User:Z3463514
Lab Attendance
Lab 1 --Z3463514 (talk) 13:45, 7 August 2015 (AEST)
Lab 2 --Z3463514 (talk) 13:21, 14 August 2015 (AEST)
Lab 3 --Z3463514 (talk) 12:28, 21 August 2015 (AEST)
Lab 4 --Z3463514 (talk) 12:12, 28 August 2015 (AEST)
Lab 5 --Z3463514 (talk) 12:13, 4 September 2015 (AEST)
Lab 6 --Z3463514 (talk) 12:21, 11 September 2015 (AEST)
Lab 7 --Z3463514 (talk) 12:08, 18 September 2015 (AEST)
Lab 8 --Z3463514 (talk) 12:04, 25 September 2015 (AEST)
Lab 9 --Z3463514 (talk) 12:07, 9 October 2015 (AEDT)
Lab 1 Assessment
Article 1 PMID 26131222
The aim of this article was to determine the impact of oxygen concentration during in vitro culture of human oocytes and embryo on fertilisation, implantation, pregnancy, gestation and abortion rates. Women between 20-48 years old who are seeking for infertility treatments and intracytoplasmic sperm injection participated in this experiment. Embryos were randomly allocated for incubation under three different oxygen conditions, the first group was subjected to 20% Oxygen in air. The second group was initially subjected to 20% Oxygen in air, then on the following day (day 2) 5% Carbon Dioxide and 90% Nitrogen gas was introduced into the system while Oxygen was decreased to 5%. Finally, the third group was subjected to 5% Carbon Dioxide gas and 90% Nitrogen gas throughout the experiment.
To determine its ability to yield a successful pregnancy, the embryo must be cultured and incubated for 2-6 days in a defined medium.
Embryos cultured in 5% Oxygen in air presented highest rates of fertilization and implantation compared to those incubated in 20% Oxygen in air. Meanwhile, embryos cultured in 20% Oxygen in air also presented high rates of fertilisation, high quality embryo and implantation. Intracytoplasmic sperm injection derived embryos cultured in 20% Oxygen in air resulted in lower rates of cleavage compared to those of from the second group, from 20% - 5% Oxygen in air. These results were consistent with the data previously published.[1]
Article 2 PMID 26238449
The objective of this article was to evaluate in vitro maturation, IVM in sub-fertile women with polycystic ovarian syndrome undergoing IVF, by comparing outcomes with a control group of non-polycystic ovarian syndrome women. IVM involves the in vitro culture of immature oocytes from metaphase II, when the oocyte is considered to be fully mature to undergo fertilisation. This could be a groundbreaking procedure for women suffering polycystic ovarian syndrome as these gametes has expressed their maturational and developmental competence after their retrieval from the ovaries.
A total of 268 patients suffering with polycystic ovarian syndrome, 100 PCO patients and 400 controls were included in the investigation. The analysis provided information suggesting that IVM is a preferable approach in treating women with PCOS during an IVF cycle compared to those without the syndrome. Thus is was concluded that IVM was more efficient as a treatment option in terms of clinical pregnancy, implantation and cycle cancellation rate in women suffering PCOS. [2]
--Mark Hill (talk) 10:46, 16 September 2015 (AEST) Good summaries of these 2 articles. The second article is particularly interesting. (5/5)
Lab 2 Assessment - Images
Uploading Images in 5 Easy Steps | ||
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First Read the help page Images and Copyright Tutorial.
Students cannot delete images once uploaded. You will need to email me with the full image name and request deletion, that I am happy to do with no penalty if done before I assess. Non-Table version of this page
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Deer mice oocytes at various stages of development in vitro [3]
PMID 23457518
Copyright: © 2013 Choi, He. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
--Mark Hill (talk) 10:46, 16 September 2015 (AEST) You have uploaded the image OK and given it a suitable name. You have not included the reference, copyright and student template in the image summery box (copyright not required on your page here). I have updated your image summary box for you to demonstrate how this information should appear with uploaded images. (3/5)
Lab 3 Assessment
1. Shiraishi, K., Ohmi, C., Shimabukuro, T., & Matsuyama, H. (2012). Human chorionic gonadotrophin treatment prior to microdissection testicular sperm extraction in non-obstructive azoospermia. Human reproduction, 27(2), 331-339. PMID 22128297
2. Irvine, D. S. (1998). Epidemiology and aetiology of male infertility. Human Reproduction, 13(suppl 1), 33-44. PMID 9663768
3. Lotti, F., & Maggi, M. (2015). Ultrasound of the male genital tract in relation to male reproductive health. Human reproduction update, 21(1), 56-83. PMID 25038770
--Mark Hill (talk) 10:50, 16 September 2015 (AEST) These references relate to your group project work. You could have used the reference template here and perhaps given a sentence explaining the relevance to the topic. (5/5)
Lab 4 Assessment - Quiz Questions
Early Vascular Development
--Mark Hill (talk) 10:50, 16 September 2015 (AEST) Q1 does not really test the students knowledge on the topic and your explanation in the answer also does not help those who may have gotten the question incorrect. You need to have gone through each option with an explanation here as well as perhaps links to useful resources. Q2 "NOT correct" type of questions are always difficult to design correctly without just "tricking" the student. In particular your third option basically sets up option 4 as the only correct answer. Once again more work on your revealed answer required. Q3 is really a very silly question and does not test an embryology student. (7/10)
Lab 5 Assessment
What is the difference between gastroschisis and omphalocele?
