ANAT2241 Histology - Basic and Systematic

From Embryology

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ANAT2241 This practical support page content is not part of the virtual science practical class and provides additional information for student self-directed learning purposes. All practical class pages are located on Moodle - 2019 ANAT2241

Course Description

Patrick de Permentier
(Course coordinator)

The aim of this course is to provide students with a thorough understanding of the microscopic appearance and function of normal structures in the human body. This allows students to integrate this information with other disciplines such as Gross Anatomy, Pathology, and Physiology.

  • The Basic Histology component of the course will concentrate on the microanatomy of the four basic tissues, namely: epithelial tissue, including glandular tissue, connective tissue, muscular tissue, and nervous tissue.
  • The Systematic Histology component of the course will investigate how these basic tissues combine to form organs, which operate together to maintain homeostasis.


Please note - the 2019 course manual is an online version only, linked each week through the Moodle home page.

Practicals

ANAT2241 practical room

Practicals Health and Safety 2019


ANAT2241 Practical and Support Links (2019)
Week VSlides Support
Week 1 Epithelia and Glands Glands Epithelia
Week 2 Connective Tissue CT Types CT Components
Week 3 Bone, Joints and Muscle Bone and Joints Muscle
Week 4 Nervous Tissue Nervous
Week 5 Blood (online) and Eye Mid-Semester Assessment
Week 6 Cardiovascular and Respiratory Cardiovascular Respiratory
Week 7 Integumentary and Immune System Integumentary Lymphatic and Immune
Week 8 Gastro-Intestinal System GIT 1 GIT 2
Week 9 Endocrine and Urinary Endocrine Urinary
Week 10 Female and Male Reproductive Female Male

General Histology Information

Anatomy is the study of the structure of organs and tissues at the MACROSCOPIC (or gross) level. Histology is the study of organ and tissue structure at the MICROSCOPIC level - it can be considered as microanatomy. Histology provides an insight into how cellular components are structurally and functionally related. It draws its foundations in Biochemistry, Molecular Biology and Physiology as well as Gross Anatomy. Histology provides valuable information on why tissues and organs are shaped as they are. Histology is one of the bases of biomedical sciences. Modern histological techniques allow us to explore and gain understanding of biochemical and physiological processes and how these are changed when structure is changed, as occurs, for instance, in many disease processes. By the end of this course, students should have a thorough understanding of the tissues and systems of the body by microscopic examination and to apply their knowledge to functional states examined in Physiology and diseased states examined in Pathology.

Textbooks

Several books provide adequate coverage of the material in this course. A number of suggestions have been included on the following list. An atlas on its own will usually only cover the practical part of the course, so you will need access to a textbook to cover the theory part of the course.

Combined Texts and Atlas

  • Young, B., Lowe, J., Stevens, A. & Heath, J. (2006) Wheater’s Functional Histology. A Text and Colour Atlas 5th ed. Churchill Livingstone, Edinburgh. ISBN 0-443-06850X. UNSW Online
  • Junqueira L. (2009) Basic Histology Text & Atlas 12th ed, McGraw-Hill, ISBN 9780071630207

Textbook

  • Kerr, J. B. (2010) Functional Histology 2nd ed. Mosby Elsevier. ISBN 9780729538374.

Atlas

  • Eroschenko, V.P. (2008) di Fiore’s Atlas of Histology. 11th ed Lippincott, Williams & Wilkins Int., Baltimore. ISBN 9781608314928.

Histology Drawings

Images on Practical Support Pages

  • The support class pages contain both labelled and unlabelled histology images.
  • These images are generally not from the virtual slide set.
    • You should use these images to help understand the unlabelled, and examinable, virtual slides used in your practical classes.

Opening Images Separately

  • Histology images on support pages can be opened separately as larger images with more information.
    • To open the image, simply click on the image.
    • Use your browser option to "open image in new tab".
  • Additional information on image page may not always relate directly to histology.
  • To see how this image relates to other topics, look at the bottom of the image information page "File usage".

