|Embryology - 23 Nov 2017 Expand to Translate|
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- 1 Introduction
- 2 Some Recent Findings
- 3 Movies
- 4 Oogenesis
- 5 Oocyte Growth
- 6 Meiosis
- 7 Polar Body
- 8 Calcium Release
- 9 Cortical Granules
- 10 Oocyte-Follicle Cell Interaction
- 11 Oocyte Different Species
- 12 Oocyte Metabolism
- 13 Oocyte Protein Expression
- 14 Abnormalities
- 15 Additional Images
- 16 References
- 17 Glossary Links
- 18 Terms
- 19 Glossary Links
Prior to release from the ovary oocytes (eggs, ova) are arrested at an early stage of the first meiotic division as a primary oocyte (primordial follicle). Following puberty, during each menstrual cycle, pituitary gonadotrophin stimulates completion of meiosis 1 the day before ovulation. Early oocytes are also classified as immature (germinal vesicle (GV) or metaphase I (MI) stage). The breakdown of the germinal vesicle indicates a resumption of meiosis and the extrusion of the first polar body (1 PB) indicates completion of the first meiotic division in human oocytes.
- In an adult human female, the development of a primordial follicle containing an oocyte to a preovulatory follicle takes in excess of 120 days.
|Historic Embryology - Oocyte|
|1919 Human Ovum | 1921 The Ovum | 1929 Oocyte Size|
Some Recent Findings
|More recent papers|
This table shows an automated computer PubMed search using the listed sub-heading term.
References listed on the rest of the content page and the associated discussion page (listed under the publication year sub-headings) do include some editorial selection based upon both relevance and availability.
I A Tsakmakidis, M Tsantarliotou, I A Michos, A G Basioura, G Tsousis, C M Boscos Selected steps of swine ICSI method to overcome seasonal effect and achieve acceptable early embryonic development, a preliminary study. Pol J Vet Sci: 2017, 20(3);607-609 PubMed 29166269
Alberto Maria Luciano, Marc-André Sirard Successful in vitro maturation of oocytes: a matter of follicular differentiation. Biol. Reprod.: 2017; PubMed 29165545
Fatma Uysal, Saffet Ozturk, Gokhan Akkoyunlu Superovulation alters DNA methyltransferase protein expression in mouse oocytes and early embryos. J. Assist. Reprod. Genet.: 2017; PubMed 29164502
Zubing Cao, Ronghua Wu, Di Gao, Tengteng Xu, Lei Luo, Yunsheng Li, Jianyong Han, Yunhai Zhang Maternal histone acetyltransferase KAT8 is required for porcine preimplantation embryo development. Oncotarget: 2017, 8(52);90250-90261 PubMed 29163825
Jin-Gu No, Tai-Young Hur, Minghui Zhao, Seunghoon Lee, Mi-Kyung Choi, Yoon-Seok Nam, Dong-Hyun Yeom, Gi-Sun Im, Dong-Hoon Kim Scriptaid improves the reprogramming of donor cells and enhances canine-porcine interspecies embryo development. Reprod Biol: 2017; PubMed 29162325
A human infant ovary histology, showing the large number of oocytes occupying the ovary cortical region. Compare this with a mature ovary and note the absence of any follicle development in the infant. These early oocytes remain at the diplotene stage of the meiosis I during development from fetal life and postnatal childhood, until puberty when the lutenizing hormone (LH) surges stimulate the resumption of meiosis.
The graph below shows the changes in human germ cell numbers in the ovary with age, peaking at about 7 million (occuring in early fetal development) and then decreasing by apopotic cell death. At puberty there remain only about 400,000 and only about 10% of these will be released through reproductive life. (More? Menstrual Cycle)
Human ovary non-growing follicle model
|Human Follicle Classification|
|Follicle class||Alternate nomenclature||Type||Number of Cells||Size (diameter µm)|
|Primordial||small||1, 2, 3||25||less than 50|
| 26 - 100
101 - 300
|up to 200|
|Secondary|| small antral
| 3001 - 500
501 - 1000
| 500 |
1000 - 6000
|Preovulatory||Graafian||8||greater than 1000||greater than 6000|
|Links: Ovary Development | Oocyte Development|
In females, the total number of eggs ever to be produced are present in the newborn female initially arrested at the diplotene stage of the meiosis I from fetal life through childhood until puberty, when the lutenizing hormone (LH) surges stimulate the resumption of meiosis.