Gastroschisis and omphalocele are congenital abnormalities of the abdominal wall and are the most common anterior abdominal wall abnormality in infants. Gastrochisis is a congenital malformation which occurs in the abdominal wall due to incomplete closure of the lateral folds during the initial gestation. Unlike omphalocele, the exposed viscera lateral, usually to the right, to the closed umbilical ring does not have a covering sac or remnant membrane.[4] Through thorough research, it appeared that the gastroschisis could be the result of amniotic damage, poor prenatal care, maternal infections and younger maternal age. [5]
Omphalocele is a congenital defect which is thought to occur during the process of body folding. It is where the umbilical cord and intestinal tract are extruded or herniated through the umbilical ring with a covering sac or sometimes with its remnants of peritoneum and amnion. Research has observed that the formation of an omphalocele is highly associated with chromosome abnormalities such as trisomies 18 and 13. The clinical discrepancy between the two malformations were based upon its location, size and whether the covering sac or remnant is present or absent. [6] [7] It was observed that infants with omphalocele were more susceptible to have other anomalies, and were diagnosed with pulmonary hypertension. [8]
During the first trimester, vaginal ultrasound may be able to diagnose whether a foetus of as early as 12 weeks suffers from gastrochisis and omphalocele. The ultrasound may assist paediatric surgeons to identify the contents of the herniation and malformation.
--Mark Hill (talk) 11:00, 16 September 2015 (AEST) Correct. (5/5)
Lab 8 Assessment - Peer Assessment
Group Project 1
It’s great to begin with a introductory video which defines your topic. I do suggest finding a reference for the first paragraph for the introduction. Besides that, references have been cited correctly and shows that you have conducted extensive research, but remember to reference as you add information ( [##] ). I think you guys did a great job with the heading and subheadings; it shows us that you have done extensive literature research, and have came to a conclusion as to what information was relevant. Just a grammatical error made in “Timeline of Mitocondrial Donation” which is missing a H in mitochonidral. I suggest proof reading all the text before uploading! This will make it easier for the audience to understand and also for yourself! As to the “Benefit” heading, I think it will be a good idea to add information and case studies on disadvantages towards three person embryos.
Nice to see that you guys have included a timeline, this shows the progress made throughout the years. But I think there is still information that can be added into this area; for example: different possible approaches or more controversial issues that has emerged. “Technical Progression” is an impressive choice of heading; I found it very interesting to read. The cytoplasmic transfer images used were great! They were very easy to understand. The “Timeline” under “Cytoplasmic transfer” could be merged with the history timeline heading above.
Overall, I think more information and content needs to be added under all the headings and subheadings. To make it easier for the audience to read, I suggest adding detailed images, tables and flowcharts. It will be more eye-catching for readers and will keep them interested. I see that you guys have a table under “Prohibited Section” however that just leads to another link, rather than having the link there; I think it would be a great improvement if there is a short summary of all the sources found.
I think your page is organised and formatted very well! With more information/content, detailed diagrams and tables; it will further improve your Wiki Page! Good Luck.
Group Project 2
Group Project 3
Group Project 5
Group Project 6
References
- ↑ <pubmed>25131222</pubmed>
- ↑ <pubmed>26238449</pubmed>
- ↑ <pubmed>23457518</pubmed>| [1]
- ↑ Júnior, E. A., Rolo, L. C., Tonni, G., Haeri, S., & Ruano, R. (2015). Assessment of fetal malformations in the first trimester of pregnancy by three-dimensional ultrasonography in the rendering mode. Pictorial essay. Medical ultrasonography, 17(1), 109. PMID 25745664
- ↑ Bargy, F., & Beaudoin, S. (2014). Comprehensive Developmental Mechanisms in Gastroschisis. Fetal diagnosis and therapy, 36(3), 142-149. PMID25171094
- ↑ Calzolari, E., Bianchi, F., Dolk, H., & Milan, M. (1995). Omphalocele and gastroschisis in Europe: a survey of 3 million births 1980–1990. American journal of medical genetics, 58(2), 187-194.
- ↑ Júnior, E. A., Rolo, L. C., Tonni, G., Haeri, S., & Ruano, R. (2015). Assessment of fetal malformations in the first trimester of pregnancy by three-dimensional ultrasonography in the rendering mode. Pictorial essay. Medical ultrasonography, 17(1), 109. PMID 25745664
- ↑ Corey, K. M., Hornik, C. P., Laughon, M. M., McHutchison, K., Clark, R. H., & Smith, P. B. (2014). Frequency of anomalies and hospital outcomes in infants with gastroschisis and omphalocele. Early human development, 90(8), 421-424. PMID 24951080
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