ANAT2341 Lab 6: Difference between revisions

Revision as of 14:58, 12 December 2016

Transgenic mouse models of human conditions

Transgenic Animal Unit (TAU)

Dr Delerue’s career spans over twenty years practice in animal handling, surgery, and generation of animal models of diseases. His research focuses on the production and characterization of new transgenic mouse models of human genetic disorders, using cutting-edge genome engineering techniques such as CRISPR/Cas9. UNSW Research Gateway: [1]

Recent articles

Delerue F & Ittner LM. Genome Editing in Mice Using CRISPR/Cas9: Achievements and Prospects Cloning and Transgenesis 2015, 4:135 doi:10.4172/2168-9849.1000135 [2]

Animal models are a powerful tool to understand the mechanisms underlying physiological and pathological processes in vivo. To date, mice remain the species most commonly used for genetic manipulation. The recent development of engineered endonucleases such as Zinc Finger Nucleases (ZFN), Transcription activator-like effector nucleases (TALEN), and the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) offered easy, flexible, and fast alternative to ES-Cell based gene targeting. Thanks to multiple advantages, the CRISPR system superseded its predecessors and became a popular method for genome editing. Here, we review the latest techniques to apply CRISPR editing to the mouse genome, and emphasize on the current methods used in transgenic laboratories and subsequent achievements in mice.

Ke YD, van Hummel A, Stevens CH, Gladbach A, Ippati S, Bi M, Lee WS, Krüger S, van der Hoven J, Volkerling A, Bongers A, Halliday G, Haass NK, Kiernan M, Delerue F, Ittner LM. Short-term suppression of A315T mutant human TDP-43 expression improves functional deficits in a novel inducible transgenic mouse model of FTLD-TDP and ALS. Acta Neuropathologica 2015 Nov;130(5):661-78 PubMed 26437864 [3]

Ittner A, Chua SW, Bertz J, Volkerling A, van der Hoven J, Gladbach A, Przybyla M, Bi M, van Hummel A, Stevens CH, Ippati S, Suh LS, Macmillan A, Sutherland G, Kril JJ, Silva APG, Mackay J, Poljak A, Delerue F, Ke YD, Ittner LM. Site-specific phosphorylation of tau inhibits amyloid-β toxicity in Alzheimer’s mice Science 2016 Nov;354 (6314):904-908 doi: 10.1126/science.aah6205 [4]

Recommended literature

Manipulating the Mouse Embryo: A Laboratory Manual (Fourth Edition) [5] By Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, & Andras Nagy.

Reproductive Engineering techniques in mice: Technical manual [6] Prof Naomi Nakagata, Center for Animal Resources and Development, Kumamoto University, Japan

