Musculoskeletal System - Bone Development
|Embryology - 25 May 2017 Expand to Translate|
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- 1 Introduction
- 2 Some Recent Findings
- 3 Adult Human Skeleton
- 4 Textbooks
- 5 Objectives
- 6 Development Overview
- 7 Ossification Centres
- 8 Endochondral Ossification
- 9 Intramembranous Ossification
- 10 Bone Structure
- 11 Bone Cells
- 12 Bone Matrix
- 13 Haversian Systems
- 14 Molecular
- 15 Abnormalities
- 16 References
- 17 Additional Images
- 18 Terms
- 19 External Links
- 20 Glossary Links
The mesoderm forms nearly all the connective tissues of the musculoskeletal system, except within the head where neural crest also contributes connective tissues. Each tissue (cartilage, bone, and muscle) goes through many different mechanisms of differentiation.
The 2 key developmental processes are the initial "patterning" of bone location and then the overt "differentiation" of bone through the process of ossification. For details on specific bone differentiation in human development see Bone Development Timeline.
Bone is formed through a lengthy process involving ossification of a cartilage formed from mesenchyme. Two main forms of ossification occur in different bones, intramembranous (eg skull) and endochondrial (eg limb long bones) ossification. Ossification continues postnatally, through puberty until mid 20s. Early ossification occurs at the ends of long bones.
The two major parts of the human skeleton are the axial (80 bones in skull, vertebra, ribs, sternum) and appendicular (126 bones in limbs, shoulders, pelvis) skeletons.
Musculoskeletal and limb abnormalities are one of the largest groups of congenital abnormalities.
Some Recent Findings
|More recent papers|
This table shows an automated computer PubMed search using the listed sub-heading term.
References listed on the rest of the content page and the associated discussion page (listed under the publication year sub-headings) do include some editorial selection based upon both relevance and availability.
Massara Mzid, Riadh Badraoui, Sameh Ben Khedir, Zouheir Sahnoun, Tarek Rebai Protective effect of ethanolic extract of Urtica urens L. against the toxicity of imidacloprid on bone remodeling in rats and antioxidant activities. Biomed. Pharmacother.: 2017, 91;1022-1041 PubMed 28531918
Gül Merve Yalcin-Ulker, Alev Cumbul, Gonca Duygu-Capar, Ünal Uslu, Kemal Sencift Preventive Effect of Phosphodiesterase Inhibitor Pentoxifylline Against Medication-Related Osteonecrosis of the Jaw: An Animal Study. J. Oral Maxillofac. Surg.: 2017; PubMed 28529150
Kaija Schaepe, Janina Werner, Kristina Glenske, Tessa Bartges, Anja Henss, Marcus Rohnke, Sabine Wenisch, Jürgen Janek ToF-SIMS study of differentiation of human bone-derived stromal cells: new insights into osteoporosis. Anal Bioanal Chem: 2017; PubMed 28516281
Iris Ribitsch, Souyet Chang-Rodriguez, Monika Egerbacher, Simone Gabner, Sinan Gueltekin, Johann Huber, Therese Schuster, Florien Jenner Sheep Placenta Cotyledons: A Noninvasive Source of Ovine Mesenchymal Stem Cells. Tissue Eng Part C Methods: 2017, 23(5);298-310 PubMed 28504918
Valerie D Roobrouck, Esther Wolfs, Michel Delforge, Dorien Broekaert, Soumen Chakraborty, Kathleen Sels, Thomas Vanwelden, Bryan Holvoet, Larissa Lhoest, Satish Khurana, Shubham Pandey, Chloé Hoornaert, Peter Ponsaerts, Tom Struys, Nancy Boeckx, Peter Vandenberghe, Christophe M Deroose, Catherine M Verfaillie Multipotent adult progenitor cells improve the hematopoietic function in myelodysplasia. Cytotherapy: 2017, 19(6);744-755 PubMed 28499585
Adult Human Skeleton
- The Developing Human: Clinically Oriented Embryology (8th Edition) by Keith L. Moore and T.V.N Persaud - Moore & Persaud Chapter 15 the skeletal system
- Larsen’s Human Embryology by GC. Schoenwolf, SB. Bleyl, PR. Brauer and PH. Francis-West - Chapter 11 Limb Dev (bone not well covered in this textbook)
- Before we Are Born (5th ed.) Moore and Persaud Chapter 16,17: p379-397, 399-405
- Essentials of Human Embryology Larson Chapter 11 p207-228
- Identify the components of a somite and the adult derivatives of each component.
