|Embryology - 19 Apr 2015 Translate|
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- 1 Introduction
- 2 Some Recent Findings
- 3 Timeline and Stages of Embryonic Development
- 4 Skull
- 5 Molecular
- 6 References
- 7 Additional Images
- 8 Terms
- 9 External Links
- 10 Glossary Links
Zebrafish or zebra danio (danio rerio) are seen as the latest "model' for embryological development studies. These embryos have the great advantage that they develop as "see through" embryos, that is, all internal development can be clearly observed from the outside in the living embryo. Much of the early modern work using this embryo model began with the papers of Kimmel.
Several large laboratories in the US are now developing large breeding programs to carry out "knockouts" and to find spontaneous mutants of interest.
Some Recent Findings
|More recent papers|
References listed on the rest of the content page and the associated discussion page (listed under the publication year sub-headings) do include some editorial selection based upon both relevance and availability.
Qian Jiang, Stacey Arnold, Tiffany Heanue, Krishna Praneeth Kilambi, Betty Doan, Ashish Kapoor, Albee Yun Ling, Maria X Sosa, Moltu Guy, Qingguang Jiang, Grzegorz Burzynski, Kristen West, Seneca Bessling, Paola Griseri, Jeanne Amiel, Raquel M Fernandez, Joke B G M Verheij, Robert M W Hofstra, Salud Borrego, Stanislas Lyonnet, Isabella Ceccherini, Jeffrey J Gray, Vassilis Pachnis, Andrew S McCallion, Aravinda Chakravarti Functional Loss of Semaphorin 3C and/or Semaphorin 3D and Their Epistatic Interaction with Ret Are Critical to Hirschsprung Disease Liability. Am. J. Hum. Genet.: 2015, 96(4);581-596 PMID: 25839327 Hiroki Nagai, Maiko Sezaki, Kisa Kakiguchi, Yukiko Nakaya, Hyung Chul Lee, Raj Ladher, Tomohiro Sasanami, Jae Yong Han, Shigenobu Yonemura, Guojun Sheng Cellular analysis of cleavage-stage chick embryos reveals hidden conservation in vertebrate early development. Development: 2015; PMID: 25742796
[Neurulation continues: the parade commander is...apical constriction]. Ontogenez: 2015, 45(4);240-9 PMID: 25735147
Stephan Heermann, Lucas Schütz, Steffen Lemke, Kerstin Krieglstein, Joachim Wittbrodt Eye morphogenesis driven by epithelial flow into the optic cup facilitated by modulation of bone morphogenetic protein. Elife: 2014, 4; PMID: 25719386 A V Romanovskiĭ, D S Pesnia, E I Izvekov, V V Krylov, V A Nepomniashchikh [The behavior of male Danio rerio after exposure of fish embryos to a simulated geomagnetic storm]. Biofizika: 2015, 59(6);1151-6 PMID: 25715624
Timeline and Stages of Embryonic Development
|0 - 0.75 hrs||Zygote Period|
|0.75 - 2.25 hrs||Cleavage Period|
|2.25 - 5.25 hrs||Blastula Period|
|5.25 - 10.33 hrs||Gastrula Period|
|10.33 - 24 hrs||Segmentation Period|
|24 - 48 hrs||Pharyngula Period|
|48-72 hrs||Hatching Period|
|72 hrs - 30 Days||Larval Period|
- Transition from Prim 5 to Long-pec
- The body axis begins to straighten and the head straightens out and lifts dorsally
- Notochord is well developed
- Formation of the Dorsal and Ventral Stripe
- Nervous system is hollow and expanding anteriorly
- The brain has developed into 5 distinct lobes
- Seven pharyngeal arch's develop rapidly during this stage
- Pectoral fins begin to develop
- The Circulatory system develops and the heart beats for the first time
- Blood begins to circulate through a closed circuit of channels
- Tactile sensitivity appears and uncoordinated movements occur
| Zebrafish Skull Neural Crest Contribution
Fibroblast Growth Factor
- Fgf8 and Fgf3 - regulating the segmentation of the pharyngeal endoderm into pouches. 
