ANAT2241 This practical support page content is not part of the virtual science practical class and provides additional information for student self-directed learning purposes. All practical class pages are located on Moodle - ANAT2241
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Course Description
Practicals
Cross-Discipline
Some of the practical support pages may contain additional links to related topics in other disciplines in the biomedical sciences. These are listed towards the bottom of each page and are provided to allow potential integration of the histology topic with other disciplines. These are not directly examinable.
Course Links
Moodle - ANAT2241 - 2019
- Histology Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | ANAT2241 Support | Histology | Histology Stains | Embryology Glossary
Common Histology Stains
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Histology Stains - Common Stains and Their Reactions
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Stain
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Nucleus
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Cytoplasm
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Collagen
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RBCs
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Other
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Haematoxylin
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blue
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-
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-
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-
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mucins - light blue
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Eosin
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-
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pink
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pale pink
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bright red
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colloid - pinkmuscle - red
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Iron Haematoxylin
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blue/black
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-
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-
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-
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Van Gieson
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-
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brown/yellow
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red
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yellow
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muscle: yellow/browncartilage - pink
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Verhoeff's Elastin
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black
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-
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-
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-
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elastic fibres - black
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Tartrazine
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-
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yellow
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yellow
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yellow
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Silver Impregnation
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-
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-
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grey/brown
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-
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reticular fibres - black
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Methyl Green
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dark green
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light green
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light green
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green
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Nuclear Fast Red
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red
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pink
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pink
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pink
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Gomori's Trichrome
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purple/red
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purple
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green
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red
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keratin - redmuscle - purple/red
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Heidenhain's Azan
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red
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purple/red
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deep blue
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red
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muscle - red
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Osmium Tetroxide
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-
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-
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brown
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brown
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myelin, lipids - black
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Alcian Blue
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-
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-
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-
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-
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mucins, - blue
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Periodic acid-Schiff (PAS)
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-
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-
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pink
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-
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mucins, glycogen, glycocalyx - magenta
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Phosphotungstic Acid-Hematoxylin (PTAH)
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blue
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-
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red
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blue
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muscle bands - blue
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Masson's Trichrome
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blue/black
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red
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green/blue
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red
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cartilage, mucins - blue or green; muscle - red
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Luxol Fast Blue
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-
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-
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-
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variable
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myelin - blue
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Aldehyde Fuchsin
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-
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-
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-
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-
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elastic fibres, mast cells - deep purple
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Light Green
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-
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-
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light green
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-
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Gallocyanin
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dark blue
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-
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-
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-
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nucleic acids, Nissl granules - dark blue
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Romanowsky (e.g. Leishman's)
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blue
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pink
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acidophils - red, basophils - blue, azurophilic - purple
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Aldehyde Pararosanilin
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elastic fibres - purple
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Haematoxylin and Eosin
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One of the most common staining techniques in pathology and histology. Acronym "H and E" stain. (H&E, HE).
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Haematoxylin
- Stains nuclei blue to dark-blue.
- Stains the matrix of hyaline cartilage, myxomatous, and mucoid material pale blue.
- Stains myelin weakly but is not noticeable if combined with eosin stain.
- combined with Orange G (H & Or. G.) instead of eosin, specifically stains the granules of acidophilic cells of the adenohypophysis (anterior pituitary).
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Eosin
- Stains cytoplasm pink to red; red blood cells are also bright red.
- Common counterstain to hematoxylin.
- Stain intensity varies with the formula as well as the fixative.
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Practical Support
- Pages can be accessed from any internet connected computer.
ANAT2241 Support Links: The Virtual Microscope | Covering and Lining Epithelia | Glandular Epithelia | CT Components | CT Types | Bone, Bone Formation and Joints | Muscle | Nervous | Blood | Eye | Cardiovascular | Respiratory | Integumentary | Gastrointestinal | Gastrointestinal Organs | Lymphatic and Immune | Endocrine | Urinary | Female Reproductive | Male Reproductive | Histology Stains | Histology Drawings | Practicals Health and Safety 2013 | Moodle - 2019
ANAT2241 This practical support page content is not part of the science practical class and provides only background information for student self-directed learning purposes.
Cite this page: Hill, M.A. (2024, June 11) Embryology ANAT2241 Histology - Basic and Systematic. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/ANAT2241_Histology_-_Basic_and_Systematic
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- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G