- Give examples of sites of (a) endochondral and (b) intramembranous ossification and to compare these two processes.
- Identify the general times (a) of formation of primary and (b) of formation of secondary ossification centres, and (c) of fusion of such centres with each other.
- Briefly summarise the development of the limbs.
- Describe the developmental abnormalities responsible for the following malformations: selected growth plate disorders; congenital dislocation of the hip; scoliosis; arthrogryposis; and limb reduction deformities.
Below is a very brief overview using simple figures of 3 aspects of early musculoskeletal development. More detailed overviews are shown on other notes pages Mesoderm and Somite, Vertebral Column, Limb in combination with serial sections and Carnegie images.
|Cells migrate through the primitive streak to form mesodermal layer. Extraembryonic mesoderm lies adjacent to the trilaminar embryo totally enclosing the amnion, yolk sac and forming the connecting stalk.||Paraxial mesoderm accumulates under the neural plate with thinner mesoderm laterally. This forms 2 thickened streaks running the length of the embryonic disc along the rostrocaudal axis. In humans, during the 3rd week, this mesoderm begins to segment. The neural plate folds to form a neural groove and folds.||Segmentation of the paraxial mesoderm into somites continues caudally at 1 somite/90minutes and a cavity (intraembryonic coelom) forms in the lateral plate mesoderm separating somatic and splanchnic mesoderm. Note intraembryonic coelomic cavity communicates with extraembryonic coelom through portals (holes) initially on lateral margin of embryonic disc.||Somites continue to form. The neural groove fuses dorsally to form a tube at the level of the 4th somite and "zips up cranially and caudally and the neural crest migrates into the mesoderm.|
- Primary ossification centres are the first sites of bone formation and where cartilage has begun to degenerate. In long bones, this lis generally located mid-diaphysis (shaft). In other bones (e.g. base of skull) these are the initial locations of bone formation.
- Secondary ossification centres develop in the cartilage epiphysis of the long bones.
- No medulary cavity forms in a secondary ossification center.
- Appears late in fetal development.
- Used as a marker for term development if a secondary ossification centre present in either: head of femur, head of tibia, of head of humerus.
- The last secondary centre to appear is the clavical medial epiphysis, that does not develop until 18 or 20 years.
Most of the bony skeleton forms by this process, that replaces a developmental cartilage template with bone.
|Longitudinal views of endochondral bone formation in mouse limbs.|
|a - Prx1+ progenitors from lateral plate mesoderm proliferate to populate the emerging limb bud.||b - Cells nearest the centre undergo mesenchymal condensation, express Col2a1 as they enter a chondrogenic differentiation program, and deposit a cartilage template.||c to d - Differentiating cells upregulate Col10a1 as they become hypertrophic, which triggers local formation of a bone collar and vascularization of the cartilage template. Invading blood vessels deliver an influx of haematopoietic cells that give rise to osteoclasts which excavate the cartilage template, and Osx1+ osteoblast progenitors and other blood cell types that populate the newly formed marrow cavity.||d - A longitudinal growth axis is established when vascularization and osteoclast-mediated resorption bisect the presumptive skeletal element, producing two growth plates with opposing directionality. A perpendicular growth axis is driven by periosteal osteoblasts and allows the bone to grow in width.||e - Within the remodelled cartilage template, bone-forming osteoblasts are derived from Osx1+ cells arriving with the invading vasculature, as well as hypertrophic Col10a1+ chondroctyes that transdifferentiate as they exit the growth plate into the marrow cavity. As bones grow in length and width, a second wave of vascularization forms the secondary ossification centres.||f - Mature endochondral bone.|
See also Bone Histology
- Links: Blue Histology - endochondral | Dev Biology - endochondral ossification | endochondral ossification animation
Only specific parts of the skeleton form by this process, where bone forms by direct ossification of mesenchyme without a pre-existing cartilage template. This process occurs in regions of the skull and the clavicle. See also this recent review.
