ANAT2341 Lab 10: Difference between revisions

Revision as of 08:49, 8 October 2018

Organogenesis Lab

1. QUIZ

2. Organogenesis Lab

In this lab you will dissect fertile chicken eggs and study fixed mouse embryos up to mid-gestation using dissection microscopes. You will name the embryonic anatomical structures, and describe what these will give rise to.

Organogensis Lab Manual

These are the Hamburger stages of chicken development

See also the JoVE article on chicken egg preparation: <pubmed>18989413</pubmed>

Additional Chicken Links

Viktor Hamburger (1900 – 2001)

More about chicken embryogenesis: Chicken Development | Hamburger Hamilton Stages

Historic Chicken Embryology
1883 History of the Chick \| 1900 Chicken Embryo Development Plates \| 1904 X-Ray Effects \| 1910 Somites \| 1914 Primordial Germ Cells 1919 Lillie Textbook \| 1920 Chick Early Embryology \| 1933 Neural \| 1939 Sternum \| 1948 Limb \| Movie 1961 \| Historic Papers

stage 1-4
stages 5-10
stages 11-14
stages 15-18
stages 19-21
stages 22-25
stages 26-28
stage 29-32

This figure compares the human and mouse developmental stages

More about Mouse embryogenesis: Mouse Timeline Detailed

chicken

External Links

External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name. Links to any external commercial sites are provided for information purposes only and should never be considered an endorsement. UNSW Embryology is provided as an educational resource with no clinical information or commercial affiliation.

JOVE - An Introduction to the Chicken

@@ Line 1: / Line 1: @@
-=Stem Cell Paper Presentation=
+==Organogenesis Lab==
-==Stem Cell Paper Preparation for Lab 10==
-As part of the assessment for this course, you will give a 15 minutes journal club presentation in the next lab. For this you will in your current student group discuss a recent (published after 2011) original research article (not a review!) on stem cell biology or technology.
+== 1. QUIZ ==
-Please note that the best articles are found in journals with the highest impact factors: Nature, Science, Cell, Cell Stem Cell, etc). Please contact Annemiek in case you are at a loss, and she will help you find one.
+== 2. Organogenesis Lab ==
-During the presentation it works best if one student discusses the introduction, the second the results section, and the third the discussion section. Please note that one slide takes about 1 minute to talk through. So do not use more than 15 to 20 slides total. Please read through attached document for tips for how to prepare a good presentation.
+In this lab you will dissect fertile chicken eggs and study fixed mouse embryos up to mid-gestation using dissection microscopes. You will name the embryonic anatomical structures, and describe what these will give rise to.
-You will receive a group mark based on presentation content, insight and comprehension, and presentation and slide style.
-===Presentation Hints for Students ===
+[[Media:Fertile_Egg_practical_Class_2.pdf|Organogensis Lab Manual]]
-* Do not use cheat sheets and do not learn your presentation literally by heart. '''Make sure that you know and understand what you want to get across.''' Explain carefully. Use your slides as cheat sheets. Make eye contact with your audience and get a feel for whether they understand your story.
+[[File:Chicken_Embryo_Hamburger_stages.jpg|600px|link=Hamburger Hamilton Stages]]
-* '''Keep your presentation short and concise.''' Not every detail of the article needs to be discussed in the presentation, but limit it to the bare minimum that is required to get the main message of the article across. For instance, do not go into too much detail in method sections. Not all nitty-gritty detail of the results needs to be discussed. The less info your audience has to take in, the higher the chance that they will understand your story.
-* Don’t just put your slides up while you are presenting, but talk your audience carefully through them. Slides are an indispensable part of the presentation. '''Each item on your slides should be relevant and addressed and highlighted with pointer, fingers, stick.''' Slide shows are indispensable for a presentation, as is the presenter. They should support and enhance a presentation, they should aid your audience in understanding.
-* '''Talk your audience through each of the figures on your slides.''' Figures may be obvious to you, but not to your audience unless you explain them carefully. So explain what experiment has been carried out, and what is displayed in the figure:
-** on the X and Y-axes
-** what the bars represent in diagrams
-** the tissues/cell types displayed
-** the bands on Western blot, RNA and DNA gels,
-** What colors represent colors in immunostainings, etc etc.
-* Please note that you only need to highlight this experimental detail that is necessary to get the main message of the figure across.
-* Conclude a (results) slide with a concluding/summarizing remark that should cover the main message of this particular slide.
-* Annotate the figures in your presentation carefully but sparingly. Label panels, axes, images etc so that figures are self-explicatory.
-* Do not use too much text on your slides.
-* To stay in control the presenter should flick through the slide show. Not another member of the team.
-* If you didn’t understand the articles in depth, read a recent review or even go back to text books to acquire the basic knowledge. Also, if you discuss results of a crucial experiment but do not understand the technology. Please go back to the original references or your text books to read up on this technology. You should be on top of everything you say or write up in your slides.
-* '''Stick to your time.''' Don’t make too many slides. Each slide should take about a minute on average to talk through. Try to avoid acronyms and abbreviation.
+''These are the Hamburger stages of chicken development''
-{{2016ANAT2341}}
+See also the [https://www.jove.com/video/306/windowing-chicken-eggs-for-developmental-studies JoVE article on chicken egg preparation]: <pubmed>18989413</pubmed>
+===Additional Chicken Links===
+[[File:Viktor Hamburger.jpg|thumb|alt=Viktor Hamburger|link=Embryology History - Viktor Hamburger|Viktor Hamburger (1900 – 2001)]]
+More about chicken embryogenesis: [[Chicken Development]] | [[Hamburger Hamilton Stages]]
+<br>
+{{Chicken links}}
+<br>
+<gallery>
+File:HHstage1-4.jpg|stage 1-4
+File:HHstage5-10.jpg|stages 5-10
+File:HHstage11-14.jpg|stages 11-14
+File:HHstage15-18.jpg|stages 15-18
+File:HHstage19-21.jpg|stages 19-21
+File:HHstage22-25.jpg|stages 22-25
+File:HHstage26-28.jpg|stages 26-28
+File:HHstage29-32.jpg|stage 29-32
+</gallery>
+[[File:Mouse_vs_Human_embryogenesis.jpg]]
+''This figure compares the human and mouse developmental stages''
+More about Mouse embryogenesis: [[Mouse Timeline Detailed]]
+{{Chicken}}
+===External Links===
+{{External Links}}
+* JOVE - [http://www.jove.com/science-education/5153/an-introduction-to-the-chick-gallus-gallus-domesticus An Introduction to the Chicken]
+{{2018ANAT2341}}