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2015 Course: Week 2 Lecture 1 Lecture 2 Lab 1 | Week 3 Lecture 3 Lecture 4 Lab 2 | Week 4 Lecture 5 Lecture 6 Lab 3 | Week 5 Lecture 7 Lecture 8 Lab 4 | Week 6 Lecture 9 Lecture 10 Lab 5 | Week 7 Lecture 11 Lecture 12 Lab 6 | Week 8 Lecture 13 Lecture 14 Lab 7 | Week 9 Lecture 15 Lecture 16 Lab 8 | Week 10 Lecture 17 Lecture 18 Lab 9 | Week 11 Lecture 19 Lecture 20 Lab 10 | Week 12 Lecture 21 Lecture 22 Lab 11 | Week 13 Lecture 23 Lecture 24 Lab 12 | 2015 Projects: Three Person Embryos | Ovarian Hyper-stimulation Syndrome | Polycystic Ovarian Syndrome | Male Infertility | Oncofertility | Preimplantation Genetic Diagnosis | Students | Student Designed Quiz Questions | Moodle page

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--Mark Hill (talk) 10:47, 6 August 2015 (AEST) Thanks for setting up your page. We will be talking more about this in the Practical on Friday.

Lab Attendance

--Z3251292 (talk) 13:46, 7 August 2015 (AEST)

--Mark Hill (talk) 17:40, 7 August 2015 (AEST) Note Square brackets not curly for links. Curly brackets are for templates.

--Z3251292 (talk) 15:43, 10 August 2015 (AEST)Many Thanks

--Z3251292 (talk) 13:13, 14 August 2015 (AEST)

Test student 2015

Week2 Lab1 Reference

Summary 1

Casillas evaluated the in-vitro maturation, and in-vitro fertilization of cryopreserved immature porcine oocyte with different protocols. Immature porcine oocytes were vitrified with 7.5% dimethylsulphoxide (Me2SO) and 7.5% ethylene glycol (EG), followed by preserving with the cryolock protocol. Two test groups of cryopreserved oocytes are the cumulus-cell oocyte complexes (COCs group), and the denuded oocytes co-cultured with granulose cells (NkO-cc group). The un-vitrified fresh oocytes were used as the control group in experiments.

The in-vitro maturation was achieved by culturing in maturation medium supplemented with Luteinizing hormone(LH) and Follicle-stimulating hormone(FSH). Then the matured oocytes were subjected to in-vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). The capabilities of embryo development of the test oocytes were compared.

Statistical results showed that the NKO-cc group produced higher cleavage rate and blastocyst production than the COCs group, which also suggested a higher embryonic development in NKO-cc group. And there was no significant difference between the control group and the NKO-cc group. Blastocysts were generated by both IVF and ISCI, Whilst IVF resulted in better blastocyst development.

Reference

Summary 2

Oxidantive stress caused by high levels of reactive oxygen species (ROS) production is believed to degrade spermatozoa generically and functionally. Crocin, which can quench free radicals were hypothesised to serve as an anti-oxidant protector, so as to improve the quality of sperm, and the in vitro fertilization rate.

Sapanidou investigated whether the addition of Crocin in in-vitro sperm preparation media will improve the sperm quality by preventing them over-oxidated during the freeze-thaw process. Spermatozoa collected from 4 bulls were freezed, thawed and washed, followed by incubation with media supplemented with 3 different concentration of crocin (0.5, 1 and 3mM) up to 240 minutes. The sperm motility, viability, acrosomal status, DNA fragmentation index, intracellular ROS, and lipid peroxidation were evaluated. The author also tested the effects of crocin (1mM) in the IVF medium by evaluating the embryo development rate.

Results showed that spermatozoa incubated with 1mM crocin developed lower level of ROS production, lower lipid peroxidant and lower percentage of fragmented cells. They also maintained better motility, viability, and acrosomal function. Furthermore, the addition of 1mM crocin in IVF media significantly increased the embryo development rate. Thus, the author concluded that 1mM crocin improved the sperm quality and fertilization rate by regulating ROS concentration.