See also Bone Histology
Human Fetal Head (12 week)
- (dense) no spaces or hollows in the bone matrix visible to the eye.
- forms the thick-walled tube of the shaft (or diaphysis) of long bones, which surrounds the marrow cavity (or medullary cavity). A thin layer of compact bone also covers the epiphyses of long bones.
- (cancellous or spongy bone) consists of delicate bars (spicules) and sheets of bone, trabeculae
- branch and intersect to form a sponge-like network
- ends of long bones (or epiphyses) consist mainly of trabecular bone.
Connective tissue covering the surface of bone (except articular surfaces). The embryonic origin of this layer is still controversial.
Connective tissue lining inner surface of bone.
- Appositional growth occurs at either the periosteum (outer surface), or the endosteum (inner surface).
- Osteoblasts secrete osteoid, a pre-bone material composed mainly of type I collagen that becomes mineralized.
- Early bone matrix deposited in development and during repair is woven rather than lamellar in appearance and structure.
- In development, there are 2 distinct types of bone formation (intramembranous and endochondral)
- derive from osteogenic stem cells the osteoprogenitor cells that differentiate to form pre-osteoblast then osteoblasts maturing to an osteocyte
- osteoprogenitor cells - "resting cell" line the inner and outer surfaces of bone
- mature bone-forming cells embedded in lacunae within the bone matrix
- osteoblasts and osteocytes - secrete organic matrix of bone (osteoid), converted into osteocytes when become embedded in matrix (which calcifies soon after deposition)
- bone-resorbing multinucleated macrophage-like cells
- origin- fusion of monocytes or macrophages, Blood macrophage precursor, Attach to bone matrix
- seal a small segment of extracellular space (between plasma membrane and bone surface), HCl and lysosomes secreted into this space by osteoclasts dissolves calcium phosphate crystals (give bone rigidity and strength)
- Resorptive bay - (Howship's lacuna) shallow bay lying directly under an osteoclast.
- do not mistake for megakaryocytes, found in bone marrow not associated with bone matrix.
- megakaryocytes are also multi-niucleated and form platelets
- red marrow - mainly haematopoietic (myeloid) tissue, newborn has all red marrow
- yellow marrow - mainly fat cells, found in diaphysis region of long bones
- stromal cells - all other support cells not involved in haematopoiesis
- Links: Blood Development
Marrow stroma components:
- osteoblasts - enclose the marrow compartment in bone tissue.
- endothelial and smooth muscle cells - organized into a complex vascular network composed of arterioles, capillaries, sinusoids, and a large central vein.
- nerves - sensory and sympathetic nerve fibres, glia, and perineural cells that innervate the marrow compartment to form a neural network.
- adipocytes - support metabolic functions of the bone marrow.
- stromal cells - support haematopoiesis and retain skeletal potential.
The bone matrix has 2 major components.
- Organic portion composed of mainly collagen Type 1 (about 95%) and amorphous ground substance.
- Inorganic portion (50% dry weight of the matrix) composed of hydroxyapatite crystals, calcium, phosphorus, bicarbonate, nitrate, Mg, K, Na.
- storage calcium and phosphate
- regulate blood calcium levels
- also called osteons
- Volkmann's canals - interconnect Haversian systems
- concentric - surrounding each Haversian System
- interstitial - bony plates that fill in between the haversian systems.
- circumferential - layers of bone that underlie the periosteum and endosteum
- osteocytes extending cytoplasmic processes into canaliculi
- Additional Histology images: low | medium | high
The transcription factors Runx2 and Runx3 are essential for chondrocyte maturation, while Runx2 and Osterix are essential for osteoblast differentiation.