- All eggs are arrested at an early stage (prophase I) of the first meiotic division as a primary oocyte (primordial follicle). Following purberty, during each menstrual cycle, pituitary gonadotrophin stimulates completion of meiosis 1 the day before ovulation.
- In meiosis 1, a diploid cell becomes 2 haploid (23 chromosomes) daughter cells, each chromosome has two chromatids. One cell becomes the secondary oocyte the other cell forms the first polar body.
- The secondary oocyte then commences meiosis 2 which arrests at metaphase and will not continue without fertilization.
- At fertilization meiosis 2 completes, forming a second polar body. Note that the first polar body may also undergo this process forming a third polar body.
- Links: Cell Division - Meiosis
The breakdown of the germinal vesicle indicates a resumption of meiosis and the extrusion of the first polar body (1 PB) indicates completion of the first meiotic division in human oocytes. The polar body is a small cytoplasmic exclusion body formed to enclose the excess DNA formed during the oocyte (egg) meiosis and following sperm fertilization. There are 2-3 polar bodies derived from the oocyte present in the zygote, the number is dependent upon whether polar body 1 (the first polar body formed during meiosis 1) divides during meiosis 2. This exclusion body contains the excess DNA from the reductive division (the second and third polar bodies are formed from meiosis 2 at fertilization). These polar bodies do not contribute to the future genetic complement of the zygote, embryo or fetus.
Recent research in some species suggest that the space formed by the peripheral polar body (between the oocyte and the zona pellucia) can influence the site of spermatozoa fertilization.
Polar Body Extrusion Model
The following cartoon model from mouse oocyte study of polar body extrusion, involving cortical cap protrusion and spindle midzone-induced membrane furrowing.
|(A) Chromosomes induce formation of a cortical actomyosin cap/ring prior to polar body extrusion.||(B) Egg activation induces the cortical cap protrusion.||(C) The anaphase spindle midzone induces unilateral furrowing.||(D) Spindle rotation.||(E) Spindle midzone induces bilateral furrowing and abscission of polar body.|
|The squared region of the cortical cap/ring is shown on the top, an actin cap (red) surrounded by a myosin II ring (green).|
Assisted reproductive techniques involving intracytoplasmic sperm injection (ICSI) have looked at the "quality" of the polar body and found that the morphology is related to mature oocyte viability and has the potential to predict oocyte fertilization rates and pregnancy achievement.
- Links: Meiosis
Oocyte calcium ion (Ca2+) release occurs after spermatozoa fusion and is part of the reactivation of meiosis (arrested at metaphase II) and the primary block to polyspermy. Earlier in oocyte meiosis, between prophase I (germinal vesicle stage) and MII, this release mechanism is developed within the cell.
Oocyte cytoplasmic changes include:
- endoplasmatic reticulum reorganization.
- IP3 receptor increase in both number and sensitivity.
- increase in calcium ion concentration.
The release of cortical granules by exocytosis, the "cortical reaction", occurs following spermatozoa fertilisation and is the main block to polyspermy by modifying the zone pellucida. These granules develop from the golgi apparatus initially forming smaller vesicles that coalesce to form mature membrane bound cortical granules (0.2 to 0.6 microns in diameter) located in the cortex of unfertilized oocytes. In mammals, cortical granule production in the developing follicular oocyte is an ongoing and continuous process, with newly synthesized granules translocating to the cortex until the time of ovulation.
- vary in time of initial development between species.
- primordial follicle stage - rat and mouse.
