Scanning Electron Microscopy
Embryology - 15 Jun 2024 Expand to Translate |
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Introduction
The Scanning Electron Microscope (SEM) was a development of the electron microscope. Unlike a light microscope, using light, the electron microscope uses a focussed beam of electrons to image materials. The first version of this technology was the transmission electron microscope (TEM). Some example SEM images are shown on this current page.
- On this site the acronym "SEM" is used to denote a Scanning Electron Micrograph, the image produced by this form of microscopy.
A new technique called Helium Ion Microscopy (HIM) gives similar high resolution 3D images, using a scanning beam of He+ ions, from fixed tissue without the coating required for generating SEM images. Some research images examples from the rat kidney.[1]
- Links: Category:Scanning EM
Microscopy Timeline
- 1665 - Robert Hooke publishes Micrographia, a collection of biological micrographs.
- 1674 - Anton van Leeuwenhoek improved simple microscope for biological specimens.
- 1833 - Brown published a microscopic observation of orchids, describing the cell nucleus.
- 1898 - Golgi first saw and described the Golgi apparatus by staining cells with silver nitrate.
- 1931 - Ernst Ruska first transmission electron microscope, (TEM).
- 1938 - Von Ardenne first scanning electron microscope (SEM)
- 1965 - first commercial scanning electron microscopes.
- 1986 - Ernst Ruska, Gerd Binnig and Heinrich Rohrer receive the Nobel Prize in Physics for invention of the electron microscope (ER) and scanning tunneling microscope (GB and HR).
Human Embryo SEM Images
There are a series of beautiful SEM images made available by Prof Kathy Sulik of the early developing human embryo between week 3 to 5 (Carnegie stage 7 to 14) available: 7, 8, 9, 10, 11, 12, 13 and 14.
- Links: Stage 11 SEM images | Stage 12 SEM images | Stage 13 SEM images | Stage 14 SEM images | Category:Scanning EM
- Carnegie Stages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | About Stages | Timeline
Other Species SEM Images
Sea Urchin (2 cell stage) | Sea Urchin (16 cell stage) |
Chicken primordial germ cell (PGC) grown in vitro[2] | Hamster oocyte and spermatozoa |
Fly wild-type head | Fly antennapedia head |
References
- ↑ <pubmed>23505418</pubmed>| PMC3591388 | PLoS One.
- ↑ <pubmed>20886037</pubmed>| PLoS One.
Reviews
<pubmed>4571700</pubmed> <pubmed>14667297</pubmed> <pubmed>4571700</pubmed>| J Histochem Cytochem.
Articles
<pubmed>19493101</pubmed> <pubmed>16089300</pubmed> <pubmed>6798043</pubmed>
Search PubMed
Search Pubmed: Scanning Electron Microscopy
External Links
External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name. Links to any external commercial sites are provided for information purposes only and should never be considered an endorsement. UNSW Embryology is provided as an educational resource with no clinical information or commercial affiliation.
- Nobel Prize The Nobel Prize in Physics 1986 Ernst Ruska, Gerd Binnig, Heinrich Rohrer Nobel Speech
- AMMFR MyScope What is an SEM
- Dartmouth Electron Microscope Facility A variety of scanning and transmission electron microscope images (These images are in the public domain)
- University of Minnesota Bob Hafner - Scanning Electron Microscopy Primer (PDF)
- Molecular Expressions Virtual Scanning Electron Microscopy
Glossary Links
- Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link
Cite this page: Hill, M.A. (2024, June 15) Embryology Scanning Electron Microscopy. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Scanning_Electron_Microscopy
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G