Osterix (OSX) encodes a transcription factor containing three Cys2-His2 zinc-finger DNA-binding domains at its C terminus that has been shown to be essential for bone formation.
Osteogenesis Imperfecta (OI, brittle bone disease) originally described as a collagen 1 gene mutation, but can have several different genetic causes and can be classified into eight different types (I-VIII).
- COL1A1 and COL1A2 mutations
- CRTAP and LEPRE1 mutations, in severe/lethal and recessively inherited osteogenesis imperfecta
- Urs H Langen, Mara E Pitulescu, Jung Mo Kim, Rocio Enriquez-Gasca, Kishor K Sivaraj, Anjali P Kusumbe, Amit Singh, Jacopo Di Russo, M Gabriele Bixel, Bin Zhou, Lydia Sorokin, Juan M Vaquerizas, Ralf H Adams Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. Nat. Cell Biol.: 2017, 19(3);189-201 PubMed 28218908
- Yuh-Charn Lin, Steve R Roffler, Yu-Ting Yan, Ruey-Bing Yang Disruption of Scube2 impairs endochondral bone formation. J. Bone Miner. Res.: 2015; PubMed 25639508
- Sonja M Walzer, Erdal Cetin, Ruth Grübl-Barabas, Irene Sulzbacher, Beate Rueger, Werner Girsch, Stefan Toegel, Reinhard Windhager, Michael B Fischer Vascularization of primary and secondary ossification centres in the human growth plate. BMC Dev. Biol.: 2014, 14;36 PubMed 25164565 | BMC Dev Biol.
- Antonella Galli, Dimitri Robay, Marco Osterwalder, Xiaozhong Bao, Jean-Denis Bénazet, Muhammad Tariq, Renato Paro, Susan Mackem, Rolf Zeller Distinct roles of Hand2 in initiating polarity and posterior Shh expression during the onset of mouse limb bud development. PLoS Genet.: 2010, 6(4);e1000901 PubMed 20386744 | PMC2851570 | PLoS Genet.
- Valerie S Salazar, Laura W Gamer, Vicki Rosen BMP signalling in skeletal development, disease and repair. Nat Rev Endocrinol: 2016; PubMed 26893264
- Christopher J Percival, Joan T Richtsmeier Angiogenesis and intramembranous osteogenesis. Dev. Dyn.: 2013, 242(8);909-22 PubMed 23737393
- Wen'an Xu, Qiuyue Chen, Cuixian Liu, Jiajing Chen, Fu Xiong, Buling Wu A novel, complex RUNX2 gene mutation causes cleidocranial dysplasia. BMC Med. Genet.: 2017, 18(1);13 PubMed 28173761
- Jay R Shapiro, Paul D Sponsellor Osteogenesis imperfecta: questions and answers. Curr. Opin. Pediatr.: 2009, 21(6);709-16 PubMed 19907330
Masaharu Takigawa CCN2: a master regulator of the genesis of bone and cartilage. J Cell Commun Signal: 2013, 7(3);191-201 PubMed 23794334
Yingzi Yang Skeletal morphogenesis during embryonic development. Crit. Rev. Eukaryot. Gene Expr.: 2009, 19(3);197-218 PubMed 19883365
E J Mackie, Y A Ahmed, L Tatarczuch, K-S Chen, M Mirams Endochondral ossification: how cartilage is converted into bone in the developing skeleton. Int. J. Biochem. Cell Biol.: 2008, 40(1);46-62 PubMed 17659995
- BONE is an interdisciplinary forum for the rapid publication of original articles and reviews on basic, translational, and clinical aspects of bone and mineral metabolism.
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- e-radiography Ossification
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Cite this page: Hill, M.A. 2017 Embryology Musculoskeletal System - Bone Development. Retrieved May 25, 2017, from https://embryology.med.unsw.edu.au/embryology/index.php/Musculoskeletal_System_-_Bone_Development
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