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'''Assessment 1:''' Find two recent research references on fertilisation or in vitro fertilisation and write a summary of the research article method and findings. | '''Assessment 1:''' Find two recent research references on fertilisation or in vitro fertilisation and write a summary of the research article method and findings. | ||
PMID | PMID 26285703 '''Does obesity have detrimental effects on IVF treatment outcomes?''' <ref><pubmed>26285703</pubmed></ref> | ||
This study looked at the influence of BMI on IVF treatment outcomes, particularly those undergoing ICSI treatments. | |||
'''Method:''' | '''Method:''' | ||
Participants underwent IVF following standard procedures and embryos were transferred into the uterus at either 3 or 5 days. On day 12 of the ET, patients had B-hCG levels assessed and once exceeded 2000IU/L they underwent transvaginal ultrasounds to confirm pregnancy. A number of key parameters of the patient and the IVF-ICSI were considered and analyzed; including patient age, antral follicle count and BMI and number of retrieved oocytes, proportion of oocytes fertilized and total gonadotropin dose, respectively. Patients were then divided into three groups based on their BMI value; normal, overweight and obese. Those classified as underweight were excluded due to the small number of patients. Finally, potential correlations between BMI, total gonadotropin use and other parameters and success of IVF-ICSI treatments were evaluated. | |||
'''Results:''' | '''Results:''' | ||
It was found that there was a significant difference in total gonadotropin dose and stimulation duration across all weight categories. Specifically, it was demonstrated that both gonadotropin dose and stimulation duration were higher in participants classified as obese. Other findings included that rates of clinical pregnancy, spontaneous abortion and ongoing pregnancies were approximately equal across all three weight groups. | |||
Revision as of 21:56, 3 September 2015
ANAT2341 Course Work
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- 2015 Course: Week 2 Lecture 1 Lecture 2 Lab 1 | Week 3 Lecture 3 Lecture 4 Lab 2 | Week 4 Lecture 5 Lecture 6 Lab 3 | Week 5 Lecture 7 Lecture 8 Lab 4 | Week 6 Lecture 9 Lecture 10 Lab 5 | Week 7 Lecture 11 Lecture 12 Lab 6 | Week 8 Lecture 13 Lecture 14 Lab 7 | Week 9 Lecture 15 Lecture 16 Lab 8 | Week 10 Lecture 17 Lecture 18 Lab 9 | Week 11 Lecture 19 Lecture 20 Lab 10 | Week 12 Lecture 21 Lecture 22 Lab 11 | Week 13 Lecture 23 Lecture 24 Lab 12 | 2015 Projects: Three Person Embryos | Ovarian Hyper-stimulation Syndrome | Polycystic Ovarian Syndrome | Male Infertility | Oncofertility | Preimplantation Genetic Diagnosis | Students | Student Designed Quiz Questions | Moodle page
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Lab 1
Assessment 1: Find two recent research references on fertilisation or in vitro fertilisation and write a summary of the research article method and findings.
PMID 26285703 Does obesity have detrimental effects on IVF treatment outcomes? [1]
This study looked at the influence of BMI on IVF treatment outcomes, particularly those undergoing ICSI treatments.
Method:
Participants underwent IVF following standard procedures and embryos were transferred into the uterus at either 3 or 5 days. On day 12 of the ET, patients had B-hCG levels assessed and once exceeded 2000IU/L they underwent transvaginal ultrasounds to confirm pregnancy. A number of key parameters of the patient and the IVF-ICSI were considered and analyzed; including patient age, antral follicle count and BMI and number of retrieved oocytes, proportion of oocytes fertilized and total gonadotropin dose, respectively. Patients were then divided into three groups based on their BMI value; normal, overweight and obese. Those classified as underweight were excluded due to the small number of patients. Finally, potential correlations between BMI, total gonadotropin use and other parameters and success of IVF-ICSI treatments were evaluated.
Results:
It was found that there was a significant difference in total gonadotropin dose and stimulation duration across all weight categories. Specifically, it was demonstrated that both gonadotropin dose and stimulation duration were higher in participants classified as obese. Other findings included that rates of clinical pregnancy, spontaneous abortion and ongoing pregnancies were approximately equal across all three weight groups.
PMID 26264981 Aging-related premature luteinization of granulosa cells is avoided by early oocyte retrieval. [2]
This study compared the grnaulosa cell function across three different age groups; young oocyte donors (21-29 y.o.a) middle aged oocyte donors (30-37 y.o.a) and older infertile patients (43-47 y.o.a).
Methods:
Total Number of Patients: 136
Group 1: ages 21-29; 31 patients
Group 2: ages 30-37; 64 patients
Group 3: ages 43-47; 41 patients
All subjects underwent controlled hyperstimulation and oocyte maturation, followed by a transvaginal ultrasound-guided oocyte retrieval. Oocyte donors were stimulated in a long gonadotropin releasing hormone agonist cycle and infertile patients were stimulated in microdose agonist cycles. Oocytes collected at retrievals were cultured and those classified as mature (presence of 1 polar body) were fertilised and further cultured in Blastocyst medium for three days. Real time PCR and western blot in the granulosa cells collected from follicular fluid were used to analyse the relationship between gene expression and gonadotropin activity, steriodogenesis, apoptosis and luteinization.
Results:
It was demonstrated by a down regulation of "FSH receptor (FSHR), aromatase (CYP19A1) and 17β-hydroxysteroid dehydrogenase (HSD17B) expression" and an up regulation of "LH receptor (LHCGR), P450scc (CYP11A1) and progesterone receptor (PGR)" with increasing age of patients that age related functional decline in granulosa cells were consistent with premature luteinization. Patients who received earlier retrieval to avoid premature luteinization demonstrated a marginal increase in oocyte prematurity, however, exhibited improved embryo numbers and quality as well as satisfactory clinical pregnancy rates.
From these results, the researches concluded that earlier retrieval of oocytes can be utilised to avoid premature follicular luteinzation, which is a key contributor to the rapidly declining successful IVF results in women over 43.
Lab 2 Images
Uploading Images in 5 Easy Steps | ||
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First Read the help page Images and Copyright Tutorial.
Students cannot delete images once uploaded. You will need to email me with the full image name and request deletion, that I am happy to do with no penalty if done before I assess. Non-Table version of this page
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Syrian Hamster In Vitro Fertilisation PMID- 24852961[3]
Lab 3
Research articles associated with male infertility in humans.
1. Aydos SE, Karadağ A, Özkan T, Altınok B, Bunsuz M, Heidargholizadeh S, Aydos K, Sunguroğlu A. Association of MDR1 C3435T and C1236T single nucleotide polymorphisms with male factor infertility. PMID 26125837
2. V. A. Giagulli, Carbone, G. De Pergola, E. Guastamacchia, F. Resta, B. Licchelli, C. Sabbà, and V. Triggiani Could androgen receptor gene CAG tract polymorphism affect spermatogenesis in men with idiopathic infertility? PMID 24691874
3. Lazaros L, Xita N, Takenaka A, Sofikitis N, Makrydimas G, Stefos T, Kosmas I, Zikopoulos K, Hatzi E, Georgiou I. Semen quality is influenced by androgen receptor and aromatase gene synergism. PMID 23001776
References
PMID 26244658
look at this[4]
Here's the list