- Fgf24 and Fgf8 - promotes posterior mesodermal development.
- Sox9 - required for cartilage morphogenesis.
- C B Kimmel, S K Sessions, R J Kimmel Morphogenesis and synaptogenesis of the zebrafish Mauthner neuron. J. Comp. Neurol.: 1981, 198(1);101-20 PMID: 7229136
- C B Kimmel, D S Sepich, B Trevarrow Development of segmentation in zebrafish. Development: 1988, 104 Suppl;197-207 PMID: 3077108
- Akihiko Muto, Anne L Calof, Arthur D Lander, Thomas F Schilling Multifactorial origins of heart and gut defects in nipbl-deficient zebrafish, a model of Cornelia de Lange Syndrome. PLoS Biol.: 2011, 9(10);e1001181 PMID: 22039349
- Peng-Fei Xu, Nathalie Houssin, Karine F Ferri-Lagneau, Bernard Thisse, Christine Thisse Construction of a vertebrate embryo from two opposing morphogen gradients. Science: 2014, 344(6179);87-9 PMID: 24700857
- B Frank Eames, April DeLaurier, Bonnie Ullmann, Tyler R Huycke, James T Nichols, John Dowd, Marcie McFadden, Mark M Sasaki, Charles B Kimmel FishFace: interactive atlas of zebrafish craniofacial development at cellular resolution. BMC Dev. Biol.: 2013, 13;23 PMID: 23714426
- Liselotte Vesterlund, Hong Jiao, Per Unneberg, Outi Hovatta, Juha Kere The zebrafish transcriptome during early development. BMC Dev. Biol.: 2011, 11;30 PMID: 21609443
- Thomas J Carney, Natália Martins Feitosa, Carmen Sonntag, Krasimir Slanchev, Johannes Kluger, Daiji Kiyozumi, Jan M Gebauer, Jared Coffin Talbot, Charles B Kimmel, Kiyotoshi Sekiguchi, Raimund Wagener, Heinz Schwarz, Phillip W Ingham, Matthias Hammerschmidt Genetic analysis of fin development in zebrafish identifies furin and hemicentin1 as potential novel fraser syndrome disease genes. PLoS Genet.: 2010, 6(4);e1000907 PMID: 20419147
- Kague E, Gallagher M, Burke S, Parsons M, Franz-Odendaal T, et al. (2012) Skeletogenic Fate of Zebrafish Cranial and Trunk Neural Crest. PLoS ONE 7(11): e47394. doi:10.1371/journal.pone.0047394 PLoS ONE
- Justin Gage Crump, Lisa Maves, Nathan D Lawson, Brant M Weinstein, Charles B Kimmel An essential role for Fgfs in endodermal pouch formation influences later craniofacial skeletal patterning. Development: 2004, 131(22);5703-16 PMID: 15509770
- Bruce W Draper, David W Stock, Charles B Kimmel Zebrafish fgf24 functions with fgf8 to promote posterior mesodermal development. Development: 2003, 130(19);4639-54 PMID: 12925590
- Yi-Lin Yan, Craig T Miller, Robert M Nissen, Amy Singer, Dong Liu, Anette Kirn, Bruce Draper, John Willoughby, Paul A Morcos, Adam Amsterdam, Bon-Chu Chung, Monte Westerfield, Pascal Haffter, Nancy Hopkins, Charles Kimmel, John H Postlethwait, Robert Nissen A zebrafish sox9 gene required for cartilage morphogenesis. Development: 2002, 129(21);5065-79 PMID: 12397114
Zebrafish "is the only peer-reviewed journal to focus on the zebrafish, which has numerous valuable features as a model organism for the study of vertebrate development. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies, as well as research in toxicology and genomics. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage." [jour PubMed listing]
Willy Supatto, Julien Vermot From cilia hydrodynamics to zebrafish embryonic development. Curr. Top. Dev. Biol.: 2011, 95;33-66 PMID: 21501748