- primary follicle stage - human, monkey, hamsters, and rabbit.
- vary in type formed in the same species.
- migration requires the microfilaments of the cell cytoskeleton.
- are evenly distributed in the cortex of unfertilised oocytes.
- contain carbohydrates, proteinases, ovoperoxidase, calreticulin, N-acetylglucosaminidase
- oocyte-specific member of the astacin family
- mouse cortical granule protease (metalloendoproteases)
- cleaves zona pellucida ZP2
- female mice lacking ovastacin do not cleave ZP2 after fertilisation
Oocyte-Follicle Cell Interaction
The oocyte and the surrounding granulosa cells have a complex paracrine interactions during follicle growth and development. Oocyte maturation has been shown to depend on secretory products of both the granulosa and cumulus cells.
- promotes granulosa cell proliferation in preantral and antral follicles (GDF-9, BMP15)
- cumulus expansion and granulosa cell differentiation are dependent upon oocyte-derived factors
- BMP15 inhibits FSH-stimulated progesterone production
Oocyte Different Species
|1929 Hartman - Species Oocyte Sizes|
|Animal||Most probable size of egg (μm)||Jenkinson's Table|
|Echidna||3 .0 mm|
Table 8 Reference: Hartman CG. How Large is the Mammalian Egg?: A Review. (1929) Quart. Rev. Biol., 4(3): 373-388.
In the mouse, the secondary follicle stage through to large antral follicle stage the oocyte has a highly oxidative metabolism. In contrast, the surrounding surrounding granulosa and cumulus cells are highly glycolytic. In this second group, the cumulus cells are found to be more glycolytic than the granulosa cells.
- oxidative metabolism - requires oxygen (aerobic) to make energy from carbohydrates (sugars) into pyruvate that passes into the mitochondria where it is fully oxidised by oxygen into carbon dioxide. Also called aerobic metabolism, aerobic respiration, and cell respiration.
- glycolytic metabolism - requires no oxygen (anaerobic) to make energy from carbohydrates (sugars) into pyruvate that is reduced by adenine dinucleotide hydride (NADH).
In the cat oocyte, in pre-antral oocytes mitochondria have a homogeneous distribution throughout the cytoplasm. In the antral stage they have relocated to a mainly pericortical distribution.
- Links: OMIM SIRT1
Oocyte Protein Expression
The table above shows the pattern of protein expression (as percentages of total) in the mouse germinal vesicle and MII oocyte according to 14 molecular function categories.
- Links: Germinal vesicle oocyte protein expression | MII oocyte protein expression | Zygote Protein Expression | Mouse Development | Oocyte Development | Zygote
Oocyte Telomerase Reverse Transcriptase
There is a redistribution of the enzyme that regulates telomere length during oocyte development. The following oocyte images are from a recent study of sheep in vitro follicle development.
|early antral||preovulatory follicle|
- TERT - Red (Cy3-conjugated secondary antibody) (telomerase reverse transcriptase, TERT)
- DNA - Green (SYBR Green 14/I)
- Sheep Oocyte TERT: preantral | early antral | early antral | preovulatory follicle | Oocyte Development | Sheep Development
Meiotic non-disjunction resulting in aneuploidy, most are embryonic lethal and not seen. The potential for genetic abnormalities increase with maternal age.
|Autosomal chromosome aneuploidy||Sex chromosome aneuploidy|
|Historic Disclaimer - information about historic embryology pages|
|Embryology History | Historic Embryology Papers)|
Hamilton WJ. Phases of Maturation and Fertilization in Human Ova. (1944) J Anat. 78:1-4.
Pincus G. and Enzmann EV. The Comparative Behavior of Mammalian Eggs in Vivo and in Vitro. (1935) J Exp Med. 62(5):665-75. PMID 19870440
- Ana Sílvia Pires-Luís, Eduardo Rocha, Carla Bartosch, Elsa Oliveira, Joaquina Silva, Alberto Barros, Rosália Sá, Mário Sousa A stereological study on organelle distribution in human oocytes at prophase I. Zygote: 2015;1-9 PubMed 26170179 | Zygote.
