File:Chicken embryo E-cad and P-cad gastrulation.png: Difference between revisions

From Embryology
Line 1: Line 1:
==Chicken Embryo Gastrulation and Cadherin Isoforms==
==Chicken Embryo Gastrulation and Cadherin Isoforms==
This image shows the localisation and changing levels of cadherin during chicken embryo gastrulation HH stage 4, 5 and 7 embryos). Cadherins are family of transmembrane proteins (isoforms) that act for cell adhesion that require calcium to act ("calcium-dependent adhesion"). The original paper was able to show that the process of gastrulation epithelial-mesenchymal transition did not require the changing level in P-Cadherin isoform.
[[User:Z8600021|Mark Hill]] ([[User talk:Z8600021|talk]]) 12:43, 3 August 2017 (AEST) The text below has been written by the person who has uploaded the image to the Wiki. It is intended to give a simplified explanation to other students of what the image shows, independent of the original paper context.
 
====Contributor summary====
 
This figure comes from a research paper that was able to show that chicken gastrulation epithelial-mesenchymal transition was independent of (did not require) a changing level in P-Cadherin isoform.
 
This image shows the localisation and changing levels of cadherin during chicken embryo gastrulation HH stage 4, 5 and 7 embryos). Cadherins are family of transmembrane proteins (isoforms) that found at [[:File:Adherens Junction 01.jpg|adherens junctions]] for cell adhesion that also require calcium to act ("calcium-dependent adhesion").  


The naming of the cadherin isoforms is based upon the site of original identification: P-cadherin (placenta cadherin, [https://www.omim.org/entry/114021 cadherin 3]), E-cadherin (epithelial cadherin [https://www.omim.org/entry/192090 cadherin 1]), N-cadherin (neural cadherin, [https://www.omim.org/entry/114020 cadherin 2]).
The naming of the cadherin isoforms is based upon the site of original identification: P-cadherin (placenta cadherin, [https://www.omim.org/entry/114021 cadherin 3]), E-cadherin (epithelial cadherin [https://www.omim.org/entry/192090 cadherin 1]), N-cadherin (neural cadherin, [https://www.omim.org/entry/114020 cadherin 2]).
Line 14: Line 20:




====Original figure legend====


[[User:Z8600021|Mark Hill]] ([[User talk:Z8600021|talk]]) 12:43, 3 August 2017 (AEST) Below is the original figure legend. Note that there are several unexplained terms and acronyms in the legend title and description that should be fully explained when uploaded and used in your student project.
Note that there are several unexplained terms and acronyms in the legend title and description that should be fully explained when uploaded and used in your student project.


Localization of E-cadherin and P-cadherin mRNA, and E-cadherin and Slug proteins, in HH stage 4–7 embryos
Localization of E-cadherin and P-cadherin mRNA, and E-cadherin and Slug proteins, in HH stage 4–7 embryos

Revision as of 13:08, 3 August 2017

Chicken Embryo Gastrulation and Cadherin Isoforms

Mark Hill (talk) 12:43, 3 August 2017 (AEST) The text below has been written by the person who has uploaded the image to the Wiki. It is intended to give a simplified explanation to other students of what the image shows, independent of the original paper context.

Contributor summary

This figure comes from a research paper that was able to show that chicken gastrulation epithelial-mesenchymal transition was independent of (did not require) a changing level in P-Cadherin isoform.

This image shows the localisation and changing levels of cadherin during chicken embryo gastrulation HH stage 4, 5 and 7 embryos). Cadherins are family of transmembrane proteins (isoforms) that found at adherens junctions for cell adhesion that also require calcium to act ("calcium-dependent adhesion").

The naming of the cadherin isoforms is based upon the site of original identification: P-cadherin (placenta cadherin, cadherin 3), E-cadherin (epithelial cadherin cadherin 1), N-cadherin (neural cadherin, cadherin 2).


The upper panel bright field images (A-F) show in situ hybridisation localisation of different mRNAs during chicken gastrulation. The lefthand panels view the whole embryo and the righthand panels are transverse sections through the embryo.

The lower fluorescent images (G, H, I) show protein localisation during chicken embryo gastrulation.

Green - Slug protein
Red - Cadherin protein, both E-cadherin and P-cadherin isoforms)


(G-I) Immunofluorescence localization of Slug (green) and cadherin proteins using an antibody that recognizes E-cad and P-cad (red) in transverse sections through the middle streak region of HH stage 4, 5 and 7 embryos.


Original figure legend

Note that there are several unexplained terms and acronyms in the legend title and description that should be fully explained when uploaded and used in your student project.

Localization of E-cadherin and P-cadherin mRNA, and E-cadherin and Slug proteins, in HH stage 4–7 embryos

(A-C, A’-C’) E-cad (CDH1) mRNA expression in HH stage 4, 5 and 7 embryos (A-C) and in transverse sections (A’-C’). (D-F, D’-F’) P-Cad (CDH3) mRNA expression in embryos at stages 4, 5 and 6 (D-F) and in transverse sections (D’-F’). E-cad mRNAs are downregulated in the pre ingression epiblast and in the primitive streak, while P-cad mRNAs persist in the primitive streak and medial mesoderm (arrows). (G-I) Immunofluorescence localization of Slug (green) and cadherin proteins using an antibody that recognizes E-cad and P-cad (red) in transverse sections through the middle streak region of HH stage 4, 5 and 7 embryos.

Reference

<pubmed>27097030</pubmed>

Moly PK, Cooley JR, Zeltzer SL, Yatskievych TA, Antin PB (2016) Gastrulation EMT Is Independent of P-Cadherin Downregulation. PLoS ONE 11(4): e0153591. doi:10.1371/journal.pone.0153591

Copyright

© 2016 Moly et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

http://dx.doi.org/10.1371/journal.pone.0153591.g001


Note - This image was originally uploaded as part of an undergraduate science student project and may contain inaccuracies in either description or acknowledgements. Students have been advised in writing concerning the reuse of content and may accidentally have misunderstood the original terms of use. If image reuse on this non-commercial educational site infringes your existing copyright, please contact the site editor for immediate removal.

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current15:02, 12 August 2016Thumbnail for version as of 15:02, 12 August 2016320 × 408 (253 KB)Z8600021 (talk | contribs)PMID 27097030

Metadata