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--Mark Hill (talk) 10:47, 6 August 2015 (AEST) Thanks for setting up your page. We will be talking more about this in the Practical on Friday.

Lab Attendance

--Z3251292 (talk) 13:46, 7 August 2015 (AEST)

--Mark Hill (talk) 17:40, 7 August 2015 (AEST) Note Square brackets not curly for links. Curly brackets are for templates.

--Z3251292 (talk) 15:43, 10 August 2015 (AEST)Many Thanks

Test student 2015

Week2 Lab Assessment

Summary 1

Casillas ^[1] evaluated the in-vitro maturation, and in-vitro fertilization of cryopreserved immature porcine oocyte with different protocols. Immature porcine oocytes were vitrified with 7.5% dimethylsulphoxide (Me2SO) and 7.5% ethylene glycol (EG), followed by preserving with the cryolock protocol. Two test groups of cryopreserved oocytes are the cumulus-cell oocyte complexes (COCs group), and the denuded oocytes co-cultured with granulose cells (NkO-cc group). The un-vitrified fresh oocytes were used as the control group in experiments.

The in-vitro maturation was achieved by culturing in maturation medium supplemented with LH and FSH. Then the matured oocytes were subjected to in-vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). The capabilities of embryo development of the test oocytes were compared.

Statistical results showed that the NKO-cc group produced higher cleavage rate and blastocyst production than the COCs group, which also suggested a higher embryonic development in NKO-cc group. And there was no significant difference between the control group and the NKO-cc group. Blastocysts were generated by both IVF and ISCI, Whilst IVF resulted in better blastocyst development.

Reference

↑ <pubmed>26254037</pubmed>

Summary 2

Oxidantive stress caused by high levels of reactive oxygen species (ROS) production is believed to degrade spermatozoa generically and functionally. Crocin, which can quench free radicals were hypothesised to serve as an anti-oxidant protector, so as to improve the quality of sperm, and the in vitro fertilization rate.

Sapanidou, etc.^[1] investigated whether the addition of Crocin in in-vitro sperm preparation media will improve the sperm quality by preventing them over-oxidated during the freeze-thaw process. Spermatozoa collected from 4 bulls were freezed, thawed and washed, followed by incubation with media supplemented with 3 different concentration of crocin (0.5, 1 and 3mM) up to 240 minutes. The sperm motility, viability, acrosomal status, DNA fragmentation index, intracellular ROS, and lipid peroxidation were evaluated. The author also tested the effects of crocin (1mM) in the IVF medium by evaluating the embryo development rate.

Results showed that spermatozoa incubated with 1mM crocin developed lower level of ROS production, lower lipid peroxidant and lower percentage of fragmented cells. They also maintained better motility, viability, and acrosomal function. Furthermore, the addition of 1mM crocin in IVF media significantly increased the embryo development rate. Thus, the author concluded that 1mM crocin improved the sperm quality and fertilization rate by regulating ROS concentration.

Reference

↑ <pubmed>26253435</pubmed>

Week3 Lab Assessment

Talk

Timeline of The artificial reproductive technology revolution

1984 donor egg pregnancies
1984 frozen embryos
1960 Ovulation induction
1960 Donor sperm
1978 IVF
1988 Pre-implantation genetic diagnosis
1992 Sperm injection
1994 Blastocyst development
1998 Reliable sperm sex selection
2000 Stem cells and cloning and gene transfer
2005 Ovarian reserve testing
2008 Oocyte freezing, social freezing
2013 Whole genome screenning of embryos

[1] <pubmed>26254037</pubmed>

[2] <pubmed>26253435</pubmed>

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