File:Chicken embryo E-cad and P-cad gastrulation.png

Chicken Embryo Gastrulation and Cadherin Isoforms

This image shows the localisation and changing levels of cadherin during chicken embryo gastrulation HH stage 4, 5 and 7 embryos). Cadherins are transmembrane proteins that act for cell adhesion that require calcium to act ("calcium-dependent adhesion").

The upper panel bright field images (A-F) show in situ hybridisation localisation of different mRNAs during chicken gastrulation. The lefthand panels view the whole embryo and the righthand panels are transverse sections through the embryo.

The lower fluorescent images (G, H, I) show protein localisation during chicken embryo gastrulation.

Green - Slug protein

Red - Cadherin protein, both E-cadherin and P-cadherin isoforms)

(G-I) Immunofluorescence localization of Slug (green) and cadherin proteins using an antibody that recognizes E-cad and P-cad (red) in transverse sections through the middle streak region of HH stage 4, 5 and 7 embryos.

Mark Hill (talk) 12:43, 3 August 2017 (AEST) Below is the original figure legend. Note that there are several unexplained terms and acronyms in the legend title and description that should be fully explained when uploaded and used in your student project.

Localization of E-cadherin and P-cadherin mRNA, and E-cadherin and Slug proteins, in HH stage 4–7 embryos

(A-C, A’-C’) E-cad (CDH1) mRNA expression in HH stage 4, 5 and 7 embryos (A-C) and in transverse sections (A’-C’). (D-F, D’-F’) P-Cad (CDH3) mRNA expression in embryos at stages 4, 5 and 6 (D-F) and in transverse sections (D’-F’). E-cad mRNAs are downregulated in the pre ingression epiblast and in the primitive streak, while P-cad mRNAs persist in the primitive streak and medial mesoderm (arrows). (G-I) Immunofluorescence localization of Slug (green) and cadherin proteins using an antibody that recognizes E-cad and P-cad (red) in transverse sections through the middle streak region of HH stage 4, 5 and 7 embryos.

Reference

<pubmed>27097030</pubmed>

Moly PK, Cooley JR, Zeltzer SL, Yatskievych TA, Antin PB (2016) Gastrulation EMT Is Independent of P-Cadherin Downregulation. PLoS ONE 11(4): e0153591. doi:10.1371/journal.pone.0153591

Copyright

© 2016 Moly et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

http://dx.doi.org/10.1371/journal.pone.0153591.g001

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