Oocyte Meiosis Movie 1

Embryology - 11 Dec 2023 Expand to Translate
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<html5media height="400" width="800">File:Oocyte_Meiosis_01.mp4</html5media>

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A mouse oocyte undergoing spindle migration followed by first polar body extrusion and MII spindle positioning.

blue - Hoechst staining of chromosomes.
green - F-actin probe (UtrCH-GFP, utrophin calponin homology domain fused to GFP) label cortical changes during spindle migration.

The video shows that cytoplasmic streaming continues to the MII arrest stage to maintain the oocyte set of chromosomes/MII spindle in place close to the cortex.

Frames are 11 min apart, and video length is 840 min. Bar, 20 µm.

Meiosis Links: Oocyte Meiosis Movie | 0 min | 270 min | 280 min | 320 min | 840 min | Meiosis spindle movement model | oocyte | meiosis | mouse | Movies

Reference

Yi K, Rubinstein B, Unruh JR, Guo F, Slaughter BD & Li R. (2013). Sequential actin-based pushing forces drive meiosis I chromosome migration and symmetry breaking in oocytes. J. Cell Biol. , 200, 567-76. PMID: 23439682 DOI.

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Video 7 Original video altered in size and movie format.

Cite this page: Hill, M.A. (2023, December 11) Embryology Oocyte Meiosis Movie 1. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Oocyte_Meiosis_Movie_1

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