Lara Carvalho, Carl-Philipp Heisenberg The yolk syncytial layer in early zebrafish development. Trends Cell Biol.: 2010, 20(10);586-92 PMID: 20674361
Sebastiaan A Brittijn, Suzanne J Duivesteijn, Mounia Belmamoune, Laura F M Bertens, Wilbert Bitter, Joost D de Bruijn, Danielle L Champagne, Edwin Cuppen, Gert Flik, Christina M Vandenbroucke-Grauls, Richard A J Janssen, Ilse M L de Jong, Edo Ronald de Kloet, Alexander Kros, Annemarie H Meijer, Juriaan R Metz, Astrid M van der Sar, Marcel J M Schaaf, Stefan Schulte-Merker, Herman P Spaink, Paul P Tak, Fons J Verbeek, Margriet J Vervoordeldonk, Freek J Vonk, Frans Witte, Huipin Yuan, Michael K Richardson Zebrafish development and regeneration: new tools for biomedical research. Int. J. Dev. Biol.: 2009, 53(5-6);835-50 PMID: 19557689
Jeroen Bakkers, Manon C Verhoeven, Salim Abdelilah-Seyfried Shaping the zebrafish heart: from left-right axis specification to epithelial tissue morphogenesis. Dev. Biol.: 2009, 330(2);213-20 PMID: 19371733
Tzu-Min Chan, William Longabaugh, Hamid Bolouri, Hua-Ling Chen, Wen-Fang Tseng, Chung-Hao Chao, Te-Hsuan Jang, Yu-I Lin, Shao-Chin Hung, Horng-Dar Wang, Chiou-Hwa Yuh Developmental gene regulatory networks in the zebrafish embryo. Biochim. Biophys. Acta: 2009, 1789(4);279-98 PMID: 18992377
Search Pubmed: Zebrafish Development
- deep cell layer - (DEL) formed after blastula stage that forms the three germ layers (ectoderm, mesoderm, and endoderm).
- epiboly - (Greek, "epibol" = a throwing or laying on) Term describing the division and movement of ectodermal cells during gastrulation, thinning and spreading this layer to cover the whole of the embryo. Cellular movements are thought to occur in all vertebrates, but have been most clearly identified in both the zebrafish and frog (xenopus laevis).
- enveloping layer - (EVL) an epithelial monolayer formed after blastula stage that undergoes epiboly.
- Kupffer's vesicle - (ciliated organ of asymmetry, primitive node) a transient epithelial fluid-filled sac located midventrally posterior to the yolk cell or its extension. The vesicle has been described as equivalent to the primitive node for establishing embryo left-right (L-R) axis. PMID 21876750
- yolk syncytial layer - (YSL) membrane-enclosed group of nuclei that lie on top of the yolk cell formed after blastula stage that undergoes epiboly.
External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name.
- NIH NIH Zebrafish Initiative
- ZFIN - The Zebrafish Model Organism Database
- Keller at European Molecular Biology Laboratory, Germany Movies - Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy
- YouTube Timelapse recording of about 18 hours of embryonic development of the zebrafish with some annotation
- Fish Face Atlas 3D-interactive atlas of craniofacial development in the zebrafish Danio rerio.
- Zebrafish Atlas
- 3D Atlas of Zebrafish Vasculature Anatomy
- Zebrafish Brain Atlas
- Atlas of Zebrafish Anatomy
- Atlas of Zebrafish Development
- Zebrafish Anatomy Portal
- FishNet 3D developmental atlas
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Cite this page: Hill, M.A. (2015) Embryology Zebrafish Development. Retrieved April 19, 2015, from https://embryology.med.unsw.edu.au/embryology/index.php/Zebrafish_Development
- © Dr Mark Hill 2015, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G