ANAT2241 Connective Tissue Components

From Embryology
ANAT2241 This practical support page content is not part of the virtual science practical class and provides additional information for student self-directed learning purposes. 2018 - Moodle ANAT2241 | Virtual slides

General Objective

To recognise various components of connective tissue and understand their functions.

Specific Objectives

  1. To understand the structure and functions of the cell types found in connective tissues: fibroblasts and fibrocytes, macrophages, plasma cells, mast cells, lymphocytes.
  2. To understand the connective tissue fiber constitution and function of reticular, elastic, areolar or loose fibrous tissue and dense fibrous tissue.
  3. To recognise the different types of general connective tissue, loose, dense irregular, dense regular.
  4. To understand the origin of tissue fluid and intercellular ground substance.

Learning Activities

Examine the following virtual slides and identify, draw & label features and note their function.

Connective Tissue Components | Histology Drawings

Mesentery

Loose irregular connective tissue

Low Power view High Power view
Mesentery histology 02.jpg Mesentery histology 01.jpg

Extracellular Matrix - collagen fibres, elastic fibres

Cells - fibroblasts, mast cells

Reticular Fibres

Silver stain for reticular fibres.

Liver-reticular fibre.jpg


Upper image shows the liver macrophages (Kupffer cells)

Liver- Kupffer cell and reticular fibre.jpg

Lower image shows the distribution of reticular fibres within the liver.


Elastic Fibres

Skin

Skin elastic fibres and collagen bundles.

Integumentary histology 10.jpgAdult skin histology 04.jpg

Artery

Artery histology 04.jpg

Adipose Tissue

Adult skin histology 02.jpg Pacinian corpuscle histology 01.jpg

White Adipose

White adipose 01.jpg White adipose 02.jpg


Brown Adipose

Brown adipose histology.jpg

Cartilage

Fetal cartilage 01.jpg Endochondral ossification.jpg

Developing cartilage

Hyaline

Elastic

Epiglottis cartilage 01.jpg Epiglottis cartilage 02.jpg

Elastic cartilage (elastin stain)

Fibrocartilage

Fibrous cartilage 01.jpg Fibrous cartilage 02.jpg


Tendon

Skeletal muscle histology 044.jpg



Course Links

Histology Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | ANAT2241 Support | Histology | Histology Stains | Embryology Glossary
Common Histology Stains  
Histology Stains - Common Stains and Their Reactions
Stain
Nucleus
Cytoplasm
Collagen
RBCs
Other
Haematoxylin
blue
-
-
-
mucins - light blue
Eosin
-
pink
pale pink
bright red
colloid - pinkmuscle - red
Iron Haematoxylin
blue/black
-
-
-
Van Gieson
-
brown/yellow
red
yellow
muscle: yellow/browncartilage - pink
Verhoeff's Elastin
black
-
-
-
elastic fibres - black
Tartrazine
-
yellow
yellow
yellow
Silver Impregnation
-
-
grey/brown
-
reticular fibres - black
Methyl Green
dark green
light green
light green
green
Nuclear Fast Red
red
pink
pink
pink
Gomori's Trichrome
purple/red
purple
green
red
keratin - redmuscle - purple/red
Heidenhain's Azan
red
purple/red
deep blue
red
muscle - red
Osmium Tetroxide
-
-
brown
brown
myelin, lipids - black
Alcian Blue
-
-
-
-
mucins, - blue
Periodic acid-Schiff (PAS)
-
-
pink
-
mucins, glycogen, glycocalyx - magenta
Phosphotungstic Acid-Hematoxylin (PTAH)
blue
-
red
blue
muscle bands - blue
Masson's Trichrome
blue/black
red
green/blue
red
cartilage, mucins - blue or green; muscle - red
Luxol Fast Blue
-
-
-
variable
myelin - blue
Aldehyde Fuchsin
-
-
-
-
elastic fibres, mast cells - deep purple
Light Green
-
-
light green
-
Gallocyanin
dark blue
-
-
-
nucleic acids, Nissl granules - dark blue
Romanowsky (e.g. Leishman's)
blue
pink
acidophils - red, basophils - blue, azurophilic - purple
Aldehyde Pararosanilin elastic fibres - purple
Haematoxylin and Eosin
One of the most common staining techniques in pathology and histology. Acronym "H and E" stain. (H&E, HE).


Haematoxylin
  • Stains nuclei blue to dark-blue.
  • Stains the matrix of hyaline cartilage, myxomatous, and mucoid material pale blue.
  • Stains myelin weakly but is not noticeable if combined with eosin stain.
  • combined with Orange G (H & Or. G.) instead of eosin, specifically stains the granules of acidophilic cells of the adenohypophysis (anterior pituitary).
Eosin
  • Stains cytoplasm pink to red; red blood cells are also bright red.
  • Common counterstain to hematoxylin.
  • Stain intensity varies with the formula as well as the fixative.

Virtual slides

Moodle - ANAT2241 - 2018

Pages require student zpass to access.

The Virtual Microscope | Covering and Lining Epithelia | Glandular Epithelia | Connective Tissue Components | Connective Tissue Types | Bone, Bone Formation and Joints | Blood | Muscle Tissue | Nervous Tissue | Cardiovascular System | Respiratory System | Integumentary System (skin) | Liver, Gallbladder, and Pancreas | Gastro-Intestinal System I | Gastro-Intestinal System II | Lymphatic Tissue and Immune System | Endocrine System | Urinary System | Female Reproductive System | Male Reproductive System | Special Sense Organ: The Eye


Practical support

Pages can be accessed from any internet connected computer.

ANAT2241 Support Links: The Virtual Microscope | Covering and Lining Epithelia | Glandular Epithelia | CT Components | CT Types | Bone, Bone Formation and Joints | Blood | Muscle | Nervous | Cardiovascular | Respiratory | Integumentary | GIT Organs | GIT 1 | GIT 2 | Lymphatic and Immune | Endocrine | Urinary | Female Reproductive | Male Reproductive | Eye | Histology Stains | Histology Drawings | Practicals Health and Safety 2013 | Moodle - 2018


ANAT2241 This practical support page content is not part of the science practical class and provides only background information for student self-directed learning purposes.


Cite this page: Hill, M.A. (2018, November 14) Embryology ANAT2241 Connective Tissue Components. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/ANAT2241_Connective_Tissue_Components

What Links Here?
© Dr Mark Hill 2018, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G