File:Chicken embryo E-cad and P-cad gastrulation.png: Difference between revisions

Chicken Embryo Gastrulation and Cadherin Isoforms

Mark Hill (talk) 12:43, 3 August 2017 (AEST) The text below has been written by the person who has uploaded the image to the Wiki. It is intended to give a simplified explanation to other students of what the image shows, independent of the original paper context.

Contributor summary

This figure comes from a research paper that was able to show that chicken gastrulation epithelial-mesenchymal transition (EMT) was independent of (did not require) a changing level in P-Cadherin isoform. Also identified for reference is Slug, a zinc finger transcription factor belonging to the Snail family (SLUG) involved in epithelial-mesenchymal transitions in development.

This image shows the localisation and changing levels of cadherin during chicken embryo gastrulation ([Hamburger Hamilton Stages| Hamburger Hamilton stage 4 to 7 embryos).

Cadherins are family of transmembrane proteins (isoforms) that found at adherens junctions for cell adhesion that also require calcium to act ("calcium-dependent adhesion").

The naming of the cadherin isoforms is based upon the site of original identification: P-cadherin (placenta cadherin, cadherin 3), E-cadherin (epithelial cadherin cadherin 1), N-cadherin (neural cadherin, cadherin 2).

The upper panel bright field images (A-F) show in situ hybridisation localisation of different mRNAs during chicken gastrulation. The lefthand panels view the whole embryo and the righthand panels are transverse sections through the embryo.

The lower fluorescent images (G, H, I) show protein localisation during chicken embryo gastrulation.

Green - Slug protein, (a zinc finger transcription factor belonging to the Snail family, SLUG)

Red - Cadherin protein, both E-cadherin and P-cadherin isoforms)

(G-I) Immunofluorescence localization of Slug (green) and cadherin proteins using an antibody that recognizes E-cad and P-cad (red) in transverse sections through the middle streak region of HH stage 4, 5 and 7 embryos.

Original figure legend

Note that there are several unexplained terms and acronyms in the legend title and description that should be fully explained when uploaded and used in your student project.

Localization of E-cadherin and P-cadherin mRNA, and E-cadherin and Slug proteins, in HH stage 4–7 embryos

(A-C, A’-C’) E-cad (CDH1) mRNA expression in HH stage 4, 5 and 7 embryos (A-C) and in transverse sections (A’-C’). (D-F, D’-F’) P-Cad (CDH3) mRNA expression in embryos at stages 4, 5 and 6 (D-F) and in transverse sections (D’-F’). E-cad mRNAs are downregulated in the pre ingression epiblast and in the primitive streak, while P-cad mRNAs persist in the primitive streak and medial mesoderm (arrows). (G-I) Immunofluorescence localization of Slug (green) and cadherin proteins using an antibody that recognizes E-cad and P-cad (red) in transverse sections through the middle streak region of HH stage 4, 5 and 7 embryos.

Reference

<pubmed>27097030</pubmed>

Moly PK, Cooley JR, Zeltzer SL, Yatskievych TA, Antin PB (2016) Gastrulation EMT Is Independent of P-Cadherin Downregulation. PLoS ONE 11(4): e0153591. doi:10.1371/journal.pone.0153591

Copyright

© 2016 Moly et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

http://dx.doi.org/10.1371/journal.pone.0153591.g001

Note - This image was originally uploaded as part of an undergraduate science student project and may contain inaccuracies in either description or acknowledgements. Students have been advised in writing concerning the reuse of content and may accidentally have misunderstood the original terms of use. If image reuse on this non-commercial educational site infringes your existing copyright, please contact the site editor for immediate removal.