal 10 (Stain - Haematoxylin Eosin) 1934 Tubal. Advanced
6931 E., 8.8 Ch., 3""x 33x16 Good Formalin C-P Coronal 10 Al coch., phlox. 1934 Least-advanced third. Type specimen
6950 E.. 9 0 Ch., 3lx20x18 Good Formalin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1934 Tubal. Partly fragmented
7?15 E., 9.7 Exc Bouin C-P Coronal 10 H. & phlox 1935 Operative. Less advanced
7629 E., 11.5 Ch., 31x31 Good Formalin C-P Coronal 10 Al. coch., phlox 1939 Hysterectomy. Most advanced in group
7804 E., 9.5 Ch., 26x21x16 Good Formalin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1940 Least-advanced third
7897 E., 12.2 Ch., 31x24x23 Good Formalin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1941 Tubal. Advanced
8098 E., 10.0 Ch., 30 Good Formalin C-P Coronal 6 (Stain - Haematoxylin Eosin) 1942 Tubal. Median in group
8112 E., 10.9 Excellent Bouin C-P Coronal 8 (Stain - Haematoxylin Eosin) 1943 Most-advanced third
8179 E., 11.9 Ch., 23x18x17 Good Formalin C-P Coronal 10 (Stain - Haematoxylin Eosin) 1943 Tubal
8436 E., 10.9 Ch., 13x15x1? Good Formalin P Coronal 10 Azan 1946 Advanced
8692 E., 10 Good Bouin P Transverse 10 (Stain - Haematoxylin Eosin) 1949 Rubella. Medical abortion. Mechanically damaged
8697 E., 11.3 Poor Formalin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1949 Perhaps stage 17
8773 E, 11 Excellent Bouin P Coronal 10 Azan 1950
8971 E., 10 Ch., 20.5x14.5x13.7 Poor Formalin Transverse 15 (Stain - Haematoxylin Eosin) 1932 Synophthalmia. Univ. Chicago No. H 1439
Template:CE9055 E., ca. 10 Excellent Bouin P Transverse 20 Azan & Ag 1953 Damaged
9229 E, 9.5 Excellent Formalin P Transverse 6 Ag & (Stain - Haematoxylin Eosin) 1954 Stage 15, 16, or 17? Mislaid
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin Bouin solution)
  • Stain -
  • ? - unknown or not determined.


1987 - Stage 16 | Carnegie stage 16

Stage 17

Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
353 E, 11.0 Ch., 40x35x20 Good Formol P Cor. 10 H.&E. 1906 Very advanced
485 E, 13.0 Ch., 33x25 Exc. Formol P Cor. 40 Al. coch. 1911 Injected (India ink)
544 E., 11.5 Ch., 30 Good Zenker-Formol P Sag. 40 Al. coch. 1911 Operative Injected (India ink)
562 E, 13.0 Ch., 28x17x17 Poor Formol P Sag. 100 Al. coch. 1912 Advanced
623 E 10.1 Good Ale. P Trans. 20 H. & Congo red 1912 Operative. Median in group
695 E, 13.5 Ch., 40x40x17 Poor Formol P Trans. 10 H. & Congo red 1913 Macerated
916 E, 11.0 Ch, 30x30x16 Good Bouin C Trans. 40 H.&E, or. G. 1915 Most-advanced third
940 E, 14.0 Ch, 28x23x21 Good Formol C Trans. 40 H.&E, or. G. 1914 Advanced
1232 E, 14.5 Ch., 35x35x30 Poor Formol P Cor. 40 Al. coch. 1915 Close to No.1267A
1267A E-a 145 Ch., 35x30x26 40 Good Formol C Sag 20 H.&E.. or. G 1915 Excellent CN.S.
1267B ?? E, 125 Good Formol p Sag. 20 H.&E. 1917 Tubal
5642 E, 11.5 Ch.. 33x30x17 Good Formol p Trans. 15 Al. coch. 1928 Right upper limb injured
5893 E.. 13.2 Good Formol C-P Trans. 20 Al. coch. 1929 Most advanced in group
6258 E. 14.0 Ch 48x35x25 Good Formol c—1= Trans. 10 H.&E. 1930 Median in group
6519 E.. 10.8* Exc Corrov acetic C-P Sag. 8 A1. coch. ? Least-advanced or middle third
6520 E., 14.2* Exc. Corros. acetic C-P Trans. 10 Al. coch. P Median in group. Ag added to slides 1-25
6521 E., 13.2* Exc. Corros. acetic C-P Trans. 8-18 Al. coch. ? Sections vary in thinness
6631 E., 13.0 Good Formol C-P Cor. 10 H.&E. 1932 Tubal. Advanced
6742 E, 11.0 Ch.,50x40x15 Good Formol C-P Trans. 12 H. & phlox. 1933 Good primary germ cells
6758 E., 12.8 Good Formol C-P Trans. 10 H. & phlox. 1933 Least—advanced third
7317 E, 10.0* Good P Cor. 10 H.&E. 1936 His embryo “Ru." Every third section
7436 E., 13.0 Good Formol C-P Cor. 30 Al. coch. 1937 Most-advanced third
8101 E., 13.0 Exc. Bouin C-P Trans. 10 H.&E. 1943 Operative
8118 E., 12.6 Exc. Bouin C-P Cor. 10 H.&E. 1943 Middle third
8253 E., 11.2 Ch.,30x20x10 Good Bouin C-P Cor. 10 Al. coch., phlox. 1944 Operative. Least advanced in group
8789 E., 11.7 Exc. Bouin C-P Sag. 10 Azan 1950
8969 E., 11.2 Exc. ? p Trans. 15 Azan 1919 Univ. Chicago No. H566
8998 E., 11.0 Exc. ? C-P Cor. 10 Azan 1952
9100 E., 12.0 Ch., 12x13x10 Exc. Formol-chrom. subl. C-P Sag. 10 Azan 1933 Univ. Chicago No. H1475
9282 E, 12.0 Ch., 16 Good Ale. p Trans. 15 Ag 1955 Mislaid


