Course Links
Moodle - ANAT2241 - 2019
- Histology Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | ANAT2241 Support | Histology | Histology Stains | Embryology Glossary
Common Histology Stains
|
Histology Stains - Common Stains and Their Reactions
|
Stain
|
Nucleus
|
Cytoplasm
|
Collagen
|
RBCs
|
Other
|
Haematoxylin
|
blue
|
-
|
-
|
-
|
mucins - light blue
|
Eosin
|
-
|
pink
|
pale pink
|
bright red
|
colloid - pinkmuscle - red
|
Iron Haematoxylin
|
blue/black
|
-
|
-
|
-
|
|
Van Gieson
|
-
|
brown/yellow
|
red
|
yellow
|
muscle: yellow/browncartilage - pink
|
Verhoeff's Elastin
|
black
|
-
|
-
|
-
|
elastic fibres - black
|
Tartrazine
|
-
|
yellow
|
yellow
|
yellow
|
|
Silver Impregnation
|
-
|
-
|
grey/brown
|
-
|
reticular fibres - black
|
Methyl Green
|
dark green
|
light green
|
light green
|
green
|
|
Nuclear Fast Red
|
red
|
pink
|
pink
|
pink
|
|
Gomori's Trichrome
|
purple/red
|
purple
|
green
|
red
|
keratin - redmuscle - purple/red
|
Heidenhain's Azan
|
red
|
purple/red
|
deep blue
|
red
|
muscle - red
|
Osmium Tetroxide
|
-
|
-
|
brown
|
brown
|
myelin, lipids - black
|
Alcian Blue
|
-
|
-
|
-
|
-
|
mucins, - blue
|
Periodic acid-Schiff (PAS)
|
-
|
-
|
pink
|
-
|
mucins, glycogen, glycocalyx - magenta
|
Phosphotungstic Acid-Hematoxylin (PTAH)
|
blue
|
-
|
red
|
blue
|
muscle bands - blue
|
Masson's Trichrome
|
blue/black
|
red
|
green/blue
|
red
|
cartilage, mucins - blue or green; muscle - red
|
Luxol Fast Blue
|
-
|
-
|
-
|
variable
|
myelin - blue
|
Aldehyde Fuchsin
|
-
|
-
|
-
|
-
|
elastic fibres, mast cells - deep purple
|
Light Green
|
-
|
-
|
light green
|
-
|
|
Gallocyanin
|
dark blue
|
-
|
-
|
-
|
nucleic acids, Nissl granules - dark blue
|
Romanowsky (e.g. Leishman's)
|
blue
|
pink
|
|
|
acidophils - red, basophils - blue, azurophilic - purple
|
Aldehyde Pararosanilin
|
|
|
|
|
elastic fibres - purple
|
|
Haematoxylin and Eosin
|
One of the most common staining techniques in pathology and histology. Acronym "H and E" stain. (H&E, HE).
|
Haematoxylin
- Stains nuclei blue to dark-blue.
- Stains the matrix of hyaline cartilage, myxomatous, and mucoid material pale blue.
- Stains myelin weakly but is not noticeable if combined with eosin stain.
- combined with Orange G (H & Or. G.) instead of eosin, specifically stains the granules of acidophilic cells of the adenohypophysis (anterior pituitary).
|
Eosin
- Stains cytoplasm pink to red; red blood cells are also bright red.
- Common counterstain to hematoxylin.
- Stain intensity varies with the formula as well as the fixative.
|
|
Practical Support
- Pages can be accessed from any internet connected computer.
ANAT2241 Support Links: The Virtual Microscope | Covering and Lining Epithelia | Glandular Epithelia | CT Components | CT Types | Bone, Bone Formation and Joints | Muscle | Nervous | Blood | Eye | Cardiovascular | Respiratory | Integumentary | GIT Organs | GIT 1 | GIT 2 | Lymphatic and Immune | Endocrine | Urinary | Female Reproductive | Male Reproductive | Histology Stains | Histology Drawings | Practicals Health and Safety 2013 | Moodle - 2019
ANAT2241 This practical support page content is not part of the science practical class and provides only background information for student self-directed learning purposes.
Cite this page: Hill, M.A. (2024, June 16) Embryology ANAT2241 footer. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Template:ANAT2241_footer
- What Links Here?
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G