User:Z3463890

Lab Attendance

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--Z3463890 (talk) 12:51, 14 August 2015 (AEST)

Lab Assessment 1

Article 1

"Effect of vitamin D status on clinical pregnancy rates following in vitro fertilization" ^[1] PMID 25077107

Summary

According to this study, vitamin D may play a role in human reproduction. Therefore, the aim of this study was to find out whether there is a correlation between vitamin D levels and implantation and clinical pregnancy rates in infertile women following IVF.

Method

• Total of 173 infertile women participated in the study that met the following criteria: aged 18-41 years, follicle stimulating hormone level 12 IU/L or lower and able to provide informed consent.

• 25(OH)D samples were collected within 1 week before oocyte retrieval from those infertile women.

• Vitamin D status was evaluated and determined by serum 25-hydroxy-vitamin D (25[OH]D) levels.

• Patients were classified in two different groups; having sufficient (≥ 75 nmol/L) or insufficient (or deficient; hereafter referred to as “insufficient”; < 75 nmol/L) serum levels of 25(OH)D.

• Patient demographics and IVF cycle parameters between two groups were compared.

• Clinical pregnancy, as identified by ultrasound following 4-5 weeks after embryo transfer; was the primary outcome measurement.

Findings

According to the outcome of this study, the women with sufficient levels of 25(OH)D had significantly higher rates of clinical pregnancy (52.5%) per IVF cycle started than that with insufficient levels (34.7%). Therefore, Vitamin D supplementation can potentially provide an easy and cost-effective way of improving pregnancy rates but requires further investigations as the results are not statistically significant in the sufficient 25(OH)D group.

Article 2

"Examining the temperature of embryo culture in in vitro fertilization: a randomized controlled trial comparing traditional core temperature (37°C) to a more physiologic, cooler temperature (36°C)." ^[2] PMID 25044079

The aim of this study was to illustrate the better clinical outcome of blastulation and pregnancy rates in human clinical IVF in a more physiologically cooler temperature i.e. 36°C, compare to the traditional core temperature of 37 degrees Celsius.

Method

• 52 Infertile couples with a female partner less than 42 years old were selected for this study.

• 8 or more oocytes from a female of 42 years of age, with infertile couples (n=52) were retrieved.

• Mature oocytes obtained from a single cohort of oocytes were randomly divided into two groups. One group was cultured at 37°C and the other at 36°C. These conditions kept as it is from the time of intracytoplasmic sperm injection (ICSI) until the time of verification (embryo transfer).

• Paired embryo transfers were done by transferring an euploid embryo from both group.

• DNA fingerprinting was used to determine the outcome for each embryo.

It is important to note that, some factors were measured throughout the study to measure the main outcomes and highlight which of these conditions clinically improved the embryonic development. These factors are: rate of development of expanded blastocysts, fertilization, aneuploidy, and sustained implantation.

Findings

According to this investigation, paired analysis shows a slightly higher usable rate of blastocyst formation per zygote at the 37°C environment (48.4%), compare to the other group at the 36°C culture (41.2%). Rates of fertilization, aneuploidy, and sustained implantation were equivalent. In conclusion, IVF culture at 36 degrees does not improve the conditions for blastulation and pregnancy rates in human in IVF. Thus, keeping the traditional temperature or decreasing it to 36 degrees does not have any advantages to embryo development .

References

↑ <pubmed>25077107</pubmed>
↑ <pubmed>25044079</pubmed>

Lab 2 - Images

Uploading Images in 5 Easy Steps

First Read the help page Images and Copyright Tutorial.

Hint - This exercise is best done by using separate tabs on your browser so that you can keep all the relevant pages easily available. You can also use your own discussion page to copy and paste links, text. PMIDs etc that you will need in this process.

