Embryology History - John Gurdon
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Sir John Gurdon (born 1933) in 1962 used nuclear transplantation and cloning to show that the nucleus of a differentiated somatic cell retains the totipotency necessary to form a whole organism.
|Frog Links: Frog Development | 2009 Student Project | Hans Spemann | Wilhelm Roux | 1921 Early Frog Development | 1951 Rana pipiens Development | Rana pipiens Images | Frog Glossary | John Gurdon | Category:Frog | Animal Development|
Some Recent Findings
The Nobel Prize in Physiology or Medicine 2012 was awarded jointly to Sir John B. Gurdon and Shinya Yamanaka
- 2012 - The Nobel Prize in Physiology or Medicine 2012 awarded jointly with Shinya Yamanaka "for the discovery that mature cells can be reprogrammed to become pluripotent".
Methods of transplanting nuclei from single cultured cells to unfertilized frogs' eggs
- "Two methods of transplanting single nuclei from monolayers of cultured cells to unfertilized eggs of Xenopus laevis are described, illustrated, and tested.
The detached-cell method is simpler and quicker to operate and is suitable for homogeneous populations of cells which are easily removed from the substrate on which they are growing. The other, attached-cell, method is technically more elaborate, but is applicable to cells whose properties can be individually determined under the phase-contrast microscope and to cells which are not readily dissociated from other cells or from their substrate."
Genetic content of adult somatic cells tested by nuclear transplantation from cultured cells
- "It has yet to be proved that somatic cells of an adult animal possess genes other than those necessary for their own growth and differentiation. A particularly stringent test of the genetic content of the nucleus of a somatic cell is provided by transplanting it to an enucleated egg. The development of the egg tests the capacity of the genes in the transplanted nucleus to elicit normal early development and differentiation. Although this method has been applied to certain cells of embryos, larvae, and newly metamorphosed froglets, it has not been possible, until now, to transfer nuclei successfully from the cells of normal adult animals."
- John Gurdon The birth of cloning: an interview with John Gurdon. [Interview by Kristin Kain]. Dis Model Mech: 2009, 2(1-2);9-10 PubMed 19132124
Patrick Narbonne, David E Simpson, John B Gurdon Deficient induction response in a Xenopus nucleocytoplasmic hybrid. PLoS Biol.: 2011, 9(11);e1001197 PubMed 22131902
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- Kei Miyamoto, Vincent Pasque, Jerome Jullien, John B Gurdon Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes. Genes Dev.: 2011, 25(9);946-58 PubMed 21536734
- J B Gurdon, R A Laskey Methods of transplanting nuclei from single cultured cells to unfertilized frogs' eggs. J Embryol Exp Morphol: 1970, 24(2);249-55 PubMed 4923666 | Development | PDF
- R A Laskey, J B Gurdon Genetic content of adult somatic cells tested by nuclear transplantation from cultured cells. Nature: 1970, 228(5278);1332-4 PubMed 5530658 | PDF
J B Gurdon, J A Byrne The first half-century of nuclear transplantation. Biosci. Rep.: 2005, 24(4-5);545-57 PubMed 16134025
J B Gurdon The use of Xenopus oocytes and embryos as a route towards cell replacement. J. Biosci.: 2005, 30(1);11-4 PubMed 15824436
J Gurdon Not a total waste of time. An interview with John Gurdon. Interview by James C Smith. Int. J. Dev. Biol.: 2000, 44(1);93-9 PubMed 10761853
J B Gurdon Embryonic induction--molecular prospects. Development: 1987, 99(3);285-306 PubMed 3308408
J B Gurdon, R A Laskey The transplantation of nuclei from single cultured cells into enucleate frogs' eggs. J Embryol Exp Morphol: 1970, 24(2);227-48 PubMed 5531072
J B Gurdon, V Uehlinger "Fertile" intestine nuclei. Nature: 1966, 210(5042);1240-1 PubMed 5967799
J B GURDON Adult frogs derived from the nuclei of single somatic cells. Dev. Biol.: 1962, 4;256-73 PubMed 13903027
T R ELSDALE, J B GURDON, M FISCHBERG A description of the technique for nuclear transplantation in Xenopus laevis. J Embryol Exp Morphol: 1960, 8;437-44 PubMed 13726553
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- Wellcome Trust/Cancer Research UK Gurdon Institute is part of Cambridge University with funding from the listed two main sponsors, and other sources, supports research into the complementary areas of Cancer and Developmental Biology.
- John Gurdon - Research Lab
- Berkeley interview March 16, 2006 | YouTube
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Cite this page: Hill, M.A. 2017 Embryology Embryology History - John Gurdon. Retrieved October 24, 2017, from https://embryology.med.unsw.edu.au/embryology/index.php/Embryology_History_-_John_Gurdon
- © Dr Mark Hill 2017, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G