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Common Histology Stains
Histology Stains - Common Stains and Their Reactions Stain Nucleus Cytoplasm Collagen RBCs Other Haematoxylin blue - - - mucins - light blue Eosin - pink pale pink bright red colloid - pinkmuscle - red Iron Haematoxylin blue/black - - - Van Gieson - brown/yellow red yellow muscle: yellow/browncartilage - pink Verhoeff's Elastin black - - - elastic fibres - black Tartrazine - yellow yellow yellow Silver Impregnation - - grey/brown - reticular fibres - black Methyl Green dark green light green light green green Nuclear Fast Red red pink pink pink Gomori's Trichrome purple/red purple green red keratin - redmuscle - purple/red Heidenhain's Azan red purple/red deep blue red muscle - red Osmium Tetroxide - - brown brown myelin, lipids - black Alcian Blue - - - - mucins, - blue Periodic acid-Schiff (PAS) - - pink - mucins, glycogen, glycocalyx - magenta Phosphotungstic Acid-Hematoxylin (PTAH) blue - red blue muscle bands - blue Masson's Trichrome blue/black red green/blue red cartilage, mucins - blue or green; muscle - red Luxol Fast Blue - - - variable myelin - blue Aldehyde Fuchsin - - - - elastic fibres, mast cells - deep purple Light Green - - light green - Gallocyanin dark blue - - - nucleic acids, Nissl granules - dark blue Romanowsky (e.g. Leishman's) blue pink acidophils - red, basophils - blue, azurophilic - purple Aldehyde Pararosanilin elastic fibres - purple
Haematoxylin and Eosin One of the most common staining techniques in pathology and histology. Acronym "H and E" stain. (H&E, HE). Haematoxylin Stains nuclei blue to dark-blue. Stains the matrix of hyaline cartilage, myxomatous, and mucoid material pale blue. Stains myelin weakly but is not noticeable if combined with eosin stain. combined with Orange G (H & Or. G.) instead of eosin, specifically stains the granules of acidophilic cells of the adenohypophysis (anterior pituitary). Eosin Stains cytoplasm pink to red; red blood cells are also bright red. Common counterstain to hematoxylin. Stain intensity varies with the formula as well as the fixative.

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Moodle - ANAT2241 - 2019

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ANAT2241 Support Links: The Virtual Microscope | Covering and Lining Epithelia | Glandular Epithelia | CT Components | CT Types | Bone, Bone Formation and Joints | Blood | Muscle | Nervous | Cardiovascular | Respiratory | Integumentary | GIT Organs | GIT 1 | GIT 2 | Lymphatic and Immune | Endocrine | Urinary | Female Reproductive | Male Reproductive | Eye | Histology Stains | Histology Drawings | Practicals Health and Safety 2013 | Moodle - 2018

ANAT2241 This practical support page content is not part of the science practical class and provides only background information for student self-directed learning purposes.

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Cite this page: Hill, M.A. (2024, May 5) Embryology ANAT2241 footer. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Template:ANAT2241_footer

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