File:Chicken embryo E-cad and P-cad gastrulation.png: Difference between revisions

Revision as of 15:04, 12 August 2016

Fig 1. Localization of E-cad and P-cad mRNA, and E-cad and Slug proteins, in HH stage 4–7 embryos.

(A-C, A’-C’) E-cad (CDH1) mRNA expression in HH stage 4, 5 and 7 embryos (A-C) and in transverse sections (A’-C’). (D-F, D’-F’) P-Cad (CDH3) mRNA expression in embryos at stages 4, 5 and 6 (D-F) and in transverse sections (D’-F’). E-cad mRNAs are downregulated in the pre ingression epiblast and in the primitive streak, while P-cad mRNAs persist in the primitive streak and medial mesoderm (arrows). (G-I) Immunofluorescence localization of Slug (green) and cadherin proteins using an antibody that recognizes E-cad and P-cad (red) in transverse sections through the middle streak region of HH stage 4, 5 and 7 embryos.

http://dx.doi.org/10.1371/journal.pone.0153591.g001

PMID 27097030 Moly PK, Cooley JR, Zeltzer SL, Yatskievych TA, Antin PB (2016) Gastrulation EMT Is Independent of P-Cadherin Downregulation. PLoS ONE 11(4): e0153591. doi:10.1371/journal.pone.0153591

Copyright: © 2016 Moly et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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+Fig 1. Localization of E-cad and P-cad mRNA, and E-cad and Slug proteins, in HH stage 4–7 embryos.
+(A-C, A’-C’) E-cad (CDH1) mRNA expression in HH stage 4, 5 and 7 embryos (A-C) and in transverse sections (A’-C’). (D-F, D’-F’) P-Cad (CDH3) mRNA expression in embryos at stages 4, 5 and 6 (D-F) and in transverse sections (D’-F’). E-cad mRNAs are downregulated in the pre ingression epiblast and in the primitive streak, while P-cad mRNAs persist in the primitive streak and medial mesoderm (arrows). (G-I) Immunofluorescence localization of Slug (green) and cadherin proteins using an antibody that recognizes E-cad and P-cad (red) in transverse sections through the middle streak region of HH stage 4, 5 and 7 embryos.
+http://dx.doi.org/10.1371/journal.pone.0153591.g001
 PMID 27097030
+Moly PK, Cooley JR, Zeltzer SL, Yatskievych TA, Antin PB (2016) Gastrulation EMT Is Independent of P-Cadherin Downregulation. PLoS ONE 11(4): e0153591. doi:10.1371/journal.pone.0153591
+Copyright: © 2016 Moly et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.