|Embryology - 19 Jun 2018 Expand to Translate|
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- 1 Introduction
- 2 Some Recent Findings
- 3 Movies
- 4 Human Blastocyst Formation
- 5 Labeled Blastocyst
- 6 Blastocyst Hatching
- 7 Model Human Blastocyst Development
- 8 Mouse Blastocyst Gene Expression
- 9 Inner Cell Mass
- 10 Trophectoderm
- 11 Blastocoel Formation
- 12 Blastocyst Metabolism
- 13 Blastula Cell Communication
- 14 Blastocyst Hatching
- 15 Molecular Factors
- 16 Blastocyst in Other Species
- 17 References
- 18 Glossary Links
(Greek, blastos = sprout + cystos = cavity) or blastula, the term used to describe the hollow cellular mass that forms in early development.
The blastocyst consists of cells forming an outer trophectoderm (TE, trophoblast) layer, an inner cell mass (ICM, embryo blast) and a blastocoel (fluid-filled cavity). The blastocyst inner cell mass is the source of true embryonic stem cells capable of forming all cell types within the embryo.
In humans, blastocyst stage of development occurs during the first and second week following fertilization (GA week 3 and 4) and is described initially as Carnegie stage 3. This stage is followed by blastocyst hatching and implantation.
One of the first key developmental patterning decisions in the morula to blastocyst development is TE or ICM cell fate. In the mouse model, Hippo(TEAD4) and Notch(Cdx2) together appear regulate this early fate decision.
|Blastocyst Links: blastocyst | morula | fertilization | Week 1 | trophoblast | implantation | Human Day 3-6 Movie | Mouse Model Movie | Category:Blastocyst|
|Molecular: Hippo | Notch|
Some Recent Findings
|More recent papers|
This table shows an automated computer PubMed search using the listed sub-heading term.
References listed on the rest of the content page and the associated discussion page (listed under the publication year sub-headings) do include some editorial selection based upon both relevance and availability.
Emma Adolfsson, Anna Nowosad Andershed Morphology vs morphokinetics: a retrospective comparison of inter-observer and intra-observer agreement between embryologists on blastocysts with known implantation outcome. JBRA Assist Reprod: 2018; PubMed 29912521
Danilo Cimadomo, Alberto Vaiarelli, Silvia Colamaria, Elisabetta Trabucco, Carlo Alviggi, Roberta Venturella, Erminia Alviggi, Ramona Carmelo, Laura Rienzi, Filippo Maria Ubaldi Luteal phase anovulatory follicles result in the production of competent oocytes: intra-patient paired case-control study comparing follicular versus luteal phase stimulations in the same ovarian cycle. Hum. Reprod.: 2018; PubMed 29912374
Z Jiang, J Lin, H Dong, X Zheng, S L Marjani, J Duan, Z Ouyang, J Chen, X C Tian DNA methylomes of bovine gametes and in vivo produced preimplantation embryos. Biol. Reprod.: 2018; PubMed 29912291
Leona Matsumoto, Yasushi Hirota, Tomoko Saito-Fujita, Norihiko Takeda, Tomoki Tanaka, Takehiro Hiraoka, Shun Akaeda, Hidetoshi Fujita, Ryoko Shimizu-Hirota, Shota Igaue, Mitsunori Matsuo, Hirofumi Haraguchi, Mayuko Saito-Kanatani, Tomoyuki Fujii, Yutaka Osuga HIF2α in the uterine stroma permits embryo invasion and luminal epithelium detachment. J. Clin. Invest.: 2018; PubMed 29911998
Kyung-Tae Shin, Jing Guo, Ying-Jie Niu, Xiang-Shun Cui The toxic effect of aflatoxin B1 on early porcine embryonic development. Theriogenology: 2018, 118;157-163 PubMed 29909260
- Compaction - morula blastomeres packing tightly (microfilament cytoskeleton)
- Cleavage planes - spindle direction of dividing cells (mitosis).
- Polarisation - blastomeres apico-basal (Hippo pathway, Yes-associated protein (Yap)
- Cavitation - blastocoel formation with cycles of cavity expansion and collapse.
