Molecular Development - microRNA: Difference between revisions
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* '''MicroRNA Expression during Bovine Oocyte Maturation and Fertilization'''<ref name=PMID26999121><pubmed>26999121</pubmed>| [http://www.mdpi.com/1422-0067/17/3/396 Int J Mol Sci.]</ref> "In order to further explore the roles of miRNAs in oocyte maturation, we employed small RNA sequencing as a screening tool to identify and characterize miRNA populations present in pools of bovine germinal vesicle (GV) oocytes, metaphase II (MII) oocytes, and presumptive zygotes (PZ). Each stage contained a defined miRNA population, some of which showed stable expression while others showed progressive changes between stages that were subsequently confirmed by quantitative reverse transcription polymerase chain reaction (RT-PCR). Bta-miR-155, bta-miR-222, bta-miR-21, bta-let-7d, bta-let-7i, and bta-miR-190a were among the statistically significant differentially expressed miRNAs (p < 0.05). To determine whether changes in specific primary miRNA (pri-miRNA) transcripts were responsible for the observed miRNA changes, we evaluated pri-miR-155, -222 and let-7d expression. Pri-miR-155 and -222 were not detected in GV oocytes but pri-miR-155 was present in MII oocytes, indicating transcription during maturation. In contrast, levels of pri-let-7d decreased during maturation, suggesting that the observed increase in let-7d expression was likely due to processing of the primary transcript. This study demonstrates that both dynamic and stable populations of miRNAs are present in bovine oocytes and zygotes and extend previous studies supporting the importance of the small RNA landscape in the maturing bovine oocyte and early embryo." [[Bovine Development]] | |||
* '''Maternal peripheral blood natural killer cells incorporate placenta-associated microRNAs during pregnancy'''<ref name=PMID24682221><pubmed>24682221</pubmed>| [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432927 PMC4432927] | [http://www.spandidos-publications.com/ijmm/35/6/1511 Int J Mol Med.}</ref> "Although recent studies have demonstrated that microRNAs (miRNAs or miRs) regulate fundamental natural killer (NK) cellular processes, including cytotoxicity and cytokine production, little is known about the miRNA‑gene regulatory relationships in maternal peripheral blood NK (pNK) cells during pregnancy. ...Twenty‑five miRNAs, including six C19MC miRNAs, were significantly upregulated in the third‑ compared to first‑trimester pNK cells. The rapid clearance of C19MC miRNAs also occurred in the pNK cells following delivery. Nine miRNAs, including eight C19MC miRNAs, were significantly downregulated in the post‑delivery pNK cells compared to those of the third‑trimester. DNA microarray analysis identified 69 NK cell function‑related genes that were differentially expressed between the first‑ and third‑trimester pNK cells. On pathway and network analysis, the observed gene expression changes of pNK cells likely contribute to the increase in the cytotoxicity, as well as the cell cycle progression of third‑ compared to first‑trimester pNK cells. Thirteen of the 69 NK cell function‑related genes were significantly downregulated between the first‑ and third‑trimester pNK cells. Nine of the 13 downregulated NK‑function‑associated genes were in silico target candidates of 12 upregulated miRNAs, including C19MC miRNA miR‑512‑3p. The results of this study suggest that the transfer of placental C19MC miRNAs into maternal pNK cells occurs during pregnancy." | * '''Maternal peripheral blood natural killer cells incorporate placenta-associated microRNAs during pregnancy'''<ref name=PMID24682221><pubmed>24682221</pubmed>| [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432927 PMC4432927] | [http://www.spandidos-publications.com/ijmm/35/6/1511 Int J Mol Med.}</ref> "Although recent studies have demonstrated that microRNAs (miRNAs or miRs) regulate fundamental natural killer (NK) cellular processes, including cytotoxicity and cytokine production, little is known about the miRNA‑gene regulatory relationships in maternal peripheral blood NK (pNK) cells during pregnancy. ...Twenty‑five miRNAs, including six C19MC miRNAs, were significantly upregulated in the third‑ compared to first‑trimester pNK cells. The rapid clearance of C19MC miRNAs also occurred in the pNK cells following delivery. Nine miRNAs, including eight C19MC miRNAs, were significantly downregulated in the post‑delivery pNK cells compared to those of the third‑trimester. DNA microarray analysis identified 69 NK cell function‑related genes that were differentially expressed between the first‑ and third‑trimester pNK cells. On pathway and network analysis, the observed gene expression changes of pNK cells likely contribute to the increase in the cytotoxicity, as well as the cell cycle progression of third‑ compared to first‑trimester pNK cells. Thirteen of the 69 NK cell function‑related genes were significantly downregulated between the first‑ and third‑trimester pNK cells. Nine of the 13 downregulated NK‑function‑associated genes were in silico target candidates of 12 upregulated miRNAs, including C19MC miRNA miR‑512‑3p. The results of this study suggest that the transfer of placental C19MC miRNAs into maternal pNK cells occurs during pregnancy." | ||