@@ Line 1: / Line 1: @@
-{{ANAT2341Lab6}}
+== '''Transgenic mouse models of human conditions''' ==
-==Head Development==
-{|
-| [[File:Face animation.gif]]
-[[Face Development Movie]] | [[Media:Face_001.mp4|MP4 version]]
-| '''Development of the Face'''
+== '''Transgenic Animal Unit (TAU)''' ==
-This animation shows a ventral view of development of the human face from approximately week 5 through to neonate.
-The separate embryonic components that contribute to the face have been colour coded.
+Dr Delerue’s career spans over twenty years practice in animal handling, surgery, and generation of animal models of diseases. His research focuses on the production and characterization of new transgenic mouse models of human genetic disorders, using cutting-edge genome engineering techniques such as CRISPR/Cas9.
+UNSW Research Gateway: [https://research.unsw.edu.au/people/dr-fabien-delerue]
-* '''Frontonasal Prominence central portion (white)'''
-* <font color=blueviolet>'''Frontonasal Prominence - Lateral nasal'''</font> (purple)
-* <font color=yellowgreen>'''Frontonasal Prominence - Medial nasal'''</font> (green)
-* <font color=gold>'''Pharyngeal Arch 1 - Maxillary prominence'''</font> (yellow)
-* <font color=darkorange>'''Pharyngeal Arch 1 - Mandibular prominence'''</font> (orange)
-* '''Stomodeum (black)'''
-The five elements are: 1 Frontonasal Prominence, 2 Maxillary prominences and 2 Mandibular prominences.
+== '''Recent articles''' ==
-The [[S#stomodeum|stomodeum]] is the primordial mouth region and a surface central depression lying between the forebrain bulge and the heart bulge. At the floor of the stomodeum indentation is the [[B#buccopharyngeal membrane|buccopharyngeal membrane]] (oral membrane).
+Delerue F & Ittner LM. Genome Editing in Mice Using CRISPR/Cas9: Achievements and Prospects
+Cloning and Transgenesis 2015, 4:135 doi:10.4172/2168-9849.1000135 [http://www.omicsgroup.org/journals/genome-editing-in-mice-using-crisprcas9-achievements-and-prospects-2168-9849-1000135.php?aid=50992]
-Note the complex origin of the maxillary region (upper jaw) requiring the fusion of several embryonic elements, abnormalities of this process lead to [[C#cleft lip|cleft lip]] and [[C#cleft palate|cleft palate]].
+Animal models are a powerful tool to understand the mechanisms underlying physiological and pathological processes in vivo. To date, mice remain the species most commonly used for genetic manipulation. The recent development of engineered endonucleases such as Zinc Finger Nucleases (ZFN), Transcription activator-like effector nucleases  (TALEN), and the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) offered easy, flexible, and fast alternative to ES-Cell based gene targeting. Thanks to multiple advantages, the CRISPR system superseded its predecessors and became a popular method for genome editing. Here, we review the latest techniques to apply CRISPR editing to the mouse genome, and emphasize on the current methods used in transgenic laboratories and subsequent achievements in mice.
-|}
+Ke YD, van Hummel A, Stevens CH, Gladbach A, Ippati S, Bi M, Lee WS, Krüger S, van der Hoven J, Volkerling A, Bongers A, Halliday G, Haass NK, Kiernan M, Delerue F, Ittner LM. Short-term suppression of A315T mutant human TDP-43 expression improves functional deficits in a novel inducible transgenic mouse model of FTLD-TDP and ALS. Acta Neuropathologica 2015 Nov;130(5):661-78 PubMed 26437864 [https://dx.doi.org/10.1007/s00401-015-1486-0]
-==Aim==
+Ittner A, Chua SW, Bertz J, Volkerling A, van der Hoven J, Gladbach A, Przybyla M, Bi M, van Hummel A, Stevens CH, Ippati S, Suh LS, Macmillan A, Sutherland G, Kril JJ, Silva APG, Mackay J, Poljak A, Delerue F,  Ke YD, Ittner LM. Site-specific phosphorylation of tau inhibits amyloid-β toxicity in Alzheimer’s mice
-{|
+Science 2016 Nov;354 (6314):904-908 doi: 10.1126/science.aah6205 [http://science.sciencemag.org/content/354/6314/904.full]
-| To introduce the developmental embryology of the head and face, and their associated abnormalities.
-The class will include an introduction to some of the early sensory system development (placode and pharyngeal arches). Also covered in a later lecture.
-| {{OneMinuteClock}}
-[[One_Minute_Embryology#Human_Face_and_Palate|'''1 Minute Embryology''']]<br>[https://thebox.unsw.edu.au/video/1-minute-embryology-face-and-palate UNSW theBox]
-|}
-==Specific Objectives==
-# To understand the formation and contribution of the pharyngeal arches to face and neck development.
-# To know the main structures derived from components of the pharyngeal arches (groove, pouch and arch connective tissue).
-# To briefly understand some abnormalities associated with head development.
-==Textbooks==
-{|
-|-
-| {{Embryo logocitation}}
-|
-{{Head Links}}
-{{Neural Crest Links}}
-[[Sensory System Development]]
-|-
-| [[File:The Developing Human, 10th edn.jpg|90px]]
-| {{MPT2015APAcitation}} (links only function with UNSW connection)
-Chapter 9 [http://www.unsw.eblib.com.wwwproxy0.library.unsw.edu.au/patron/Read.aspx?p=2074364&pg=216 Pharyngeal Apparatus, Face, and Neck]
-Chapter 18 [http://www.unsw.eblib.com.wwwproxy0.library.unsw.edu.au/patron/Read.aspx?p=2074364&pg=553 Development of Eyes and Ears]
-{{UNSW textbook - The Developing Human}}
-|-
-| [[File:Larsen's human embryology 5th ed.jpg|90px]]
-| {{Larsen2015APAcitation}}(links only function with UNSW connection)
-Chapter 17 [http://www.unsw.eblib.com.wwwproxy0.library.unsw.edu.au/patron/Read.aspx?p=2074524&pg=447 Development of the Pharyngeal Apparatus and Face]
-Chapter 18 [http://www.unsw.eblib.com.wwwproxy0.library.unsw.edu.au/patron/Read.aspx?p=2074524&pg=491 Development of the Ears]
-{{UNSW textbook - Larsen's Human Embryology}}
-|-
-| Other Online Respiratory Resources
-|
-|}
-{{ANAT2341Lab6}}
-==Movies==
-===Head and Face===
-{|
-| valign="bottom"|{{Face movie}}
-| valign="bottom"|{{Human Stage16-18 face}}
-| valign="bottom"|{{Human_Stage15-22_head}}
-| valign="bottom"|{{Palate 1}}
-| valign="bottom"|{{Palate 2}}
-| valign="bottom"|{{Tongue movie}}
-|-
-| valign="bottom"|{{Fetal week 10 palate movie}}
-| valign="bottom"|{{Mandible movie}}
-| valign="bottom"|{{Mouse cranial neural crest movie}}
-| valign="bottom"|{{Mouse Face Bmp4 movie}}
-| valign="bottom"|{{Mouse face microCT movie}}
-| valign="bottom"|{{Mouse cephalic plexus movie}}
-|}
-===Adult Skull===
-{{Adult skull movies}}
-===Neural Crest===
-{{Chicken neural crest movies}}
-{{ANAT2341Lab6}}
+== '''Recommended literature''' ==
+Manipulating the Mouse Embryo: A Laboratory Manual (Fourth Edition) [http://cshlpress.com/default.tpl?cart=147944968860031853&action=full&--eqskudatarq=982]
+By Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, & Andras Nagy.
+Reproductive Engineering techniques in mice: Technical manual [http://card.medic.kumamoto-u.ac.jp/card/english/sigen/manual/onlinemanual.html]
+Prof Naomi Nakagata, Center for Animal Resources and Development, Kumamoto University, Japan
 {{2016ANAT2341 footer}}