User:Z3416054

Hi there Just giving the editor a good old test run. Test student 2015

Lab Attendance

Please do not use your real name on this website, use only your student number.

Glossary Links

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--Z3416054 (talk) 13:45, 7 August 2015 (AEST) --Mark Hill (talk) 10:47, 6 August 2015 (AEST) Thanks for setting up your page. We will be talking more about this in the Practical on Friday.

Picture Tutorial

Uploading Images in 5 Easy Steps

First Read the help page Images and Copyright Tutorial.

Hint - This exercise is best done by using separate tabs on your browser so that you can keep all the relevant pages easily available. You can also use your own discussion page to copy and paste links, text. PMIDs etc that you will need in this process.

Find an image .
1. Search PubMed using an appropriate search term. Note that there is a special library of complete (full online) article and review texts called PubMed Central (PMC). Be very careful, while some of these PMC papers allow reuse, not all do and to add the reference link to your image you will still need to use the PMID.
2. You can also make your own search term. In this link example PMC is searched for images related to "embryo+implantation" http://www.ncbi.nlm.nih.gov/pmc/?term=embryo+implantation&report=imagesdocsum. simply replace "embryo+implantation" with your own search term, but remember not everything in PMC can be reused, you will still need to find the "copyright notice" on the full paper, no notice, no reuse.
3. Where else can I look? BioMed Central is a separate online database of journals that allow reuse of article content. Also look at the local page Journals that provides additional resources.
4. You have found an image, go to step 2.
Check the Copyright. I cannot emphasise enough the importance of this second step.
1. The rule is unless there is an obvious copyright statement that clearly allows reuse (there are several different kinds of copyright, some do not) located in the article or on the article page, move on and find another resource. Not complying with this is a serious academic infringement equivalent to plagiarism."Plagiarism at UNSW is defined as using the words or ideas of others and passing them off as your own." (extract from UNSW statement on Academic Honesty and Plagiarism)
2. You have found the statement and it allows reuse, go to step 3.
Downloading your image.
1. Download the image to your own computer. Either use the download image on the page or right click the image.
2. To find the downloaded image you may have to look in your computer downloads folder, or the default location for downloaded files.
3. The image file will have its own original name, that you will not be using on the wiki. You can rename it now (see renaming below), but you should also make a note of the original name.
4. Make sure you have everything ready then for the
5. You have the image file on your computer, go to step 4.
Uploading your image.
1. First make sure you have all the information you want to use with the file readily available. There is also a detailed description below.
2. Towards the bottom of the lefthand menuunder “Toolbox” click Upload file. This will open a new window.
3. In the top window "Source file", click "Choose file" and then navigate to find the file on the computer. and select the image.
4. If you have done this correctly the upload window will now have your image file shown in choose file and also in the lower window "File description" in "Destination filename:" DO NOT CLICK UPLOAD FILE YET.
5. Rename your file in "Destination filename:" this should be a brief filename that describes the image. Not any of the following - the original file name, image, file, my image, your ZID, etc. Many of the common embryology names may have already been used, but you can add a number (01, 02, 03, etc) or the PMID number to the filename to make it unique.
6. If the filename or image has already been used or exists it will be shown on the upload page. If another student has already uploaded that image you will have to find another file. Duplicated images will not receive a mark, so check before you upload as you cannot delete images.
7. In the "Summary" window for now just paste the PMID. You will come back and edit this information.
8. Now click "Upload image" at the bottom of the window, go to step 4.
Edit and Add to your page.
1. Edit - Open the image with the "Edit" tab at the top of its page. You should see the PMID you had pasted earlier in the new edit window. Add the following information to the summary box.
  1. Image Title as a sub-heading. Under this title add the original figure legend or your own description of the image.
  2. Image Reference sub-sub-heading. Use the PMID link method shown in Lab 1 and you can also have a direct link to the original Journal article.
  3. Image Copyright sub-sub-heading. Add the copyright information under this sub-sub-heading exactly as shown in the original paper.
  4. Student Image template, as shown here {{Template:Student Image}} to show that it is a student uploaded image.
2. Add - Now add your image to your own page under a subheading for Lab 2 Assessment including a description and a reference link. If still stuck with this last step, look at the example on the Test Student page.
3. Done!

Students cannot delete images once uploaded. You will need to email me with the full image name and request deletion, that I am happy to do with no penalty if done before I assess.

