ANAT2341 Lab 9: Difference between revisions

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==Organogenesis Lab==
==1. QUIZ==


== 1. QUIZ ==
==2. Guest Lecturer - Dr. Vashe Chandrakanthan==  
{|
| width=190px|[[File:Vashe_Chandrakanthan_profile_photo.jpg|180px]]


== 2. Organogenesis Lab ==
Dr. Vashe Chandrakanthan
In this lab you will dissect fertile chicken eggs and study fixed mouse embryos up to mid-gestation using dissection microscopes. You will name the embryonic anatomical structures, and describe what these will give rise to.
| '''Cardiac and Vascular Development and Haematopoiesis'''


[[Media:ANAT2341_-_INTRODUCTION_TO_THE_CHICK_EMBRYO.pdf|Organogensis Lab Manual]]


[[File:Chicken_Embryo_Hamburger_stages.jpg|600px|link=Hamburger Hamilton Stages]]
Dr Vashe Chandrakanthan earned his PhD in Medicine from University of Sydney and completed his postdoctoral studies at the Victor Chang Cardiac Research Institute in Sydney.  


''These are the Hamburger stages of chicken development''
Currently he is a Research Fellow at the Lowy Cancer Research Centre at the UNSW Australia. He is a member of the adult cancer program where he heads the mesenchymal stem cell biology team. He joined the stem cell program to help setting up a regenerative medicine program at the UNSW Australia.


Dr Chandrakanthan’s primary research interest lies in the field of developmental and molecular biology of stem cells. During his tenure at the Lowy Cancer Research Centre, in addition to setting up the regenerative medicine program, he has lineage traced mesenchymal stem cells and evaluated their contribution to various tissues in the mouse embryo with a particular focus on their role in haematopoiesis.


See also the [https://www.jove.com/video/306/windowing-chicken-eggs-for-developmental-studies JoVE article on chicken egg preparation]: <pubmed>18989413</pubmed>


[[Media:Chandrakanthan_V_Embryology_170831.pdf|'''Lecture Slides''']]
|}




More about chicken embryogenesis: [[Chicken Development]] | [[Hamburger Hamilton Stages]]


===References===
Search PubMed: [https://www.ncbi.nlm.nih.gov/pubmed/?term=Chandrakanthan%20V%5BAuthor%5D&cauthor=true&cauthor_uid=27044077 Chandrakanthan V]


<gallery>
File:HHstage1-4.jpg|stage 1-4
File:HHstage5-10.jpg|stages 5-10
File:HHstage11-14.jpg|stages 11-14
File:HHstage15-18.jpg|stages 15-18
File:HHstage19-21.jpg|stages 19-21
File:HHstage22-25.jpg|stages 22-25
File:HHstage26-28.jpg|stages 26-28
File:HHstage29-32.jpg|stage 29-32
</gallery>


[[File:Mouse_vs_Human_embryogenesis.jpg]]
{{#pmid:27044077}}


''This figure compares the human and mouse developmental stages''
{{#pmid:26830229}}


More about Mouse embryogenesis: [[Mouse Timeline Detailed]]
{{#pmid:27055868}}


{{#pmid:25870111}}


{{Chicken}}
===External Links===
{{External Links}}
* JOVE - [http://www.jove.com/science-education/5153/an-introduction-to-the-chick-gallus-gallus-domesticus An Introduction to the Chicken]




{{2018ANAT2341}}
{{2018ANAT2341}}

Revision as of 13:31, 23 August 2018

1. QUIZ

2. Guest Lecturer - Dr. Vashe Chandrakanthan

Vashe Chandrakanthan profile photo.jpg

Dr. Vashe Chandrakanthan

Cardiac and Vascular Development and Haematopoiesis


Dr Vashe Chandrakanthan earned his PhD in Medicine from University of Sydney and completed his postdoctoral studies at the Victor Chang Cardiac Research Institute in Sydney.

Currently he is a Research Fellow at the Lowy Cancer Research Centre at the UNSW Australia. He is a member of the adult cancer program where he heads the mesenchymal stem cell biology team. He joined the stem cell program to help setting up a regenerative medicine program at the UNSW Australia.

