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2015 Course: Week 2 Lecture 1 Lecture 2 Lab 1 | Week 3 Lecture 3 Lecture 4 Lab 2 | Week 4 Lecture 5 Lecture 6 Lab 3 | Week 5 Lecture 7 Lecture 8 Lab 4 | Week 6 Lecture 9 Lecture 10 Lab 5 | Week 7 Lecture 11 Lecture 12 Lab 6 | Week 8 Lecture 13 Lecture 14 Lab 7 | Week 9 Lecture 15 Lecture 16 Lab 8 | Week 10 Lecture 17 Lecture 18 Lab 9 | Week 11 Lecture 19 Lecture 20 Lab 10 | Week 12 Lecture 21 Lecture 22 Lab 11 | Week 13 Lecture 23 Lecture 24 Lab 12 | 2015 Projects: Three Person Embryos | Ovarian Hyper-stimulation Syndrome | Polycystic Ovarian Syndrome | Male Infertility | Oncofertility | Preimplantation Genetic Diagnosis | Students | Student Designed Quiz Questions | Moodle page

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--Mark Hill (talk) 10:47, 6 August 2015 (AEST) Thanks for setting up your page. We will be talking more about this in the Practical on Friday.

Lab Attendance

--Z3251292 (talk) 13:46, 7 August 2015 (AEST)

--Mark Hill (talk) 17:40, 7 August 2015 (AEST) Note Square brackets not curly for links. Curly brackets are for templates.

--Z3251292 (talk) 15:43, 10 August 2015 (AEST)Many Thanks

Test student 2015

Week2 Lab Assessment

Summary 1

Casillas's study aimed to evaluate the in-vitro maturation, and in-vitro fertilization of cryopreserved immature porcine oocyte. Immature porcine oocytes were vitrified with 7.5% dimethylsulphoxide (Me2SO) and 7.5% ethylene glycol (EG), followed by preserving with the cryolock protocol. Two test groups of cryopreserved oocytes are the cumulus-cell oocyte complexes (COCs group), and the denuded oocytes co-cultured with granulose cells (NkO-cc group). The un-vitrified fresh oocytes were used as the control group in experiments.

The in-vitro maturation was achieved by culturing in maturation medium supplemented with LH and FSH. Then the matured oocytes were subjected to in-vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). The capabilities of embryo development of the test oocytes were compared.

Statistical results showed that the NKO-cc group produced higher cleavage rate and blastocyst production than the COCs group, which also suggested a higher embryonic development in NKO-cc group. And there was no significant difference between the control group and the NKO-cc group. Blastocysts were generated by both IVF and ISCI, Whilst IVF resulted in better blastocyst development.

Reference

Summary 2

Reference

The List of recent publications in 'in vitro fertilization'

<pubmed limit=10>In Vitro Fertilization</pubmed>