Sheep Development: Difference between revisions
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* '''Healthy ageing of cloned sheep'''<ref name=PMID27459299><pubmed>27459299</pubmed></ref> "The health of cloned animals generated by somatic-cell nuclear transfer (SCNT) has been of concern since its inception; however, there are no detailed assessments of late-onset, non-communicable diseases. Here we report that SCNT has no obvious detrimental long-term health effects in a cohort of 13 cloned sheep. We perform musculoskeletal assessments, metabolic tests and blood pressure measurements in 13 aged (7-9 years old) cloned sheep, including four derived from the cell line that gave rise to Dolly. We also perform radiological examinations of all main joints, including the knees, the joint most affected by osteoarthritis in Dolly, and compare all health parameters to groups of 5-and 6-year-old sheep, and published reference ranges. Despite their advanced age, these clones are euglycaemic, insulin sensitive and normotensive. Importantly, we observe no clinical signs of degenerative joint disease apart from mild, or in one case moderate, osteoarthritis in some animals. Our study is the first to assess the long-term health outcomes of SCNT in large animals." [[Somatic Cell Nuclear Transfer]] | |||
* '''Development of morulae from the oocytes of cultured sheep preantral follicles'''<ref name="PMID20615540"><pubmed>20615540</pubmed></ref> "Sheep preantral follicles (PFs) measuring 250-400 microm in diameter were cultured for six days in serum-free media supplemented differently with growth factors and hormones. ...In combination with T4 and FSH, IGF-I+GH supported the best development of the PFs. Culture of PFs in micro drops or agar gel supported similarly high development. In vitro fertilization of the oocytes from the cultured sheep PFs resulted in the embryos developing to the morula stage for the first time." | * '''Development of morulae from the oocytes of cultured sheep preantral follicles'''<ref name="PMID20615540"><pubmed>20615540</pubmed></ref> "Sheep preantral follicles (PFs) measuring 250-400 microm in diameter were cultured for six days in serum-free media supplemented differently with growth factors and hormones. ...In combination with T4 and FSH, IGF-I+GH supported the best development of the PFs. Culture of PFs in micro drops or agar gel supported similarly high development. In vitro fertilization of the oocytes from the cultured sheep PFs resulted in the embryos developing to the morula stage for the first time." | ||
* '''Viral particles of endogenous betaretroviruses are released in the sheep uterus and infect the conceptus trophectoderm in a transspecies embryo transfer model'''<ref name="PMID20610723"><pubmed>20610723</pubmed></ref> | * '''Viral particles of endogenous betaretroviruses are released in the sheep uterus and infect the conceptus trophectoderm in a transspecies embryo transfer model'''<ref name="PMID20610723"><pubmed>20610723</pubmed></ref> |
Revision as of 16:47, 1 August 2016
Embryology - 26 Apr 2024 Expand to Translate |
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Introduction
The domestic sheep (Ovis aries) has been used as a mammalian model of development.
- term gestational days = 145 days
Some Recent Findings
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More recent papers |
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This table allows an automated computer search of the external PubMed database using the listed "Search term" text link.
More? References | Discussion Page | Journal Searches | 2019 References | 2020 References Search term: Sheep Embryology <pubmed limit=5>Sheep Embryology</pubmed> |
Development Overview
- day 4 - embryo enters the uterus at the morula stage of development
- day 6 - develops into a blastocyst
- day 8 - blastocyst hatches from zona pellucida
- day 11-16 - elongates to a filamentous form
- day 14 - 16 - binucleate cells begin to differentiate in the trophoblast
- day 16 - adplantation
See also Implantation mechanisms: insights from the sheep[5]
Dolly
A female domestic sheep remarkable in being the first mammal to be cloned from an adult somatic cell, using the process of nuclear transfer.[6]
Cloned by Ian Wilmut, Keith Campbell and colleagues at the Roslin Institute near Edinburgh in Scotland, born on 5 July 1996 and she lived until the age of six (5 July 1996 – 14 February 2003). The cell used as the donor for the cloning of Dolly was taken from a mammary gland, and the production of a healthy clone therefore proved that a cell taken from a specific part of the body could recreate a whole individual. As Dolly was cloned from part of a mammary gland, she was named after the famously curvaceous country western singer Dolly Parton.
Oocyte Development
Sheep follicle gene expression[7] |
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Sheep Oocyte Distribution of Telomerase reverse transcriptase
The following oocyte images are from a recent study of sheep in vitro follicle development.[8]
preantral | early antral |
early antral | preovulatory follicle |
- TERT - Red (Cy3-conjugated secondary antibody) (telomerase reverse transcriptase, TERT)
- DNA - Green (SYBR Green 14/I)
- Sheep Oocyte TERT: preantral | early antral | early antral | preovulatory follicle | Oocyte Development | Sheep Development
Respiratory
Phases of fetal lung development:[9]
- embryonic - 0 to 40 days.
- pseudoglandular - 40 to 80 days.
- canalicular - 80 to 120 days.
- saccular - 120 to term 148 days gestation.
- Links: Respiratory Development
Immune
Lymphocytes development has been characterised by an immunohistology study of T lymphocytes in the sheep fetal spleen.[10]
- 43-44 days of gestation, SBU-T1- and SBU-T8-positive lymphocytes were present in low numbers.
- 45-50 days of gestation Surface immunoglobulin (sIg) was first detected on fetal spleen cells
- 50-55 days of gestation SBU-T4, 20.96-, 25.69-, 38.38-, or 46.66-positive lymphocytes present.
- 57 days of gestation SBU-T19 lymphocytes appeared.
- Links: Immune Development
References
- ↑ <pubmed>27459299</pubmed>
- ↑ <pubmed>20615540</pubmed>
- ↑ <pubmed>20610723</pubmed>
- ↑ <pubmed>22132111</pubmed>| PLoS One.
- ↑ <pubmed>15579583</pubmed>
- ↑ <pubmed>9039911</pubmed>
- ↑ <pubmed>26540452</pubmed>| PLoS One.
- ↑ <pubmed>22132111</pubmed>| PLoS One.
- ↑ <pubmed>15774522</pubmed>| PMC1464504
- ↑ <pubmed>3308689</pubmed>
Reviews
<pubmed>19726075</pubmed> <pubmed>16008756</pubmed>
Articles
<pubmed>19909921</pubmed> <pubmed>15047940</pubmed>| Reproduction
Search PubMed
Search Pubmed: sheep development | ovine development | ovine embryo development
External Links
External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name. Links to any external commercial sites are provided for information purposes only and should never be considered an endorsement. UNSW Embryology is provided as an educational resource with no clinical information or commercial affiliation.
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Cite this page: Hill, M.A. (2024, April 26) Embryology Sheep Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Sheep_Development
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G