Zebrafish Development: Difference between revisions
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* '''Zebrafish znfl1s regulate left-right asymmetry patterning through controlling the expression of fgfr1a'''{{#pmid:30317609|PMID30317609}} "Proper left-right (LR) axis establishment is critical for organogenesis in vertebrates. Previously, we reported that zinc finger transcription factors zinc finger transcription factor 1 (znfl1s) are expressed in the tailbud and axial mesoderm in zebrafish. However, a role of znfl1s in LR axis development has not been demonstrated. Here, we discovered that the knockdown of znfl1s using morpholino (MO) in whole embryos or dorsal forerunner cells (DFCs) interrupted LR asymmetry and normal development of the heart, liver, and pancreas. Whole-embryo knockdown of znfl1s by MO or clustered regularly interspaced short palindromic repeat (CRISPR) interference (CRISPRi) resulted in the absent expression of nodal gene spaw and Nodal signaling-related genes lft1, lft2, and pitx2c in the left lateral plate mesoderm (LPM), and Spaw, Lft1, Lft2, and Pitx2c play important roles in LR axis development in zebrafish. However, specific knockdown of znfl1s in DFCs resulted in random expression of spaw, lft1, lft2, and pitx2c. Knockdown of znfl1s led to abnormal cilia formation by the downregulation of fgfr1a and foxj1a expression. The expression of spaw, lft1, lft2, and pitx2c was partially rescued by the overexpression of fgfr1a mRNA in znfl1s morphants. Taken together, our results suggest that znfl1s regulate laterality development in zebrafish embryos through controlling the expression of fgfr1a." | * '''Zebrafish znfl1s regulate left-right asymmetry patterning through controlling the expression of fgfr1a'''{{#pmid:30317609|PMID30317609}} "Proper left-right (LR) axis establishment is critical for organogenesis in vertebrates. Previously, we reported that zinc finger transcription factors zinc finger transcription factor 1 (znfl1s) are expressed in the tailbud and axial mesoderm in zebrafish. However, a role of znfl1s in LR axis development has not been demonstrated. Here, we discovered that the knockdown of znfl1s using morpholino (MO) in whole embryos or dorsal forerunner cells (DFCs) interrupted LR asymmetry and normal development of the heart, liver, and pancreas. Whole-embryo knockdown of znfl1s by MO or clustered regularly interspaced short palindromic repeat (CRISPR) interference (CRISPRi) resulted in the absent expression of nodal gene spaw and Nodal signaling-related genes lft1, lft2, and pitx2c in the left lateral plate mesoderm (LPM), and Spaw, Lft1, Lft2, and Pitx2c play important roles in LR axis development in zebrafish. However, specific knockdown of znfl1s in DFCs resulted in random expression of spaw, lft1, lft2, and pitx2c. Knockdown of znfl1s led to abnormal cilia formation by the downregulation of fgfr1a and foxj1a expression. The expression of spaw, lft1, lft2, and pitx2c was partially rescued by the overexpression of fgfr1a mRNA in znfl1s morphants. Taken together, our results suggest that znfl1s regulate laterality development in zebrafish embryos through controlling the expression of fgfr1a." | ||
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* '''Zebrafish Pronephros Development'''{{#pmid:28409341|PMID28409341}} "The pronephros is the first kidney type to form in vertebrate embryos. The first step of pronephrogenesis in the zebrafish is the formation of the intermediate mesoderm during gastrulation, which occurs in response to secreted morphogens such as BMPs and Nodals. Patterning of the intermediate mesoderm into proximal and distal cell fates is induced by retinoic acid signaling with downstream transcription factors including wt1a, pax2a, pax8, hnf1b, sim1a, mecom, and irx3b. In the anterior intermediate mesoderm, progenitors of the glomerular blood filter migrate and fuse at the midline and recruit a blood supply. More posteriorly localized tubule progenitors undergo epithelialization and fuse with the cloaca. The Notch signaling pathway regulates the formation of multi-ciliated cells in the tubules and these cells help propel the filtrate to the cloaca. The lumenal sheer stress caused by flow down the tubule activates anterior collective migration of the proximal tubules and induces stretching and proliferation of the more distal segments. Ultimately these processes create a simple two-nephron kidney that is capable of reabsorbing and secreting solutes and expelling excess water-processes that are critical to the homeostasis of the body fluids. The zebrafish pronephric kidney provides a simple, yet powerful, model system to better understand the conserved molecular and cellular progresses that drive nephron formation, structure, and function." {{Renal}} | |||