Carnegie Collection Embryos - Stage 17 
Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
353 E, 11.0 Ch., 40x35x20 Good Formalin P Coronal 10 (Stain - Haematoxylin Eosin) 1906 Very advanced
485 E, 13.0 Ch., 33x25 Exc. Formalin P Coronal 40 Al. coch. 1911 Injected (India ink)
544 E., 11.5 Ch., 30 Good Zenker-Formol P Sagittal 40 Al. coch. 1911 Operative Injected (India ink)
562 E, 13.0 Ch., 28x17x17 Poor Formalin P Sagittal 100 Al. coch. 1912 Advanced
623 E 10.1 Good Alc. P Transverse 20 H. & Congo red 1912 Operative. Median in group
695 E, 13.5 Ch., 40x40x17 Poor Formalin P Transverse 10 H. & Congo red 1913 Macerated
916 E, 11.0 Ch, 30x30x16 Good Bouin C Transverse 40 H.&E, or. G. 1915 Most-advanced third
940 E, 14.0 Ch, 28x23x21 Good Formalin C Transverse 40 H.&E, or. G. 1914 Advanced
1232 E, 14.5 Ch., 35x35x30 Poor Formalin P Coronal 40 Al. coch. 1915 Close to No.1267A
1267A E-a 145 Ch., 35x30x26 40 Good Formalin C Sagittal 20 (Stain - Haematoxylin Eosin). or. G 1915 Excellent CN.S.
1267B ?? E, 125 Good Formalin p Sagittal 20 (Stain - Haematoxylin Eosin) 1917 Tubal
5642 E, 11.5 Ch.. 33x30x17 Good Formalin p Transverse 15 Al. coch. 1928 Right upper limb injured
5893 E.. 13.2 Good Formalin C-P Transverse 20 Al. coch. 1929 Most advanced in group
6258 E. 14.0 Ch 48x35x25 Good Formalin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1930 Median in group
6519 E.. 10.8* Exc Corrov acetic C-P Sagittal 8 Al. coch. ? Least-advanced or middle third
6520 E., 14.2* Exc. Corros. acetic C-P Transverse 10 Al. coch. P Median in group. Ag added to slides 1-25
6521 E., 13.2* Exc. Corros. acetic C-P Transverse 8-18 Al. coch. ? Sections vary in thinness
6631 E., 13.0 Good Formalin C-P Coronal 10 (Stain - Haematoxylin Eosin) 1932 Tubal. Advanced
6742 E, 11.0 Ch.,50x40x15 Good Formalin C-P Transverse 12 H. & phlox. 1933 Good primary germ cells
6758 E., 12.8 Good Formalin C-P Transverse 10 H. & phlox. 1933 Least—advanced third
7317 E, 10.0* Good P Coronal 10 (Stain - Haematoxylin Eosin) 1936 His embryo “Ru." Every third section
7436 E., 13.0 Good Formalin C-P Coronal 30 Al. coch. 1937 Most-advanced third
8101 E., 13.0 Exc. Bouin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1943 Operative
8118 E., 12.6 Exc. Bouin C-P Coronal 10 (Stain - Haematoxylin Eosin) 1943 Middle third
8253 E., 11.2 Ch.,30x20x10 Good Bouin C-P Coronal 10 Al. coch., phlox. 1944 Operative. Least advanced in group
8789 E., 11.7 Exc. Bouin C-P Sagittal 10 Azan 1950
8969 E., 11.2 Exc. ? p Transverse 15 Azan 1919 Univ. Chicago No. H566
8998 E., 11.0 Exc. ? C-P Coronal 10 Azan 1952
9100 E., 12.0 Ch., 12x13x10 Exc. Formol-chrom. subl. C-P Sagittal 10 Azan 1933 Univ. Chicago No. H1475
9282 E, 12.0 Ch., 16 Good Ale. p Transverse 15 Ag 1955 Mislaid
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • Stain -
  • ? - unknown or not determined.


1987 - Stage 17 | Carnegie stage 17

Stage 18

Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Semi. ducts P.-M. duct (mm) Year Notes
109 E., 12.0* Ch.,30 Poor Alc. P Trans. 20 Al. coch. 1 0.4 1897 Tubal. Least—advanced third
144 E., 16.0* Ch, 40x30x30 Good Formol P Sag. 40 Al. coch. 3 0.85 1899 Most—advanced third
175 E., 13.0 Ch, 30x25x25 Poor Alc. P Trans. 20 Al. coch. 2 0.6 1900 Tubal. Partly macerated
296 E., 17.0 Poor Ale. P Cor. 20 Various 3 0.85 1905 Most—advanced third
317 E., 16.0 Good Formol P Cor. 20 H. &or.G. 2 0.7 1905 Middle third
351 E.,14.0* Good Formol P Cor. 250 Slightly carmine— 2 038 1904 Injected (Berlin blue)
406 E., 16.0 Ch., 40x40x40 Good Formol P Sag. 20 H.&E. 3 0.7 1907 Operative. Most—advanced third
423 E., 15.2 Good Formol—Zenker P Trans. 50 Carmine 3 0.85 1904
424 E., 172 Good Formol P Trans. 50 Carmine 3 10 1904 Double infection. Advanced
492 E, 16.8 Ch, 40 x 40 Exc. Zenker P Cor. 40 Al. coch. v 3 0.7 1911 Injected (India ink)
511 E., 160* Ch., 3?* 32x32 Good Ale. P Sag. 40 Al. coch. 3 1.1 1911 Head injured. Most advanced in group
670 E, 12.5 Poor Ale. P Sag. 50 H.&E. 3 10 1913 Tubal. Advanced
719 E, 15.0 Ch, 50x50x50 Good Formol P Trans 40 Al. coch. 2 0.6 1913 Median in group
733 E., Ch., 4Sx40x2S ISO Poor Formol P Sag. 50 Al. coch. 2 0.6 1913 Median in group
841 E. 15.0 Ch., 18 x 16x9 Good Formol P Cor.& Trans, 20 10 H.&E.. carmine 2 0.32 1914 Operative. Head cut separately
899 E, 160* Ch. 50 x 18 x IS Good Bouin P Sag. S0 Al. coch. 3 0,65 1914 Tubal Head injured
991 E. l?.0 Good Formal P Sag so R, V, Gieson 3 0.9 1914 Advanced
1909 E., 14.6 Good Formol P Cor. 20 Al. coch,or. G. 1 0.3 1917 Less advanced
2673 E.,15.5 Good Formol P Trans. 40 Al. coch. 2 0.52 1919 Median in group
4430 E., 14.0 Ch, 51 x40x21 Exc. Corros. acetic P Trans. 15 Al. coch,or. G. 3 0.9 1923 Most—advanced third
5542B E., 16.0 Ch, 37x32x25 Good Formol P Trans. 40 Al. coch. 2 0.7 1927 Other twin abnormal
5747 E, 15.2 Ch, 32x27x25 Poor Alc.—formol P Sag. 25 Al. coch. 2 0.25 1928 Least—advanced or middle third
5935A E, 13.5 Ch, 40x30x30 Good Formol P Cor. 40 Al. coch. 1 0.38 1929 Other twin stunted
6522 E, 13.2* Good Corros. acetic C—P Cor. 10 Al. coch. 3 0.8 7 Middle or most—advanced third
6524 E, 11.7* Exc. Corros. acetic C—P Trans. 10 Al. coch. 1 0.4 ? Least—advanced third
6525 E, 13.8* Exc. Corros. acetic C—P Sag 8 Al. coch. 2 0.42 ? Weak staining
6527 E, 14.4* Exc. Corros. acetic C—P Trans. 15 Al. coch. 2 0.67 ? Mechanical damage
6528 E, 13.4* Exc. Corros. acetic C—P Cor. 8 Al. coch. 1 0.33 ? Least—advanced third
6529 E, 15.6* Good Corros. acetic C—P Cor. 10 Al. coch. 2 0.4 .5 Middle third
6533 E, 12.5* Good Corros. acetic C—P Sag. 6, 8, 10 Al. coch. 2 0.45 ? Middle third
6551 E, 18.0 Poor Formol p Cor. 40 H.&E. 3 0.8 1932 Tubal
7707 E, 14.5 Ch ,37x32 Exc. Bouin C—P Trans. 10 H.&E, phlox. 2 0.54 1939 Operative. Middle third
8097 E, 15.5 Ch, 37x25x21 Good Formol C—P Trans. 10 H.&E. 1 0.19 1942 Least advanced in group
8172 E, 16.5 Exc. Bouin C—P Trans. 20 H.&E. 3 0.58 1943 Operative. Very advanced
8235 E, 14.0* Good Bouin C—P Sag. 10 H.&E., Mallory 2 0.25 1944 Tubal
8355 E, 15.0 Ch, 23 Exc. Formol C—P Cor. 10 Azan 1946 Tubal. Duplicated spinal cord caudally
8812 E, 12_9 Exc Formol C—P Trans. 10 H.&E. 1950 Rubella. Medical abortion. Midbrain punctured
8945 E, 13.9 Good Zenker p Trans. 8 Borax, carm. 1952 Univ. Chicago No. H 1254
9107 E, 17.0 Ch, 38x28x22 Good Bouin p Trans. 15 Borax, carm. 1918 Univ. Chicago No. H 516
9247 E, 15.0 Exc. Bouin C—P Sag. 8 Azan 1954 Tubal