* '''Potential Regulatory Role of MicroRNAs in the Development of Bovine Gastrointestinal Tract during Early Life'''<ref name=PMID24682221><pubmed>24682221</pubmed>| [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0092592 PLoS One.]</ref> "This study aimed to investigate the potential regulatory role of miRNAs in the development of gastrointestinal tract (GIT) during the early life of dairy calves. Rumen and small intestinal (mid-jejunum and ileum) tissue samples were collected from newborn (30 min after birth; n = 3), 7-day-old (n = 6), 21-day-old (n = 6), and 42-day-old (n = 6) dairy calves. The miRNA profiling was performed using Illumina RNA-sequencing and the temporal and regional differentially expressed miRNAs were further validated using qRT-PCR. ...The present study revealed temporal and regional changes in miRNA expression and a correlation between miRNA expression and microbial population in the GIT during the early life, which provides further evidence for another mechanism by which host-microbial interactions play a role in regulating gut development." | * '''Potential Regulatory Role of MicroRNAs in the Development of Bovine Gastrointestinal Tract during Early Life'''<ref name=PMID24682221><pubmed>24682221</pubmed>| [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0092592 PLoS One.]</ref> "This study aimed to investigate the potential regulatory role of miRNAs in the development of gastrointestinal tract (GIT) during the early life of dairy calves. Rumen and small intestinal (mid-jejunum and ileum) tissue samples were collected from newborn (30 min after birth; n = 3), 7-day-old (n = 6), 21-day-old (n = 6), and 42-day-old (n = 6) dairy calves. The miRNA profiling was performed using Illumina RNA-sequencing and the temporal and regional differentially expressed miRNAs were further validated using qRT-PCR. ...The present study revealed temporal and regional changes in miRNA expression and a correlation between miRNA expression and microbial population in the GIT during the early life, which provides further evidence for another mechanism by which host-microbial interactions play a role in regulating gut development." |
Revision as of 13:10, 30 March 2016
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Introduction
Micro RNA (miRNA) are 22 nucleotide non-coding RNAs. These small pieces of RNA have been identified as important negative regulators in both development and adult cell processes involving gene expression.
Some Recent Findings
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More recent papers |
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This table allows an automated computer search of the external PubMed database using the listed "Search term" text link.
More? References | Discussion Page | Journal Searches | 2019 References | 2020 References Search term: microRNA Embryology <pubmed limit=5>microRNA Embryology</pubmed> |
Functional miRNA Grouping
The following identifies microRNAs that have been identified with specific developmental processes, based upon a commercial collation of basic research data.
- Pluripotency
- let-7a, let-7b, let-7c, let-7d, let-7e, let-7g, miR-101, miR-106b, miR-125b, miR-130a, miR-133b, miR-141, miR-15a, miR-17, miR-182, miR-183, miR-18a, miR-18b, miR-205, miR-20a, miR-20b, miR-21, miR-214, miR-22, miR-222, miR-23b, miR-24, miR-302a, miR-302c, miR-345, miR-424, miR-498, miR-518b, miR-520g.
Early Development
- Embryoid Bodies
- miR-132, miR-181a, miR-9.
- Definitive Endoderm
- miR-205, miR-375.
Ectoderm
- Neural Development
- let-7b, miR-103a, miR-106b, miR-10b, miR-124, miR-125b, miR-130a, miR-132, miR-134, miR-137, miR-16, miR-181a, miR-182, miR-183, miR-20a, miR-210, miR-219-5p, miR-22, miR-23b, miR-24, miR-26a, miR-302a, miR-302c, miR-7, miR-9, miR-96.
- Eye Development
- miR-130a, miR-196a, miR-219-5p, miR-23b, miR-96.
- Epidermal Differentiation
- let-7b, miR-205, miR-210, miR-23b, miR-26a.
- Inner Ear Development
- miR-182, miR-183, miR-96.
Mesoderm
- Haematopoiesis
- let-7e, miR-125a-5p, miR-142-3p, miR-223.