* '''A crystal-clear zebrafish for in vivo imaging'''{{#pmid:27381182|PMID27381182}} "Here we present crystal, an optically clear zebrafish mutant obtained by combining different viable mutations affecting skin pigmentation. Compared to the previously described combinatorial mutant casper, the crystal mutant lacks pigmentation also in the retinal pigment epithelium, therefore enabling optical access to the eyes. Unlike PTU-treated animals, crystal larvae are able to perform visually guided behaviours, such as the optomotor response, as efficiently as wild type larvae. To validate the in vivo application of crystal larvae, we performed whole-brain light-sheet imaging and two-photon calcium imaging of neural activity in the retina." | * '''A crystal-clear zebrafish for in vivo imaging'''{{#pmid:27381182|PMID27381182}} "Here we present crystal, an optically clear zebrafish mutant obtained by combining different viable mutations affecting skin pigmentation. Compared to the previously described combinatorial mutant casper, the crystal mutant lacks pigmentation also in the retinal pigment epithelium, therefore enabling optical access to the eyes. Unlike PTU-treated animals, crystal larvae are able to perform visually guided behaviours, such as the optomotor response, as efficiently as wild type larvae. To validate the in vivo application of crystal larvae, we performed whole-brain light-sheet imaging and two-photon calcium imaging of neural activity in the retina." |
Revision as of 10:17, 30 April 2020
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Introduction
Zebrafish or zebra danio (danio rerio) are seen as one of the latest "models" for vertebrate embryological development studies. These embryos have the great advantage that they develop as "see through" embryos, that is, all internal development can be clearly observed from the outside in the living embryo. Much of the early modern work using this embryo model began with the papers of Kimmel.[1][2]
Several large laboratories in the US are now developing large breeding programs to carry out "knockouts" and to find spontaneous mutants of interest.
Fish Links: Zebrafish Development | Medaka Development | Salmon Development | Movie - Zebrafish Heart | Student Group Project - Zebrafish | Recent References | Category:Zebrafish | Category:Medaka |
Some Recent Findings
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More recent papers |
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This table allows an automated computer search of the external PubMed database using the listed "Search term" text link.
More? References | Discussion Page | Journal Searches | 2019 References | 2020 References Search term: Zebrafish Embryology | Zebrafish Development |
Older papers |
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These papers originally appeared in the Some Recent Findings table, but as that list grew in length have now been shuffled down to this collapsible table.
See also the Discussion Page for other references listed by year and References on this current page.
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Movies
Movie of an immobilized zebrafish embryo development from the 1-cell stage to 85 hours post fertilisation (hpf).[13]
<html5media height="300" width="948">File:zebrafish movie01.mp4</html5media> | |||
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Zebrafish Stages
Pharyngula Period
- Transition from Prim 5 to Long-pec
- The body axis begins to straighten and the head straightens out and lifts dorsally
- Notochord is well developed
- Formation of the Dorsal and Ventral Stripe
- Nervous system is hollow and expanding anteriorly
- The brain has developed into 5 distinct lobes
- Seven pharyngeal arch's develop rapidly during this stage
- Pectoral fins begin to develop
- The Circulatory system develops and the heart beats for the first time
- Blood begins to circulate through a closed circuit of channels
- Tactile sensitivity appears and uncoordinated movements occur
Skull
Zebrafish Skull Neural Crest Contribution [14]
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Neural
Sensory
Lateral line is a zebrafish sensory system, used to detect changes in water flow, composed of clusters of mechanosensory hair cells called neuromasts.
Molecular
Fibroblast Growth Factor
- Fgf8 and Fgf3 - regulating the segmentation of the pharyngeal endoderm into pouches.[15]
- Fgf24 and Fgf8 - promotes posterior mesodermal development.[16]
- Sox9 - required for cartilage morphogenesis.[17]
References
- ↑ Kimmel CB, Sessions SK & Kimmel RJ. (1981). Morphogenesis and synaptogenesis of the zebrafish Mauthner neuron. J. Comp. Neurol. , 198, 101-20. PMID: 7229136 DOI.