Carnegie Collection Embryos - Stage 18 
Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Semi. ducts P.-M. duct (mm) Year Notes
109 E., 12.0* Ch.,30 Poor Alc. P Transverse 20 Al. coch. 1 0.4 1897 Tubal Least—advanced third
144 E., 16.0* Ch, 40x30x30 Good Formalin P Sagittal 40 Al. eoch. 3 0.85 1899 Most—advanced third
175 E., 13.0 Ch, 30x25x25 Poor Alc. P Transverse 20 Al. coch. 2 0.6 1900 Tubal Partly macerated
296 E., 17.0 Poor Ale. P Coronal 20 Various 3 0.85 1905 Most—advanced third
317 E., 16.0 Good Formalin P Coronal 20 (Stain - Haematoxylin Eosin) or. G. 2 0.7 1905 Middle third
351 E.,14.0* Good Formalin P Coronal 250 Slightly carmine— 2 038 1904 Injected (Berlin blue)
406 E., 16.0 Ch., 40x40x40 Good Formalin P Sagittal 20 (Stain - Haematoxylin Eosin) 3 0.7 1907 Operative. Most—advanced third
423 E., 15.2 Good Formol—Zenker P Transverse 50 Carmine 3 0.85 1904
424 E., 172 Good Formalin P Transverse 50 Carmine 3 10 1904 Double infection. Advanced
492 E, 16.8 Ch, 40 x 40 Exc. Zenker P Coronal 40 Al. coch. v 3 0.7 1911 Injected (India ink)
511 E., 160* Ch., 3?* 32x32 Good Ale. P Sagittal 40 Al. coch. 3 1.1 1911 Head injured. Most advanced in group
670 E, 12.5 Poor Ale. P Sagittal 50 (Stain - Haematoxylin Eosin) 3 10 1913 Tubal Advanced
719 E, 15.0 Ch, 50x50x50 Good Formalin P Trans 40 Al. coch. 2 0.6 1913 Median in group
733 E., Ch., 4Sx40x2S ISO Poor Formalin P Sagittal 50 Al. coch. 2 0.6 1913 Median in group
841 E. 15.0 Ch., 18 x 16x9 Good Formalin P Coronal & Trans, 20 10 (Stain - Haematoxylin Eosin), carmine 2 0.32 1914 Operative. Head cut separately
899 E, 160* Ch. 50 x 18 x IS Good Bouin P Sagittal S0 Al. coch. 3 0,65 1914 Tubal Head injured
991 E. l?.0 Good Formalin P Sag so R, V, Gieson 3 0.9 1914 Advanced
1909 E., 14.6 Good Formalin P Coronal 20 Al. coch,or. G. 1 0.3 1917 Less advanced
2673 E.,15.5 Good Formalin P Transverse 40 Al. coch. 2 0.52 1919 Median in group
4430 E., 14.0 Ch, 51 x40x21 Exc. Corros. acetic P Transverse 15 Al. coch,or. G. 3 0.9 1923 Most—advanced third
5542B E., 16.0 Ch, 37x32x25 Good Formalin P Transverse 40 Al. coch. 2 0.7 1927 Other twin abnormal
5747 E, 15.2 Ch, 32x27x25 Poor Alc.—formol P Sagittal 25 Al. coch. 2 0.25 1928 Least—advanced or middle third
5935A E, 13.5 Ch, 40x30x30 Good Formalin P Coronal 40 Al. coch. 1 0.38 1929 Other twin stunted
6522 E, 13.2* Good Corros. acetic C—P Coronal 10 Al. coch. 3 0.8 7 Middle or most—advanced third
6524 E, 11.7* Exc. Corros. acetic C—P Transverse 10 Al. coch. 1 0.4 ? Least—advanced third
6525 E, 13.8* Exc. Corros. acetic C—P Sagittal 8 Al. coch. 2 0.42 ? Weak staining
6527 E, 14.4* Exc. Corros. acetic C—P Transverse 15 Al. coch. 2 0.67 ? Mechanical damage
6528 E, 13.4* Exc. Corros. acetic C—P Coronal 8 Al. coch. 1 0.33 ? Least—advanced third
6529 E, 15.6* Good Corros. acetic C—P Coronal 10 Al. coch. 2 0.4 .5 Middle third
6533 E, 12.5* Good Corros. acetic C—P Sagittal 6, 8, 10 Al. coch. 2 0.45 ? Middle third
6551 E, 18.0 Poor Formalin p Coronal 40 (Stain - Haematoxylin Eosin) 3 0.8 1932 Tubal
7707 E, 14.5 Ch ,37x32 Exc. Bouin C—P Transverse 10 (Stain - Haematoxylin Eosin), phlox. 2 0.54 1939 Operative. Middle third
8097 E, 15.5 Ch, 37x25x21 Good Formalin C—P Transverse 10 (Stain - Haematoxylin Eosin) 1 0.19 1942 Least advanced in group
8172 E, 16.5 Exc. Bouin C—P Transverse 20 (Stain - Haematoxylin Eosin) 3 0.58 1943 Operative. Very advanced
8235 E, 14.0* Good Bouin C—P Sagittal 10 (Stain - Haematoxylin Eosin) Mallory 2 0.25 1944 Tubal
8355 E, 15.0 Ch, 23 Exc. Formalin C—P Coronal 10 Azan 1946 Tubal. Duplicated spinal cord caudally
8812 E, 12_9 Exc Formalin C—P Transverse 10 (Stain - Haematoxylin Eosin) 1950 Rubella. Medical abortion. Midbrain punctured
8945 E, 13.9 Good Zenker p Transverse 8 Borax, carm. 1952 Univ. Chicago No. H 1254
9107 E, 17.0 Ch, 38x28x22 Good Bouin p Transverse 15 Borax, carm. 1918 Univ. Chicago No. H 516
9247 E, 15.0 Exc. Bouin C—P Sagittal 8 Azan 1954 Tubal
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • Stain -
  • ? - unknown or not determined.