Non-Table version of this page

Stress Relief....
<html5media height="480" width="640">http://www.youtube.com/watch?v=i9Hwn2DOgKo</html5media>

Lab 1 Assessment

Article 1

This research article aimed to determine the clinical outcomes following IVF (in vitro fertilisation) and embryo transfer treatments in subjects suffering from the sexually transmitted disease syphilis. The clinical outcomes mentioned are the rates of pregnancy and health of newborns following treatment via IVF. Couples engaged in sexual intercourse over 12 months without the use of contraception and who failed to conceive were deemed as being infertile.

The subjects were divided into two groups based on serology results, a syphilis infected group and a control group, each with 160 individuals, giving a total of 320 subjects. The Syphilis infected group was further divided into three subcategories, a male infected group, a female infected group and a couple (male and female) infected group. Penicillin G20 (an anti-syphilis treatment) was given to the individuals in the syphilis group. IVF treatment commenced one month proceeding the disappearance of clinical syphilis symptoms or if test results gave a negative result for syphilis infection.

The results of this experiment revealed no significant differences in regards to the basal FSH and LH of both the control and syphilis groups. However, the thickness of the endometrium differed greatly, with the syphilis group demonstrating a thicker endometrial wall (16.9±5.4mm) compared to the control group (13.0±4.7mm). Further differences were noted in blastocyst implantation rates, with the syphilis group having less successful implantations compared to the control (24.2% vs. 34.4% respectively).Normal oocyte cleavage differed between the two groups with the syphilis group demonstrating less normal oocyte cleavage compared to the control group (6.3±4.7 vs. 8.1±4.6). Furthermore, the clinical pregnancy rates of the syphilis group stood at 43.8% compared to 55.6% of the control group.

Syphilis infection appeared to have a significant impact on the success and clinical outcomes of IVF. Syphilis associated pelvic inflammatory disease can lead to an increase in the thickness of the endometrium, which can adversely affect blastocyte implantation and endometrial receptivity. Successful pregnancy rates typically correlate with an endometrial thickness of 7-14mm, with any thickness beyond 14mm often corresponding with decreased clinical pregnancies. Rates of clinical pregnancy and miscarriage rates did not differ between the three syphilis subgroups. Conception involving a male infected partner was associated with a shorter gestational period and decreased offspring birth weight, when compared to the female infected and couple infected subgroups. No explanation for this phenomenon was provided.

PMID 26208116

Article 1 Reference: <pubmed>PMC4514756</pubmed>

Article 2

The effects of oxygen levels on factors such as cleavage, implantation and pregnancy rates in IVF cultured embryos was the primary focus of this article. Women between the ages of 20-48 who were seeking treatment for infertility were utilised in this experiment. Gametes were allocated to be incubated in one of three environments, each with a different oxygen concentration. The first group was placed in an atmosphere with an oxygen concentration of 20%. The second group rested in a 20% oxygen environment for a day before being moved to a 5% oxygen, 5% carbon dioxide and 90% nitrogen atmosphere. The third group consisted of a 5% carbon dioxide, 5% oxygen and 90% nitrogen atmosphere. The gametes (spermatozoa and oocytes) were incubated together in their respective environmental conditions for 4-6 hours after which fertilisation is presumed to have occurred.

Successful embryos were transferred at Day 3 cleavage. A biochemical analysis of HCG validated pregnancy, whilst ultrasound was used to confirm the presence of a heartbeat 28 days following the transfer. IVF fertilisation rates were calculated as being the number of fertilised oocytes over the number of oocytes inseminated. Cleavage rates were characterised by the number of blastomeres over the number of fertilised and abortion rate by the amount of miscarriages divided by the number of transfers.

The research article concluded that the embryos from the 5% oxygen group had the highest rates of fertilisation and implantation. The 20% oxygen group had the second highest rates of fertilisation and maintained excellent embryo quality, whilst the 20% to 5% group had the lowest rates overall. Abortion and miscarriage rates did not differ at all between the three groups. The group incubated at 5% oxygen demonstrated higher quality embryos and increased rates of pregnancy when compared to the 20% oxygen group. The article concludes that implantation, pregnancy and embryo quality can be somewhat affected by a set oxygen concentration, but are affected adversely by a shift from one concentration to another. Shifting from one oxygen concentration to another appeared to have an adverse effect on the cleavage of the embryo and would likely impact future development and the overall success of the IVF treatment.

PMID 26131222

Article 2 Reference: <pubmed>PMC4483955</pubmed>

Lab 2 Assessment

References

PMID 26244658

Look at this!<ref><pubmed>26244658</pubmed>