User:Z5019880: Difference between revisions
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Vitrification of an oocyte entails it rapidly being cooled for preservation, where it can later be used in in-vitro fertilization (IVF). This process relies cryoproctective agents (CPAs) to increase viscosity and decrease the freezing point of the liquid within an oocyte, which in turn prevents the formation of a solid via crystallisation which could potentially kill the cell. CPAs exert osmotic stress and a level of toxicity to the oocyte, which both can be modulated by the temperature of the fluid within an oocyte. It is known that protocols surrounding the procedure of vitrification have not been consistent regarding the temperature at which the oocytes are subjected to during the procedure. In Shanshan et al., varying temperatures during the dilution step of cryopreservation by vitrification were tested to optimize such a procedure with respect to survival rates of the preserved oocytes, and the success of the proceeding IVF. | Vitrification of an oocyte entails it rapidly being cooled for preservation, where it can later be used in in-vitro fertilization (IVF). This process relies cryoproctective agents (CPAs) to increase viscosity and decrease the freezing point of the liquid within an oocyte, which in turn prevents the formation of a solid via crystallisation which could potentially kill the cell. CPAs exert osmotic stress and a level of toxicity to the oocyte, which both can be modulated by the temperature of the fluid within an oocyte. It is known that protocols surrounding the procedure of vitrification have not been consistent regarding the temperature at which the oocytes are subjected to during the procedure. In Shanshan et al., varying temperatures during the dilution step of cryopreservation by vitrification were tested to optimize such a procedure with respect to survival rates of the preserved oocytes, and the success of the proceeding IVF. | ||
In Shanshan et al. patients that were selected and sorted into groups based on whether the oocyte to be used in the IVF procedure was from a donor or | In Shanshan et al. patients that were selected and sorted into groups based on whether the oocyte to be used in the IVF procedure was from a donor or non donor (autologus). All oocytes used were subjected to the same vitrification protocols up until warming of the cryopreserved oocyte, where such as step was split into two treatments groups, those that warmed at room temperature (20-22°C) or at 37°C. At this point survival rates of the oocytes were taken, and viable oocytes were inseminated and fertilisation was evaluated shortly after. The quality of the resulting embryos were graded, which formed the basis of which embryos were implanted, where successful implantation and progression to clinical pregnancy was measured. It was found from the result of Shanshan et al. that when comparing the treatments groups, warming the oocyte after vitrification at 37°C significantly increased the chances of the oocyte surviving when thawed only for the autologous or non-donor patient group. This was thought to be due to higher temperatures increasing the permeability of CPAs, which reduced the exposure of it to the oocyte during rehydration. The differences in treatment groups appeared to have no significant effect on all the other parameters measured for both patient groups. It is such that based on the findings of Shanshan et al., vitrified oocytes should be warmed during rehydration at 37°C to increase survival rates of the oocyte for non donors. | ||
==References== | ==References== |
Revision as of 21:34, 9 August 2016
Student Information (expand to read) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Individual Assessments | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Please leave this template on top of your student page as I will add your assessment items here. Beginning your online work - Working Online in this course
Click here to email Dr Mark Hill | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lab 1 Assessment - Researching a Topic | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
In the lab I showed you how to find the PubMed reference database and search it using a topic word. Lab 1 assessment will be for you to use this to find a research reference on "fertilization" and write a brief summary of the main finding of the paper.
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Lab 2 Assessment - Uploading an Image | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OK you are now in a group
Initially the topic can be as specific or as broad as you want. Chicken embryo E-cad and P-cad gastrulation[1] References
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Lab 4 Assessment - GIT Quiz | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ANAT2341 Quiz Example | Category:Quiz | ANAT2341 Student 2015 Quiz Questions | Design 4 quiz questions based upon gastrointestinal tract. Add the quiz to your own page under Lab 4 assessment and provide a sub-sub-heading on the topic of the quiz. An example is shown below (open this page in view code or edit mode). Note that it is not just how you ask the question, but also how you explain the correct answer. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lab 5 Assessment - Course Review | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Complete the course review questionnaire and add the fact you have completed to your student page. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lab 6 Assessment - Cleft Lip and Palate | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Lab 7 Assessment - Muscular Dystrophy | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Lab 8 Assessment - Quiz | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
A brief quiz was held in the practical class on urogenital development. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lab 9 Assessment - Peer Assessment | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Lab 10 Assessment - Stem Cells | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
As part of the assessment for this course, you will give a 15 minutes journal club presentation in Lab 10. For this you will in your current student group discuss a recent (published after 2011) original research article (not a review!) on stem cell biology or technology.