1987 - Stage 18 | Carnegie stage 18

Stage 19

Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Score Sex Year Notes
17 E, 18 Ch, 40x30x20 Poor Alc. P 50, 100 Al. carm. 16.5 M 1894
43 E, 16 Good Alc. P 50 Al. coch. 10 M 1 1894
293 E, 19 Poor Ale. P Sag. 50 Coch. 16.5 S 1905
390 E, 19 Good Formol? P Sag. 20, H.&E. 11.5 M 1906 Tubal. Injected

50

409 E.18 Ch, 50x40x40 Good Formol P Trans. 20 Copper, iron H. & erythrosin 14.5 M 1907
432 E..18.5 Ch , 45x35x20 Good Formol P Sag. 20 H. & Congo red 13.5 M 1910 Tubal
576 E. 17 Ch, 60x40 Good Formol P Sag. 15, 20 (Stain - Haematoxylin Eosin) 14.5 d 1912 Tubal
626 E., 21.5 Ch., 40x30x21 Good Formol P Trans. 100 Al. coch. 14_5 6 1913
6??8 E, 20 Ch, ca. 30 Poor Formol P Sag. 50 Al. coch. 12 9 1913 Head damaged
709 E, 19 Ch. 40x35x25 Poor Alc. P Car. 40 Al. coch, Lyons blue 15 49 1913
837 E. 21 Ch. 65X45x Good Formol P Sag. 40 Al. coch. 14.5 P 1914
1324 E., 18 50x30x18 Good Formol C Cor. 40 (Stain - Haematoxylin Eosin), aur, or. G 125 79 1915
1332 E., 19 Ch., 40x43x22 Poor Formol C Cor. 40 (Stain - Haematoxylin Eosin) aur, or. G. 15 M 1915
1390 E., 18 Ch, 40X38X15 Good Formol P Sag. 20 Al. coch. 10_5 M 1915 Tubal
1534 E., 13 Ch.,35x31.x25 Poor Formol P 53% 50 Al. coch. 13.5 F 1916 Protruding midbratn
2114 E., 19.3 Ch., 49x42x33 Good Formol P Trans. 40 A1. coch. 12 M 1918
4405 E., 15.5 Good Formol P Trans. 10 Coch, Mallory 13.5 <3 1923 Midbrain injured
4501 E, 18 Exc. Bouin P Trans. 15 Coch, or. G. 14.6 1924 Cystic left kidney
5609 E., 18 Exc. Formol P Cor. 25 A1. coch. 135 6
6150 E., 17 Ch., 40x39x30 Good Bouin C-P Trans. 15 (Stain - Haematoxylin Eosin) 16.5 M 1930 Tubal
6824 E., 18.5 Ch., 45x40x25 Good Formol C-P Sag. 12 (Stain - Haematoxylin Eosin) 14.5 F 1933
7900 E., 16.5 Good Bouin C-P Sag. 20 (Stain - Haematoxylin Eosin), phlox. 11.5 . . 1941 Tubal
8092 E., 16.3 Ch., 52 x 47 Exc. Bouin C-P Trans. 20 (Stain - Haematoxylin Eosin), phlox. 13 M 1942
8913 E.,? Ch, 34 Poor Formol p Trans. 10 Alan . 7 1951 Rubella. Medical abortion. Isolated head damaged
8965 E, 19.1 Ch, 42x32x19 Good Formol—Zenker C-P Trans, 10 Borax, carm, or. G. 1952 Univ. Chicago No. H 173
9097 E, 21 Exc. Fo1rno1—glucose C-P Cor. 10 Azan ? . ? 1930 Univ. Chicago No H 1380
9113 E, 185 Ch, 24 Exc. Formol C-P Trans. 10 Alan > 6 1953 Rubella. Medical abortion
9325 E, 17.0 Ch, 32x28x20 Good Formol —acetic p Trans. 15& 8-10 Azan & Ag ? - 1955 Tubal
Carnegie Collection - Stage 19 
Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Score Sex Year Notes
17 E, 18 Ch, 40x30x20 Poor Alc. P 50, 100 Al. carm. 16.5 Male 1894
43 E, 16 Good Alc. P 50 Al. coch. 10 Male 1894
293 E, 19 Poor Ale. P Sagittal 50 Coch. 16.5 S 1905
390 E, 19 Good Formol? P Sagittal 20, (Stain - Haematoxylin Eosin) 11.5 Male 1906 Tubal Injected