- ↑ Kimmel CB, Sepich DS & Trevarrow B. (1988). Development of segmentation in zebrafish. Development , 104 Suppl, 197-207. PMID: 3077108
- ↑ 3.0 3.1 Muto A, Calof AL, Lander AD & Schilling TF. (2011). Multifactorial origins of heart and gut defects in nipbl-deficient zebrafish, a model of Cornelia de Lange Syndrome. PLoS Biol. , 9, e1001181. PMID: 22039349 DOI.
- ↑ Nagpal J, Herget U, Choi MK & Ryu S. (2019). Anatomy, development, and plasticity of the neurosecretory hypothalamus in zebrafish. Cell Tissue Res. , 375, 5-22. PMID: 30109407 DOI.
- ↑ Pennisi E. (2018). Development cell by cell. Science , 362, 1344-1345. PMID: 30573610 DOI.
- ↑ Li J, Gao F, Zhao Y, He L, Huang Y, Yang X, Zhou Y, Yu L, Zhao Q & Dong X. (2019). Zebrafish znfl1s regulate left-right asymmetry patterning through controlling the expression of fgfr1a. J. Cell. Physiol. , 234, 1987-1995. PMID: 30317609 DOI.
- ↑ Naylor RW, Qubisi SS & Davidson AJ. (2017). Zebrafish Pronephros Development. Results Probl Cell Differ , 60, 27-53. PMID: 28409341 DOI.
- ↑ Antinucci P & Hindges R. (2016). A crystal-clear zebrafish for in vivo imaging. Sci Rep , 6, 29490. PMID: 27381182 DOI.
- ↑ Xu PF, Houssin N, Ferri-Lagneau KF, Thisse B & Thisse C. (2014). Construction of a vertebrate embryo from two opposing morphogen gradients. Science , 344, 87-9. PMID: 24700857 DOI.
- ↑ Eames BF, DeLaurier A, Ullmann B, Huycke TR, Nichols JT, Dowd J, McFadden M, Sasaki MM & Kimmel CB. (2013). FishFace: interactive atlas of zebrafish craniofacial development at cellular resolution. BMC Dev. Biol. , 13, 23. PMID: 23714426 DOI.
- ↑ Vesterlund L, Jiao H, Unneberg P, Hovatta O & Kere J. (2011). The zebrafish transcriptome during early development. BMC Dev. Biol. , 11, 30. PMID: 21609443 DOI.
- ↑ Carney TJ, Feitosa NM, Sonntag C, Slanchev K, Kluger J, Kiyozumi D, Gebauer JM, Coffin Talbot J, Kimmel CB, Sekiguchi K, Wagener R, Schwarz H, Ingham PW & Hammerschmidt M. (2010). Genetic analysis of fin development in zebrafish identifies furin and hemicentin1 as potential novel fraser syndrome disease genes. PLoS Genet. , 6, e1000907. PMID: 20419147 DOI.
- ↑ Swinburne IA, Mosaliganti KR, Green AA & Megason SG. (2015). Improved Long-Term Imaging of Embryos with Genetically Encoded α-Bungarotoxin. PLoS ONE , 10, e0134005. PMID: 26244658 DOI.
- ↑ Kague E, Gallagher M, Burke S, Parsons M, Franz-Odendaal T & Fisher S. (2012). Skeletogenic fate of zebrafish cranial and trunk neural crest. PLoS ONE , 7, e47394. PMID: 23155370 DOI.
- ↑ Crump JG, Maves L, Lawson ND, Weinstein BM & Kimmel CB. (2004). An essential role for Fgfs in endodermal pouch formation influences later craniofacial skeletal patterning. Development , 131, 5703-16. PMID: 15509770 DOI.
- ↑ Draper BW, Stock DW & Kimmel CB. (2003). Zebrafish fgf24 functions with fgf8 to promote posterior mesodermal development. Development , 130, 4639-54. PMID: 12925590 DOI.
