|Embryology - 28 Feb 2017 Expand to Translate|
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|Educational Use Only - Embryology is an educational resource for learning concepts in embryological development, no clinical information is provided and content should not be used for any other purpose.|
The first of prenatal diagnostic tests. Through history there were a number of supposedly diagnostic tests to determine early pregnancy, the most obvious was the absence of an ongoing menstrual cycle in women of reproductive age. It was not until the early 20th century that the first clinical maternal urine test was developed. A complicated test requiring analysis of changes induced in the immature mouse genital system by hormones present in the maternal urine. The test was replaced by a urine test with either the rabbit (Friedman modification) showing similar changes or frog (Xenopus), that could be induced to lay eggs by similar urine effects. In the 1960's an immunological test for human chorionic gonadotropin (hCG) in either maternal urine or blood led to development in the late 1970's of home pregnancy kits that became simple one step tests in the late 1980's.
Note that there are a number of pathological conditions, such as endocrine tumours and hydatidiform moles, that can also mimic the detected hormonal changes.
- Links: Menstrual Cycle | Week 2 | Implantation | Placenta Development | Pregnancy Test | Hydatidiform Mole | Human Chorionic Gonadotropin
Some Recent Findings
|More recent papers|
This table shows an automated computer PubMed search using the listed sub-heading term.
References listed on the rest of the content page and the associated discussion page (listed under the publication year sub-headings) do include some editorial selection based upon both relevance and availability.
Euan Mackay, Christina Dalman, Håkan Karlsson, Renee M Gardner Association of Gestational Weight Gain and Maternal Body Mass Index in Early Pregnancy With Risk for Nonaffective Psychosis in Offspring. JAMA Psychiatry: 2017; PubMed 28241183
Jiwu Lou, Manna Sun, Ying Zhao, Zhisong Ji, Fenghua Liu, Dongzhi Li, Wanfang Xu, Yangyang Lin, Yanhui Liu Rapid and simultaneous detection of common aneuploidies by quadruplex real-time polymerase chain reaction combined with melting curve analysis. PLoS ONE: 2017, 12(2);e0171886 PubMed 28241016
Abbas Jessani, Denise Laronde, Kavita Mathu-Muju, Mario A Brondani Self-Perceived Oral Health and Use of Dental Services by Pregnant Women in Surrey, British Columbia. J Can Dent Assoc: 2016, 82;g28 PubMed 28240578
Sanjita Ravishankar, Alison Migliori, Judy Struminsky, Phinnara Has, C James Sung, Mai He Placental findings in feto-maternal hemorrhage in livebirth and stillbirth. Pathol. Res. Pract.: 2017; PubMed 28238383
L V Madoz, I Prunner, M Jaureguiberry, C-C Gelfert, R L de la Sota, M J Giuliodori, M Drillich Application of a bacteriological on-farm test to reduce antimicrobial usage in dairy cows with purulent vaginal discharge. J. Dairy Sci.: 2017; PubMed 28237589
- 1928 - Bernhard Zondek and Selman Aschheim procedure that measured the increase in gonadotrophic substance in the urine of a pregnant woman.
- Immature mice injection with urine led to ovarian changes after a few days.
- Xenopus clawed frog replaced mice, injection with urine stimulated the females to lay eggs that were easily seen the next day.
- 1960s - immunological tests for chorionic gonadotropin.
- 1972 - Research on hCG at NIH by Judith Vaitukaitis and Griff Ross.
- 1978 - USA first home pregnancy test kits available.
- 1988 - first “one step” test, Clearblue Easy, a blue stripe appeared to indicate pregnancy.
Aschheim-Zondek Test (1928)
Xenopus clawed frogs
Judith Vaitukaitis and Griff Ross (c1971)
Pregnancy Testing in Medicine
The urine test can be an indicator of an existing pregnancy, but does not exclude a pregnancy that has not reached the implantation stage, or even a very early adplantation stage (GA week 3). This test is often used in consultation with information on sexual history and the menstrual cycle, the last menstrual period (LMP).
There are a number of medical procedures and medications that are contraindicated for pregnant women. This can form part of a discussion or screening process prior to the procedure or prescription. This is also an issue in some medical procedures on women of reproductive age that require anaesthesia, see also UK NICE Guideline - section 5.18). A second issue relates to the preoperative pregnancy testing of minors, regulations for this circumstance may differ between countries.