Reference

Week3 Lab2 Image

Embryo and Uterus stained with CD34 at Embryonic day 6. Impaired formation of decidual angiogenesis has been detected at E6. N:normal; FD:folate-Deficient group; E:Embryo; L:luminal epithelium; G:glandular epthelium; M:mesometrial; AM:anti-mesometrial; VSF:vascular sinus folding. Scale bar: 500um(left), 100um(right). Embryo and Uterus stained with CD34 at Embryonic day 6

PMID 26247969 This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Uploading Images in 5 Easy Steps

First Read the help page Images and Copyright Tutorial. Hint - This exercise is best done by using separate tabs on your browser so that you can keep all the relevant pages easily available. You can also use your own discussion page to copy and paste links, text. PMIDs etc that you will need in this process. Find an image . Search PubMed using an appropriate search term. Note that there is a special library of complete (full online) article and review texts called PubMed Central (PMC). Be very careful, while some of these PMC papers allow reuse, not all do and to add the reference link to your image you will still need to use the PMID. You can also make your own search term. In this link example PMC is searched for images related to "embryo+implantation" http://www.ncbi.nlm.nih.gov/pmc/?term=embryo+implantation&report=imagesdocsum. simply replace "embryo+implantation" with your own search term, but remember not everything in PMC can be reused, you will still need to find the "copyright notice" on the full paper, no notice, no reuse. Where else can I look? BioMed Central is a separate online database of journals that allow reuse of article content. Also look at the local page Journals that provides additional resources. You have found an image, go to step 2. Check the Copyright. I cannot emphasise enough the importance of this second step. The rule is unless there is an obvious copyright statement that clearly allows reuse (there are several different kinds of copyright, some do not) located in the article or on the article page, move on and find another resource. Not complying with this is a serious academic infringement equivalent to plagiarism."Plagiarism at UNSW is defined as using the words or ideas of others and passing them off as your own." (extract from UNSW statement on Academic Honesty and Plagiarism) You have found the statement and it allows reuse, go to step 3. Downloading your image. Download the image to your own computer. Either use the download image on the page or right click the image. To find the downloaded image you may have to look in your computer downloads folder, or the default location for downloaded files. The image file will have its own original name, that you will not be using on the wiki. You can rename it now (see renaming below), but you should also make a note of the original name. Make sure you have everything ready then for the You have the image file on your computer, go to step 4. Uploading your image. First make sure you have all the information you want to use with the file readily available. There is also a detailed description below. Towards the bottom of the lefthand menuunder “Toolbox” click Upload file. This will open a new window. In the top window "Source file", click "Choose file" and then navigate to find the file on the computer. and select the image. If you have done this correctly the upload window will now have your image file shown in choose file and also in the lower window "File description" in "Destination filename:" DO NOT CLICK UPLOAD FILE YET. Rename your file in "Destination filename:" this should be a brief filename that describes the image. Not any of the following - the original file name, image, file, my image, your ZID, etc. Many of the common embryology names may have already been used, but you can add a number (01, 02, 03, etc) or the PMID number to the filename to make it unique. If the filename or image has already been used or exists it will be shown on the upload page. If another student has already uploaded that image you will have to find another file. Duplicated images will not receive a mark, so check before you upload as you cannot delete images. In the "Summary" window for now just paste the PMID. You will come back and edit this information. Now click "Upload image" at the bottom of the window, go to step 4. Edit and Add to your page. Edit - Open the image with the "Edit" tab at the top of its page. You should see the PMID you had pasted earlier in the new edit window. Add the following information to the summary box. Image Title as a sub-heading. Under this title add the original figure legend or your own description of the image. Image Reference sub-sub-heading. Use the PMID link method shown in Lab 1 and you can also have a direct link to the original Journal article. Image Copyright sub-sub-heading. Add the copyright information under this sub-sub-heading exactly as shown in the original paper. Student Image template, as shown here {{Template:Student Image}} to show that it is a student uploaded image. Add - Now add your image to your own page under a subheading for Lab 2 Assessment including a description and a reference link. If still stuck with this last step, look at the example on the Test Student page. Done! Students cannot delete images once uploaded. You will need to email me with the full image name and request deletion, that I am happy to do with no penalty if done before I assess. Non-Table version of this page Stress Relief.... <html5media height="480" width="640">http://www.youtube.com/watch?v=i9Hwn2DOgKo</html5media>