User:Z5020317
--Mark Hill (talk) 10:47, 6 August 2015 (AEST) Thanks for setting up your page. We will be talking more about this in the Practical on Friday.
Lab 1 Assessment
Your Lab assessment now requires you to find a 2 recent research references on fertilisation or in vitro fertilisation.
Paste each reference on your page, as shown in the class.
Write below each reference a brief summary of the research article methods and findings.
The summary for each need not be more than 3-4 paragraphs in length.
This will need to be completed before next weeks laboratory.
The use of r-hFSH in treatment of idiopathic male factor infertility before ICSI.
look at this [1]
PMID 26166637
The objective of this investigations was to evaluate whether or not the pre-treatment use of Recombinant Human Follicle Stimulating Hormone (r-hFSH), could be used to treat common male infertility issues as Idiopathic Oligozoospermia, to improve their clinical results of Intracytoplasmic Sperm Injection (ICSI).
They selected a sample of 82 couples that were infertile due to the Idiopathic male factor, from the Biofertility Center in Rome, Italy between May 2013- April 2014 and randomly selected a treatment group of 36 males, Group A, and control group of 46 men, Group. The men in treatment Group A were subcutaneously injected with 150 IU of R-hFSH; Gonad F© (recombinant Human FSH) 3 times per week, for a 3 month period. The subjects in the control Group B were injected with a placebo and therefore received no treatment. After the three month period all 82 couples underwent a cycle of ICSI. In each group fertilisation, rate of implantation, rate of clinical pregnancy and the rate of miscarriage were assessed.
They found that the rate fertilisation was comparable in both treatment groups, although treatment group A had a significantly higher implantation rate and clinical pregnancy rate as well as a statistically significant lower rate of miscarriage (P<0.05). The conclusion that they drew was that the treatment use of r-hFSH to treat Idiopathic males infertility prior to ICSI can improve the clinical rate of pregnancy, and increase the rate of implantation as well as decrease the chances of early pregnancy loss.
Effect of hepatitis C virus infection on the outcomes of in vitro fertilization.
look at this [2]
PMID 26131230
The objective of this experiment was to investigate the effects that the Hepatitis C Virus (HCV) infection has upon the outcome In Vitro Fertilisation (IVF). From January 2008 - December 2013 a large sample of data was collected from couples opting for IVF at the Centre for Reproductive Medicine, General Hospital of Tianjin Medical University. These 1424 couples were tested for the HCV antibody (HCV-Ab) and were then separated into 3 groups. Group A contained 90 couples where the female tested HCV positive, Group B had 78 couples in which the male partner tested HCV positive and Group C - was the control group, of 1256 couples that both tested negative for HCV. The control group was also matched in age and in accordance with the IVF ovarian hyper stimulation protocol. These couples then underwent IVF and the results were closely monitored.
Initially the sperm was collected and its concentration, volume, motility, normal sperm morphology percentage (NMP) and Teratozoospermia index (TZI) were assessed, and again tested for the presence of HCV-Ab. The women were tested with Follicle Stimulating Hormone (FSH) on days 2-4 of their Menstrual cycle, within the 6 months of IVF treatment. They underwent Ovarian Hyperstilumation, and intramuscular injections of chronic Gonadotropin to mature the Oocytes. Transvaginal collection of mature Oocytes occurred roughly 36 hours later. 3 days later 2 high quality embryos were then transferred back into the uterus and oral progesterone was administered to support the Luteal phase of menstruation cycle. 14 days after the embryo transfer, pregnancy can be detected by testing for increasing levels of hCG in the blood, and can later, be clinically diagnosed through the presence of gastrointestinal sac during an abdominal ultrasound.
They found that all the groups (HVC positive male, HCV positive female , HCV negative couples) had similar results in terms of age, ovarian response, endometrial thickness, duration of Gonadotropin administration, embryo transfer and day of hCG administration, and no obvious differences within the sperm parameters; motility, concentration , PR differences, volume and NMP, TZI. They proceeded to investigate the effects of HCV on pregnancy and found no notable differences on pregnancy rates per cycle. They came to the conclusion that HVC had no effect upon IVF treatment or pregnancy.