Dr Chandrakanthan’s primary research interest lies in the field of developmental and molecular biology of stem cells. During his tenure at the Lowy Cancer Research Centre, in addition to setting up the regenerative medicine program, he has lineage traced mesenchymal stem cells and evaluated their contribution to various tissues in the mouse embryo with a particular focus on their role in haematopoiesis.


Lecture Slides


References

Search PubMed: Chandrakanthan V


Chandrakanthan V, Yeola A, Kwan JC, Oliver RA, Qiao Q, Kang YC, Zarzour P, Beck D, Boelen L, Unnikrishnan A, Villanueva JE, Nunez AC, Knezevic K, Palu C, Nasrallah R, Carnell M, Macmillan A, Whan R, Yu Y, Hardy P, Grey ST, Gladbach A, Delerue F, Ittner L, Mobbs R, Walkley CR, Purton LE, Ward RL, Wong JW, Hesson LB, Walsh W & Pimanda JE. (2016). PDGF-AB and 5-Azacytidine induce conversion of somatic cells into tissue-regenerative multipotent stem cells. Proc. Natl. Acad. Sci. U.S.A. , 113, E2306-15. PMID: 27044077 DOI.

Thoms JA, Knezevic K, Liu JJ, Glaros EN, Thai T, Qiao Q, Campbell H, Packham D, Huang Y, Papathanasiou P, Tunningley R, Whittle B, Yeung AW, Chandrakanthan V, Hesson L, Chen V, Wong JW, Purton LE, Ward RL, Thomas SR & Pimanda JE. (2016). Arrested Hematopoiesis and Vascular Relaxation Defects in Mice with a Mutation in Dhfr. Mol. Cell. Biol. , 36, 1222-36. PMID: 26830229 DOI.

Huang Y, Thoms JA, Tursky ML, Knezevic K, Beck D, Chandrakanthan V, Suryani S, Olivier J, Boulton A, Glaros EN, Thomas SR, Lock RB, MacKenzie KL, Bushweller JH, Wong JW & Pimanda JE. (2016). MAPK/ERK2 phosphorylates ERG at serine 283 in leukemic cells and promotes stem cell signatures and cell proliferation. Leukemia , 30, 1552-61. PMID: 27055868 DOI.

Marks-Bluth J, Khanna A, Chandrakanthan V, Thoms J, Bee T, Eich C, Kang YC, Knezevic K, Qiao Q, Fitch S, Oxburgh L, Ottersbach K, Dzierzak E, de Bruijn MF & Pimanda JE. (2015). SMAD1 and SMAD5 Expression Is Coordinately Regulated by FLI1 and GATA2 during Endothelial Development. Mol. Cell. Biol. , 35, 2165-72. PMID: 25870111 DOI.


 2018 ANAT2341 - Timetable | Course Outline | Moodle | Tutorial 1 | Tutorial 2 | Tutorial 3

Labs: 1 Preimplantation and Implantation | 2 Reproductive Technology Revolution | 3 Group Projects | 4 GM manipulation mouse embryos | 5 Early chicken eggs | 6 Female reproductive tract | 7 Skin regeneration | 8 Vertebral development | 9 Organogenesis Lab | 10 Cardiac development | 11 Group projects | 12 Stem Cell Journal Club

Lectures: 1 Introduction | 2 Fertilization | 3 Week 1/2 | 4 Week 3 | 5 Ectoderm | 6 Placenta | 7 Mesoderm | 8 Endoderm | 9 Research Technology | 10 Cardiovascular | 11 Respiratory | 12 Neural crest | 13 Head | 14 Musculoskeletal | 15 Limb | 16 Renal | 17 Genital | 18 Endocrine | 19 Sensory | 20 Fetal | 21 Integumentary | 22 Birth | 23 Stem cells | 24 Revision

 Student Projects: Group Projects Information Project 1 | Project 3 | Project 4 | Project 5 | 2018 Test Student | Copyright