- Yvonne A R White, Dori C Woods, Yasushi Takai, Osamu Ishihara, Hiroyuki Seki, Jonathan L Tilly Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women. Nat. Med.: 2012, 18(3);413-21 PubMed 22366948
- W Hamish B Wallace, Thomas W Kelsey Human ovarian reserve from conception to the menopause. PLoS ONE: 2010, 5(1);e8772 PubMed 20111701
- Jeanine Griffin, Benjamin R Emery, Ivan Huang, C Matthew Peterson, Douglas T Carrell Comparative analysis of follicle morphology and oocyte diameter in four mammalian species (mouse, hamster, pig, and human). J. Exp. Clin. Assist. Reprod.: 2006, 3;2 PubMed 16509981 | J Exp Clin Assist Reprod.
- Qiong Wang, Catherine Racowsky, Manqi Deng Mechanism of the chromosome-induced polar body extrusion in mouse eggs. Cell Div: 2011, 6;17 PubMed 21867530 | PMC3179692 | Cell Div.
- T Ebner, C Yaman, M Moser, M Sommergruber, O Feichtinger, G Tews Prognostic value of first polar body morphology on fertilization rate and embryo quality in intracytoplasmic sperm injection. Hum. Reprod.: 2000, 15(2);427-30 PubMed 10655316
- Johnny S Younis, Orit Radin, Ido Izhaki, Moshe Ben-Ami Does first polar body morphology predict oocyte performance during ICSI treatment? J. Assist. Reprod. Genet.: 2009, 26(11-12);561-7 PubMed 19960239 | PMC2799563
- Anna D Burkart, Bo Xiong, Boris Baibakov, Maria Jiménez-Movilla, Jurrien Dean Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy. J. Cell Biol.: 2012, 197(1);37-44 PubMed 22472438
- Lorena Aguilar-Arnal, Suman Ranjit, Chiara Stringari, Ricardo Orozco-Solis, Enrico Gratton, Paolo Sassone-Corsi Spatial dynamics of SIRT1 and the subnuclear distribution of NADH species. Proc. Natl. Acad. Sci. U.S.A.: 2016; PubMed 27791113
- Rachel Cinco, Michelle A Digman, Enrico Gratton, Ulrike Luderer Spatial Characterization of Bioenergetics and Metabolism of Primordial to Preovulatory Follicles in Whole Ex Vivo Murine Ovary. Biol. Reprod.: 2016; PubMed 27683265
- N Songsasen, L H Henson, W Tipkantha, C Thongkittidilok, J H Henson, K Chatdarong, P Comizzoli Dynamic changes in mitochondrial DNA, distribution and activity within cat oocytes during folliculogenesis. Reprod. Domest. Anim.: 2017; PubMed 28111812
- Shufang Wang, Zhaohui Kou, Zhiyi Jing, Yu Zhang, Xinzheng Guo, Mengqiu Dong, Ian Wilmut, Shaorong Gao Proteome of mouse oocytes at different developmental stages. Proc. Natl. Acad. Sci. U.S.A.: 2010, 107(41);17639-44 PubMed 20876089 | PNAS
- Barbara Barboni, Valentina Russo, Sandra Cecconi, Valentina Curini, Alessia Colosimo, Maria Luigia A Garofalo, Giulia Capacchietti, Oriana Di Giacinto, Mauro Mattioli In vitro grown sheep preantral follicles yield oocytes with normal nuclear-epigenetic maturation. PLoS ONE: 2011, 6(11);e27550 PubMed 22132111 | PLoS One.
Rong Li, David F Albertini The road to maturation: somatic cell interaction and self-organization of the mammalian oocyte. Nat. Rev. Mol. Cell Biol.: 2013, 14(3);141-52 PubMed 23429793
Min Liu The biology and dynamics of mammalian cortical granules. Reprod. Biol. Endocrinol.: 2011, 9;149 PubMed 22088197
- A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols
- antral follicle - (secondary) the stage following preantral in the decription of the sequence ovarian follicle development.
