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Lab Attendance

Lab 1 --Z3463514 (talk) 13:45, 7 August 2015 (AEST)

Lab 2 --Z3463514 (talk) 13:21, 14 August 2015 (AEST)

Lab 1 Assessment

Article 1 PMID 26131222

The aim of this article was to determine the impact of oxygen concentration during in vitro culture of human oocytes and embryo on fertilisation, implantation, pregnancy, gestation and abortion rates. Women between 20-48 years old who are seeking for infertility treatments and intracytoplasmic sperm injection participated in this experiment. Embryos were randomly allocated for incubation under three different oxygen conditions, the first group was subjected to 20% Oxygen in air. The second group was initially subjected to 20% Oxygen in air, then on the following day (day 2) 5% Carbon Dioxide and 90% Nitrogen gas was introduced into the system while Oxygen was decreased to 5%. Finally, the third group was subjected to 5% Carbon Dioxide gas and 90% Nitrogen gas throughout the experiment.

To determine its ability to yield a successful pregnancy, the embryo must be cultured and incubated for 2-6 days in a defined medium.

Embryos cultured in 5% Oxygen in air presented highest rates of fertilization and implantation compared to those incubated in 20% Oxygen in air. Meanwhile, embryos cultured in 20% Oxygen in air also presented high rates of fertilisation, high quality embryo and implantation. Intracytoplasmic sperm injection derived embryos cultured in 20% Oxygen in air resulted in lower rates of cleavage compared to those of from the second group, from 20% - 5% Oxygen in air. These results were consistent with the data previously published.

Article 2 PMID 26238449

The objective of this article was to evaluate in vitro maturation, IVM in sub-fertile women with polycystic ovarian syndrome undergoing IVF, by comparing outcomes with a control group of non-polycystic ovarian syndrome women. IVM involves the in vitro culture of immature oocytes from metaphase II, when the oocyte is considered to be fully mature to undergo fertilisation. This could be a groundbreaking procedure for women suffering polycystic ovarian syndrome as these gametes has expressed their maturational and developmental competence after their retrieval from the ovaries.

A total of 268 patients suffering with polycystic ovarian syndrome, 100 PCO patients and 400 controls were included in the investigation. The analysis provided information suggesting that IVM is a preferable approach in treating women with PCOS during an IVF cycle compared to those without the syndrome. Thus is was concluded that IVM was more efficient as a treatment option in terms of clinical pregnancy, implantation and cycle cancellation rate in women suffering PCOS.

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2015 Course: Week 2 Lecture 1 Lecture 2 Lab 1 | Week 3 Lecture 3 Lecture 4 Lab 2 | Week 4 Lecture 5 Lecture 6 Lab 3 | Week 5 Lecture 7 Lecture 8 Lab 4 | Week 6 Lecture 9 Lecture 10 Lab 5 | Week 7 Lecture 11 Lecture 12 Lab 6 | Week 8 Lecture 13 Lecture 14 Lab 7 | Week 9 Lecture 15 Lecture 16 Lab 8 | Week 10 Lecture 17 Lecture 18 Lab 9 | Week 11 Lecture 19 Lecture 20 Lab 10 | Week 12 Lecture 21 Lecture 22 Lab 11 | Week 13 Lecture 23 Lecture 24 Lab 12 | 2015 Projects: Three Person Embryos | Ovarian Hyper-stimulation Syndrome | Polycystic Ovarian Syndrome | Male Infertility | Oncofertility | Preimplantation Genetic Diagnosis | Students | Student Designed Quiz Questions | Moodle page

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