ANAT2341 Lab 7
Muscle Development
Introduction
This laboratory concerns the development and differentiation of skeletal muscle, muscle stem cells, fibre type differentiation and muscle disease.
Objectives
- Understand the origin, differentiation and development of skeletal muscle tissue.
- Know what is meant by patterning, conversion and adult plasticity of muscle fibre type.
- Develop an understanding of research methods for studying skeletal muscle abnormalities.
Lab Exercises
Lab 7 Assessment Questions
TBA
Search
- Bookshelf muscle development | muscle fiber type | troponin
- Pubmed muscle fiber type | troponin | Gtf2ird1 |
UNSW Embryology Links
- File:Muscle development 1-Talk1 muscle development.pdf
- File:Talk1 muscle development 4 per page.pdf
- File:Part 2 Muscle Development.pdf
- File:Part 2 Muscle Development 4 per page.pdf
Terms
anterior tibialis - (tibialis anterior) skeletal muscle situated on the lateral side of the tibia and is a direct flexor of the foot at the ankle-joint.
cis-acting elements - DNA sequences that through transcription factors or other trans-acting elements or factors, regulate the expression of genes on the same chromosome.
enhancer - A cis-regulatory sequence that can regulate levels of transcription from an adjacent promoter. Many tissue-specific enhancers can determine spatial patterns of gene expression in higher eukaryotes. Enhancers can act on promoters over many tens of kilobases of DNA and can be 5' or 3' to the promoter they regulate.
extensor digitorum longus - (EDL) skeletal muscle situated at the lateral part of the front of the leg and extend the phalanges of the toes, and, continuing their action, flex the foot upon the leg.
Gtf2ird1 - General Transcription Factor 2 -I Repeat domain-containing protein 1. OMIM: Gtf2ird1
MusTRD1 - muscle TFII-I repeat domain-containing protein 1.
MyHC - acronym for myosin heavy chain.
myoblast - the undifferentiated mononucleated muscle cell progenitor, which in skeletal muscle fuses to form a myotube, that in turn expresses contractile proteins to form a muscle fibre.
myosin heavy chain - protein forming the thick filament of the sarcomere and the motor for actin-myosin contraction. There are 17 different myosin classes.
myotube - the initial multinucleated cell formed by fusion of myoblasts during skeletal muscle development.
promoter - A regulatory region a short distance upstream from the 5' end of a transcription start site that acts as the binding site for RNA polymerase II. A region of DNA to which RNA polymerase IIbinds in order to initiate transcription.
regulatory sequence - (regulatory region, regulatory area) is a segment of DNA where regulatory proteins such as transcription factors bind preferentially.
soleus - skeletal muscle situated immediately in front of the gastrocnemius and when standing taking its fixed point from below, steadies the leg upon the foot and prevents the body from falling forward.
Troponin - striated muscle contraction is regulated by the calcium-ion-sensitive, multiprotein complex troponin and the fibrous protein tropomyosin. Troponin has 3 subunits (TnC, TnI, TnT) and is located on the actin filament. OMIM: Troponin I slow
visuospatial deficiency - performing the Delis hierarchical processing task, subjects are asked to copy a large global figure made of smaller local forms. Both Down syndrome (DS) and William-Beuren syndrome (WBS) groups fail but in different ways. WBS individuals produce the local elements and DS individuals produce only the global forms. PMID: 12952863 Hum Mol Genet.
William-Beuren syndrome - (WBS) rare developmental disorder (1/20,000–1/50,000 live births). A contiguous gene deletion syndrome resulting from the hemizygous deletion of several genes on chromosome 7q11.23. The syndrome has associated craniofacial abnormalities, hypersociability and visuospatial defects. OMIM: William-Beuren syndrome
External Links
External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name. Links to any external commercial sites are provided for information purposes only and should never be considered an endorsement. UNSW Embryology is provided as an educational resource with no clinical information or commercial affiliation.
- Blue Histology Skeletal Muscle histology
- Primer Skeletal Muscle Fiber Type: Influence on Contractile and Metabolic Properties
- Gray's Anatomy of the Human Body - The Muscles and Fasciæ of the Leg | Cross-section through middle of leg
- Wiki Skeletal muscle | Troponin
- OMIM: Gtf2ird1
- OMIM: William-Beuren syndrome
Glossary Links
- Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link
Cite this page: Hill, M.A. (2026, October 16) Embryology ANAT2341 Lab 7. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/ANAT2341_Lab_7
- © Dr Mark Hill 2026, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G
References
| ANAT2341 Course Timetable | |||
|---|---|---|---|
| Week (Mon) | Lecture 1 (Mon 1-2pm) | Lecture 2 (Tue 3-4pm) | Practical (Fri 1-3pm) |
| Week 2 (1 Aug) | Introduction | Fertilization | Lab 1 |
| Week 3 (8 Aug) | Week 1 and 2 | Week 3 | Lab 2 |
| Week 4 (15 Aug) | Mesoderm | Ectoderm | Lab 3 |
| Week 5 (22 Aug) | Early Vascular | Placenta | Lab 4 |
| Week 6 (29 Aug) | Gastrointestinal | Respiratory | Lab 5 |
| Week 7 (5 Sep) | Head | Neural Crest | Lab 6 |
| Week 8 (12 Sep) | Musculoskeletal | Limb Development | Lab 7 |
| Week 9 (19 Sep) | Renal | Genital | Lab 8 |
| Mid-semester break | |||
| Week 10 (3 Oct) | Public Holiday | Stem Cells | Lab 9 |
| Week 11 (10 Oct) | Integumentary | Endocrine | Lab 10 |
| Week 12 (17 Oct) | Heart | Sensory | Lab 11 |
| Week 13 (24 Oct) | Fetal | Birth and Revision | Lab 12 |
|
ANAT2341 2016: Moodle page | ECHO360 | Textbooks | Students 2016 | Projects 2016 | |||