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Lab 11 Assessment - Heart Development | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Read the following recent review article on heart repair and from the reference list identify a cited research article and write a brief summary of the paper's main findings. Then describe how the original research result was used in the review article.
<pubmed>26932668</pubmed>Development | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Lab Attendance
Z5019880 (talk) 14:34, 5 August 2016 (AEST)
Lab 1 Tasks
Type in group
I feel that the description of team worker best describes me with regards to trying to ensure that tasks ran as a team are ran smoothly. Particulalrly when it comes to differing ideas presented by group members, I try to ensure that I do my best to work in such away that is agreeable with all other group members. The fact that I find myself being indecisive when it comes to a choice between how an assessment should be done by group members or when appraising my own work and that of others, really makes me feel that I fit into the category of teamworker based on its description.
Lecture - Fertilization (Interesting points)
What I found most interesting in the lecture on fertilization was the removal of the extra DNA of the egg in female gametogenesis during meiosis 1 and 2. I find that the concept of division during these meiosis stages that lead to polar bodies which are asymmetrical to the egg (much smaller) to release extra DNA to ensure the egg at the end is haploid, rather than normal symmetrical division of cells is fascinating. From this interest I further researched as to how this asymmetrical division is determined by the cell, which is potentially as a result of formin - 2, a protein that can cause the placement of the meiotic spindles to be eccentric as it induces formation of actin filaments which pull chromosomes to its location (Maro & Verlhac, 2002). It is this eccentric placement of the meiotic spindles that thus leads to an asymmetrical division.
Lab 1 Assessment
<pubmed>PMC4554382</pubmed> Vitrification of an oocyte entails it rapidly being cooled for preservation, where it can later be used in in-vitro fertilization (IVF). This process relies cryoproctective agents (CPAs) to increase viscosity and decrease the freezing point of the liquid within an oocyte, which in turn prevents the formation of a solid via crystallisation which could potentially kill the cell. CPAs exert osmotic stress and a level of toxicity to the oocyte, which both can be modulated by the temperature of the fluid within an oocyte. It is known that protocols surrounding the procedure of vitrification have not been consistent regarding the temperature at which the oocytes are subjected to during the procedure. In Shanshan et al., varying temperatures during the dilution step of cryopreservation by vitrification were tested to optimize such a procedure with respect to survival rates of the preserved oocytes, and the success of the proceeding IVF.
In Shanshan et al. patients that were selected and sorted into groups based on whether the oocyte to be used in the IVF procedure was from a donor or non donor (autologus). All oocytes used were subjected to the same vitrification protocols up until warming of the cryopreserved oocyte, where such as step was split into two treatments groups, those that warmed at room temperature (20-22°C) or at 37°C. At this point survival rates of the oocytes were taken, and viable oocytes were inseminated and fertilisation was evaluated shortly after. The quality of the resulting embryos were graded, which formed the basis of which embryos were implanted, where successful implantation and progression to clinical pregnancy was measured. It was found from the result of Shanshan et al. that when comparing the treatments groups, warming the oocyte after vitrification at 37°C significantly increased the chances of the oocyte surviving when thawed only for the autologous or non-donor patient group. This was thought to be due to higher temperatures increasing the permeability of CPAs, which reduced the exposure of it to the oocyte during rehydration. The differences in treatment groups appeared to have no significant effect on all the other parameters measured for both patient groups. It is such that based on the findings of Shanshan et al., vitrified oocytes should be warmed during rehydration at 37°C to increase survival rates of the oocyte for non donors.
References
Maro, B. & Verlhac, M. (2002). Polar body formation: new rules for asymmetric divisions. Nature Cell Biology, 4(12), E281-E283. http://dx.doi.org/10.1038/ncb1202-e281
PMID 27486480
Mark Hill (talk) 12:36, 5 August 2016 (AEST) Very good. maybe a little odd in page formatting this can get messy. I will discuss in today's lab online formatting etc. |