Difference between revisions of "ANAT2341 Lab 2"

From Embryology
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{{Header}}
 
== 1. QUIZ ==
 
== 1. QUIZ ==
  
  
== 2. Guest Lecturer - Dr Fabien Delerue ==
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==2. Guest Lecturer - A/Prof Robert Gilchrist ==
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[[File:Oocyte BMP15 and GDF9 effects.jpg|thumb|Oocyte BMP15 and GDF9 effects PMID 25058588]]
 
{|
 
{|
| width=185px|[[File:Fabien deleRue profile photo.jpg|180px]]
 
  
Dr Fabien Delerue
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| [[File:Rob Gilchrist.jpg|left|150px|alt=Associate Professor Robert Gilchrist|link=https://research.unsw.edu.au/people/associate-professor-robert-bruce-gilchrist]]
| '''Manipulating the mouse embryo: from ES Cells to genome editing'''
 
<br>
 
Dr Delerue is currently in the Transgenic Animal Unit (TAU) his career spans over twenty years practice in animal handling, surgery, and generation of animal models of diseases. His research focuses on the production and characterization of new transgenic mouse models of human genetic disorders, using cutting-edge genome engineering techniques such as CRISPR/Cas9.
 
  
Links: [https://research.unsw.edu.au/people/dr-fabien-delerue UNSW Research Gateway]
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A/Prof Robert Gilchrist
|}
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| '''The Reproductive Technology Revolution'''
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<br><br>
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Dr Gilchristis head of the Oocyte Biology Research Unit (UNSW) his primary research interests are in the regulation of mammalian oocyte development and maturation, and the development of novel oocyte maturation techniques for infertility treatment.  
  
'''Lecture slides:''' [[File:Delerue.pdf]]
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[[Media:2017 Anatomy IVF and embryology lab.pdf|Lecture Slides]]
===References===
 
  
Delerue F & Ittner LM. Genome Editing in Mice Using CRISPR/Cas9: Achievements and Prospects
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<br><br>
Cloning and Transgenesis 2015, 4:135 doi:10.4172/2168-9849.1000135 [http://www.omicsgroup.org/journals/genome-editing-in-mice-using-crisprcas9-achievements-and-prospects-2168-9849-1000135.php?aid=50992]
 
  
:"Animal models are a powerful tool to understand the mechanisms underlying physiological and pathological processes in vivo. To date, mice remain the species most commonly used for genetic manipulation. The recent development of engineered endonucleases such as Zinc Finger Nucleases (ZFN), Transcription activator-like effector nucleases  (TALEN), and the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) offered easy, flexible, and fast alternative to ES-Cell based gene targeting. Thanks to multiple advantages, the CRISPR system superseded its predecessors and became a popular method for genome editing. Here, we review the latest techniques to apply CRISPR editing to the mouse genome, and emphasize on the current methods used in transgenic laboratories and subsequent achievements in mice."
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Links: [https://research.unsw.edu.au/people/associate-professor-robert-bruce-gilchrist UNSW Research Gateway] | [http://www.ncbi.nlm.nih.gov/pubmed/?term=Gilchrist+R%5BAuthor%5D PubMed]
 
 
Ke YD, van Hummel A, Stevens CH, Gladbach A, Ippati S, Bi M, Lee WS, Krüger S, van der Hoven J, Volkerling A, Bongers A, Halliday G, Haass NK, Kiernan M, Delerue F, Ittner LM.
Short-term suppression of A315T mutant human TDP-43 expression improves functional deficits in a novel inducible transgenic mouse model of FTLD-TDP and ALS. Acta Neuropathologica 2015 Nov;130(5):661-78 PubMed 26437864 [https://dx.doi.org/10.1007/s00401-015-1486-0]
 
 
 
Ittner A, Chua SW, Bertz J, Volkerling A, van der Hoven J, Gladbach A, Przybyla M, Bi M, van Hummel A, Stevens CH, Ippati S, Suh LS, Macmillan A, Sutherland G, Kril JJ, Silva APG, Mackay J, Poljak A, Delerue F, Ke YD, Ittner LM. Site-specific phosphorylation of tau inhibits amyloid-β toxicity in Alzheimer’s mice
 
Science 2016 Nov;354 (6314):904-908 doi: 10.1126/science.aah6205 [http://science.sciencemag.org/content/354/6314/904.full]
 
 
 
===Recommended Literature===
 
{|
 
| [[File:Manipulating_the_Mouse_Embryo.jpg|200px]]
 
| | [[File:Reproductive Engineering Techniques.jpg|200px]]
 
 
|-
 
|-
| [http://cshlpress.com/default.tpl?cart=147944968860031853&action=full&--eqskudatarq=982 Manipulating the Mouse Embryo: A Laboratory Manual (Fourth Edition)] By Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, & Andras Nagy.
 
| [http://card.medic.kumamoto-u.ac.jp/card/english/sigen/manual/onlinemanual.html Reproductive Engineering techniques in mice: Technical manual] Prof Naomi Nakagata, Center for Animal Resources and Development, Kumamoto University, Japan
 
 
|}
 
|}
  
===External Links===
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===Recent Articles===
{{External Links}}
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{{#pmid:27422885|PMID27422885}}
* NCBI Search - [http://www.ncbi.nlm.nih.gov/gquery/?term=CrispR NCBI databases - CRISPR] |  [http://www.ncbi.nlm.nih.gov/pubmed/?term=CrispR PubMed CRISPR] | [http://www.ncbi.nlm.nih.gov/pmc/?term=CrispR PubMed Centrap CRISPR]
 
* JoVE - [https://www.jove.com/video/55765/generation-genetically-modified-mice-through-microinjection]
 
