ANAT2341 Lab 6: Difference between revisions
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| [[File:Polycystic ovary syndrome mouse model.jpg|600px]] | | [[File:Polycystic ovary syndrome mouse model.jpg|600px]] | ||
| Experimental design | | '''Experimental design''' | ||
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For this study, PCOS was induced in wild-type, global, neuron-specific, and granulosa cell-specific androgen receptor knockout mice by s.c. inserting dihydrotestosterone implants in the mice for 3 mo. | |||
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Control mice were implanted with blank implants. | Control mice were implanted with blank implants. | ||
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Body weight, estrous cycling, blood pressure, fasting glucose, oral glucose tolerance, and insulin tolerance were assessed before collection of serum and tissues at 16 wk of age. | |||
[[File:Dihydrotestosterone.jpg|thumb|150px|link=Genital - Male Development|alt=Dihydrotestosterone structure cartoon|link=Genital - Male Development|Dihydrotestosterone]] | |||
'''Dihydrotestosterone''' - The hormonally active form of testosterone (male sex hormone) produced by enzyme (5-alpha reductase) conversion. In the male embryo, this can occur in the genital skin which then supports external genital development. In the adult, this conversion occurs in a number of different tissues. A known treatment for prostate cancer include 5-alpha reductase inhibitors. | |||
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Revision as of 15:19, 19 July 2018
1. QUIZ
2. Guest Lecturer - Dr Kirsty Walters
An Introduction to Polycystic Ovary Syndrome (PCOS)
<html5media width="600" height="400">https://www.youtube.com/embed/NhyYZCBq5A8</html5media>
Polycystic Ovary Syndrome Mouse Model
Caldwell ASL, Edwards MC, Desai R, Jimenez M, Gilchrist RB, Handelsman DJ & Walters KA. (2017). Neuroendocrine androgen action is a key extraovarian mediator in the development of polycystic ovary syndrome. Proc. Natl. Acad. Sci. U.S.A. , 114, E3334-E3343. PMID: 28320971 DOI.
References
Walters KA, Edwards MC, Tesic D, Caldwell ASL, Jimenez M, Smith JT & Handelsman DJ. (2018). The Role of Central Androgen Receptor Actions in Regulating the Hypothalamic-Pituitary-Ovarian Axis. Neuroendocrinology , 106, 389-400. PMID: 29635226 DOI.
Leloup-Hatey J, Baloche S & Jolivet-Jaudet G. (1988). [Changes in corticosterone and aldosterone concentrations in various tissues of Xenopus laevis tadpoles during the metamorphosis]. C. R. Seances Soc. Biol. Fil. , 182, 354-60. PMID: 2977958 1
Bertoldo MJ, Walters KA, Ledger WL, Gilchrist RB, Mermillod P & Locatelli Y. (2018). In-vitro regulation of primordial follicle activation: challenges for fertility preservation strategies. Reprod. Biomed. Online , , . PMID: 29503209 DOI.
Upton DH, Walters KA, McTavish KJ, Holt J, Handelsman DJ & Allan CM. (2018). Reproductive failure in mice expressing transgenic follicle-stimulating hormone is not caused by loss of oocyte quality. Biol. Reprod. , 98, 491-500. PMID: 29365049 DOI.
Walters KA & Handelsman DJ. (2017). Role of androgens in the ovary. Mol. Cell. Endocrinol. , , . PMID: 28687450 DOI.
Search PubMed: Walters KA