Lecture - Week 1 and 2 Development
|Embryology - 23 Sep 2018 Expand to Translate|
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- 1 Introduction
- 2 Objectives
- 3 Lecture Resources
- 4 Fertilization
- 5 Zygote Formation
- 6 Cleavage of Zygote
- 7 Morula
- 8 Blastocyst
- 9 Week 2 - Implantation
- 10 Uterus
- 11 Conceptus
- 12 Twinning
This lecture will discuss the first two weeks of human embryogenesis and describe the cleavage stages, blastocyst formation and hatching, and the generation of the bilaminar embryo. There will also be an introduction to the uterine changes at implantation, that will be covered in detail in the placentation lecture.
Lecture slides: Beverdam Lecture Slides PDF
- Understand the events during week 1 of development (Zygote, Blastomeres, Morula, Blastocyst)
- Understand the events during week 2 of development (Trophoblast, Syncytiotrophoblast, Cytotrophoblast, Embryoblast, Implantation)
- Brief understanding of early placentation
- Brief understanding of maternal changes
|The following chapter links only work with a UNSW connection.|| The following chapter links only work with a UNSW connection.
Take the Week 1 and 2 Quiz (this is not the 2018 assessment quiz that will be held in the practical class).
Fertilization - Spermatozoa
- Sperm Binding - zona pellucida protein ZP3 acts as receptor for sperm
- Acrosome Reaction - exyocytosis of acrosome contents (Calcium mediated) MBoC - Figure 20-31. The acrosome reaction that occurs when a mammalian sperm fertilizes an egg
- enzymes to digest the zona pellucida, exposes sperm surface proteins to bind ZP2
- Membrane Fusion - between spermatozoa and oocyte, allows spermatozoa nuclei passage into oocyte cytoplasm
- Membrane Depolarization - caused by sperm membrane fusion, primary block to polyspermy
- Cortical Reaction - IP3 pathway elevates intracellular Calcium, exocytosis of cortical granules MBoC - Figure 20-32. How the cortical reaction in a mouse egg is thought to prevent additional sperm from entering the egg
- enzyme alters ZP3 so it will no longer bind sperm plasma membrane
- Meiosis 2 - completion of 2nd meiotic division
- forms second polar body (a third polar body may be formed by meiotic division of the first polar body)
- zygote (Carnegie stage 1) is the first diploid cell formed following fertilisation.
- male and female pronuclei, 2 nuclei approach each other and nuclear membranes break down.
- DNA replicates, first mitotic division
- sperm contributes centriole which organizes mitotic spindle
|Pronuclear Fusion and Parental Genomes Movies|
- Conceptus - the term refers to all material derived from this fertilised zygote, includes both the embryo and the non-embryonic tissues (placenta, fetal membranes).
Cleavage of Zygote
|Zygote Division Movie|
Human Zygote to Blastocyst Development (day 1 to 6)
- Links: Carnegie stage 2 |
|Human Embryo (day 2)||Human Embryo (day 3)|
- about day 4 is a solid ball of 16-20 cells with peripheral cells flattened against zona pellucida
- compaction occurs forming a cavity and leading to the next blastocyst stage
- about day 5 have 2 identifiable cell types and a fluid-filled cavity (blastoceol)
- outer cell layer - trophoblast, peripheral flattened cells, forms the placenta and placental membranes
- inner cell mass - embryoblast, mass of rounder cells located on one wall of the blastocoel, forms entire embryo
|Human Blastocyst Movies|
Blastula Cell Communication
Two forms of cellular junctions
- tight junctions, close to outer surface create a seal, isolates interior of embryo from external medium
- gap junctions, allow electrically couple cells of epithelium surrounding a fluid-filled cavity
|Tight junctions||Gap junctions|
|Human Blastocyst Hatching Movie|
Blastocyst Hatching - zona pellucida lost, ZP has sperm entry site, and entire ZP broken down by uterine secretions and possibly blastula secretions. Uterine Glands - secretions required for blastocyst motility and nutrition
Week 2 - Implantation
The second week of human development is concerned with the process of implantation and the differentiation of the blastocyst into early embryonic and placental forming structures.
Normal Implantation Sites - in uterine wall superior, posterior, lateral
|Ectopic Ultrasound Movie|
Abnormal implantation sites or Ectopic Pregnancy occurs if implantation is in uterine tube or outside the uterus.