- epiblast cells - contact the polar trophectoderm
- primitive endoderm - facing the cavity
Human Blastocyst Formation
|Blastocyst hatching from zona pellucida (mouse)||Blastocyst hatching from zona pellucida (human)|
Model Human Blastocyst Development
The following figure is from a recent study using video and genetic analysis of in vitro human development during week 1 following fertilization.
- EGA - embryonic genome activation
- ESSP - embryonic stage–specific pattern, four unique embryonic stage–specific patterns (1-4)
- Links: Figure with legend
Mouse Blastocyst Gene Expression
General gene expression patterns are indicated from genomic profiling.
- red - loss of maternal mRNAs
- green - activation of embryonic genome (EGA)
- purple - maternal gene activation (MGA)
- orange - continuous expression
Inner Cell Mass
The inner cell mass forms an inner layer of larger cells is also called the "embryoblast" is a cluster of cells located and attached on one wall of the outer trophoblast layer. In week 2 this mass will differentiate into two distinct layers the epiblast and hypoblast.
The epiblast layer will form the entire embryo and undergoes gastrulation in week three to form the 3 germ layers. It also forms an epithelial layer lining the amniotic cavity.
The trophectoderm (TE) outer layer of smaller cells is also called the "trophoblast" epithelium. A key early function is for the transport of sodium (Na+) and chloride (Cl-) ions through this layer into the blastocoel. Later in week 2 this layer will differentiate into two distinct trophoblast layers the syncytiotrophoblast and cytotrophoblast cells and are key to implantation and early placentation.
Differentiation of the early layer has been shown to be regulated by the transcription factors Tead4 and then Caudal-related homeobox 2 (Cdx2).
- trophectoderm transports of Na+ and Cl- ions through this layer into the blastocoel
- generates an osmotic gradient driving fluid across this epithelium
- distinct apical and basolateral membrane domains specific for transport
- facilitates transepithelial Na+ and fluid transport for blastocoel formation
- transport is driven by Na, K-adenosine triphosphatase (ATPase) in basolateral membranes of the trophectoderm 
At the blastocyst stage, mammalian development metabolism switches on anaerobic glycolysis metabolism to satisfy metabolic demands of growing blastocyst and formation of the blastocoel. This is thought to be driven by the integral membrane protein family of facilitative glucose transporters (GLUT or SLC2A).
- aerobic - oxidation of lactate and pyruvate via the citric acid cycle (Krebs cycle) and oxidative phosphorylation
- glycolysis- converts glucose into pyruvate
- GLUT - GLUcose Transporter (divided into 3 classes I-III)
- SLC2 - Solute Carrier Family 2
Glucose Transporter Expression
- GLUT1 - from zygote to blastocyst. (all mammalian tissues, basal glucose uptake)
- GLUT2 and GLUT3 - from late eight cell stage to blastocyst. (GLUT2, liver and pancreatic beta cells; GLUT3, all mammalian tissues, basal glucose uptake)
- GLUT4 - not expressed. (muscle and adipose tissue)
- GLUT8 - up-regulated at blastocyst stage. (central nervous system and heart)
- (Data mainly from mouse development, adult tissue expression shown in brackets)
A mouse study, has shown GLUT8 is up-regulated following insulin stimulation, though a more recent GLUT8 knockout mouse shows normal early embryonic development in the absence of this transporter.
Blastula Cell Communication
Two types of cell junctions have been identified located at different regions in the developing blastocyst.
| Tight junctions
Located close to outer surface create a seal, isolates interior of embryo from external medium.
| Gap junctions
Allow electrically coupling of the cells of epithelium surrounding the fluid-filled cavity.
Blastocyst Hatching - zona pellucida lost, ZP has sperm entry site, and entire ZP broken down by uterine secretions and possibly blastula secretions.
