ANAT2341 Lab 10: Difference between revisions

Revision as of 08:49, 8 October 2018

Organogenesis Lab

1. QUIZ

2. Organogenesis Lab

In this lab you will dissect fertile chicken eggs and study fixed mouse embryos up to mid-gestation using dissection microscopes. You will name the embryonic anatomical structures, and describe what these will give rise to.

Organogensis Lab Manual

These are the Hamburger stages of chicken development

See also the JoVE article on chicken egg preparation: <pubmed>18989413</pubmed>

Additional Chicken Links

Viktor Hamburger (1900 – 2001)

More about chicken embryogenesis: Chicken Development | Hamburger Hamilton Stages

Historic Chicken Embryology
1883 History of the Chick \| 1900 Chicken Embryo Development Plates \| 1904 X-Ray Effects \| 1910 Somites \| 1914 Primordial Germ Cells 1919 Lillie Textbook \| 1920 Chick Early Embryology \| 1933 Neural \| 1939 Sternum \| 1948 Limb \| Movie 1961 \| Historic Papers

stage 1-4
stages 5-10
stages 11-14
stages 15-18
stages 19-21
stages 22-25
stages 26-28
stage 29-32

This figure compares the human and mouse developmental stages

More about Mouse embryogenesis: Mouse Timeline Detailed

chicken

External Links

External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name. Links to any external commercial sites are provided for information purposes only and should never be considered an endorsement. UNSW Embryology is provided as an educational resource with no clinical information or commercial affiliation.

JOVE - An Introduction to the Chicken

@@ Line 1: / Line 1: @@
-==Skin Development and Regeneration==
+==Organogenesis Lab==
+== 1. QUIZ ==
+== 2. Organogenesis Lab ==
+In this lab you will dissect fertile chicken eggs and study fixed mouse embryos up to mid-gestation using dissection microscopes. You will name the embryonic anatomical structures, and describe what these will give rise to.
+[[Media:Fertile_Egg_practical_Class_2.pdf|Organogensis Lab Manual]]
+[[File:Chicken_Embryo_Hamburger_stages.jpg|600px|link=Hamburger Hamilton Stages]]
-== 1. QUIZ ==
+''These are the Hamburger stages of chicken development''
+See also the [https://www.jove.com/video/306/windowing-chicken-eggs-for-developmental-studies JoVE article on chicken egg preparation]: <pubmed>18989413</pubmed>
+===Additional Chicken Links===
+[[File:Viktor Hamburger.jpg|thumb|alt=Viktor Hamburger|link=Embryology History - Viktor Hamburger|Viktor Hamburger (1900 – 2001)]]
+More about chicken embryogenesis: [[Chicken Development]] | [[Hamburger Hamilton Stages]]
+<br>
+{{Chicken links}}
+<br>
+<gallery>
+File:HHstage1-4.jpg|stage 1-4
+File:HHstage5-10.jpg|stages 5-10
+File:HHstage11-14.jpg|stages 11-14
+File:HHstage15-18.jpg|stages 15-18
+File:HHstage19-21.jpg|stages 19-21
+File:HHstage22-25.jpg|stages 22-25
+File:HHstage26-28.jpg|stages 26-28
+File:HHstage29-32.jpg|stage 29-32
+</gallery>
+[[File:Mouse_vs_Human_embryogenesis.jpg]]
+''This figure compares the human and mouse developmental stages''
-== 2. Dr. Annemiek Beverdam (UNSW) - "Stem cell regulation in normal skin regeneration and in skin disease" ==
+More about Mouse embryogenesis: [[Mouse Timeline Detailed]]
-[[File:Annemiek Beverdam profile photo.jpeg]]
-Dr. Annemiek Beverdam studies the genetic processes that govern development, homeostasis and regeneration of the skin in the mouse. Her research aims at understanding the genetic and molecular basis of developmental and human regenerative skin diseases such as skin cancer, which affects 2 out of 3 Australians in their life time. Her lab recently made the pivotal discoveries that Yes-associated protein (YAP) functions acts as a key molecular switch in epidermal stem/progenitor cell proliferation and differentiation [https://www.ncbi.nlm.nih.gov/pubmed/23190885] by driving β-Catenin Activation [https://www.ncbi.nlm.nih.gov/pubmed/27816394]. Dr. Beverdam currently investigates the developmental genetic context in which YAP functions to control skin stem/progenitor cells in normal and in disrupted skin biology. She employs genetically manipulated mouse models, human skin samples, advanced imaging technology such as confocal microscopy and whole mouse in vivo imaging, gene and protein expression analyses and whole genome approaches to address her research questions. Her research will open up exciting new avenues for translational research and the development of treatments for human regenerative skin disease such as skin cancer and eczema.
+{{Chicken}}
-'''References:'''
+===External Links===
-Akladios B., Mendoza-Reinoso V., Samuel MS.,  Hardeman E., Khosrotehrani K., Key B; Beverdam A (2016). ‘Epidermal YAP2-5SA-ΔC Drives β-Catenin Activation to Promote Keratinocyte Proliferation in the Mouse Skin in vivo’ [https://www.ncbi.nlm.nih.gov/pubmed/27816394].
+{{External Links}}
-Liang H*, Akladios B*, Canales CP*, Francis R, Hardeman EH, Beverdam A. (2016) CUBIC protocol visualizes protein expression at single cell resolution in whole mount skin preparations (2016). JoVE [https://www.ncbi.nlm.nih.gov/pubmed/27584943].
+* JOVE - [http://www.jove.com/science-education/5153/an-introduction-to-the-chick-gallus-gallus-domesticus An Introduction to the Chicken]
-Corley SM, Canales C, Carmona-Mora P, Mendoza-Reinoso V, Beverdam A, Hardeman EH, Wilkins M, Palmer S. (2016). RNA-Seq analysis of Gtf2ird1 knockout epidermal tissue provides potential insights into molecular mechanisms underpinning Williams-Beuren syndrome. BMC Genomics [https://www.ncbi.nlm.nih.gov/pubmed/27295951].
-Beverdam A., Claxton C., Zhang., X., James G., Harvey KF, Key B. (2013). YAP controls stem/progenitor cell proliferation in the mouse postnatal epidermis. Journal of Investigative Dermatology [https://www.ncbi.nlm.nih.gov/pubmed/23190885]
+{{2018ANAT2341}}