50

409 E.18 Ch, 50x40x40 Good Formalin P Transverse 20 Copper, iron H. & erythrosin 14.5 Male 1907
432 E..18.5 Ch , 45x35x20 Good Formalin P Sagittal 20 H. & Congo red 13.5 Male 1910 Tubal
576 E. 17 Ch, 60x40 Good Formalin P Sagittal 15, 20 (Stain - Haematoxylin Eosin) 14.5 d 1912 Tubal
626 E., 21.5 Ch., 40x30x21 Good Formalin P Transverse 100 Al. coch. 14_5 6 1913
6??8 E, 20 Ch, ca. 30 Poor Formalin P Sagittal 50 Al. coch. 12 9 1913 Head damaged
709 E, 19 Ch. 40x35x25 Poor Alc. P Coronal 40 Al. coch, Lyons blue 15 49 1913
837 E. 21 Ch. 65x45x Good Formalin P Sagittal 40 Al. coch. 14.5 P 1914
1324 E., 18 50x30x18 Good Formalin C Coronal 40 (Stain - Haematoxylin Eosin), aur, or. G 125 79 1915
1332 E., 19 Ch., 40x43x22 Poor Formalin C Coronal 40 (Stain - Haematoxylin Eosin) aur, or. G. 15 Male 1915
1390 E., 18 Ch, 40x38x15 Good Formalin P Sagittal 20 Al. coch. 10_5 Male 1915 Tubal
1534 E., 13 Ch.,35x31x25 Poor Formalin P 53% 50 Al. coch. 13.5 F 1916 Protruding midbrain
2114 E., 19.3 Ch., 49x42x33 Good Formol P Transverse 40 A1. coch. 12 M 1918
4405 E., 15.5 Good Formalin P Transverse 10 Coch, Mallory 13.5 <3 1923 Midbrain injured
4501 E, 18 Exc. Bouin P Transverse 15 Coch, or. G. 14.6 1924 Cystic left kidney
5609 E., 18 Exc. Formalin P Coronal 25 A1. coch. 13.5 Male
6150 E., 17 Ch., 40x39x30 Good Bouin C-P Transverse 15 (Stain - Haematoxylin Eosin) 16.5 Male 1930 Tubal
6824 E., 18.5 Ch., 45x40x25 Good Formalin C-P Sagittal 12 (Stain - Haematoxylin Eosin) 14.5 Female 1933
7900 E., 16.5 Good Bouin C-P Sagittal 20 (Stain - Haematoxylin Eosin), phlox. 11.5 . . 1941 Tubal
8092 E., 16.3 Ch., 52 x 47 Exc. Bouin C-P Transverse 20 (Stain - Haematoxylin Eosin), phlox. 13 Male 1942
8913 E.,? Ch, 34 Poor Formalin p Transverse 10 Alan . 7 1951 rubella. Medical abortion. Isolated head damaged
8965 E, 19.1 Ch, 42x32x19 Good Formol—Zenker C-P Transverse 10 Borax, carm, or. G. 1952 Univ. Chicago No. H 173
9097 E, 21 Exc. Formol—glucose C-P Coronal 10 Azan ? . ? 1930 Univ. Chicago No H 1380
9113 E, 185 Ch, 24 Exc. Formalin C-P Transverse 10 Alan > 6 1953 Rubella. Medical abortion
9325 E, 17.0 Ch, 32x28x20 Good Formalin —acetic p Transverse 15& 8-10 Azan & Ag ? - 1955 Tubal
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • Stain -
  • ? - unknown or not determined.

1987 - Stage 19 | Carnegie stage 19

Stage 20

Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Point Score Sex Year Notes
240 E, 20 Ch, 50x40x30 Poor Formol P Cor. 20 Iron H. 27 d 19??
256 E, 21 poor A1c p Sag. 25 Coch. 23 M 1904 Tubal. Partial anencephaly
353 E, 20 Poor A1c P Sag. 20 H. & Congo red 22,5 M 1906
431 E, 19 Ch, 30x25x25 Good Formol P Sag. 20 H. & Congo red 25,5 M 1908 Tubal
437 E, 23 Ch., 80x60x50 Poor Formol P Sag. 50 Coch 24 M 193?
453 E, 23 Ch, 60x40x30 Poor Formol P Sag. 20 H. & Congo red 23-5 ? 1910 Injected
460 E, 21 Exc. Bichlor. acetic P Trans. 40 H.&E., coch, 24.5 M 1910 injected
462 E, 20 Ch, 50x40x30 Exc. Formol P Trans. 40 Al, coch 23.5 F 1910
635B E, 22 Poor Ale P Trans 50 Al, coch 26.5 M 1913
657 E, 25 Ch, 35x20x15 Poor Formol C Sag 40 Al, coch 26.5 M 1913 Tubal
966 E, 23 Ch, 51x38x13 Exc Bichlor. acetic P Cor 40 H.&E, aur, or. G 25 M 1911 Tubal
1134B E, 23 Poor Formol p Sag 100 Al. coch., 22 - 1915
1266 E, 23.1 Poor Formol C-P Sag. 25 Al. coch., H&E aur, or G 20.5 F 191?
2393 Ch, 61.5x50x35 Poor
3527 E., 22 Ch. 32x30x10 Good Formol P Sag. 25 Al. coch., 28 ? 1921
4059 E, 21.6 Good Formol P Cor. 15 Al. coch., Mallory 29.5 $ 1922
4148 E, 21 Ch. 45x34x30 Good Formol p Con 15 A1. coch., Mallory 19 ? 1922
4361 E, 22 Ch., 52x42x23 poor Formol 9 Trans. 20 Coch. 24 8 1923
6202 E, 21 Ch., 35x35x22 Exc Bouin P Sag. 20 H.&E 20.5 8 1930 Tubal
6426 E 21.5 Good Formol C—P Trans. 20 H&E - 21 3 1931
7274 E, 18.5 Ch., 48x44x35 Exc Bouin C—P Trans. 20 H.&E., phlox. 20 M 1936
7906 E19.5 Exc Bouin C—P Cor. 20 H.&E. 22 8 1941 Left renal agenesis
8517 E., 20.8 Exc. Bouin C—P Cor. 20 H.&E. 24 8 1943
8226 E, 18.0 Exc. Bouin C—P Sag 10 Alan ? 3 1944
Carnegie Collection - Stage 20 
Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Point Score Sex Year Notes
240 E, 20 Ch, 50x40x30 Poor Formalin P Coronal 20 Iron H. 27 d 19??
256 E, 21 poor Alc p Sagittal 25 Coch. 23 M 1904 Tubal. Partial anencephaly
353 E, 20 Poor Alc P Sagittal 20 H. & Congo red 22,5 M 1906
431 E, 19 Ch, 30x25x25 Good Formalin P Sagittal 20 H. & Congo red 25,5 M 1908 Tubal
437 E, 23 Ch., 80x60x50 Poor Formalin P Sagittal 50 Coch 24 M 193?
453 E, 23 Ch, 60x40x30 Poor Formalin P Sagittal 20 H. & Congo red 23-5 ? 1910 Injected
460 E, 21 Exc. Bichlor. acetic P Transverse 40 (Stain - Haematoxylin Eosin), coch, 24.5 M 1910 injected
462 E, 20 Ch, 50x40x30 Exc. Formalin P Transverse 40 Al, coch 23.5 F 1910
635B E, 22 Poor Alc P Transverse 50 Al, coch 26.5 M 1913
657 E, 25 Ch, 35x20x15 Poor Formalin C Sagittal 40 Al, coch 26.5 M 1913 Tubal
966 E, 23 Ch, 51x38x13 Exc Bichlor. acetic P Coronal 40 (Stain - Haematoxylin Eosin), aur, or. G 25 M 1911 Tubal
1134B E, 23 Poor Formalin p Sagittal 100 Al. coch., 22 - 1915
1266 E, 23.1 Poor Formalin C-P Sagittal 25 Al. coch., (Stain - Haematoxylin Eosin) aur, or G 20.5 F 191?
2393 Ch, 61.5x50x35 Poor
3527 E., 22 Ch. 32x30x10 Good Formalin P Sagittal 25 Al. coch., 28 ? 1921
4059 E, 21.6 Good Formalin P Coronal 15 Al. coch., Mallory 29.5 $ 1922
4148 E, 21 Ch. 45x34x30 Good Formalin p Coronal 15 A1. coch., Mallory 19 ? 1922
4361 E, 22 Ch., 52x42x23 poor Formalin 9 Transverse 20 Coch. 24 8 1923
6202 E, 21 Ch., 35x35x22 Exc Bouin P Sagittal 20 (Stain - Haematoxylin Eosin) 20.5 8 1930 Tubal
6426 E 21.5 Good Formalin C—P Transverse 20 (Stain - Haematoxylin Eosin) 21 3 1931
7274 E, 18.5 Ch., 48x44x35 Exc Bouin C—P Transverse 20 (Stain - Haematoxylin Eosin), phlox. 20 M 1936
7906 E19.5 Exc Bouin C—P Coronal 20 (Stain - Haematoxylin Eosin) 22 8 1941 Left renal agenesis
8517 E., 20.8 Exc. Bouin C—P Coronal 20 (Stain - Haematoxylin Eosin) 24 8 1943
8226 E, 18.0 Exc. Bouin C—P Sagittal 10 Alan ? 3 1944
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • Stain -
  • ? - unknown or not determined.