Uterine Glands - secretions required for blastocyst motility and nutrition
|At about day 5 the human blastocyst "hatches" out of the protective zona pellucida. This hatching allows increased growth, access to uterine nutrient secretions and blastocyst adhesion to the uterine lining. Associated with this hatching process are a series of physical contractions.
||<mediaplayer width='500' height='450' image="http://php.med.unsw.edu.au/embryology/images/a/af/Human_blastocyst_day_5-6.jpg">File:Human_blastocyst_day_5-6.mp4</mediaplayer>
Human blastocyst contractions (day 5-6)
- TEA DNA- binding domain, these factors bind to the consensus TEA/ATTS cognate binding site
- TEF-3 - renamed Tead1 and Tead4
- Tead3 - is expressed in the placental syncytiotrophoblasts
- E-cadherin - Calcium ion-dependent cell adhesion molecule, a cell membrane adhesive protein required for morula compaction
- epithin - A type II transmembrane serine protease, identified in mouse for compaction of the morula during preimplantation embryonic development. Expressed from 8-cell stage at blastomere contacts and co-localises in the morula with E-cadherin.
- Na, K-adenosine triphosphatase - A sodium potassium pump that generates an osmotic gradient for fluid flow into the blastocoel
- Zonula occludens-1 - (ZO-1) Tight junction protein involved in morula to blastocyst transformation in the mouse 
Blastocyst in Other Species
Early mouse development model
- Links: Mouse Development
- Links: Bovine Development
- Zhang P, Zucchelli M, Bruce S, Hambiliki F, Stavreus-Evers A, Levkov L, Skottman H, Kerkelä E, Kere J & Hovatta O. (2009). Transcriptome profiling of human pre-implantation development. PLoS ONE , 4, e7844. PMID: 19924284 DOI.
- Sasaki H. (2015). Position- and polarity-dependent Hippo signaling regulates cell fates in preimplantation mouse embryos. Semin. Cell Dev. Biol. , 47-48, 80-7. PMID: 25986053 DOI.
- Rayon T, Menchero S, Nieto A, Xenopoulos P, Crespo M, Cockburn K, Cañon S, Sasaki H, Hadjantonakis AK, de la Pompa JL, Rossant J & Manzanares M. (2014). Notch and hippo converge on Cdx2 to specify the trophectoderm lineage in the mouse blastocyst. Dev. Cell , 30, 410-22. PMID: 25127056 DOI.
- Nissen SB, Perera M, Gonzalez JM, Morgani SM, Jensen MH, Sneppen K, Brickman JM & Trusina A. (2017). Four simple rules that are sufficient to generate the mammalian blastocyst. PLoS Biol. , 15, e2000737. PMID: 28700688 DOI.
- Maître JL, Turlier H, Illukkumbura R, Eismann B, Niwayama R, Nédélec F & Hiiragi T. (2016). Asymmetric division of contractile domains couples cell positioning and fate specification. Nature , 536, 344-348. PMID: 27487217 DOI.
- Le Bin GC, Muñoz-Descalzo S, Kurowski A, Leitch H, Lou X, Mansfield W, Etienne-Dumeau C, Grabole N, Mulas C, Niwa H, Hadjantonakis AK & Nichols J. (2014). Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst. Development , 141, 1001-10. PMID: 24504341 DOI.
- Wong CC, Loewke KE, Bossert NL, Behr B, De Jonge CJ, Baer TM & Reijo Pera RA. (2010). Non-invasive imaging of human embryos before embryonic genome activation predicts development to the blastocyst stage. Nat. Biotechnol. , 28, 1115-21. PMID: 20890283 DOI.
- Parks JC, McCallie BR, Janesch AM, Schoolcraft WB & Katz-Jaffe MG. (2011). Blastocyst gene expression correlates with implantation potential. Fertil. Steril. , 95, 1367-72. PMID: 20864103 DOI.
- Yamanaka Y, Lanner F & Rossant J. (2010). FGF signal-dependent segregation of primitive endoderm and epiblast in the mouse blastocyst. Development , 137, 715-24. PMID: 20147376 DOI.
- Fong CY & Bongso A. (1999). Comparison of human blastulation rates and total cell number in sequential culture media with and without co-culture. Hum. Reprod. , 14, 774-81. PMID: 10221713
- Tanaka N, Takeuchi T, Neri QV, Sills ES & Palermo GD. (2006). Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model. J Transl Med , 4, 20. PMID: 16681851 DOI.
