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==Lab 4 Assessment - Quiz Questions== | ==Lab 4 Assessment - Quiz Questions== | ||
<b> Early Vascular Development </b> | |||
<quiz display=simple> | |||
{Which of the following statement is correct about angiogenesis: | |||
|type="()"} | |||
- Begins week in extra embryonic mesoderm and then embryonic splanchnic mesoderm | |||
- Begins as the formation of blood islands | |||
- Blood islands extend and fuse together to form a primordial vascular network | |||
- VEGF and PGF stimulates growth and development | |||
+ All of the above | |||
||Yes, angiogenesis is a vital process in growth and development as well as in wound healing as it is the physiological process for the formation of new blood vessels from existing vessels. | |||
{Which of the following statement is NOT correct about blood vessel remodelling: | |||
|type="()"} | |||
- The branches from main arteries may arise as new outgrowths from the enlarged stem | |||
- VEGF is required later for endothelial cell maintenance in tissues | |||
- Extensive remodelling during embryo development leads to an asymmetrical adult system in the body | |||
+ Early vascular development is asymmetrical | |||
||During early vascular development it is laterally symmetrical. | |||
{What cells are not contained in blood islands? | |||
|type="()"} | |||
+ Blastocyst | |||
- Harmoangioblasts | |||
- Angioblasts | |||
- Haemocytoblasts | |||
||Blood island contains haemangioblasts which have the ability to differentiate into angioblasts and harmocytoblasts which are vascular and blood cell precursors respectively. | |||
</quiz> | |||
Revision as of 17:34, 3 September 2015
Lab Attendance
Lab 1 --Z3463514 (talk) 13:45, 7 August 2015 (AEST)
Lab 2 --Z3463514 (talk) 13:21, 14 August 2015 (AEST)
Lab 3 --Z3463514 (talk) 12:28, 21 August 2015 (AEST)
Lab 4 --Z3463514 (talk) 12:12, 28 August 2015 (AEST)
Lab 1 Assessment
Article 1 PMID 26131222
The aim of this article was to determine the impact of oxygen concentration during in vitro culture of human oocytes and embryo on fertilisation, implantation, pregnancy, gestation and abortion rates. Women between 20-48 years old who are seeking for infertility treatments and intracytoplasmic sperm injection participated in this experiment. Embryos were randomly allocated for incubation under three different oxygen conditions, the first group was subjected to 20% Oxygen in air. The second group was initially subjected to 20% Oxygen in air, then on the following day (day 2) 5% Carbon Dioxide and 90% Nitrogen gas was introduced into the system while Oxygen was decreased to 5%. Finally, the third group was subjected to 5% Carbon Dioxide gas and 90% Nitrogen gas throughout the experiment.
To determine its ability to yield a successful pregnancy, the embryo must be cultured and incubated for 2-6 days in a defined medium.
Embryos cultured in 5% Oxygen in air presented highest rates of fertilization and implantation compared to those incubated in 20% Oxygen in air. Meanwhile, embryos cultured in 20% Oxygen in air also presented high rates of fertilisation, high quality embryo and implantation. Intracytoplasmic sperm injection derived embryos cultured in 20% Oxygen in air resulted in lower rates of cleavage compared to those of from the second group, from 20% - 5% Oxygen in air. These results were consistent with the data previously published.
Article 2 PMID 26238449
The objective of this article was to evaluate in vitro maturation, IVM in sub-fertile women with polycystic ovarian syndrome undergoing IVF, by comparing outcomes with a control group of non-polycystic ovarian syndrome women. IVM involves the in vitro culture of immature oocytes from metaphase II, when the oocyte is considered to be fully mature to undergo fertilisation. This could be a groundbreaking procedure for women suffering polycystic ovarian syndrome as these gametes has expressed their maturational and developmental competence after their retrieval from the ovaries.
A total of 268 patients suffering with polycystic ovarian syndrome, 100 PCO patients and 400 controls were included in the investigation. The analysis provided information suggesting that IVM is a preferable approach in treating women with PCOS during an IVF cycle compared to those without the syndrome. Thus is was concluded that IVM was more efficient as a treatment option in terms of clinical pregnancy, implantation and cycle cancellation rate in women suffering PCOS.
Lab 2 Assessment - Images
Uploading Images in 5 Easy Steps | ||
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First Read the help page Images and Copyright Tutorial.
Students cannot delete images once uploaded. You will need to email me with the full image name and request deletion, that I am happy to do with no penalty if done before I assess. Non-Table version of this page
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Deer mice oocytes at various stages of development in vitro [1]
PMID 23457518
Copyright: © 2013 Choi, He. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Lab 4 Assessment - Quiz Questions
Early Vascular Development
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