1987 - Stage 20 | Carnegie stage 20

Stage 21

Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Point Score Sex Year Notes
22 E, 20 Ch, 35x30x30 Good Alc. P Trans. 50 Al. coch. 34.5 F 1895
57 E, 23 Ch., ca. 30 Poor Alc. P Sag. 50 Al. coch. 36 M 1896
128 E, 20 Ch., 50x43 Good Formol P Cor. 50 Al. coch. 33 F 1898
229 E, 19 Poor Alc. P Sag. 50 Al. coch. 33 F 1903
349 E, 24 Good Zenker C Cor. 250 Unstained 36 ? 1905 Double vascular injection
455 E, 24 Ch., 42x34x20 Good Alc. P Trans. 30 (Stain - Haematoxylin Eosin) 36.5 M 1910
632 E, 24 Ch., 60x50x30 Good Bichlor. acetic P Sag. 40, 100, 250 Al. coch. 33 F 1913 Injected
903C E, 23.5 Good Formol P Trans. 40 Al. coch. 38.5 F 1914
1008 E, 26,4 Good Formol P Sag. 40 Al. coch. 39 ?? 1914
1358F E, 23 Good Formol P Sag. 40 Al. coch. 37.5 F 1916
2937 E,, 24.2 Good Bouin P Trans. 50 (Stain - Haematoxylin Eosin) aur., or. G. 39 F 1920
3167 E., 24.5 Ch., 60x50x40 Poor Bichlor, acetic, formol P Trans. 20 Al. coch. 32 M 1920
4090 E, 22.2 Ch.. 66x46x30 Good Formol P Trans. 40 Al. coch. 30 F 1922
4160 E,25 Poor Formol P Sag. 25 (Stain - Haematoxylin Eosin) 39 M 1923 Tubal
4960 E.22 Ch,, 47x42x28 Good Formol P Trans. 15 Al. coch., Mallory 31.5 F 1925
5??6 E. 215 Good Formol P Sag. 20 (Stain - Haematoxylin Eosin) 34 F 1927
6531 E,22 Poor Glacial acetic, C-P Trans. 10 (Stain - Haematoxylin Eosin) 31.5 F 1931 Leitz Collection
72S4 E,225 Exc Bouin C-P Trans. 20 (Stain - Haematoxylin Eosin) 33.5 M 1936
7592 E,22-> Exc. Bouin C-P Trans. 20 (Stain - Haematoxylin Eosin) 36 F 1937
1*64 E., 24 Exc, Formol C-P Frontal 20 (Stain - Haematoxylin Eosin) 32.5 M 1941
8553 E., 22 Exc Bouin C-P Trans. 12 (Stain - Haematoxylin Eosin) 38 F 1947
9614 E,,22 5 Exc Bouin P Cor. 10 &15 Azan ? ? 1958 Rubella. Hysterectomy
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • Stain -
  • ? - unknown or not determined.


Carnegie Collection - Stage 21 
Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Point Score Sex Year Notes
22 E, 20 Ch, 35x30x30 Good Alc. P Transverse 50 Al. coch. 34.5 Female 1895
57 E, 23 Ch., ca. 30 Poor Alc. P Sagittal 50 Al. coch. 36 Male 1896
128 E, 20 Ch., 50x43 Good Formalin P Coronal 50 Al. coch. 33 Female 1898
229 E, 19 Poor Alc. P Sagittal 50 Al. coch. 33 Female 1903
349 E, 24 Good Zenker C Coronal 250 Unstained 36 ? 1905 Double vascular injection
455 E, 24 Ch., 42x34x20 Good Alc. P Transverse 30 (Stain - Haematoxylin Eosin) 36.5 Male 1910
632 E, 24 Ch., 60x50x30 Good Bichlor. acetic P Sagittal 40, 100, 250 Al. coch. 33 Female 1913 Injected
903C E, 23.5 Good Formalin P Transverse 40 Al. coch. 38.5 Female 1914
1008 E, 26,4 Good Formalin P Sagittal 40 Al. coch. 39 ?? 1914
1358F E, 23 Good Formalin P Sagittal 40 Al. coch. 37.5 Female 1916
2937 E,, 24.2 Good Bouin P Transverse 50 (Stain - Haematoxylin Eosin) aur., or. G. 39 Female 1920
3167 E., 24.5 Ch., 60x50x40 Poor Bichlor, acetic, formol P Transverse 20 Al. coch. 32 Male 1920
4090 E, 22.2 Ch.. 66x46x30 Good Formalin P Transverse 40 Al. coch. 30 Female 1922
4160 E,25 Poor Formalin P Sagittal 25 (Stain - Haematoxylin Eosin) 39 Male 1923 Tubal
4960 E.22 Ch,, 47x42x28 Good Formalin P Transverse 15 Al. coch., Mallory 31.5 Female 1925
5??6 E. 215 Good Formalin P Sagittal 20 (Stain - Haematoxylin Eosin) 34 Female 1927
6531 E,22 Poor Glacial acetic, C-P Transverse 10 (Stain - Haematoxylin Eosin) 31.5 Female 1931 Leitz Collection
7254 E,225 Exc Bouin C-P Transverse 20 (Stain - Haematoxylin Eosin) 33.5 Male 1936
7592 E,22-> Exc. Bouin C-P Transverse 20 (Stain - Haematoxylin Eosin) 36 Female 1937
7864 E., 24 Exc, Formalin C-P Frontal 20 (Stain - Haematoxylin Eosin) 32.5 Male 1941
8553 E., 22 Exc Bouin C-P Transverse 12 (Stain - Haematoxylin Eosin) 38 Female 1947
9614 E,,22 5 Exc Bouin P Coronal 10 &15 Azan ? ? 1958 Rubella. Hysterectomy
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • Stain -
  • ? - unknown or not determined.