- antrum - (L. a cave), cavity; a nearly-closed cavity or bulge. In the ovary this refers to the follicular fluid-filled space within the follicle.
- atretic follicle - An ovarian follicle that fails to mature and degenerates. Also called "atresia" refering to the process of degeneration of the ovarian follicle. This process can occur at any stage of follicle development (folliculogenesis).
- clomiphene citrate - drug taken orally to promote the process of follicle/egg maturation.
- corona radiata - Layer of follicle cells of cumulus oophorus remaining attached to zona pellucida of oocyte after ovulation. Also called granulosa cells.
- corpus albicans - (L. corpus = body, L. albicans = whitish); a degenerating corpus luteum in ovary.
- corpus luteum - (L. corpus = body, L. luteum = yellow) The remains of ovarian follicle after ovulation that acts as an endocrine organ supporting pregnancy and preventing menstruation (loss of the endometrial lining). de Graaf first observed it in the ovary of a cow as a yellow structure.
- cortical - (L. corticalis) at the outside (like the bark of a tree), usually combined with medulla meaning the core.
- cumulus oophorus - (L. cumulus = a little mound G. oon = egg + phorus = bearing); part of the wall of an ovarian follicle surrounding and carrying the ovum (oocyte).
- follicle - (L. folliculus = little bag,dim. of L. follis). A structure which develops in the ovary and contains a developing egg (oocyte).
- follicle stimulating hormone - (FSH, gonadotropin) A glycoprotein hormone secreted by anterior pituitary (adenohypophysis gonadotrophs, a subgroup of basophilic cells) and acts on gametogenesis and other systems in both males and females. Females, FSH acts on the ovary to stimulate follicle development. Males, acts on the testis Sertoli cells to increase androgen-binding protein (ABP) that binds androgens and has a role in spermatogenesis.
- follicular fluid - the fluid found in the antrum of a secondary follicle. Secreted by cells in the wall of the follicle. This fluid is released along with the oocyte at ovulation.
- germinal epithelium - cellular component covering surface of ovary, it is continuous with mesothelium covering mesovarium. Note that it is a historical misnomer, as it is not the actual site of germ cell formation.
- Graafian follicle - named after Regnier de Graaf (1641-1673), an historic Dutch physician embryologist who studied pregnancy using rabbits.
- granulosa cells - the supporting cells that surround the developing egg within the follicle thecal layers.
- Izumo1 - a protein located on the equatorial segment of acrosome-reacted spermatozoa recognizes its receptor Juno, on the oocyte surface, for plasma membrane binding and fusion. Named for a Japanese shrine dedicated to marriage. OMIM609278
- Juno - (folate receptor-δ; FOLR-δ) a glycophosphatidylinositol (GPI)-anchored, cysteine-rich glycoprotein on the oocyte surface for fertilisation that is the receptor of Izumo1 on the spermatozoa, for plasma membrane binding and fusion. OMIM615737
- luteinizing hormone - (LH, gonadotropin, lutropin, Interstitial Cell Stimulating Hormone, ICSH) glycoprotein hormone releasd from anterior pituitary hormone that acts on the gonad and has a role in male and female reproduction. Female, LH triggers ovulation (release of the oocyte). Male, LH stimulates testis interstital cell (Leydig cell) production of testosterone. Have been used clinically in humans for the treatment of female infertility.
- mesovarium - mesentry of the ovary formed from a fold of the broad ligament that attaches the ovary.
- medullary - (L. medius = in the middle) relating to the medulla; pith, marrow, inner portion of an organ. Usually combined with cortex (cortical) meaning the outer layer.
- oocyte - (Greek, oo = egg, ovum) The term used to describe the haploid egg or ovum formed within the ovary (female gonad) and released to enter the uterine tube and be transported to the uterus. The mature oocyte is the cell released from the ovary during ovulation.
