Somatic Cell Nuclear Transfer: Difference between revisions

Revision as of 13:08, 22 August 2012

Introduction

Page Under Development

Dolly the Sheep

In 1996 Dolly the sheep was the first animal to be produced by somatic cell nuclear transfer (SCNT) using an adult-derived somatic cell as nuclear donor. A somatic cell refers to the fact that a cell that is not a germ cell (spermatozoa, oocyte) is used to generate a zygote from which the embryo develops.

A range of different cell types has now been successfully applied to a range of species (cattle, mice, goats, pigs, cats, rabbits, horses, rats, dogs and ferrets. (see review^[1])

Some Recent Findings

Number of blastomeres and distribution of microvilli in cloned mouse embryos during compaction^[2] "We concluded that: (i) the cleavage of blastomeres in cloned embryos was slow at least before compaction; (ii) the distribution of microvilli in cloned, normal, parthenogenetic, and tetraploid embryos was coherent before and after compaction; and (iii) the initiation of compaction in somatic cell nuclear transfer (SCNT) embryos was delayed compared with that of intracytoplasmic sperm injection (ICSI) embryos."

Animal Timeline

1996 - Sheep^[3]
1998 - Cattle^[4], Mouse^[5]
1999 - Goat^[6]
2000 - Pig^[7]

References

↑ <pubmed>22681293</pubmed>
↑ <pubmed>20735894</pubmed>
↑ <pubmed>8598906</pubmed>
↑ <pubmed>9596577</pubmed>
↑ <pubmed>9690471</pubmed>
↑ <pubmed>10331804</pubmed>
↑ <pubmed>10993078</pubmed>

Journals

Zygote An international journal dedicated to the rapid publication of original research in early embryology, Zygote covers interdisciplinary studies in animals and humans, from gametogenesis through fertilization to gastrulation.

Reviews

Articles

Search Pubmed

June 2010 "zygote" All (6213) Review (651) Free Full Text (1543)

Search Pubmed: somatic cell nuclear transfer

Glossary Links

Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link

Cite this page: Hill, M.A. (2024, April 26) Embryology Somatic Cell Nuclear Transfer. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Somatic_Cell_Nuclear_Transfer

What Links Here?

[1] <pubmed>22681293</pubmed>

[2] <pubmed>20735894</pubmed>

[3] <pubmed>8598906</pubmed>

[4] <pubmed>9596577</pubmed>

[5] <pubmed>9690471</pubmed>

[6] <pubmed>10331804</pubmed>

[7] <pubmed>10993078</pubmed>

[1]

[2]

[3]

[4]

[5]

[6]

[7]

@@ Line 1: / Line 1: @@
 ==Introduction==
 '''''Page Under Development'''''
+[[File:Dolly the sheep.jpg|thumb|Dolly the Sheep]]
-[[File:Early zygote.jpg|thumb|300px|Early Human Zygote, with 2 pronuclei]]
+In 1996 Dolly the sheep was the first animal to be produced by somatic cell nuclear transfer (SCNT) using an adult-derived somatic cell as nuclear donor. A somatic cell refers to the fact that a cell that is not a germ cell (spermatozoa, oocyte) is used to generate a zygote from which the embryo develops.
-The first diploid cell that forms following [[fertilization]] by fusion of the haploid [[oocyte]] (egg) and [[spermatozoa]] (sperm) resulting in the combination of their separate genomes. The first image shows the cell with the 2 pronuclei still present before fusion enclosed within the zona pellucida. In humans, this cell then undergoes a series of mitotic cell divisions, still enclosed within the zona pellucida, during the first few days of development to form 2 blastomeres then a solid cell mass called the [[morula]].
-:[[Somatic Cell Nuclear Transfer|'''SCNT Links''': [[Somatic Cell Nuclear Transfer|Introduction]] | [[Stem_Cells_-_Adult#Somatic_Cell_Nuclear_Transfer|Stem Cells - SCNT]] | [[Assisted Reproductive Technology|ART]] | [[Fertilization]] | [[Week 1]] | [[Morula]] | [[Blastocyst]] | [[Assisted Reproductive Technology|ART]] | [[:Category:Zygote|Category:Zygote]]
+A range of different cell types has now been successfully applied to a range of species (cattle, mice, goats, pigs, cats, rabbits, horses, rats, dogs and ferrets. (see review<ref><pubmed>22681293</pubmed></ref>)
+:[[Somatic Cell Nuclear Transfer|'''SCNT Links''': [[Somatic Cell Nuclear Transfer|Introduction]] | [[Stem_Cells_-_Adult#Somatic_Cell_Nuclear_Transfer|Stem Cells - SCNT]] | [[Molecular_Development_-_Epigenetics|Epigenetics]] | [[Stem Cells]] | [[Assisted Reproductive Technology|ART]] | [[Fertilization]] | [[Week 1]] | [[:Category:Zygote|Category:Zygote]]
@@ Line 17: / Line 19: @@
-[[Molecular_Development_-_Epigenetics|Epigenetics]]
+===Animal Timeline===
+* '''1996''' - Sheep<ref><pubmed>8598906</pubmed></ref>
+* '''1998''' - Cattle<ref><pubmed>9596577</pubmed></ref>, Mouse<ref><pubmed>9690471</pubmed></ref>
+* '''1999''' - Goat<ref><pubmed>10331804</pubmed></ref>
+* '''2000''' - Pig<ref><pubmed>10993078</pubmed></ref>
@@ Line 36: / Line 43: @@
 June 2010 "zygote" All (6213) Review (651) Free Full Text (1543)
-'''Search Pubmed:''' [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=zygote zygote]
+'''Search Pubmed:''' [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=somatic+cell+nuclear+transfer somatic cell nuclear transfer]