1987 - Stage 21 | Carnegie stage 21

Stage 22

Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Point Score Sex Year Notes
392 E., 23 Ch., 45x45x25 Poor ... P Sag. 50 Al. coch. 42 M 1907 Brödel Collection. Injected
405 E., 26 Good Formol C Sag. 40 Carmine 42.5 M 1907
464 E.,26 Ch., 45x40x30 Good Formol? alc? P Sag. 100 Al. coch. 44.5 F 1910
584A E.,25 Ch., 50x42x40 Poor Formol P Sag. 50 Al. coch. 41 ? 1913
630 E., 25 Poor Formol P Trans. 100 Al. coch. 46 F 1913
840 E, 24.8 Good Formol P Trans. 50 Al. coch. 44.5 M 1914
875 E, 27 Ch., 40x28x22 Good Formol P Sag. 40 Al. coch. 45 F 1914
895 E., 26 Ch., 67x62x54 Good Formol P Trans. 25 Al. coch. 46.5 M 1914
1315 E.,25 Good Formol P Sag. 50 Al. coch. 40.5 M 1915 Spina bifida and anencephaly
1458 E., 27.5 Ch, 45x45x30 Exc. Formol C Sag. 50 H.&E. aur, or. G. 45.5 F 1916
1894 E, 24.6 Good Formol c Sag. 40,80 (Stain - Haematoxylin Eosin) aur, or. G. 41 M 1917
2206 E, 27 Ch, 50x30x18 Poor Formol p Trans. 40 H.&E. 44.5 F 1918
3681 E, 26.3 Ch, 36x36x34 Good Formol p Trans. 25 Al. coch. 44.5 F 1921
4304 E,25 Ch, 66x45x45 Good Bouin p Trans. 20 H.&E. 44.5 M 1923 Injected
4339 E, 24.5 Good Formol p Trans. 15 Al. coch, Mallory 46,5 M 1923
4476 E., 26.2 Good Bouin p Trans. 40 H.&E. 46 M 1923 Tubal
4638 E, 23.4 Exc. Bouin p Trans. 15,20 Al. coch, or. G. 41.5 ?? 1924
6701 E, 24 Poor Formol p Cor. 20 H.&E. 41 M 1933
6832 E, 25.8 Exc. Bouin C-P Cor. 20 H.&E. 42 M 1934
8394 E, 25.3 Ch, 48x50x34 Exc. Bouin C-P Trans. 20 H.&E, Masson 44.5 M 1946
8948 E, 26.7 Ch, 61x51x50 Poor Formol-Zenker p Trans. 15 Ag p . 7 1952



Carnegie Collection - Stage 22 
Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Point Score Sex Year Notes
392 E., 23 Ch., 45x45x25 Poor ... P Sagittal 50 Al. coch. 42 Female 1907 Brödel Collection. Injected
405 E., 26 Good Formalin C Sagittal 40 Carmine 42.5 Male 1907
464 E.,26 Ch., 45x40x30 Good Formol? alc? P Sag. 100 Al. coch. 44.5 Male 1910
584A E.,25 Ch., 50x42x40 Poor Formalin P Sagittal 50 Al. coch. 41 ? 1913
630 E., 25 Poor Formalin P Transverse 100 Al. coch. 46 Male 1913
840 E, 24.8 Good Formalin P Transverse 50 Al. coch. 44.5 Female 1914
875 E, 27 Ch., 40x28x22 Good Formalin P Sagittal 40 Al. coch. 45 Male 1914
895 E., 26 Ch., 67x62x54 Good Formalin P Transverse 25 Al. coch. 46.5 Female 1914
1315 E.,25 Good Formalin P Sagittal 50 Al. coch. 40.5 Female 1915 Spina bifida and anencephaly
1458 E., 27.5 Ch, 45x45x30 Exc. Formalin C Sagittal 50 (Stain - Haematoxylin Eosin) aur, or. G. 45.5 Male 1916
1894 E, 24.6 Good Formalin c Sagittal 40,80 (Stain - Haematoxylin Eosin) aur, or. G. 41 Female 1917
2206 E, 27 Ch, 50x30x18 Poor Formalin p Transverse 40 (Stain - Haematoxylin Eosin) 44.5 Male 1918
3681 E, 26.3 Ch, 36x36x34 Good Formalin p Transverse 25 Al. coch. 44.5 Male 1921
4304 E,25 Ch, 66x45x45 Good Bouin p Transverse 20 (Stain - Haematoxylin Eosin) 44.5 Female 1923 Injected
4339 E, 24.5 Good Formalin p Transverse 15 Al. coch, Mallory 46,5 Female 1923
4476 E., 26.2 Good Bouin p Transverse 40 (Stain - Haematoxylin Eosin) 46 Female 1923 Tubal
4638 E, 23.4 Exc. Bouin p Transverse 15,20 Al. coch, or. G. 41.5 Male 1924
6701 E, 24 Poor Formalin p Coronal 20 (Stain - Haematoxylin Eosin) 41 Female 1933
6832 E, 25.8 Exc. Bouin C-P Coronal 20 (Stain - Haematoxylin Eosin) 42 Female 1934
8394 E, 25.3 Ch, 48x50x34 Exc. Bouin C-P Transverse 20 (Stain - Haematoxylin Eosin), Masson 44.5 Female 1946
8948 E, 26.7 Ch, 61x51x50 Poor Formol-Zenker p Transverse 15 Ag ? ? 1952
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • Stain -
  • ? - unknown or not determined.