A immunoradiometric assay for human chorionic gonadotropin (hCG) in urine identified that 10% of clinical pregnancies were undetectable on the first day of missed menses. The same test 1 week after the first day of the missed period, the test identified 97% of clinical pregnancies.
- Links: Menstrual Cycle | Abnormal Development - Drugs | Australian Drug Categories | USA Drug Categories
- Aschheim, S., and Zondek, B., Klin. Wschr., 7 8, 1401 (1928).
- Friedman, M.H. Mechanism of ovulation in the rabbit: II. Ovulation produced by the injection of urine from pregnant women. Am J Physiol. 1929 90:617-622. PDF
- Judith L Vaitukaitis Development of the home pregnancy test. Ann. N. Y. Acad. Sci.: 2004, 1038;220-2 PubMed 15838116
- Naomi K Tepper, Polly A Marchbanks, Kathryn M Curtis Use of a checklist to rule out pregnancy: a systematic review. Contraception: 2013, 87(5);661-5 PubMed 23040127
- J F Donaldson, S J Napier, M Ward-Jones, R A Wheeler, P M Spargo Checking pregnancy status in adolescent girls before procedures under general anaesthesia. Arch. Dis. Child.: 2012, 97(10);895-9 PubMed 22447994
- H E Kaplan THE ASCHHEIM-ZONDEK HORMONE TEST FOR PREGNANCY. Cal West Med: 1929, 31(6);412-3 PubMed 18741243
- Committee on Standards and Practice Parameters, Jeffrey L Apfelbaum, Richard T Connis, David G Nickinovich, American Society of Anesthesiologists Task Force on Preanesthesia Evaluation, L Reuven Pasternak, James F Arens, Robert A Caplan, Richard T Connis, Lee A Fleisher, Richard Flowerdew, Barbara S Gold, James F Mayhew, David G Nickinovich, Linda Jo Rice, Michael F Roizen, Rebecca S Twersky Practice advisory for preanesthesia evaluation: an updated report by the American Society of Anesthesiologists Task Force on Preanesthesia Evaluation. Anesthesiology: 2012, 116(3);522-38 PubMed 22273990
- A J Wilcox, D D Baird, D Dunson, R McChesney, C R Weinberg Natural limits of pregnancy testing in relation to the expected menstrual period. JAMA: 2001, 286(14);1759-61 PubMed 11594902
Laurence A Cole The hCG assay or pregnancy test. Clin. Chem. Lab. Med.: 2012, 50(4);617-30 PubMed 22149742
Ann M Gronowski, Mark Cervinski, Ulf-Håkan Stenman, Alison Woodworth, Lori Ashby, Mitchell G Scott False-negative results in point-of-care qualitative human chorionic gonadotropin (hCG) devices due to excess hCGbeta core fragment. Clin. Chem.: 2009, 55(7);1389-94 PubMed 19395437
Brenda G Fahy, Valerie A Gouzd, Joseph N Atallah Pregnancy tests with end-stage renal disease. J Clin Anesth: 2008, 20(8);609-13 PubMed 19100935
K J Catt, M L Dufau, J L Vaitukaitis Appearance of hCG in pregnancy plasma following the initiation of implantation of the blastocyst. J. Clin. Endocrinol. Metab.: 1975, 40(3);537-40 PubMed 1117063
J L Vaitukaitis Changing placental concentrations of human chorionic gonadotropin and its subunits during gestation. J. Clin. Endocrinol. Metab.: 1974, 38(5);755-60 PubMed 4823920
External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name. Links to any external commercial sites are provided for information purposes only and should never be considered an endorsement. UNSW Embryology is provided as an educational resource with no clinical information or commercial affiliation.
- USA - NIH A thin blue line - The History of the Pregnancy Test Kit | Timeline
- USA - Womens Health http://womenshealth.gov/publications/our-publications/fact-sheet/pregnancy-tests.cfm#a Pregnancy tests fact sheet]
- Australia - NSW Health Pregnancy
- UK - National Health Service (NHS) National Institute for Clinical Health and Excellence (NICE) Preoperative Tests The use of routine preoperative tests for elective surgery guidelines. PDF
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Cite this page: Hill, M.A. 2017 Embryology Pregnancy Test. Retrieved February 28, 2017, from https://embryology.med.unsw.edu.au/embryology/index.php/Pregnancy_Test
- © Dr Mark Hill 2017, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G