- oocyte retrieval - (egg retrieval) A clinical in vitro fertilization (IVF) procedure to collect the eggs contained in the ovarian follicles.
- oogenesis - (Greek, oo = egg + genesis = origin, creation, generation) process of diploid oogonia division and differentiation into an haploid oocyte (egg) within the ovary (female gonad). Mammalian meiosis will only be completed within the oocyte if fertilization occurs.
- oogonia - (Greek, oo = egg) diploid germ cells within the ovary (female gonad) which provide the primary oocytes for oocyte (egg) formation. In humans, all oogonia form primary oocytes within the ovary before birth.
- oolemma - (zona pellucida, vitelline membrane).
- oophorus - (Greek, oo = egg + phorus = carrying, egg-bearing) cumulus oophorus, used to describe the granulosa cells within the follicle that tether or link the oocyte to the wall of the follicle.
- ovulation - release of the oocyte from the mature follicle. In humans generally a single oocyte is released from a cohort of several maturing follicles.
- preantral follicle - (primary) the stage following primordial in the description of the sequence ovarian follicle development.
- primary follicle - (preantral) the stage following primordial in the description of the sequence ovarian follicle development.
- primordial follicle - the first stage in the description of the sequence ovarian follicle development. Present in the ovary from birth, located in the stroma of the ovary cortex beneath the tunica albuginea. The primordial follicle is the oocyte and the surrounding follicular cells.
- primordial germ cell - oocyte present in the primordial follicle ovary from birth, located in the stroma of the ovary cortex beneath the tunica albuginea. The primordial follicle is the oocyte and the surrounding follicular cells.
- secondary follicles - the stage following primary in the description of the sequence ovarian follicle development.
- stromal cells - in the ovary, cells surrounding the developing follicle that form a connective tissue sheath (theca folliculi). This layer then differentiates into 2 layers (theca interna, theca externa). This region is richly vascularized and involved in hormone secretion.
- superovulation therapy - a fertility drug treatement (oral clomiphene citrate and/or injectable FSH with or without LH) aimed at stimulating development/release of more than one follicle during a single menstrual cycle.
- tertiary follicle - (preovulatory, Graffian) the stage following secondary in the description of the sequence ovarian follicle development.
- theca folliculi - stromal cells in the ovary, cells surrounding the developing follicle that form a connective tissue sheath. This layer then differentiates into 2 layers (theca interna, theca externa). This region is vascularized and involved in hormone secretion.
- theca externa - stromal cells forming the outer layer of the theca folliculi surrounding the developing follicle. Consisting of connective tissue cells, smooth muscle and collagen fibers.
- theca interna - stromal cells forming the inner layer of the theca folliculi surrounding the developing follicle. This vascularized layer of cells respond to LH (leutenizing hormone) synthesizing and secreting androgens which are processed into estrogen.
- transzonal projection - (TZP) ovarian follicle term describing the cellular membraneous extension from the granulosa cell through the zona pellucida to the oocyte cell membrane where it forms gap junctions or adherens junctions allowing signalling and adhesion between the two cells.
- tunica albuginea - dense connective tissue layer lying between germinal epithelium and cortical region of ovary.
- uterus - site of embryo implantation and development. Uterine wall has 3 major layers: endometrium, myometrium, and perimetrium. Endometrium can be further divided into the functional layer (shed/lost during menstruation) and basal layer (not lost during menstruation).
- zona pellucida - extracellular layer lying directly around the oocyte underneath follicular cells. Has an important role in egg development, fertilization and blastocyst development. This thick extracellular matrix consists of glcosaminoglycans and 3 glycoproteins (ZP1, ZP2, ZP3). (More? Zona pellucida)
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Cite this page: Hill, M.A. 2017 Embryology Oocyte Development. Retrieved November 23, 2017, from https://embryology.med.unsw.edu.au/embryology/index.php/Oocyte_Development
- © Dr Mark Hill 2017, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G