- ↑ Yan YL, Miller CT, Nissen RM, Singer A, Liu D, Kirn A, Draper B, Willoughby J, Morcos PA, Amsterdam A, Chung BC, Westerfield M, Haffter P, Hopkins N, Kimmel C, Postlethwait JH & Nissen R. (2002). A zebrafish sox9 gene required for cartilage morphogenesis. Development , 129, 5065-79. PMID: 12397114
Journals
Zebrafish "is the only peer-reviewed journal to focus on the zebrafish, which has numerous valuable features as a model organism for the study of vertebrate development. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies, as well as research in toxicology and genomics. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage." [jour PubMed listing]
Reviews
Supatto W & Vermot J. (2011). From cilia hydrodynamics to zebrafish embryonic development. Curr. Top. Dev. Biol. , 95, 33-66. PMID: 21501748 DOI.
Carvalho L & Heisenberg CP. (2010). The yolk syncytial layer in early zebrafish development. Trends Cell Biol. , 20, 586-92. PMID: 20674361 DOI.
Brittijn SA, Duivesteijn SJ, Belmamoune M, Bertens LF, Bitter W, de Bruijn JD, Champagne DL, Cuppen E, Flik G, Vandenbroucke-Grauls CM, Janssen RA, de Jong IM, de Kloet ER, Kros A, Meijer AH, Metz JR, van der Sar AM, Schaaf MJ, Schulte-Merker S, Spaink HP, Tak PP, Verbeek FJ, Vervoordeldonk MJ, Vonk FJ, Witte F, Yuan H & Richardson MK. (2009). Zebrafish development and regeneration: new tools for biomedical research. Int. J. Dev. Biol. , 53, 835-50. PMID: 19557689 DOI.
Bakkers J, Verhoeven MC & Abdelilah-Seyfried S. (2009). Shaping the zebrafish heart: from left-right axis specification to epithelial tissue morphogenesis. Dev. Biol. , 330, 213-20. PMID: 19371733 DOI.
Chan TM, Longabaugh W, Bolouri H, Chen HL, Tseng WF, Chao CH, Jang TH, Lin YI, Hung SC, Wang HD & Yuh CH. (2009). Developmental gene regulatory networks in the zebrafish embryo. Biochim. Biophys. Acta , 1789, 279-98. PMID: 18992377 DOI.
Articles
Warga RM & Kane DA. (2018). A Wilson cell origin for Kupffer's vesicle in the zebrafish. Dev. Dyn. , , . PMID: 30016568 DOI.
Search Pubmed
Search Pubmed: Zebrafish Development
Additional Images
Terms
- deep cell layer - (DEL) formed after blastula stage that forms the three germ layers (ectoderm, mesoderm, and endoderm).
- epiboly - (Greek, "epibol" = a throwing or laying on) Term describing the division and movement of ectodermal cells during gastrulation, thinning and spreading this layer to cover the whole of the embryo. Cellular movements are thought to occur in all vertebrates, but have been most clearly identified in both the zebrafish and frog (xenopus laevis).
- enveloping layer - (EVL) an epithelial monolayer formed after blastula stage that undergoes epiboly.
- Kupffer's vesicle - (ciliated organ of asymmetry, primitive node) a transient epithelial fluid-filled sac located midventrally posterior to the yolk cell or its extension. The vesicle has been described as equivalent to the primitive node for establishing embryo left-right (L-R) axis. PMID 21876750 PMID 30016568
- yolk syncytial layer - (YSL) membrane-enclosed group of nuclei that lie on top of the yolk cell formed after blastula stage that undergoes epiboly.
External Links
External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name. Links to any external commercial sites are provided for information purposes only and should never be considered an endorsement. UNSW Embryology is provided as an educational resource with no clinical information or commercial affiliation.
- NIH NIH Zebrafish Initiative
- ZFIN - The Zebrafish Model Organism Database
- Keller at European Molecular Biology Laboratory, Germany Movies - Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy
- YouTube Timelapse recording of about 18 hours of embryonic development of the zebrafish with some annotation
Online Atlases
- Fish Face Atlas 3D-interactive atlas of craniofacial development in the zebrafish Danio rerio.
- Zebrafish Atlas
- 3D Atlas of Zebrafish Vasculature Anatomy
- Zebrafish Brain Atlas
- Atlas of Zebrafish Anatomy
- Atlas of Zebrafish Development
- Zebrafish Anatomy Portal
- FishNet 3D developmental atlas
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Cite this page: Hill, M.A. (2024, June 5) Embryology Zebrafish Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Zebrafish_Development
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G