- Bell CE, Calder MD & Watson AJ. (2008). Genomic RNA profiling and the programme controlling preimplantation mammalian development. Mol. Hum. Reprod. , 14, 691-701. PMID: 19043080 DOI.
- Nishioka N, Yamamoto S, Kiyonari H, Sato H, Sawada A, Ota M, Nakao K & Sasaki H. (2008). Tead4 is required for specification of trophectoderm in pre-implantation mouse embryos. Mech. Dev. , 125, 270-83. PMID: 18083014 DOI.
- Kidder GM & Watson AJ. (2005). Roles of Na,K-ATPase in early development and trophectoderm differentiation. Semin. Nephrol. , 25, 352-5. PMID: 16139691 DOI.
- Carayannopoulos MO, Chi MM, Cui Y, Pingsterhaus JM, McKnight RA, Mueckler M, Devaskar SU & Moley KH. (2000). GLUT8 is a glucose transporter responsible for insulin-stimulated glucose uptake in the blastocyst. Proc. Natl. Acad. Sci. U.S.A. , 97, 7313-8. PMID: 10860996
- Membrez M, Hummler E, Beermann F, Haefliger JA, Savioz R, Pedrazzini T & Thorens B. (2006). GLUT8 is dispensable for embryonic development but influences hippocampal neurogenesis and heart function. Mol. Cell. Biol. , 26, 4268-76. PMID: 16705176 DOI.
- Sueo Niimura Time-lapse videomicrographic analyses of contractions in mouse blastocysts. J. Reprod. Dev.: 2003, 49(6);413-23 PubMed 14967891
- Yamazaki K & Kato Y. (1989). Sites of zona pellucida shedding by mouse embryo other than muran trophectoderm. J. Exp. Zool. , 249, 347-9. PMID: 2708952 DOI.
- Jacquemin P, Hwang JJ, Martial JA, Dollé P & Davidson I. (1996). A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain. J. Biol. Chem. , 271, 21775-85. PMID: 8702974
- Khang I, Sonn S, Park JH, Rhee K, Park D & Kim K. (2005). Expression of epithin in mouse preimplantation development: its functional role in compaction. Dev. Biol. , 281, 134-44. PMID: 15848395 DOI.
- Wang H, Ding T, Brown N, Yamamoto Y, Prince LS, Reese J & Paria BC. (2008). Zonula occludens-1 (ZO-1) is involved in morula to blastocyst transformation in the mouse. Dev. Biol. , 318, 112-25. PMID: 18423437 DOI.
- Keramari M, Razavi J, Ingman KA, Patsch C, Edenhofer F, Ward CM & Kimber SJ. (2010). Sox2 is essential for formation of trophectoderm in the preimplantation embryo. PLoS ONE , 5, e13952. PMID: 21103067 DOI.
- Krupinski P, Chickarmane V & Peterson C. (2011). Simulating the mammalian blastocyst--molecular and mechanical interactions pattern the embryo. PLoS Comput. Biol. , 7, e1001128. PMID: 21573197 DOI.
Rossant J. (2007). Stem cells and lineage development in the mammalian blastocyst. Reprod. Fertil. Dev. , 19, 111-8. PMID: 17389140
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Sheth B, Nowak RL, Anderson R, Kwong WY, Papenbrock T & Fleming TP. (2008). Tight junction protein ZO-2 expression and relative function of ZO-1 and ZO-2 during mouse blastocyst formation. Exp. Cell Res. , 314, 3356-68. PMID: 18817772 DOI.
Nishioka N, Yamamoto S, Kiyonari H, Sato H, Sawada A, Ota M, Nakao K & Sasaki H. (2008). Tead4 is required for specification of trophectoderm in pre-implantation mouse embryos. Mech. Dev. , 125, 270-83. PMID: 18083014 DOI.
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Cite this page: Hill, M.A. (2018, June 19) Embryology Blastocyst Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Blastocyst_Development
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