Lab 2 Assessment
Upload a research image using the guide information below. The image uploaded for your individual assessment can relate to your project or from fertilisation to week 3 of development (upload only a single image).
Add that image to your own individual page (see Images) including an image title and its reference link.
Uploading Images in 5 Easy Steps | ||
---|---|---|
First Read the help page Images and Copyright Tutorial.
Students cannot delete images once uploaded. You will need to email me with the full image name and request deletion, that I am happy to do with no penalty if done before I assess. Non-Table version of this page
|
look at this [3]
PMID 26250560
Ovarian stem Cell Schematic [4]
PMID 26250560
Lab 3 Assessment
In Lab 3 you were asked to divide the group project among your members and to research your allocated project component.
You will need to demonstrate that you have actively researched your project topic over the last week by having your section heading appear on the project page and have at least 3 research/review articles listed under that sub-heading.
The same content should be passed on your own individual assessment page.
Prenatal Diagnosis
Prenatal diagnosis is the screening process that tests an early fetus for overall growth, complications of pregnancy, birth defects and chromosomal or genetic abnormalities within the first 2 trimesters. It aims to provide the parents with as information as possible to help them make an informed decision about the infants quality of life. In 90-95% of the cases negative outcomes occur; confirming the healthy state of the fetus, should a genetic abnormality be present, it provides the parents with the opportunity to investigate further with other tests (PGD), and possible fetal therapeutic treatments available as well plan and prepare for the disabled infant or to terminate the pregnancy.
Indicators for prenatal screening include: maternal ages > 35 years paternal ages > 50-55 history of 2+ miscarriages previous pregnancy or family history of a preexisting genetic or chromosomal disorder suspected carriers of genetic disorders positive test results within the first 2 trimesters family history of neural tube or other birth defects
look at this [5]
Lab Attendance
--Z5020317 (talk) 13:46, 7 August 2015 (AEST)
--Z5020317 (talk) 13:15, 14 August 2015 (AEST)
References
--Mark Hill (talk) 10:21, 13 August 2015 (AEST) I have put the correct code for the reference list below.
- ↑ <pubmed>26166637</pubmed>
- ↑ <pubmed>26131230</pubmed>
- ↑ <pubmed>26250560</pubmed>
- ↑ <pubmed>26250560</pubmed>| [1]
- ↑ <Carbonell, R. Shinners, A. Amor, D. Mark, D. (2015) Report on Cutting edge prenatal screening technology to become available in Australia: Prepared for ABC news PM with Mark Colvin. Retrieved from [[2]]>
Please do not use your real name on this website, use only your student number.
- 2015 Course: Week 2 Lecture 1 Lecture 2 Lab 1 | Week 3 Lecture 3 Lecture 4 Lab 2 | Week 4 Lecture 5 Lecture 6 Lab 3 | Week 5 Lecture 7 Lecture 8 Lab 4 | Week 6 Lecture 9 Lecture 10 Lab 5 | Week 7 Lecture 11 Lecture 12 Lab 6 | Week 8 Lecture 13 Lecture 14 Lab 7 | Week 9 Lecture 15 Lecture 16 Lab 8 | Week 10 Lecture 17 Lecture 18 Lab 9 | Week 11 Lecture 19 Lecture 20 Lab 10 | Week 12 Lecture 21 Lecture 22 Lab 11 | Week 13 Lecture 23 Lecture 24 Lab 12 | 2015 Projects: Three Person Embryos | Ovarian Hyper-stimulation Syndrome | Polycystic Ovarian Syndrome | Male Infertility | Oncofertility | Preimplantation Genetic Diagnosis | Students | Student Designed Quiz Questions | Moodle page
Glossary Links
- Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link
Here is the List