Revision as of 01:48, 17 September 2009

Muscle Development

Introduction

This laboratory will look at the musculoskeletal development. The laboratory will also allow time for work on the group online project.

Objectives

Understand the origin, differentiation and development of skeletal muscle tissue.
Know what is meant by patterning, conversion and adult plasticity of muscle fibre type.
Develop an undestanding of research methods for studying fibre type control.

Lab Tutorial

Today's laboratory will begin with a tutorial presentation on muscle development and research into muscle disorders. During the presentation feel free to ask questions about any concepts you may not understand.

Slides: Muscle Development - Part 1 | Muscle Development - Part 2

Presentation Slides 4 / page (printing version): Muscle Development - Part 1 | Muscle Development - Part 1

Skeletal Muscle Histology

Lab 7 Assessment Questions

Answer the questions shown below on your own student page.

Briefly; what is a myotubeand how is it formed?
What changes would I expect to see in the muscle fibre types in my legs if I:

a) Suffered a spinal cord injury

b) Took up marathon running

Questions are also shown listed on the Students Page Lab 7 Assessment

References - Dr Palmer

Expression of Gtf2ird1, the Williams syndrome-associated gene, during mouse development. Palmer SJ, Tay ES, Santucci N, Cuc Bach TT, Hook J, Lemckert FA, Jamieson RV, Gunnning PW, Hardeman EC. Gene Expr Patterns. 2007 Feb;7(4):396-404. PMID: 17239664

MusTRD can regulate postnatal fiber-specific expression. Issa LL, Palmer SJ, Guven KL, Santucci N, Hodgson VR, Popovic K, Joya JE, Hardeman EC. Dev Biol. 2006 May 1;293(1):104-15. PMID: 16494860

hMusTRD1alpha1 represses MEF2 activation of the troponin I slow enhancer. Polly P, Haddadi LM, Issa LL, Subramaniam N, Palmer SJ, Tay ES, Hardeman EC. J Biol Chem. 2003 Sep 19;278(38):36603-10. PMID: 12857748

Search

Bookshelf muscle development | muscle fiber type | troponin

Pubmed muscle fiber type | troponin | Gtf2ird1 |

Links

Blue Histology Skeletal Muscle histology
Primer Skeletal Muscle Fiber Type: Influence on Contractile and Metabolic Properties
Wiki Skeletal muscle | Troponin
OMIM: Gtf2ird1
OMIM: William-Beuren syndrome

Terms

enhancer - A cis-regulatory sequence that can regulate levels of transcription from an adjacent promoter. Many tissue-specific enhancers can determine spatial patterns of gene expression in higher eukaryotes. Enhancers can act on promoters over many tens of kilobasesof DNA and can be 5' or 3' to the promoter they regulate. Gtf2ird1 - General Transcription Factor 2 -I Repeat domain-containing protein 1

MusTRD1 - muscle TFII-I repeat domain-containing protein 1

promoter - A regulatory region a short distance upstream from the 5' end of a transcription start site that acts as the binding site for RNA polymerase II. A region of DNA to which RNA polymerase IIbinds in order to initiate transcription.

Troponin - striated muscle contraction is regulated by the calcium-ion-sensitive, multiprotein complex troponin and the fibrous protein tropomyosin. Troponin has 3 subunits (TnC, TnI, TnT) and is located on the actin filament. OMIM: Troponin I slow

UNSW Embryology Links

Glossary Links

Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link

Course Content 2009

Cite this page: Hill, M.A. (2024, May 6) Embryology 2009 Lab 7. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/2009_Lab_7

What Links Here?

@@ Line 65: / Line 65: @@
 == Terms ==
+'''enhancer''' - A cis-regulatory sequence that can regulate levels of transcription from an adjacent promoter. Many tissue-specific enhancers can determine spatial patterns of gene expression in higher eukaryotes. Enhancers can act on promoters over many tens of kilobasesof DNA and can be 5' or 3' to the promoter they regulate.
 '''Gtf2ird1''' - General Transcription Factor 2 -I Repeat domain-containing protein 1
 '''MusTRD1'''  - muscle TFII-I repeat domain-containing protein 1
+'''promoter''' - A regulatory region a short distance upstream from the 5' end of a transcription start site that acts as the binding site for RNA polymerase II. A region of DNA to which RNA polymerase IIbinds in order to initiate transcription.
 '''Troponin''' - striated muscle contraction is regulated by the calcium-ion-sensitive, multiprotein complex troponin and the fibrous protein tropomyosin. Troponin has 3 subunits (TnC, TnI, TnT) and is located on the actin filament. [http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191040 OMIM: Troponin I slow]

2009 Lab 7: Difference between revisions