|ANAT2241 This practical support page content is not part of the science practical class and provides only background information for student self-directed learning purposes. 2018 Coordinator notice - Virtual slide links shown on these support pages are now transferred to the new Moodle links.|
- 1 General Objective
- 2 Learning Activities
- 3 Histology Stains
- 4 Blood Histology
- 5 Blood Cell Numbers
- 6 Terms
- 7 Course Links
|Blood Smear Slide (unstained)||Blood Smear Slide (stained)|
To know the formed elements of human blood and to appreciate that blood could be regarded as a “tissue” in which plasma constitutes the intercelluar substance.
- To differentiate blood cells on the basis of morphology and staining properties.
- To understand the principles of blood formation and the tissues and cells involved.
- Examine the virtual slides in the following table, identify draw and label the different blood cell types.
- Note whether the leucocytes or white blood cells (WBC) are granular or not.
- For each WBC, carefully note the shape of the nucleus, the relative amount of cytoplasm compared to the size of the nucleus, and the relative size of each cell type compared to erythrocytes or red blood cells (RBC).
The circulating blood is a liquid connective tissue consisting of cells (red and white blood cells), fragments of cells (platelets) and liquid (plasma). The different cell types are all derived from haemopoietic stem cells located in the bone marrow. Red blood cells (RBCs) have a metabolic role, in carrying oxygen to tissues and carbon dioxide to the lungs. White blood cells (WBCs or leukocytes) have a role in the body’s defence, and are an important clinical indicator of disease.
Scanning EM (coloured) of adult erythrocyte, thrombocyte and lymphocyte.
Showing relative sizes and morphologies. Remember a thrombocyte is not a cell, but circulating part or a fragment of a cell.
Virtual Slide Box: 1. Human Blood Smear Slide
Find an area in the smear where the red blood cells are spread out and individual cells can be identified.
- Red blood cells (7-8 um diameter anucleate biconcave disc)
- White blood cells: neutrophils, eosinophils, basophils, lymphocytes and monocytes (basophils are normally rare).
Note the presence or absence of granules, shape of the nucleus and relative cell sizes. Also identify platelets.
Virtual Slide Box: 2. Bone Marrow Smear Slide
- Do not attempt to identify all the cells in the bone marrow smear, but compare its appearance with that of the blood smear.
- Haematopoiesis (hematopoiesis) is the process of blood cell differentiation and occurs mainly in the bone marrow.
- This bone marrow smear will contain a large number of differentiating blood cells: band cells and normoblasts.
- The largest cells visible are megakaryocytes, which are responsible for platelet production.
Lymphocyte differentiation begins in the bone marrow and continues in central lymphoid organs (bone marrow - B cells and thymus - T cells), then in the peripheral lymphoid organs (lymph nodes, spleen).
|Common Histology Stains|
(Stain - Leishman) - Used to identify leucocytes and named after William Boog Leishman (1865 – 1926) was a Scottish pathologist.
- Methanol mixture of "polychromed" methylene blue (demethylated into various azures) and eosin.
- Methanol also acts as fixative.
- variations include Wrights Stain (America) and Giemsa and May-Grünwald stains in Germany and Europe.
Cell Histology Animation
| Simple animation with cells labeled to help identify HE stain appearance and relative sizes.
Use the RBCs as a ruler.
Blood Cell Numbers
Red Blood Cells
- Male: 4.32 - 5.66 x 1012/l
- Female: 3.88 - 4.99 x 1012/l
Leukocytes (white blood cells)
- Male: 3.7 - 9.5 x 109/l
- Female: 3.9 - 11.1 x 109/l
- 1.8 - 8.9 x 109/l
- Neutrophils: 1.5 - 7.4 x 109/l
- Eosinophils: 0.02 - 0.67 x 109/l
- Basophils: 0 - 0.13 x 109/l
- Monocytes 0.21 - 0.92 x 109/l
- 1.1 - 3.5 x 109/l
- B-cells: 0.06 - 0.66 x 109/l
- T-cells: 0.77 - 2.68 x 109/l
- CD4+: 0.53 - 1.76 x 109/l
- CD8+: 0.30 - 1.03 x 109/l
- NK cells: 0.20 - 0.40 x 109/l
- 140 - 440 x 109/l
- not a cell, a cell fragment.
| Cardiovascular System Development See also Heart terms, Immune terms and Blood terms.
|Other Terms Lists|
|Terms Lists: ART | Birth | Bone | Cardiovascular | Cell Division | Gastrointestinal | Genetic | Hearing | Heart | Immune | Integumentary | Neural | Oocyte | Palate | Placenta | Renal | Respiratory | Spermatozoa | Statistics | Ultrasound | Vision | Historic | Drugs | Glossary|
- Histology Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | ANAT2241 Support | Histology | Histology Stains | Embryology Glossary
|Common Histology Stains|
- Pages require student zpass to access.
The Virtual Microscope | Covering and Lining Epithelia | Glandular Epithelia | Connective Tissue Components | Connective Tissue Types | Bone, Bone Formation and Joints | Blood | Muscle Tissue | Nervous Tissue | Cardiovascular System | Respiratory System | Integumentary System (skin) | Gastro-Intestinal System I | Gastro-Intestinal System II | Liver, Gallbladder, and Pancreas | Lymphatic Tissue and Immune System | Endocrine System | Urinary System | Female Reproductive System | Male Reproductive System | Special Sense Organ: The Eye
- Pages can be accessed from any internet connected computer.
ANAT2241 Support Links: The Virtual Microscope | Covering and Lining Epithelia | Glandular Epithelia | CT Components | CT Types | Bone, Bone Formation and Joints | Blood | Muscle | Nervous | Cardiovascular | Respiratory | Integumentary | GIT 1 | GIT 2 | GIT Organs | Lymphatic and Immune | Endocrine | Urinary | Female Reproductive | Male Reproductive | Eye | Histology Stains | Histology Drawings | Practicals Health and Safety 2013
ANAT2241 This practical support page content is not part of the science practical class and provides only background information for student self-directed learning purposes.
Cite this page: Hill, M.A. (2018, March 21) Embryology ANAT2241 Blood. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/ANAT2241_Blood
- © Dr Mark Hill 2018, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G