- sites - external surface of uterus, ovary, bowel, gastrointestinal tract, mesentry, peritoneal wall
- If not spontaneous then, embryo has to be removed surgically
Tubal pregnancy - 94% of ectopic pregnancies
- if uterine epithelium is damaged (scarring, pelvic inflammatory disease)
- if zona pellucida is lost too early, allows premature tubal implantation
- embryo may develop through early stages, can erode through the uterine horn and reattach within the peritoneal cavity
- Compact - implantation occurs in this layer, dense stromal cells, uterine gland necks, capillaries of spiral arteries
- Spongy - swollen stromal cells, uterine gland bodies, spiral arteries
- Basal - not lost during menstruation or childbirth, own blood supply
- transformation of endometrial stromal cells
- occurs initially at site of implantation and includes both cellular and matrix changes
- reaction spreads throughout entire uterus, not at cervix
- deposition of fibrinoid and glycogen and epithelial plaque formation (at anchoring villi)
- presence of decidual cells are indicative of pregnancy
Other Uterine Changes
- Cervix - at mouth of uterus, secretes mucus (CMP), forms a plug/barrier, mechanical and antibacterial
- Vascular - increased number of blood vessels
The endometrium becomes the decidua and forms 3 distinct anatomical regions (at approx 3 weeks)
- Decidua Basalis at implantation site
- Decidua Capsularis enclosing the conceptus
- Decidua Parietalis the remainder of uterus
- Decidua Capsularis and Parietalis fuse eventually fuse and uterine cavity is lost by 12 weeks
Endometriosis endometrial tissue located in other regions of the uterus or other tissues. This misplaced tissue develops into growths or lesions which respond to the menstrual cycle hormonal changes in the same way that the tissue of the uterine lining does; each month the tissue builds up, breaks down, and sheds.
Two trophoblast layers Cytotrophoblast and Syncitiotrophoblast.
Cytotrophoblasts - form a continuous cellular layer that covers the developing placental villi.
- secrete proteolytic enzymes, enzymes break down extracellular matrix around cells
- Allow passage of blastocyst into endometrial wall, totally surround the blastocyst
- generate spaces that fill with maternal blood- lacunae
- secrete Human Chorionic Gonadotropin (hCG), hormone, maintains decidua and Corpus Luteum, basis of pregnancy diagnostic test, present in urine is diagnostic of pregnancy
- levels peak at 8 to 10 weeks of pregnancy, then decline and are lower for rest of pregnancy
- 1-2 months: 5,000-200,000 mIU/ml; Non-pregnant females: <5.0 mIU/ml; Postmenopausal females: <9.5 mIU/ml)
- Later in development placenta will secrete hCG
|Human ovary corpus luteum||Trophoblast hCG||Pregnancy Test Kit|
Twinning can be due to two separate fertilization events (dizygotic twins) or as an abnormality of a single fertilization (monozygotic twins) event during the early weeks of development.
- In dizygotic twinning the genetic material is different and implantation and placentation is also different.
- In monozygotic twinning the genetic material is initially identical and degree of twinning will depend upon the timing (early to late) from separate fetal membranes and placenta to conjoined twins.
- morula stage (diamniotic dichorionic), early blastocyst (diamniotic monochorionic), late blastocyst to bilaminar (monoamniotic monochorionic), bilaminar to trilaminar embryo (conjoined)
- Monozygotic twins are a unique research resource for comparing environmental effects on development and health.
- Congenital abnormality statistics for twins is generally increased in various conditions.
Monoygotic twins (identical) produced from a single fertilization event (one fertilised egg and a single spermatazoa, form a single zygote), these twins therefore share the same genetic makeup. Occurs in approximately 3-5 per 1000 pregnancies, more commonly with aged mothers. The later the twinning event, the less common are initially separate placental membranes and finally resulting in conjoined twins.
|Week||Week 1||Week 2|
|Event||Ovulation||fertilization||First cell division||Morula||Early blastocyst|| Late blastocyst
|Implantation starts||X inactivation|
Table based upon recent Twinning Review.
- Judith G Hall Twinning. Lancet: 2003, 362(9385);735-43 PubMed 12957099
Now watch the Week 1 overview.
|Week 1 Movie|