User:Z3463890
Lab Attendance
--Z3463890 (talk) 13:46, 7 August 2015 (AEST) --Z3463890 (talk) 12:51, 14 August 2015 (AEST)
Lab Assessment 1
Article 1
"Effect of vitamin D status on clinical pregnancy rates following in vitro fertilization" [1] PMID 25077107
Summary
According to this study, vitamin D may play a role in human reproduction. Therefore, the aim of this study was to find out whether there is a correlation between vitamin D levels and implantation and clinical pregnancy rates in infertile women following IVF.
Method
• Total of 173 infertile women participated in the study that met the following criteria: aged 18-41 years, follicle stimulating hormone level 12 IU/L or lower and able to provide informed consent.
• 25(OH)D samples were collected within 1 week before oocyte retrieval from those infertile women.
• Vitamin D status was evaluated and determined by serum 25-hydroxy-vitamin D (25[OH]D) levels.
• Patients were classified in two different groups; having sufficient (≥ 75 nmol/L) or insufficient (or deficient; hereafter referred to as “insufficient”; < 75 nmol/L) serum levels of 25(OH)D.
• Patient demographics and IVF cycle parameters between two groups were compared.
• Clinical pregnancy, as identified by ultrasound following 4-5 weeks after embryo transfer; was the primary outcome measurement.
Findings
According to the outcome of this study, the women with sufficient levels of 25(OH)D had significantly higher rates of clinical pregnancy (52.5%) per IVF cycle started than that with insufficient levels (34.7%). Therefore, Vitamin D supplementation can potentially provide an easy and cost-effective way of improving pregnancy rates but requires further investigations as the results are not statistically significant in the sufficient 25(OH)D group.
Article 2
"Examining the temperature of embryo culture in in vitro fertilization: a randomized controlled trial comparing traditional core temperature (37°C) to a more physiologic, cooler temperature (36°C)." [2] PMID 25044079
The aim of this study was to illustrate the better clinical outcome of blastulation and pregnancy rates in human clinical IVF in a more physiologically cooler temperature i.e. 36°C, compare to the traditional core temperature of 37 degrees Celsius.
Method
• 52 Infertile couples with a female partner less than 42 years old were selected for this study.
• 8 or more oocytes from a female of 42 years of age, with infertile couples (n=52) were retrieved.
• Mature oocytes obtained from a single cohort of oocytes were randomly divided into two groups. One group was cultured at 37°C and the other at 36°C. These conditions kept as it is from the time of intracytoplasmic sperm injection (ICSI) until the time of verification (embryo transfer).
• Paired embryo transfers were done by transferring an euploid embryo from both group.
• DNA fingerprinting was used to determine the outcome for each embryo.
It is important to note that, some factors were measured throughout the study to measure the main outcomes and highlight which of these conditions clinically improved the embryonic development. These factors are: rate of development of expanded blastocysts, fertilization, aneuploidy, and sustained implantation.
Findings
According to this investigation, paired analysis shows a slightly higher usable rate of blastocyst formation per zygote at the 37°C environment (48.4%), compare to the other group at the 36°C culture (41.2%). Rates of fertilization, aneuploidy, and sustained implantation were equivalent. In conclusion, IVF culture at 36 degrees does not improve the conditions for blastulation and pregnancy rates in human in IVF. Thus, keeping the traditional temperature or decreasing it to 36 degrees does not have any advantages to embryo development .
References
References
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