Cross-Discipline

Some of the practical support pages may contain additional links to related topics in other disciplines in the biomedical sciences. These are listed towards the bottom of each page and are provided to allow potential integration of the histology topic with other disciplines. These are not examinable.

This is also shown when images are opened separately, at the bottom of the image information page is a heading "File usage". This indicates related histology, embryology and anatomy pages that use this image. These pages can be opened by clicking their title in "File usage" section.



Course Links

Moodle - ANAT2241 - 2019

Histology Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | ANAT2241 Support | Histology | Histology Stains | Embryology Glossary


Common Histology Stains  
Histology Stains - Common Stains and Their Reactions
Stain
Nucleus
Cytoplasm
Collagen
RBCs
Other
Haematoxylin
blue
-
-
-
mucins - light blue
Eosin
-
pink
pale pink
bright red
colloid - pinkmuscle - red
Iron Haematoxylin
blue/black
-
-
-
Van Gieson
-
brown/yellow
red
yellow
muscle: yellow/browncartilage - pink
Verhoeff's Elastin
black
-
-
-
elastic fibres - black
Tartrazine
-
yellow
yellow
yellow
Silver Impregnation
-
-
grey/brown
-
reticular fibres - black
Methyl Green
dark green
light green
light green
green
Nuclear Fast Red
red
pink
pink
pink
Gomori's Trichrome
purple/red
purple
green
red
keratin - redmuscle - purple/red
Heidenhain's Azan
red
purple/red
deep blue
red
muscle - red
Osmium Tetroxide
-
-
brown
brown
myelin, lipids - black
Alcian Blue
-
-
-
-
mucins, - blue
Periodic acid-Schiff (PAS)
-
-
pink
-
mucins, glycogen, glycocalyx - magenta
Phosphotungstic Acid-Hematoxylin (PTAH)
blue
-
red
blue
muscle bands - blue
Masson's Trichrome
blue/black
red
green/blue
red
cartilage, mucins - blue or green; muscle - red
Luxol Fast Blue
-
-
-
variable
myelin - blue
Aldehyde Fuchsin
-
-
-
-
elastic fibres, mast cells - deep purple
Light Green
-
-
light green
-
Gallocyanin
dark blue
-
-
-
nucleic acids, Nissl granules - dark blue
Romanowsky (e.g. Leishman's)
blue
pink
acidophils - red, basophils - blue, azurophilic - purple
Aldehyde Pararosanilin elastic fibres - purple
Haematoxylin and Eosin
One of the most common staining techniques in pathology and histology. Acronym "H and E" stain. (H&E, HE).


Haematoxylin
  • Stains nuclei blue to dark-blue.
  • Stains the matrix of hyaline cartilage, myxomatous, and mucoid material pale blue.
  • Stains myelin weakly but is not noticeable if combined with eosin stain.
  • combined with Orange G (H & Or. G.) instead of eosin, specifically stains the granules of acidophilic cells of the adenohypophysis (anterior pituitary).
Eosin
  • Stains cytoplasm pink to red; red blood cells are also bright red.
  • Common counterstain to hematoxylin.
  • Stain intensity varies with the formula as well as the fixative.

Practical Support

Pages can be accessed from any internet connected computer.

ANAT2241 Support Links: The Virtual Microscope | Covering and Lining Epithelia | Glandular Epithelia | CT Components | CT Types | Bone, Bone Formation and Joints | Muscle | Nervous | Blood | Eye | Cardiovascular | Respiratory | Integumentary | Gastrointestinal | Gastrointestinal Organs | Lymphatic and Immune | Endocrine | Urinary | Female Reproductive | Male Reproductive | Histology Stains | Histology Drawings | Practicals Health and Safety 2013 | Moodle - 2019


ANAT2241 This practical support page content is not part of the science practical class and provides only background information for student self-directed learning purposes.


Cite this page: Hill, M.A. (2019, September 24) Embryology ANAT2241 Histology - Basic and Systematic. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/ANAT2241_Histology_-_Basic_and_Systematic

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© Dr Mark Hill 2019, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G