1987 - Stage 22 | Carnegie stage 22

Stage 23

Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Point Score Sex Year Notes
45 E,28 Ch, 40x35x20 Poor ? P Cor. trans. 50 Al. coch. 51.5 F 1895
75 E,30 Good Alc. P Sag. 50 Coch. 57 M 1897
86 E,30 Good ? ? Cor. 50 Coch. F 1897
100 E,27 Poor ? P Sag. 50 Al. coch. 57.5 ? 1897 May be an early fetus
108 E, 28 (est.) Poor Piurosulph. acid P Sag. 45 Borax carm. 52.5 F 1897
224 E30 Ch, 60x40x20 Poor Formol P Sag. 50, 100 Al. coch. 54 F 1903
417 E,32 Ch., 0x60x40 Good Formol P Trans. 100 Al. coch. 58.5 M 1907
756A E, 27 Ch. 60x45x35 Good Formol P Cor. 50 Al. coch. 56 F 1913
882 E , 28 Ch, 80x80x40 Good Formol P Trans. 40 Multiple 53 8 1913
950 E, 29 Good Formol P Trans. 50 Al. coch. 54 F 1914
1199 E.,26 Ch,, 60x40x30 Good Formol C Cor. 40 H.&E. aur , or. G. 54.5 F 1915
1535 E , 28 Ch. 50x45 x15 Poor Formol 1’ Trans. 40 H.&E. 495 F 1916
1945 E., 27.3 Ch., 83x53x22.5 Good Formol C-P Trans. 50 H.&E. aur., or. G. 48 M 1917
2561 E., 27 .5 Good Formol C-P Trans. 25 H.&E. aur., or. G. 48 .5 3 1919
4205 E., 29.5 Good Bouin P Trans. 50 A1. coch. 55.5 M 1923
4289 E., 32.2 Ch., 52x35x25 Good Formol P Trans. 15, 20 A1. coch., Mallory 59 M 1923
4525 E., 30 Good Formol P Sag. 20 H.&E. 57 1924
4570 E, 30.7 Ch., 52X50X28 Exc. Bouin P Trans. 15 H.&E., phlox. 55 1924
5154 E.,32 Good Bouin P Trans. 20 H&E. 59.5 S 1926
5422 E., 27 Good Formol P Sag. 40 H&E. 52.5 M 1927
5621A E., 27.5 Good Formol P Trans. 20 H.&E. 52.5 F 1927 Other twin has spina bifida and fused kidneys
5725 E., 23 Good Formol P Cor. 25 H.&E. aur., or. G. 50.5 M 1928
6573 E.,31.5 Good Bouin C Trans. 20 H.&E. 58.5 M 1932
7425 E., 27 Exc. Bouin C-P Cor 20 H.&E. 47 M 1937 Ag added
9226 E., 31 Exc. Formol C—P Trans. 12 Azan :> 3 1954
D.122 E, 27 Exc. . —/ ? Trans. 19 Ag . 1976 Yntema and Truex


Carnegie Collection - Stage 23 
Serial No. Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Point Score Sex Year Notes
45 E,28 Ch, 40x35x20 Poor ? P Coronal/Transverse 50 Al. coch. 51.5 Female 1895
75 E,30 Good Alc. P Sagittal 50 Coch. 57 Male 1897
86 E,30 Good ? ? Coronal 50 Coch. Male 1897 May be an early fetus
100 E,27 Poor ? P Sagittal 50 Al. coch. 57.5 ? 1897
108 E, 28 (est.) Poor Piurosulph. acid P Sagittal 45 Borax carm. 52.5 Male 1897
227 E30 Ch, 60x40x20 Poor Formalin P Sagittal 50, 100 Al. coch. 54 Female 1903
417 E,32 Ch., 0x60x40 Good Formalin P Transverse 100 Al. coch. 58.5 Female 1907
756A E, 27 Ch. 60x45x35 Good Formalin P Coronal 50 Al. coch. 56 Male 1913
882 E , 28 Ch, 80x80x40 Good Formalin P Transverse 40 Multiple 53 8 1913
950 E, 29 Good Formol P Transverse 50 Al. coch. 54 Male 1914
1199 E.,26 Ch,, 60x40x30 Good Formalin C Coronal 40 (Stain - Haematoxylin Eosin) aur , or. G. 54.5 Male 1915
1535 E , 28 Ch. 50x45 x15 Poor Formalin P Transverse 40 (Stain - Haematoxylin Eosin) 495 Female 1916
1945 E., 27.3 Ch., 83x53x22.5 Good Formalin C-P Transverse 50 (Stain - Haematoxylin Eosin) aur., or. G. 48 Male 1917
2561 E., 27 .5 Good Formalin C-P Transverse 25 (Stain - Haematoxylin Eosin) aur., or. G. 48 .5 Male 1919
4205 E., 29.5 Good Bouin P Transverse 50 A1. coch. 55.5 Female 1923
4289 E., 32.2 Ch., 52x35x25 Good Formalin P Transverse 15, 20 A1. coch., Mallory 59 Female 1923
4525 E., 30 Good Formalin P Sagittal 20 (Stain - Haematoxylin Eosin) 57 Male 1924
4570 E, 30.7 Ch., 52X50X28 Exc. Bouin P Transverse. 15 (Stain - Haematoxylin Eosin) , phlox. 55 Male 1924
5154 E.,32 Good Bouin P Transverse 20 (Stain - Haematoxylin Eosin) 59.5 Male 1926
5422 E., 27 Good Formalin P Sagittal 40 (Stain - Haematoxylin Eosin) 52.5 Female 1927
5621A E., 27.5 Good Formalin P Transverse 20 (Stain - Haematoxylin Eosin) 52.5 Male 1927 Other twin has spina bifida and fused kidneys
5725 E, 23 Good Formalin P Coronal 25 (Stain - Haematoxylin Eosin) aur., or. G. 50.5 Female 1928
6573 E,31.5 Good Bouin C Transverse 20 (Stain - Haematoxylin Eosin) 58.5 Female 1932
7425 E, 27 Exc. Bouin C-P Coronal 20 (Stain - Haematoxylin Eosin) 47 Female 1937 Ag added
9226 E, 31 Exc. Formalin C—P Transverse 12 Azan ? Female 1954
D.122 E, 27 Exc. ? ? Transverse 19 Ag ? ? 1976 Yntema and Truex
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  • Stain -
  • ? - unknown or not determined.


1987 - Stage 23 | Carnegie stage 23




1987 Stages: Introduction | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | References | Appendix 1 | Appendix 2 | Historic Papers | Embryonic Development
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