|Embryology - 17 Sep 2019 Expand to Translate|
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Organoids stem cell derived self-organized three-dimensional in vitro tissue cultures. These cultures have been be manipulated to replicate some of the complexity of an in vivo organ, or to produce only certain types of organ related cells. This stem cell research technique has many potential applications in understanding tissue and organ development, clinical therapeutics and cancer medicine.
|Stem Cell Links: Introduction | Timeline | Placental Cord Blood | Adult | Induced | Yamanaka Factors | Somatic Cell Nuclear Transfer | Ethics | Organoids | Category:Stem Cell|
Some Recent Findings
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Inner Ear Organoids
Modulation of Wnt Signaling Enhances Inner Ear Organoid Development in 3D Culture
- "Stem cell-derived inner ear sensory epithelia are a promising source of tissues for treating patients with hearing loss and dizziness. We recently demonstrated how to generate inner ear sensory epithelia, designated as inner ear organoids, from mouse embryonic stem cells (ESCs) in a self-organizing 3D culture. Here we improve the efficiency of this culture system by elucidating how Wnt signaling activity can drive the induction of otic tissue. We found that a carefully timed treatment with the potent Wnt agonist CHIR99021 promotes induction of otic vesicles-a process that was previously self-organized by unknown mechanisms. The resulting otic-like vesicles have a larger lumen size and contain a greater number of Pax8/Pax2-positive otic progenitor cells than organoids derived without the Wnt agonist. Additionally, these otic-like vesicles give rise to large inner ear organoids with hair cells whose morphological, biochemical and functional properties are indistinguishable from those of vestibular hair cells in the postnatal mouse inner ear. We conclude that Wnt signaling plays a similar role during inner ear organoid formation as it does during inner ear development in the embryo."
Kidney Organoids: A Translational Journey
- "Human pluripotent stem cells (hPSCs) are attractive sources for regenerative medicine and disease modeling in vitro. Directed hPSC differentiation approaches have derived from knowledge of cell development in vivo rather than from stochastic cell differentiation. Moreover, there has been great success in the generation of 3D organ-buds termed 'organoids' from hPSCs; these consist of a variety of cell types in vitro that mimic organs in vivo. The organoid bears great potential in the study of human diseases in vitro, especially when combined with CRISPR/Cas9-based genome-editing. We summarize the current literature describing organoid studies with a special focus on kidney organoids, and discuss goals and future opportunities for organoid-based studies."
Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation.
- "Adult somatic tissues have proven difficult to expand in vitro, largely because of the complexity of recreating appropriate environmental signals in culture. We have overcome this problem recently and developed culture conditions for adult stem cells that allow the long-term expansion of adult primary tissues from small intestine, stomach, liver and pancreas into self-assembling 3D structures that we have termed 'organoids'. We provide a detailed protocol that describes how to grow adult mouse and human liver and pancreas organoids, from cell isolation and long-term expansion to genetic manipulation in vitro. Liver and pancreas cells grow in a gel-based extracellular matrix (ECM) and a defined medium. The cells can self-organize into organoids that self-renew in vitro while retaining their tissue-of-origin commitment, genetic stability and potential to differentiate into functional cells in vitro (hepatocytes) and in vivo (hepatocytes and endocrine cells). Genetic modification of these organoids opens up avenues for the manipulation of adult stem cells in vitro, which could facilitate the study of human biology and allow gene correction for regenerative medicine purposes. The complete protocol takes 1-4 weeks to generate self-renewing 3D organoids and to perform genetic manipulation experiments."
The use of brain organoids to investigate neural development and disease
- "Understanding the development and dysfunction of the human brain is a major goal of neurobiology. Much of our current understanding of human brain development has been derived from the examination of post-mortem and pathological specimens, bolstered by observations of developing non-human primates and experimental studies focused largely on mouse models. However, these tissue specimens and model systems cannot fully capture the unique and dynamic features of human brain development. Recent advances in stem cell technologies that enable the generation of human brain organoids from pluripotent stem cells (PSCs) promise to profoundly change our understanding of the development of the human brain and enable a detailed study of the pathogenesis of inherited and acquired brain diseases."
- Xu H, Lyu X, Yi M, Zhao W, Song Y & Wu K. (2018). Organoid technology and applications in cancer research. J Hematol Oncol , 11, 116. PMID: 30219074 DOI.
- Park SE, Georgescu A & Huh D. (2019). Organoids-on-a-chip. Science , 364, 960-965. PMID: 31171693 DOI.
- Takebe T & Wells JM. (2019). Organoids by design. Science , 364, 956-959. PMID: 31171692 DOI.
- Di Lullo E & Kriegstein AR. (2017). The use of brain organoids to investigate neural development and disease. Nat. Rev. Neurosci. , 18, 573-584. PMID: 28878372 DOI.
- Morizane R & Bonventre JV. (2017). Kidney Organoids: A Translational Journey. Trends Mol Med , 23, 246-263. PMID: 28188103 DOI.
- Clevers H. (2016). Modeling Development and Disease with Organoids. Cell , 165, 1586-1597. PMID: 27315476 DOI.
- DeJonge RE, Liu XP, Deig CR, Heller S, Koehler KR & Hashino E. (2016). Modulation of Wnt Signaling Enhances Inner Ear Organoid Development in 3D Culture. PLoS ONE , 11, e0162508. PMID: 27607106 DOI.
- Held M, Santeramo I, Wilm B, Murray P & Lévy R. (2018). Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy. PLoS ONE , 13, e0199918. PMID: 30048451 DOI.
- Broutier L, Andersson-Rolf A, Hindley CJ, Boj SF, Clevers H, Koo BK & Huch M. (2016). Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation. Nat Protoc , 11, 1724-43. PMID: 27560176 DOI.
van den Hurk M & Bardy C. (2019). Single-cell multimodal transcriptomics to study neuronal diversity in human stem cell-derived brain tissue and organoid models. J. Neurosci. Methods , 325, 108350. PMID: 31310823 DOI.
Pekkanen-Mattila M, Pelto-Huikko M, Kujala V, Suuronen R, Skottman H, Aalto-Setälä K & Kerkelä E. (2010). Spatial and temporal expression pattern of germ layer markers during human embryonic stem cell differentiation in embryoid bodies. Histochem. Cell Biol. , 133, 595-606. PMID: 20369364 DOI.
May 2006 "stem cell" 154,176 reference articles of which 16,449 were reviews.
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- StemBook - Table of Contents
- International Society for Stem Cell Research (ISSCR) is an independent, nonprofit organization formed in 2002 to foster the exchange of information on stem cell research.
- University of Michigan Stem Cells Explained
- Transcript of discussion on ABC Radio (Dr. J Kahn , Dr. JWagner) on Genetic Technology And Ethics
- A brief article on Cord Blood stem cells and their therapeutic potential from the BBC.
- Monash University (Australia) Monash Immunology and Stem Cell Laboratories (MISCL)
- Europe - ESTOOLS DATA@HAND "resource contains human gene expression array data from 97 GEO and ArrayExpress sample sets, which involve altogether 1674 Affymetrix, Illumina and Agilent arrays. The source of the biological samples is mainly pluripotent stem cells, their differentiated progeny, and their parent cells. All data has been preprocessed so as to enable computational analysis with analysis workflows and tools provided."
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Cite this page: Hill, M.A. (2019, September 17) Embryology Organoids. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Organoids
- © Dr Mark Hill 2019, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G