Find an image .
1. Search PubMed using an appropriate search term. Note that there is a special library of complete (full online) article and review texts called PubMed Central (PMC). Be very careful, while some of these PMC papers allow reuse, not all do and to add the reference link to your image you will still need to use the PMID.
2. You can also make your own search term. In this link example PMC is searched for images related to "embryo+implantation" http://www.ncbi.nlm.nih.gov/pmc/?term=embryo+implantation&report=imagesdocsum. simply replace "embryo+implantation" with your own search term, but remember not everything in PMC can be reused, you will still need to find the "copyright notice" on the full paper, no notice, no reuse.
3. Where else can I look? BioMed Central is a separate online database of journals that allow reuse of article content. Also look at the local page Journals that provides additional resources.
4. You have found an image, go to step 2.
Check the Copyright. I cannot emphasise enough the importance of this second step.
1. The rule is unless there is an obvious copyright statement that clearly allows reuse (there are several different kinds of copyright, some do not) located in the article or on the article page, move on and find another resource. Not complying with this is a serious academic infringement equivalent to plagiarism."Plagiarism at UNSW is defined as using the words or ideas of others and passing them off as your own." (extract from UNSW statement on Academic Honesty and Plagiarism)
2. You have found the statement and it allows reuse, go to step 3.
Downloading your image.
1. Download the image to your own computer. Either use the download image on the page or right click the image.
2. To find the downloaded image you may have to look in your computer downloads folder, or the default location for downloaded files.
3. The image file will have its own original name, that you will not be using on the wiki. You can rename it now (see renaming below), but you should also make a note of the original name.
4. Make sure you have everything ready then for the
5. You have the image file on your computer, go to step 4.
Uploading your image.
1. First make sure you have all the information you want to use with the file readily available. There is also a detailed description below.
2. Towards the bottom of the lefthand menuunder “Toolbox” click Upload file. This will open a new window.
3. In the top window "Source file", click "Choose file" and then navigate to find the file on the computer. and select the image.
4. If you have done this correctly the upload window will now have your image file shown in choose file and also in the lower window "File description" in "Destination filename:" DO NOT CLICK UPLOAD FILE YET.
5. Rename your file in "Destination filename:" this should be a brief filename that describes the image. Not any of the following - the original file name, image, file, my image, your ZID, etc. Many of the common embryology names may have already been used, but you can add a number (01, 02, 03, etc) or the PMID number to the filename to make it unique.
6. If the filename or image has already been used or exists it will be shown on the upload page. If another student has already uploaded that image you will have to find another file. Duplicated images will not receive a mark, so check before you upload as you cannot delete images.
7. In the "Summary" window for now just paste the PMID. You will come back and edit this information.
8. Now click "Upload image" at the bottom of the window, go to step 4.
Edit and Add to your page.
1. Edit - Open the image with the "Edit" tab at the top of its page. You should see the PMID you had pasted earlier in the new edit window. Add the following information to the summary box.
  1. Image Title as a sub-heading. Under this title add the original figure legend or your own description of the image.
  2. Image Reference sub-sub-heading. Use the PMID link method shown in Lab 1 and you can also have a direct link to the original Journal article.
  3. Image Copyright sub-sub-heading. Add the copyright information under this sub-sub-heading exactly as shown in the original paper.
  4. Student Image template, as shown here {{Template:Student Image}} to show that it is a student uploaded image.
2. Add - Now add your image to your own page under a subheading for Lab 2 Assessment including a description and a reference link. If still stuck with this last step, look at the example on the Test Student page.
3. Done!

Students cannot delete images once uploaded. You will need to email me with the full image name and request deletion, that I am happy to do with no penalty if done before I assess.

Non-Table version of this page

Stress Relief....
<html5media height="480" width="640">http://www.youtube.com/watch?v=i9Hwn2DOgKo</html5media>

Image showing Dynamic Localization of Two Membrane Proteins Required for Fertilization^[1]

↑ <pubmed>18050412</pubmed>

Template:TestStudent2015

Please do not use your real name on this website, use only your student number.

Glossary Links

Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link

[1] <pubmed>25077107</pubmed>

[2] <pubmed>25044079</pubmed>

[3] <pubmed>18050412</pubmed>

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[2]

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