- T Cell Development
- let-7a, let-7f, miR-106b, miR-142-5p, miR-146b-5p, miR-150, miR-15a, miR-15b, miR-16, miR-181a, miR-20a, miR-222, miR-26a.
- Erythropoiesis
- let-7a, let-7b, let-7c, let-7d, let-7f, let-7g, let-7i, miR-126, miR-128a, miR-137, miR-155, miR-15b, miR-16, miR-17, miR-181a, miR-182, miR-185, miR-206, miR-21, miR-22, miR-222, miR-24, miR-26a, miR-96.
- Lymphopoiesis
- let-7b, miR-125b, miR-128a, miR-16, miR-181a, miR-21, miR-24.
- Megakaryopoiesis
- miR-106b, miR-10a, miR-10b, miR-122, miR-126, miR-127-5p, miR-129-5p, miR-134, miR-146a, miR-150, miR-155, miR-17, miR-18a, miR-192, miR-20a, miR-20b, miR-21, miR-22, miR-301a, miR-33a, miR-378, miR-92a, miR-93.
- Monocyte Differentiation
- miR-155, miR-222, miR-424.
- Myelopoiesis
- miR-103a, miR-128a, miR-17, miR-181a, miR-24.
- Angiogenesis
- miR-126, miR-130a, miR-218, miR-222, miR-92a.
- Myogenesis
- miR-1, miR-125b, miR-206, miR-26a.
- Osteogenesis
- miR-141, miR-15b, miR-424.
- Adipogenesis
- let-7b, let-7c, let-7e, miR-100, miR-101, miR-103a, miR-10b, miR-146b-5p, miR-155, miR-182, miR-192, miR-194, miR-196a, miR-21, miR-210, miR-214, miR-22, miR-24, miR-498, miR-96, miR-99a.
- Chondrogenesis: let-7f, miR-1, miR-132, miR-181a, miR-196a, miR-96, miR-99a.
- Heart Development: miR-1, miR-208, miR-488.
Endoderm
- Liver Development
- let-7a, let-7b, let-7c, miR-10a, miR-122, miR-125b, miR-192, miR-21, miR-22, miR-23b, miR-92a, miR-99a.
- Pancreatic Development
- miR-15a, miR-15b, miR-16, miR-195, miR-214, miR-375, miR-7, miR-9.
- Intestinal Development
- let-7d, let-7e, miR-103a, miR-106b, miR-125b, miR-126, miR-130a, miR-141, miR-146b-5p, miR-17, miR-192, miR-194, miR-21, miR-215, miR-301a, miR-424.
- Data: SABiosciences Cell Differentiation & Development miRNA PCR Array
Nucleolus
Five miRNAs show highest nucleolar concentration in myoblasts using the microarray assay, miR-340-5p, miR-351, miR-494, miR-664, or let-7e, are thought to be skeletal muscle-specific miRNAs (i.e., miR-1, miR-133, and miR-206 and perhaps miR-95, miR-128a, and miR-499).[4]
References
- ↑ 1.0 1.1 1.2 <pubmed>24682221</pubmed>| PLoS One. Cite error: Invalid
<ref>
tag; name 'PMID24682221' defined multiple times with different content Cite error: Invalid<ref>
tag; name 'PMID24682221' defined multiple times with different content - ↑ <pubmed>26999121</pubmed>| Int J Mol Sci.
- ↑ <pubmed>21989093</pubmed>
- ↑ <pubmed>19628621</pubmed>
Reviews
<pubmed>21850044</pubmed> <pubmed>21742789</pubmed> <pubmed>21576351</pubmed> <pubmed>21504869</pubmed> <pubmed>21486922</pubmed> <pubmed>19148191</pubmed>
Articles
<pubmed></pubmed> <pubmed></pubmed> <pubmed>23617334</pubmed> <pubmed>20520778</pubmed> <pubmed>18029362</pubmed>| Nucleic Acids Res. <pubmed></pubmed>
Search Pubmed
Search Pubmed Now: microRNA
External Links
External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name. Links to any external commercial sites are provided for information purposes only and should never be considered an endorsement. UNSW Embryology is provided as an educational resource with no clinical information or commercial affiliation.
- Memorial Sloan-Kettering Cancer Center - microRNA.org - Targets and Expression
- SABiosciences - Cell Differentiation & Development miRNA PCR Array
- Sigma-Aldrich - miRNA Introduction
- miRNAMap - http://mirnamap.mbc.nctu.edu.tw/
Glossary Links
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Cite this page: Hill, M.A. (2024, June 26) Embryology Molecular Development - microRNA. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Molecular_Development_-_microRNA
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G