  
<html5media height="460" width="640">https://www.youtube.com/watch?v=16g6fEup2pQ</html5media>
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:"The cyclic nucleotides, cAMP and cGMP, are the key molecules controlling mammalian oocyte meiosis. Their roles in oocyte biology have been at the forefront of oocyte research for decades and many of the long standing controversies in relation to the regulation of oocyte meiotic maturation are now resolved. It is now clear that the follicle prevents meiotic resumption through the actions of natriuretic peptides and cGMP inhibiting the hydrolysis of intra-oocyte cAMP and that the preovulatory gonadotrophin surge reverses these processes. The gonadotrophin surge also leads to a transient spike in cAMP in the somatic compartment of the follicle; research over the past 2 decades has conclusively demonstrated that this surge in cAMP is important for the subsequent developmental capacity of the oocyte. This is important, as oocyte in vitro maturation (IVM) systems practiced clinically do not recapitulate this cAMP surge in vitro, possibly accounting for the lower efficiency of IVM compared to clinical IVF. This review focuses in particular on this latter aspect - the role of cAMP/cGMP in the regulation of oocyte quality. We conclude that clinical practice of IVM should reflect this new understanding of the role of cyclic nucleotides, thereby creating a new generation of ART and fertility treatment options."
  
<br>
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{{#pmid:27248769|PMID27248769}}
==3. Student Group Projects==
 
I have now added (I hope) a discussion Forum for your group to Moodle available to only your group members.
 
  
<br>
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{{#pmid:27160446|PMID27160446}}
{{ANAT2341ProjectGroup2017table}}
 
  
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==3. Group Project==
  
<br>
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* Progress reports from groups.
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* [[Student_Page#Upload_Image_Tutorial|Image upload tutorial]]
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* Any issues?
  
{{Editing Links}}
 
 
<br>
 
<br>
  
 
{{2017ANAT2341 footer}}
 
{{2017ANAT2341 footer}}

Revision as of 14:17, 19 July 2018

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1. QUIZ

2. Guest Lecturer - A/Prof Robert Gilchrist

Oocyte BMP15 and GDF9 effects PMID 25058588
Associate Professor Robert Gilchrist

A/Prof Robert Gilchrist

The Reproductive Technology Revolution



Dr Gilchristis head of the Oocyte Biology Research Unit (UNSW) his primary research interests are in the regulation of mammalian oocyte development and maturation, and the development of novel oocyte maturation techniques for infertility treatment.

Lecture Slides



Links: UNSW Research Gateway | PubMed

Recent Articles

[1]

"The cyclic nucleotides, cAMP and cGMP, are the key molecules controlling mammalian oocyte meiosis. Their roles in oocyte biology have been at the forefront of oocyte research for decades and many of the long standing controversies in relation to the regulation of oocyte meiotic maturation are now resolved. It is now clear that the follicle prevents meiotic resumption through the actions of natriuretic peptides and cGMP inhibiting the hydrolysis of intra-oocyte cAMP and that the preovulatory gonadotrophin surge reverses these processes. The gonadotrophin surge also leads to a transient spike in cAMP in the somatic compartment of the follicle; research over the past 2 decades has conclusively demonstrated that this surge in cAMP is important for the subsequent developmental capacity of the oocyte. This is important, as oocyte in vitro maturation (IVM) systems practiced clinically do not recapitulate this cAMP surge in vitro, possibly accounting for the lower efficiency of IVM compared to clinical IVF. This review focuses in particular on this latter aspect - the role of cAMP/cGMP in the regulation of oocyte quality. We conclude that clinical practice of IVM should reflect this new understanding of the role of cyclic nucleotides, thereby creating a new generation of ART and fertility treatment options."

[2]

[3]

3. Group Project


 2017 ANAT2341 - Timetable | Course Outline | Group Projects | Moodle | Tutorial 1 | Tutorial 2 | Tutorial 3

Labs: 1 Fertility and IVF | 2 ES Cells to Genome Editing | 3 Preimplantation and Early Implantation | 4 Reproductive Technology Revolution | 5 Cardiac and Vascular Development | 6 CRISPR-Cas9 | 7 Somitogenesis and Vertebral Malformation | 8 Organogenesis | 9 Genetic Disorders | 10 Melanocytes | 11 Stem Cells | 12 Group

Lectures: 1 Introduction | 2 Fertilization | 3 Week 1/2 | 4 Week 3 | 5 Ectoderm | 6 Placenta | 7 Mesoderm | 8 Endoderm | 9 Research Technology | 10 Cardiovascular | 11 Respiratory | 12 Neural crest | 13 Head | 14 Musculoskeletal | 15 Limb | 16 Renal | 17 Genital | 18 Endocrine | 19 Sensory | 20 Fetal | 21 Integumentary | 22 Birth | 23 Stem cells | 24 Revision

 Student Projects: 1 Cortex | 2 Kidney | 3 Heart | 4 Eye | 5 Lung | 6 Cerebellum
  1. Gilchrist RB, Luciano AM, Richani D, Zeng HT, Wang X, Vos MD, Sugimura S, Smitz J, Richard FJ & Thompson JG. (2016). Oocyte maturation and quality: role of cyclic nucleotides. Reproduction , 152, R143-57. PMID: 27422885 DOI.
  2. Robertson DM, Gilchrist RB, Ledger WL & Baerwald A. (2016). Random start or emergency IVF/in vitro maturation: a new rapid approach to fertility preservation. Womens Health (Lond) , 12, 339-49. PMID: 27248769 DOI.
  3. Russell DL, Gilchrist RB, Brown HM & Thompson JG. (2016). Bidirectional communication between cumulus cells and the oocyte: Old hands and new players?. Theriogenology , 86, 62-8. PMID: 27160446 DOI.